5. Effect of caffeine on the labeling of red blood cells and plasma proteins with technetium-99m

Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and perimeter/area ratio determination. P. flavicarpa extract altered (p<0.05) the fixation of 99mTc on plasma proteins and the perimeter/area ratio of red blood cells. Substances present in P. flavicarpa extract could affect the labeling of blood constituents with 99mTc acting in specific targets as membrane of red blood cells.

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Assessment of the effect of Maytenus ilicifolia (espinheira santa) extract on the labeling of red blood cells and plasma proteins with technetium-99m

Journal of Ethnopharmacology ◽

10.1016/s0378-8741(00)00240-3 ◽

2000 ◽

Vol 72 (1-2) ◽

pp. 179-184 ◽

Cited By ~ 29

Author(s):

Joelma Fonseca de Oliveira ◽

Ana Cristina S Braga ◽

Márcia B Nunes de Oliveira ◽

Antonio Sérgio Ávila ◽

Adriano Caldeira-de-Araújo ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Plasma Proteins ◽

Technetium 99M ◽

Maytenus Ilicifolia

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Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600022 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 175-182 ◽

Cited By ~ 6

Author(s):

Severo de Paoli ◽

Tania Santos Giani ◽

Giuseppe Antonio Presta ◽

Marcia Oliveira Pereira ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Cellular Membrane ◽

Area Ratio ◽

Morphometric Parameter ◽

Blood Constituents ◽

Technetium 99M ◽

Clinical Procedures ◽

Clove Extract ◽

Blood Fraction

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the morphology of red blood cells by oxidative/chelating actions or interacting with the cellular membrane structure.

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