Vascular endothelial growth factor promotes physical wound repair and is anti-apoptotic in primary distal lung epithelial and A549 cells

2007 ◽  
Vol 35 (9) ◽  
pp. 2164-2170 ◽  
Author(s):  
Jonathan R. Roberts ◽  
Gavin D. Perkins ◽  
Takeshi Fujisawa ◽  
Kerry A. Pettigrew ◽  
Fang Gao ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Wound Repair ◽  
A549 Cells ◽  
Vascular Endothelial ◽  
Lung Epithelial ◽  
Distal Lung ◽  
Endothelial Growth Factor

scholarly journals Plasmin Modulates Vascular Endothelial Growth Factor-A-Mediated Angiogenesis during Wound Repair

2006 ◽  
Vol 168 (2) ◽  
pp. 670-684 ◽  
Author(s):  
Detlev Roth ◽  
Michael Piekarek ◽  
Mats Paulsson ◽  
Hildegard Christ ◽  
Wilhelm Bloch ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Wound Repair ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Expression and regulation of vascular endothelial growth factor in human pulmonary epithelial cells

2000 ◽  
Vol 279 (2) ◽  
pp. L371-L378 ◽  
Author(s):  
Sandrine Boussat ◽  
Saadia Eddahibi ◽  
André Coste ◽  
Virginie Fataccioli ◽  
Mallaury Gouge ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Epithelial Cells ◽  
Lung Epithelial Cells ◽  
Vascular Endothelial ◽  
Vegf Expression ◽  
Bronchial Epithelial ◽  
Lung Epithelial ◽  
Endothelial Growth Factor

Vascular endothelial growth factor (VEGF) is a potent endothelial cell growth and permeability factor highly expressed in rodent alveolar epithelium after injury and repair. To investigate VEGF synthesis in human lung epithelial cells, we examined VEGF expression by cultured cells under basal conditions and after cytokine treatment or oxidative stress. Basal VEGF expression was detected in transformed human epithelial cell lines (A549 and 1HAEo−) and in primary human bronchial epithelial cells with RT-PCR, Western blot, and immunocytochemistry. Among the cytokines tested, only transforming growth factor-β1 increased the levels of excreted VEGF165 as measured by ELISA. Under hypoxia (0% O2 for 24 h), the VEGF165 level increased fivefold, and this effect was O2 concentration dependent. VEGF concentrations in the medium of all the cell types studied reached values similar to those found in bronchoalveolar lavage fluids from normal patients. Endothelial cells (human umbilical vein endothelial cells) exposed to conditioned medium from primary bronchial epithelial cell cultures showed an increased growth rate, which was inhibited in the presence of a specific neutralizing antibody to VEGF. These results suggest that lung epithelial cells participate in the endothelial repair and angiogenesis that follow lung injury through the synthesis of VEGF.

Plasma Exosomes Loaded pH-Responsive Carboxymethylcellulose Hydrogel Promotes Wound Repair by Activating the Vascular Endothelial Growth Factor Signaling Pathway in Type 1 Diabetic Mice

2021 ◽  
Vol 17 (10) ◽  
pp. 2021-2033
Author(s):  
Lijuan Huang ◽  
Yijie Shi ◽  
Mengdie Li ◽  
Tao Wang ◽  
Liang Zhao
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Wound Healing ◽  
Growth Factor ◽  
Wound Repair ◽  
Vascular Endothelial ◽  
Diabetic Wound ◽  
Ph Responsive ◽  
Diabetic Wound Healing ◽  
Endothelial Growth Factor

Chronic wound healing plagues thousands of diabetic patients and brings social and economic burdens. Plasma exosomes (P-Exos), regarded as nanosized therapeutic agents, have shown therapeutic efficacy in promoting diabetic wound healing. The present work prepared the P-Exos-loaded pH-responsive carboxymethylcellulose (P-Exos-loaded CMC) hydrogel to investigate its ability to accelerate diabetic wound healing and to explore its underlying mechanisms. The results showed that the P-Exos-loaded CMC hydrogel was an effective therapeutic agent for accelerating diabetic wound repair. It promoted the local wound healing process in diabetic type 1 mice and enhanced angiogenesis and re-epithelialization via activating angiogenesis-related pathways mediated by vascular endothelial growth factor (VEGF).

An Experimental Study on the Regulation of Vascular Endothelial Growth Factor and Its Receptor in Rat Wound Tissue by Zibai Ointment

2020 ◽  
Vol 10 (7) ◽  
pp. 999-1004
Author(s):  
Er-Wei Cai ◽  
Cheng Zhao ◽  
Yan-Yan Wu ◽  
Wei-Juan Wang ◽  
Zhen-Peng Xu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Wound Repair ◽  
Vascular Endothelial ◽  
Collagen Deposition ◽  
Rt Pcr ◽  
Endothelial Growth Factor ◽  
Scanning Electron ◽  
Wound Tissue

Vascular endothelial growth factor (VEGF) and its receptor (VEGFR2) are important for wound repair. The aim of this study was to investigate the effect of Zibai ointment on the expression of these factors in wound models. In order to establish wound models, a total of 120 rats were used, which were assigned to vaseline group, Zibai ointment group and erythromycin group. The granulation tissues in each group were obtained on the 3rd, 7th, 14th or 21st day after the establishment of the model. The methods such as immunohistochemistry, RT-PCR, and western blot were used to detect the expression of VEGF and VEGFR2. Comparing to the erythromycin group or vaseline group, the expression of VEGF and VEGFR2 in Zibai ointment group on the 21st day was much higher (P < 0 05). The results of scanning electron microscopy (SEM) showed that with time in the Zibai ointment group, the granulation tissue gradually formed dense reticular layers of loose, curly fibers, and presented with more collagen deposition. In conclusion, Zibai ointment could effectively improve the expression of VEGF and VEGFR2 in granulation tissues of wounds and promote wound healing.

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