Anti-V3 and Anti-IgG Antibodies of Healthy Individuals Share Complementarity Structures

1999 ◽  
Vol 21 (4) ◽  
pp. 266 ◽  
Author(s):  
R. Metlas ◽  
D. Trajkovic ◽  
T. Srdic ◽  
V. Veljkovic ◽  
A. Colombatti
Keyword(s):  
Igg Antibodies ◽  
Healthy Individuals

Prevalence of Toxoplasma gondii and Toxocara canis among Myositis Patients in Southwest of Iran

2019 ◽  
Vol 20 ◽  
Author(s):  
Jasem Saki ◽  
Karim Mowla ◽  
Reza Arjmand ◽  
Forough Kazemi ◽  
Somayeh Fallahizadeh
Keyword(s):  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Healthy Subjects ◽  
Toxocara Canis ◽  
Control Group ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
High Prevalence ◽  
Acute Toxoplasmosis ◽  
Southwest Of Iran

Introduction: Parasitic myositis is caused by some parasites such as T. gondii and T. canis. So, the aim of the study was to evaluate the prevalence T. gondii and T. canis in patients with myositis and healthy individuals. Methods: A total of 108 samples were randomly selected as the control (54 healthy individuals) and test (54 myositis patients) groups. IgG and IgM antibodies against T. gondii and IgG antibodies against T. canis were measured by the ELISA. The detection of chronic and acute toxoplasmosis was performed by the ELISA IgG avidity. The presence of T. gondii in blood was evaluated by the nested-PCR. Results: Of 108, 33 (30.6%) cases were detected positive for IgG against T. gondii that 19 (35.2%) and 14 (25.9%) were observed in myositis patients and healthy individuals, respectively (P=0.296). Of 19 positive cases, 12 (63.2%) and 7 (36.8%) cases were detected as chronic and acute toxoplasmosis, respectively, while, all positive cases in the control group had chronic toxoplasmosis (P=0.013). One (1.9%) sample was detected positive for anti- Toxoplasma gondii IgM and two (3.7%) samples were found positive for IgG against T. canis by the ELISA that these positive cases were observed only in myositis patients (P=1.000 P=0.495, respectively). B1 T. gondii gene was amplified in 12 (63.2%) and 1 (7.1%) in myositis patients and healthy subjects (P=0.001). Conclusions: Our findings showed that there was a relatively high prevalence of acute toxoplasmosis in myositis patients in comparison with the control subjects in southwest of Iran.

scholarly journals Immunoglobulin G Antibodies against the Endosymbionts of Filarial Nematodes (Wolbachia) in Patients with Pulmonary Dirofilariasis

2003 ◽  
Vol 10 (1) ◽  
pp. 180-181 ◽  
Author(s):  
F. Simón ◽  
G. Prieto ◽  
R. Morchón ◽  
C. Bazzocchi ◽  
C. Bandi ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Dirofilaria Immitis ◽  
Surface Protein ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Bacterial Endosymbiont ◽  
Filarial Nematodes ◽  
Pulmonary Dirofilariasis ◽  
Diagnostic Applications

ABSTRACT The dog parasite Dirofilaria immitis can infect humans. Patients with pulmonary dirofilariasis were tested for immunoglobulin G (IgG) antibodies against the surface protein of Wolbachia, the bacterial endosymbiont of D. immitis. These patients showed significantly higher IgG titers than healthy individuals from areas in which D. immitis was endemic as well as areas in which it was not endemic. Titration of anti-Wolbachia surface protein IgG could become useful for diagnostic applications.

scholarly journals Detection of antibodies to recombinant truncated flagellin and sonicated whole cell antigen of Burkholderia pseudomallei in acute melioidosis and in healthy Bangladeshi individuals

2020 ◽  
Vol 14 (1) ◽  
pp. 47-52
Author(s):  
Md Shariful Alam Jilani ◽  
Tang Thean Hock ◽  
Sraboni Mazumder ◽  
Fahmida Rahman ◽  
Md Mohiuddin ◽  
...  
Keyword(s):  
Rural Areas ◽  
Burkholderia Pseudomallei ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Serum Samples ◽  
Igg Antibody ◽  
Whole Cell ◽  
Cell Antigen ◽  
The Mean

Background and objectives: Several types of Burkholderia pseudomallei antigens have been used to determine the antibody response in acute and asymptomatic cases. In the present study, we have detected immunoglobulin G (IgG) antibody to recombinant truncated flagellin antigen (RTFA) of B. pseudomallei in the sera of acute melioidosis cases and healthy individuals from melioidosis endemic areas of Bangladesh by indirect enzyme-linked immunosorbent assay (ELISA). In parallel, IgG antibody to sonicated whole cell antigen (SWCA) of B. pseudomallei was determined to compare with anti-RTFA antibody. Methodology: Serum samples from culture confirmed melioidosis cases and from healthy individuals aged 21 years and above residing in melioidosis endemic rural areas were included in the study. Serum IgG antibody to RTFA and SWCA of B. pseudomallei was determined by indirect ELISA. Results: Out of 8 culture confirmed acute melioidosis cases, 7 (87.5%) and 8 (100%) were positive for anti-B. pseudomallei IgG antibodies by RTFA and SWCA methods respectively. Among 361 healthy individuals, the rate of seropositivity by RTFA-ELISA was significantly less than that of SWCA-ELISA (16.1% versus 26.8%; p = 0.001). The mean optical density (OD) of RTFA-ELISA of positive cases was significantly less than that of SWCA-ELISA in both melioidosis and healthy individuals (0.79±0.11 versus 2.4±0.08, p = 0.0001; 0.67±0.01 versus 1.27±0.02, p = 0.0001). The sensitivity and specificity of RTFA-ELISA were 88.9% and 100% respectively. Conclusion: Findings of the study suggest that multiple or combination of antigens should be used to study the seroprevalence of B. pseudomallei infection in a community. Also, prospective study is necessary to find out the duration of persistence of antibodies to different antigenic components of B. pseudomallei after exposure. Ibrahim Med. Coll. J. 2020; 14(1): 47-52

scholarly journals Colorectal Carcinoma Affected Patients Are Significantly Poor Responders Against the Oncogenic JC Polyomavirus

2021 ◽  
Vol 12 ◽  
Author(s):  
Elena Torreggiani ◽  
Ilaria Bononi ◽  
Silvia Pietrobon ◽  
Elisa Mazzoni ◽  
Giovanni Guerra ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Synthetic Peptides ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poor Responders ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Jc Polyomavirus ◽  
Oncogenic Potential ◽  
Different Types ◽  
Immunological Dysfunction

BackgroundMany investigations reported the association between human tumors and JCPyV, a polyomavirus with oncogenic potential. The association has been supported by studies that found JCPyV footprints in CRC and gliomas of different types. Indeed, JCPyV footprints including its nucleic acids and Tag oncoprotein have been revealed in CRC tissues.MethodsHerein, sera from colorectal carcinoma (CRC) affected patients and healthy individuals (HS), employed as control, were analysed for immunoglobulin G (IgG) antibodies against specific JCPyV viral capsid protein 1 (VP1) antigens. The investigation was carried out employing an innovative immunological assay. Indeed, an indirect enzyme-linked immunosorbent assay (ELISA) with JCPyV VP1 mimotopes was used. JCPyV VP1 mimotopes consisted of synthetic peptides mimicking VP1 epitopes.ResultsSera from CRC affected patients, evaluated using indirect ELISAs with synthetic mimotopes, showed a significant lower prevalence of IgG antibodies against JCPyV VP1 mimotopes (26%) compared to HS (51%), p<0.005. These data were confirmed by another method, the hemagglutination inhibition (HAI) assay. Altogether these results, i.e. the prevalence of serum IgG antibodies against JCPyV VP1 mimotopes from patients with CRC is approximately 50% lower than in HS, are of interest.DiscussionOur data suggest that patients with CRC are significantly poor responders against JCPyV VP1 antigens. It is possible that CRC patients are affected by a specific immunological deregulation. This immunological dysfunction, revelled in CRC patients, may account for their predisposition to the colorectal carcinoma onset.

scholarly journals Identification, Molecular Cloning, and Evaluation of Potential Use of Isocitrate Dehydrogenase II of Mycobacterium bovis BCG in Serodiagnosis of Tuberculosis

2002 ◽  
Vol 9 (4) ◽  
pp. 846-851 ◽  
Author(s):  
W. Florio ◽  
D. Bottai ◽  
G. Batoni ◽  
S. Esin ◽  
M. Pardini ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Isocitrate Dehydrogenase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Peptide Mass Fingerprinting ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Recombinant Gene Expression ◽  
Serum Dilution ◽  
Peptide Mass ◽  
Potential Use

ABSTRACT Diagnosis of tuberculosis is time-consuming and requires infrastructures which are often not available in countries with high incidences of the disease. In the present study, an 82-kDa protein antigen was isolated by affinity chromatography and was identified by peptide mass fingerprinting as isocitrate dehydrogenase II, which is encoded by the icd2 gene of Mycobacterium bovis BCG. The icd2 gene of BCG was cloned by PCR, and the product of recombinant gene expression was purified and analyzed by two-dimensional polyacrylamide gel electrophoresis. The recombinant protein, named rICD2, was tested for its recognition by immunoglobulin G (IgG) antibodies from the sera of 16 patients with tuberculosis (TB) and 23 healthy individuals by Western blotting. The results showed that rICD2 is recognized by IgG antibodies from the sera of all TB patients tested at serum dilutions of ≥1:640. At a serum dilution of 1:1,280, the sensitivity was 50% and the specificity was 86.9%. These results indicate that rICD2 might represent a candidate for use in a new assay for the serodiagnosis of TB.

scholarly journals Amyloid-Beta 1-42 Cross-Reactive Antibody Prevalent in Human Sera May Contribute to Intraneuronal Deposition of A-Beta-P-42

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Aristo Vojdani ◽  
Elroy Vojdani
Keyword(s):  
Cross Reactivity ◽  
Immune Reactivity ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Barrier Dysfunction ◽  
Autoreactive Antibodies ◽  
Human Sera ◽  
The Mean ◽  
The Brain ◽  
Reactive Antibody

Antibodies against many neural antigens are detected in the sera of both patients with Alzheimer’s disease (AD) and some healthy individuals. Blood-brain barrier dysfunction could make it possible for brain-reactive autoantibodies to reach the brain, where they can react with amyloid ß peptide (AßP). The origin of these autoreactive antibodies in the blood is unclear. The goals of this study were as follows: (1) to examine the immune reactivity of anti-AßP-42 with 22 neuronal and other associated antigens, some of which are involved in the pathophysiology of AD; (2) to classify antibodies to these 22 different antigens into those that cross-react with AßP-42 and those that do not; (3) to determine whether these antibodies react with BBB proteins, nerve growth factors, and enteric neuronal antigens. Using monoclonal AßP-42 antibody and ELISA methodology, we found that the antibody was highly reactive with Aß protein, tau protein, presenilin, rabaptin-5,β-NGF, BDNF, mTG, and enteric nerve. The same antibody produced equivocal to moderate reactions with glutamate-R, S100B, AQP4, GFAP, MBP,α-synuclein, tTG-2, and tTG-3, and not with the rest. These antibodies were also measured in blood samples from 47 AD patients and 47 controls. IgG antibodies were found to be elevated against AßP-42 and many other antigens in a significant percentage of controls. Overall, the mean OD values were significantly higher against 9/23 tested antigens (p<0.001) in the samples with AD. We were indeed able to classify the detected neuronal antibodies into those that cross-react with AßP-42 and those that do not. Our main finding is that although these antibodies may be harmless in a subgroup of controls, in individuals with compromised BBBs these antibodies that cross-react with AßP-42 can reach the brain, where their cross-reactivity with AßP-42 may contribute to the onset and progression of AD, and perhaps other neurodegenerative disorders.

scholarly journals Evaluation of enzyme-linked immunosorbent assay [ELISA]in serodiagnosis of pulmonary tuberculosis

1997 ◽  
Vol 3 (3) ◽  
pp. 480-492
Author(s):  
M. G. Morsi
Keyword(s):  
Pulmonary Tuberculosis ◽  
Immunosorbent Assay ◽  
Microscopic Examination ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sputum Smear ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Parallel Application ◽  
Positive Diagnosis ◽  
Chest Examination

A total of 45 patients with pulmonary tuberculosis and 45 healthy individuals were subjected to chest examination, radiography and ELISA tests for IgA and IgG antibodies. Sputum smear and culture were performed for all tuberculous patients. Evaluated against clinical and radiological diagnosis, ELISA’s specificity exceeded 90% in detecting specific IgA and IgG antibodies. The parallel application of ELISA and microscopic examination of sputum yielded 80% sensitivity compared with clinical and radiological examination and 100% sensitivity compared with culture. ELISA alone can be used in ruling out pulmonary tuberculosis but not in diagnosing the disease. However, coupled with microscopic examination, it can be used instead of culture to provide positive diagnosis within 24 hours

scholarly journals What is аsthma-COPD Overlap syndrome (ACOS), diagnostics and treatment

2018 ◽  
Vol 15 (2) ◽  
pp. 17-28
Author(s):  
G B Fedoseev ◽  
V I Trofimov ◽  
K V Negrutsa ◽  
V G Timchik ◽  
V I Golubeva ◽  
...  
Keyword(s):  
Respiratory Function ◽  
Overlap Syndrome ◽  
Negative Association ◽  
Chronic Obstructive ◽  
Igg Antibodies ◽  
Healthy Individuals ◽  
Laboratory Examination ◽  
Obstructive Pulmonary Disease ◽  
Function Test ◽  
Bacterial Antigens

175 patients were examined: 78 patients with bronchial asthma (BA), 39 with Asthma-COPD Overlap Syndrome (ACOS), 38 with chronic obstructive pulmonary disease (COPD) and 20 healthy individuals. A general clinical and laboratory examination was conducted, α1-antitrypsin (AAT), IgG and IgA to four bacterial antigens were determined, and pulmonary function test was studied. The clinical and spirometry data (characterizing the ACOS) were received. Negative association of AAT with respiratory function parameters in patients with ACOS, high levels of IgG antibodies to bacterial antigens and the lack of these connections in patients with BA and COPD allows us to consider ACOS to be independent nosological form related with microbial inflammation and marked by the AAT.

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