scholarly journals Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 153-159 ◽  
Author(s):  
Bernardo Machado Rebello ◽  
Silvana Ramos Farias Moreno ◽  
Camila Godinho Ribeiro ◽  
Rosane de Figueiredo Neves ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Area Ratio ◽  
Passiflora Edulis ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Morphological Evaluation ◽  
Blood Smears ◽  
Ratio Determination

Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and perimeter/area ratio determination. P. flavicarpa extract altered (p<0.05) the fixation of 99mTc on plasma proteins and the perimeter/area ratio of red blood cells. Substances present in P. flavicarpa extract could affect the labeling of blood constituents with 99mTc acting in specific targets as membrane of red blood cells.

scholarly journals Effects of clove (Caryophyllus aromaticus L.) on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 175-182 ◽  
Author(s):  
Severo de Paoli ◽  
Tania Santos Giani ◽  
Giuseppe Antonio Presta ◽  
Marcia Oliveira Pereira ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Cellular Membrane ◽  
Area Ratio ◽  
Morphometric Parameter ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Clinical Procedures ◽  
Clove Extract ◽  
Blood Fraction

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the morphology of red blood cells by oxidative/chelating actions or interacting with the cellular membrane structure.

scholarly journals Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 145-152 ◽  
Author(s):  
Giuseppe Antonio Presta ◽  
Sebastião David Santos-Filho ◽  
Severo de Paoli ◽  
Tania Santos Giani ◽  
Adalgisa Ieda Maiworm ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Area Ratio ◽  
Membrane Structures ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Clinical Disorders ◽  
Treatment Of Diabetes

Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P<0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of blood constituents with 99mTc by its chelating/antioxidant action and/or effects on membrane structures involved in the ion transport.

scholarly journals Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

2007 ◽  
Vol 50 (spe) ◽  
pp. 167-174 ◽  
Author(s):  
Rosane de Figueiredo Neves ◽  
Silvana Ramos Farias Moreno ◽  
Bernardo Machado Rebello ◽  
Luiz Querino de Araújo Caldas ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Nuclear Medicine ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Area Ratio ◽  
Arctium Lappa ◽  
Blood Samples ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Inflammatory Processes

Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the %ATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.

scholarly journals Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats

2008 ◽  
Vol 51 (spe) ◽  
pp. 127-133 ◽  
Author(s):  
Gabrielle de Souza Rocha ◽  
Marcia de Oliveira Pereira ◽  
Mônica de Oliveira Benarroz ◽  
Jacques Natan Grinapel Frydman ◽  
Angélica Beatriz Garcia-Pinto ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Quantitative Morphology ◽  
Blood Samples ◽  
Blood Constituents ◽  
Qualitative And Quantitative ◽  
Technetium 99M ◽  
Sodium Pertechnetate ◽  
Blood Smears

This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical microscopy. In biodistribution experiments, sodium pertechnetate was administrated, organs and tissues isolated, radioactivity was counted and percentage of incorporated radioactivity per gram (%ATI/g) determined. The data showed no significant alterations in %ATI, morphology of RBC and in %ATI/g in the studied organs.

scholarly journals Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid

2008 ◽  
Vol 51 (spe) ◽  
pp. 135-141 ◽  
Author(s):  
Marcia de Oliveira Pereira ◽  
Gabrielle de Souza Rocha ◽  
Simone dos Santos Lombardi ◽  
Mauro Geller ◽  
Mário José Pereira ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Stannous Chloride ◽  
Quantitative Morphology ◽  
Blood Constituents ◽  
Qualitative And Quantitative ◽  
Technetium 99M ◽  
Rbc Membrane ◽  
Blood Smears ◽  
Chloride Effect

The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel electrophoresis procedure was carried out to evaluate genotoxic and the protection of this drug against stannous chloride effect on DNA. In conclusion, under the conditions used in this work, our data suggest that fenoprofen would not (i) affect the fixation of the 99mTc on the blood constituents, (ii) alter the RBC membrane and (iii) present genotoxic and redox effects.

Effect of ginkgo biloba on the in vitro labeling of red blood cells and plasma proteins with technetium-99M

2001 ◽  
Vol 44 (S1) ◽  
pp. S639-S641 ◽  
Author(s):  
S. R. F. Moreno ◽  
G. D. Feliciano ◽  
R. S. Freitas ◽  
D. M. Mattos ◽  
M. L. Gomes ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Ginkgo Biloba ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Technetium 99M

Zinc-oxide-eugenol alters labeling of blood constituents with technetium-99m and shape of red blood cells

2008 ◽  
Vol 18 (5) ◽  
pp. 373-382
Author(s):  
Severo de Paoli ◽  
Tania Santos Giani ◽  
Giuseppe Antonio Presta ◽  
Carlos Guilherme Correa ◽  
Adalgisa Ieda Maiworm ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Blood Constituents ◽  
Technetium 99M ◽  
Zinc Oxide Eugenol

scholarly journals Effects of Cinnamomum zeylanicum treatment on radiolabeling of blood bonstituents and morphology of red blood cells in Wistar rats

2008 ◽  
Vol 51 (spe) ◽  
pp. 143-149 ◽  
Author(s):  
Mônica Oliveira Benarroz ◽  
Gabrielle de Souza Rocha ◽  
Márcia Oliveira Pereira ◽  
Mauro Geller ◽  
Adenilson de Souza da Fonseca ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Wistar Rats ◽  
Membrane Structures ◽  
Blood Constituents ◽  
Cinnamon Extract ◽  
Blood Smears ◽  
Transport Of Ions ◽  
Different Doses

The aim of this study was to evaluate the effect of in vivo treatment with an aqueous cinnamon extract on the labeling of blood constituents with 99mTc and on the morphology of red blood cells from Wistar rats. Animals were treated with cinnamon extract at different doses and for different periods of time. As controls, animals treated with 0.9% NaCl. Labeling of blood constituents with 99mTc was performed. Plasma, blood cells and insoluble fractions were isolated. Radioactivity in each fraction was counted and the percentage of radioactivity (%ATI) was calculated. Also, blood smears were prepared to morphological analysis of red blood cells from. Data showed that in vivo cinnamon extract did not significantly (p>0.05) modify the %ATI of blood constituents and morphology of red blood cells. The results suggest that in vivo aqueous cinnamon could not affect the membrane structures involved in transport of ions or the oxidation state of stannous and pertechnetate ions.

5. Effect of caffeine on the labeling of red blood cells and plasma proteins with technetium-99m

1999 ◽  
Vol 20 (10) ◽  
pp. 954-955
Author(s):  
S. R.F. Moreno ◽  
A. P. Motta ◽  
R. C. Cardoso ◽  
G. B.X. Lins ◽  
M. Bernardo-Filho
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Plasma Proteins ◽  
Technetium 99M
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