Zinc-oxide-eugenol alters labeling of blood constituents with technetium-99m and shape of red blood cells

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this work was to evaluate the effects of clove extract on the labeling blood constituents with 99mTc and on the morphology of red blood cells. Blood samples were incubated with clove, stannous chloride and 99mTc. Plasma, blood cells, insoluble fractions of plasma and blood cells were separated. The radioactivity was counted and percentage of radioactivity (%ATI) to each blood fraction was calculated. The shape and morphometric parameter (perimeter/area ratio) were evaluated. Clove extract altered significantly (p<0.05) the %ATI of blood constituents and the shape of red blood cells without modifying the perimeter/area ratio. The results indicate that clove extract presents chemical compounds that interfere with the radiolabeling of blood constituents and alter the morphology of red blood cells by oxidative/chelating actions or interacting with the cellular membrane structure.

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Evaluation of biologic effects of an Ilex paraguariensis aqueous extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp12.998 ◽

2013 ◽

Vol 7 (40) ◽

pp. 2686-2692 ◽

Cited By ~ 1

Author(s):

S. Braga

Keyword(s):

Red Blood Cells ◽

Aqueous Extract ◽

Blood Cells ◽

Ilex Paraguariensis ◽

Blood Constituents ◽

Technetium 99M ◽

Biologic Effects

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Experimental models using radionuclides for assessment of the effects of natural drugs

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000700027 ◽

2008 ◽

Vol 51 (spe) ◽

pp. 169-174 ◽

Cited By ~ 2

Author(s):

Silvana Ramos Farias Moreno ◽

Jorge José de Carvalho ◽

Bernardo Rebello ◽

Emely Kazan Rocha ◽

Margareth de Oliveira Timóteo ◽

...

Keyword(s):

Red Blood Cells ◽

Ginkgo Biloba ◽

Blood Cells ◽

Experimental Models ◽

Significant Alteration ◽

Blood Constituents ◽

Technetium 99M ◽

Sodium Pertechnetate ◽

Natural Drugs

The aim of this study was to evaluate the effect of the extracts of Nectandra membranacea (N. membranacea), Ginkgo biloba (EGb) and Passiflora (PEF) on the morphology of red blood cells (RBC), on the biodistribution of sodium pertechnetate (99mTcO4Na), on the morphology of duodenum and on the labeling of blood constituents (BC, IF-P, IF-BC) with technetium-99m (Tc-99m). Morphometry studies also were performed. The results show that EGb promotes alteration of the labeling of BC, IF-P and IF-BC (p<0.05). The N. membranacea extract does not promote significant alteration of the radiolabeling, and PEF extract alters the IF-P labeling. N. membranacea, EGb and PEF extracts were able to alter the RBC morphology (P<0.05). N. membranacea extract and EGb modifies the biodistribution of the 99mTcO4Na, and EGb influences the morphometry of duodenum isolated from rats (P<0.05).

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Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600019 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 153-159 ◽

Cited By ~ 4

Author(s):

Bernardo Machado Rebello ◽

Silvana Ramos Farias Moreno ◽

Camila Godinho Ribeiro ◽

Rosane de Figueiredo Neves ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Plasma Proteins ◽

Area Ratio ◽

Passiflora Edulis ◽

Blood Constituents ◽

Technetium 99M ◽

Morphological Evaluation ◽

Blood Smears ◽

Ratio Determination

Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and perimeter/area ratio determination. P. flavicarpa extract altered (p<0.05) the fixation of 99mTc on plasma proteins and the perimeter/area ratio of red blood cells. Substances present in P. flavicarpa extract could affect the labeling of blood constituents with 99mTc acting in specific targets as membrane of red blood cells.

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Effects of Chrysobalanus icaco on the labeling of blood constituents with technetium-99m and on the shape of the red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600018 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 145-152 ◽

Cited By ~ 3

Author(s):

Giuseppe Antonio Presta ◽

Sebastião David Santos-Filho ◽

Severo de Paoli ◽

Tania Santos Giani ◽

Adalgisa Ieda Maiworm ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Area Ratio ◽

Membrane Structures ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M ◽

Clinical Disorders ◽

Treatment Of Diabetes

Chrysobalanus icaco (abajeru; C.icaco) is recommended in the treatment of diabetes and other clinical disorders. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The aim of this study was to verify the effects of an abajeru extract on the labeling of blood constituents with 99mTc and on the shape of red blood cells (RBC). Blood samples(Wistar rats) were incubated with abajeru extract and the labeling of blood constituents with 99mTc and morphology of RBC were carried out. The results showed significant (P<0.05) alteration of labeling of blood constituents with 99mTc and the morphometry (perimeter/area ratio) of the RBC in presence of the extract. These data suggest that this abajeru extract could alter the labeling of blood constituents with 99mTc by its chelating/antioxidant action and/or effects on membrane structures involved in the ion transport.

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Effect of an Arctium lappa (burdock) extract on the labeling of blood constituents with technetium-99m and on the morphology of the red blood cells

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132007000600021 ◽

2007 ◽

Vol 50 (spe) ◽

pp. 167-174 ◽

Cited By ~ 2

Author(s):

Rosane de Figueiredo Neves ◽

Silvana Ramos Farias Moreno ◽

Bernardo Machado Rebello ◽

Luiz Querino de Araújo Caldas ◽

Adenilson de Souza da Fonseca ◽

...

Keyword(s):

Nuclear Medicine ◽

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Area Ratio ◽

Arctium Lappa ◽

Blood Samples ◽

Blood Constituents ◽

Technetium 99M ◽

Inflammatory Processes

Arctium lappa (burdock) has been used to treat inflammatory processes. Blood constituents labeled with technetium-99m (99mTc) have been utilized in nuclear medicine. It was evaluated the influence of a burdock extract on the labeling of blood constituents with 99mTc and on the morphometry of red blood cells. Blood samples from Wistar rats were incubated with burdock extract and the radiolabeling procedure was carried out. Plasma and blood cells, soluble and insoluble fractions of plasma and blood cells were separated. The radioactivity in each fraction was counted and the percentages of radioactivity (%ATI) were determined. Morphology and morphometric (perimeter/area ratio) measurements of red blood cells (RBC) were performed. The incubation with burdock extract significantly (p<0.05) altered the %ATI on the blood compartments and the perimeter/area ratio of RBC, as well as, induced modifications on the shape of RBC. Alterations on membrane could justify the decrease of labeling of blood cells with 99mTc obtained in this study.

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Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132008000700021 ◽

2008 ◽

Vol 51 (spe) ◽

pp. 127-133 ◽

Cited By ~ 2

Author(s):

Gabrielle de Souza Rocha ◽

Marcia de Oliveira Pereira ◽

Mônica de Oliveira Benarroz ◽

Jacques Natan Grinapel Frydman ◽

Angélica Beatriz Garcia-Pinto ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Wistar Rats ◽

Quantitative Morphology ◽

Blood Samples ◽

Blood Constituents ◽

Qualitative And Quantitative ◽

Technetium 99M ◽

Sodium Pertechnetate ◽

Blood Smears

This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical microscopy. In biodistribution experiments, sodium pertechnetate was administrated, organs and tissues isolated, radioactivity was counted and percentage of incorporated radioactivity per gram (%ATI/g) determined. The data showed no significant alterations in %ATI, morphology of RBC and in %ATI/g in the studied organs.

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