LC/ESI/MS analysis of the in-vivo metabolites of zotepine in the plasma of the patient and the in-vitro metabolites of zotepine produced in human microsome

This research investigated a UPLC-QTOF/ESI-MS-based phytochemical profiling of Combretum indicum leaf extract (CILEx), and explored its in vitro antioxidant and in vivo antidiabetic effects in a Long–Evans rat model. After a one-week intervention, the animals’ blood glucose, lipid profile, and pancreatic architectures were evaluated. UPLC-QTOF/ESI-MS fragmentation of CILEx and its eight docking-guided compounds were further dissected to evaluate their roles using bioinformatics-based network pharmacological tools. Results showed a very promising antioxidative effect of CILEx. Both doses of CILEx were found to significantly (p < 0.05) reduce blood glucose, low-density lipoprotein (LDL), and total cholesterol (TC), and increase high-density lipoprotein (HDL). Pancreatic tissue architectures were much improved compared to the diabetic control group. A computational approach revealed that schizonepetoside E, melianol, leucodelphinidin, and arbutin were highly suitable for further therapeutic assessment. Arbutin, in a Gene Ontology and PPI network study, evolved as the most prospective constituent for 203 target proteins of 48 KEGG pathways regulating immune modulation and insulin secretion to control diabetes. The fragmentation mechanisms of the compounds are consistent with the obtained effects for CILEx. Results show that the natural compounds from CILEx could exert potential antidiabetic effects through in vivo and computational study.

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GC-MS and LC-ESI-MS analysis of biologically active fractions from Verbascum letourneuxii; efficient protocol for in vitro propagation

Biocatalysis and Agricultural Biotechnology ◽

10.1016/j.bcab.2020.101817 ◽

2020 ◽

Vol 29 ◽

pp. 101817

Author(s):

Mai M. Farid ◽

Alia Y. Ragheb ◽

Mona El-Shabrawy ◽

Mona M. Marzouk ◽

Sameh R. Hussein ◽

...

Keyword(s):

In Vitro Propagation ◽

Biologically Active ◽

Esi Ms ◽

Ms Analysis

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iTRAQ-Based Quantitative Proteomic Comparison of 2D and 3D Adipocyte Cell Models Co-cultured with Macrophages Using Online 2D-nanoLC-ESI-MS/MS

Scientific Reports ◽

10.1038/s41598-019-53196-0 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Sun Young Lee ◽

Sung Bum Park ◽

Young Eun Kim ◽

Hee Min Yoo ◽

Jongki Hong ◽

...

Keyword(s):

Metabolic Disorders ◽

Cultured Cells ◽

Three Dimensional ◽

3D Cell Culture ◽

Proteomic Profiling ◽

Esi Ms ◽

Mitochondrial Fatty Acid ◽

2D And 3D

AbstractThe demand for novel three-dimensional (3D) cell culture models of adipose tissue has been increasing, and proteomic investigations are important for determining the underlying causes of obesity, type II diabetes, and metabolic disorders. In this study, we performed global quantitative proteomic profiling of three 3D-cultured 3T3-L1 cells (preadipocytes, adipocytes and co-cultured adipocytes with macrophages) and their 2D-cultured counterparts using 2D-nanoLC-ESI-MS/MS with iTRAQ labelling. A total of 2,885 shared proteins from six types of adipose cells were identified and quantified in four replicates. Among them, 48 proteins involved in carbohydrate metabolism (e.g., PDHα, MDH1/2, FH) and the mitochondrial fatty acid beta oxidation pathway (e.g., VLCAD, ACADM, ECHDC1, ALDH6A1) were relatively up-regulated in the 3D co-culture model compared to those in 2D and 3D mono-cultured cells. Conversely, 12 proteins implicated in cellular component organisation (e.g., ANXA1, ANXA2) and the cell cycle (e.g., MCM family proteins) were down-regulated. These quantitative assessments showed that the 3D co-culture system of adipocytes and macrophages led to the development of insulin resistance, thereby providing a promising in vitro obesity model that is more equivalent to the in vivo conditions with respect to the mechanisms underpinning metabolic syndromes and the effect of new medical treatments for metabolic disorders.

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In vivo and in vitro evaluation of hair growth potential of Cacumen Platycladi, and GC-MS analysis of the active constituents of volatile oil

Journal of Ethnopharmacology ◽

10.1016/j.jep.2019.111835 ◽

2019 ◽

Vol 238 ◽

pp. 111835

Author(s):

Yan Zhang ◽

Shanshan Chen ◽

Fanzhi Qu ◽

Guangyue Su ◽

Yuqing Zhao

Keyword(s):

Hair Growth ◽

Volatile Oil ◽

Growth Potential ◽

In Vitro Evaluation ◽

Active Constituents ◽

Ms Analysis

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Cerebral effect of optimized Allium sativum oil-loaded chitosan nanorods: GC-MS analysis and in vitro/in vivo evaluation

Food & Function ◽

10.1039/c9fo02911g ◽

2020 ◽

Vol 11 (6) ◽

pp. 5357-5376

Author(s):

Rabab Kamel ◽

Abeer Salama ◽

Nermine M. Shaffie ◽

Nesma M. Salah

Keyword(s):

Factorial Design ◽

Allium Sativum ◽

Biological Effects ◽

In Vivo Evaluation ◽

Promising Strategy ◽

Ms Analysis

Two-level orthogonal factorial design was used to optimize GO-loaded chitosan nanorods. This study proves that the nanoencapsulation of GO is a promising strategy which overcomes its limitations and improves its biological effects.

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IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i9.27879 ◽

2018 ◽

Vol 10 (9) ◽

pp. 68

Author(s):

Deep Chhavi Anand ◽

Rishikesh Meena ◽

Vidya Patni

Keyword(s):

Callus Induction ◽

Culture Media ◽

Biological Activities ◽

Gas Chromatography Mass Spectrometry ◽

Dodecanoic Acid ◽

Stem Explants ◽

Ms Analysis ◽

Wide Range

Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.

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