scholarly journals A bioassay system of autologous human endothelial, smooth muscle cells, and leukocytes for use in drug discovery, phenotyping, and tissue engineering

2019 ◽  
Vol 34 (1) ◽  
pp. 1745-1754 ◽  
Author(s):  
Blerina Ahmetaj‐Shala ◽  
Ryota Kawai ◽  
Isra Marei ◽  
Zacharoula Nikolakopoulou ◽  
Chih‐Chin Shih ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Drug Discovery ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Bioassay System

Surface Engineering in Microfluidic Devices for the Isolation of Smooth Muscle Cells and Endothelial Cells

2007 ◽  
Vol 1004 ◽  
Author(s):  
Shashi Murthy ◽  
Brian Plouffe ◽  
Milica Radisic
Keyword(s):  
Tissue Engineering ◽  
Endothelial Cells ◽  
Shear Stress ◽  
Smooth Muscle ◽  
Cell Adhesion ◽  
Smooth Muscle Cells ◽  
Cell Sorting ◽  
Microfluidic Devices ◽  
Muscle Cells ◽  
Small Sample

AbstractMicrofluidic cell separation systems have emerged as attractive alternatives to traditional techniques in recent years. These systems offer the advantages of being able to handle small sample volumes and at the same time achieve highly selective separation. Conventional separation techniques, including both fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS), typically require a pre-processing incubation step to attach ligated tags (such as fluorescent dyes or magnetic beads) to cell surfaces prior to separation. These techniques are also constrained by infrastructure and high cost. Microfluidic devices with surface-immobilized adhesion molecules eliminate the need for pre-processing incubation and are a low cost alternative.We describe the selective adhesion of smooth muscle cells and endothelial cells in microfluidic devices coated with adhesion peptides. The device geometry is such that the shear stress varies linearly as a function of flow channel length, allowing simultaneous evaluation of the effects of surface chemistry and fluid shear on cell adhesion. The adhesion peptides, val-ala-pro-gly (VAPG) and arg-glu-asp-val (REDV), are known to bind selectively to smooth muscle cells and endothelial cells, respectively. These peptides were tethered to the device surface using silane chemistry and NHS-ester coupling. Cell adhesion was examined in a shear stress range of 1.3-4.0 dyn/cm2. Under these conditions, endothelial cells show significantly higher adhesion to REDV-coated devices compared to smooth muscle cells and fibroblasts. Correspondingly, smooth muscle cell adhesion in VAPG-coated devices is much greater than that of endothelial cells and fibroblasts. This selective binding behavior is also observed when mixed suspensions of the three cell types are flowed into both types of peptide-coated microfluidic devices. These results suggest that microfluidic devices coated with REDV and VAPG can be used as effective separation tools in various applications, such as tissue engineering. Specific examples of applications in cardiac and skin tissue engineering will be discussed.

scholarly journals Comparative analysis of myometrial and vascular smooth muscle cells to determine optimal cells for use in drug discovery

2019 ◽  
Vol 146 ◽  
pp. 104268 ◽  
Author(s):  
Shajila Siricilla ◽  
Kelsi M. Knapp ◽  
Jackson H. Rogers ◽  
Courtney Berger ◽  
Elaine L. Shelton ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Comparative Analysis ◽  
Drug Discovery ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

The Effect of Dynamical Strain on the Maturation of Collagen-Based Cell-Containing Scaffolds for Vascular Tissue Engineering

2011 ◽  
Vol 409 ◽  
pp. 152-157 ◽  
Author(s):  
Lucie Levesque ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Collagen Fibrils ◽  
Vascular Tissue Engineering ◽  
Silicone Membrane ◽  
Custom Made

Diseases occurring to blood vessel are preferentially solved by replacing the vessel by an autologous graft. When it is not available, a synthetic graft is used which has low patency rates for small diameter (<6 mm) vessels. Tissue engineering of blood vessel aims to improve the performance of vascular substitutes. Bioreactors are used in vascular tissue engineering to mimic the mechanical and biochemical environment of blood vessel. A 2D bioreactor was custom made in order to impose a dynamical strain to silicone membrane receiving the collagen cell-based construct. Collagen gels with vascular smooth muscle cells cultured inside were subdued to maturation under dynamical uniaxial stretch regimes at 1Hz for 48 hours. The percentage of deformation encountered by the silicone membrane was measured by ImageJ. Collagen fibrils and porcine smooth muscle cells (PSMC) orientations were assessed by scanning electron microscopy (SEM). Results show that the study of mechanical conditioning on cell activity is an important issue for enhancing the alignment of collagen fibrils.

scholarly journals Urine-Derived Stem Cells: Differentiation Potential into Smooth-Muscle Cells and Urothelial Cell

2019 ◽  
Vol 74 (3) ◽  
pp. 176-184
Author(s):  
Igor A. Vasyutin ◽  
Aleksey V. Lyundup ◽  
Sergey L. Kuznetsov
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Urinary Tract ◽  
Urinary Bladder ◽  
Smooth Muscle Cells ◽  
Muscle Cells ◽  
Proliferative Potential ◽  
Differentiation Potential ◽  
Invasive Approach

Background: Tissue engineering of low urinary tract organs requires biopsy of urinary bladder material. The current study describes non-invasive approach of obtaining autologous stem cells from urine of healthy adults. These cells were studied for potential to differentiate into epithelial cells and smooth muscle cells of the urinary bladder. Aims: To describe properties of urine-derived stem cells (USCs) and investigate their differentiation potential for tissue engineering of low urinary tract organs. Materials and Methods: USCs were isolated from urine of healthy volunteers with centrifugation and seeded in media to 24-well plates. Expression of stem cells markers (CD73, CD90, CD105, CD34, CD45, CD29, CD44, CD54, SSEA4) by USCs was assessed with flow cytometry. Expression of specific markers of smooth muscle cells and urothelial cells was assessed with fluorescence microscopy with following computational image analysis. Results: Median number of USCs per 100 ml urine was 6. Doubling time for USC was 1.440.528 days (n=4) and there were 26.34.79 population doublings for USC cultures (n=4). Median expression of markers of postnatal stem cells was CD73 ― 79.8%, CD90 ― 56.6%, CD105 ― 40.7%, CD34 1.0%, CD45 2.0%, CD29 99.0%, CD44 99.0%, CD54 ― 97.7% and SSEA4 99.0%. Treatment of cells with high concentration of EGF in media with low concentration of FBS for 10 days increased cytokeratin (CK) expression to 24.9% for CK AE1/AE3 and to 7.6% for CK 7. Treatment of USCs with media inducing smooth muscle differentiation for 10 days increased expression of -smooth muscle actin to 79.6% and expression of calponin to 97.6%. Conclusions: USCs are cells that can be found in urine in small quantities. They have high proliferative potential and express markers of postnatal stem cells. Under effect of PDGF-BB and TGF- 1 they differentiate into smooth muscle cells.

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