The Effect of Passive Ultrasonic Activation of 2% Chlorhexidine or 5.25% Sodium Hypochlorite Irrigant on Residual Antimicrobial Activity in Root Canals

2003 ◽  
Vol 29 (9) ◽  
pp. 562-564 ◽  
Author(s):  
C WEBER ◽  
S MCCLANAHAN ◽  
G MILLER ◽  
M DIENERWEST ◽  
J JOHNSON
2014 ◽  
Vol 25 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Marcos Rodolfo Bolfoni ◽  
Marcelo dos Santos Ferla ◽  
Otávio da Silva Sposito ◽  
Luciano Giardino ◽  
Rogério de Castilho Jacinto ◽  
...  

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.


2017 ◽  
Vol 43 (7) ◽  
pp. 1161-1165 ◽  
Author(s):  
Roberto Estevez ◽  
Antonio J. Conde ◽  
Oliver Valencia de Pablo ◽  
Francisco de la Torre ◽  
Giampiero Rossi-Fedele ◽  
...  

2016 ◽  
Vol 50 (10) ◽  
pp. 976-982 ◽  
Author(s):  
A. J. Conde ◽  
R. Estevez ◽  
G. Loroño ◽  
Ó. Valencia de Pablo ◽  
G. Rossi-Fedele ◽  
...  

2009 ◽  
Vol 17 (6) ◽  
pp. 555-559 ◽  
Author(s):  
Marcia Carneiro Valera ◽  
Katy Costa Godinho da Silva ◽  
Lilian Eiko Maekawa ◽  
Cláudio Antonio Talge Carvalho ◽  
Cristiane Yumi Koga-Ito ◽  
...  

2019 ◽  
Vol 14 (1) ◽  
pp. 92
Author(s):  
Dr. Maha Abdul- Kareem Mahmood ◽  
Dr. Huda Elias Ali ◽  
Dr. Haraa Khairi Abdul-Kadher

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.


2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Giselle Nevares ◽  
Felipe Xavier ◽  
Luciana Gominho ◽  
Flávia Cavalcanti ◽  
Marcely Cassimiro ◽  
...  

This study aimed to analyse and compare apical extrusion of debris in canals instrumented with systems used in reciprocating and continuous motion. Sixty mandibular premolars were randomly divided into 3 groups (n=20): the Reciproc (REC), WaveOne (WO), and HyFlex CM (HYF) groups. One Eppendorf tube per tooth was weighed in advance on an analytical balance. The root canals were instrumented according to the manufacturer’s instructions, and standardised irrigation with 2.5% sodium hypochlorite was performed to a total volume of 9 mL. After instrumentation, the teeth were removed from the Eppendorf tubes and incubated at 37°C for 15 days to evaporate the liquid. The tubes were weighed again, and the difference between the initial and final weight was calculated to determine the weight of the debris. The data were statistically analysed using the Shapiro-Wilk, Wilcoxon, and Mann-Whitney tests (α=5%). All systems resulted in the apical extrusion of debris. Reciproc produced significantly more debris than WaveOne (p<0.05), and both systems produced a greater apical extrusion of debris than HyFlex CM (p<0.001). Cross section and motion influenced the results, despite tip standardization.


2015 ◽  
Vol 48 (12) ◽  
pp. 1188-1193 ◽  
Author(s):  
M. T. Arias-Moliz ◽  
R. Ordinola-Zapata ◽  
P. Baca ◽  
M. Ruiz-Linares ◽  
E. García García ◽  
...  

2014 ◽  
Vol 25 (6) ◽  
pp. 489-493 ◽  
Author(s):  
Marta Sáinz-Pardo ◽  
Roberto Estevez ◽  
Óliver Valencia de Pablo ◽  
Giampiero Rossi-Fedele ◽  
Rafael Cisneros

The purpose of this ex vivo study was to determine, in "open" and "closed" systems, whether the design has an influence on the penetration length of sodium hypochlorite mixed with a radiopaque contrast medium, measured in millimeters, when delivered using positive pressure (PP) and using sonic (SI) or passive ultrasonic (PUI) activation. Sixty single-rooted teeth were divided into two groups: open and closed systems (n=30). Root canal shaping was performed to a working length of 17 mm. The samples were divided into three sub-groups (n=10) according to irrigant delivery and activation: PP, and SI or PUI activation. By using radiographs, penetration length was measured, and vapor lock was assessed. For the closed group, the penetration distance means were: PP 15.715 (±0.898) mm, SI 16.299 (±0.738) mm and PUI 16.813 (±0.465) mm, with vapor lock occurring in 53.3% of the specimens. In the open group, penetration to 17 mm occurred in 97.6% of the samples, and no vapor lock occurred. Irrigant penetration and distribution evaluation using open and closed systems provide significantly different results. For closed systems, PUI is the most effective in delivering the irrigant to working length, followed by SI.


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