Effect of streptozotocin-induced diabetes mellitus on the cerebellar cortex of adult male albino rats

Currently, cardiovascular diseases are the leading cause of global mortality, while diabetes mellitus remains an important cause of cardiovascular morbidity. A recent study showed that patients with diabetes mellitus treated with mineralocorticoid receptor antagonists have improved coronary microvascular function, leading to improved diastolic dysfunction. In this study, we evaluated the influence of acute administration of spironolactone on myocardial function in rats with streptozotocin-induced diabetes mellitus, with special emphasis on cardiodynamic parameters in diabetic rat hearts. The present study was carried out on 40 adult male Wistar albino rats (8 weeks old). Rats were randomly divided into 4 groups (10 animals per group): healthy rats treated with 0.1 μmol/L of spironolactone, diabetic rats treated with 0.1 μmol/L of spironolactone, healthy rats treated with 3 μmol/L of spironolactone, and diabetic rats treated with 3 μmol/L of spironolactone. Different, dose-dependent, acute responses of spironolactone treatment on isolated, working diabetic and healthy rat heart were observed in our study. In healthy rats, better systolic function was achieved with higher spironolactone dose, while in diabetic rats, similar effects of low and high spironolactone dose were observed.

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The Effect of Diet Coke and Monosodium Glutamate on the Cerebellar Cortex of Adult Male Albino Rats. Histological and Immuno-histochemical Study

Egyptian Journal of Histology ◽

10.21608/ejh.2019.7279.1071 ◽

2019 ◽

Vol 42 (2) ◽

pp. 437-452 ◽

Cited By ~ 1

Author(s):

Noha Abd El-Wahed ◽

Esmat Geith ◽

Nagwa Kalleny ◽

Hadwa Abd Al-Khalek

Keyword(s):

Cerebellar Cortex ◽

Adult Male ◽

Histochemical Study ◽

Monosodium Glutamate ◽

Albino Rats

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The Effect of Induced Diabetes Mellitus on the Cerebellar Cortex of Adult Male Rat and the Possible Protective Role of Oxymatrine: A Histological, Immunohistochemical and Biochemical Study

10.21203/rs.3.rs-300672/v1 ◽

2021 ◽

Author(s):

Amany Mohamed Shalaby ◽

Adel Mohamed Aboregela ◽

Mohamed Ali Alabiad ◽

Mona Tayssir Sadek

Keyword(s):

Diabetes Mellitus ◽

Purkinje Cells ◽

Cerebellar Cortex ◽

Adult Male ◽

Protective Role ◽

Diabetic Rats ◽

Male Rats ◽

Group I ◽

Group Ii

Abstract Diabetes mellitus (DM) represents a widespread metabolic disease with a well-known neurotoxicity in both central and peripheral nervous systems. Oxymatrine is a traditional Chinese herbal medicine that has various pharmacological activities including; anti-oxidant, anti-apoptotic and anti-inflammatory potentials. The present work aimed to study the impact of diabetes mellitus on the cerebellar cortex of adult male albino rat and to evaluate the potential protective role of oxymatrine using different histological methods. Fifty-five adult male rats were randomly divided into three groups: group I served as control, group II was given oxymatrine (80 mg/kg/day) orally for 8 weeks and group III was given a single dose of streptozotocin (50mg/kg) intaperitoneally to induce diabetes. Then diabetic rats were subdivided into two subgroups: subgroup IIIa that received no additional treatment and subgroup IIIb that received oxymatrine similar to group II. The diabetic group revealed numerous changes in the Purkinje cell layer in the form of multilayer arrangement of Purkinje cells, shrunken cells with deeply stained nuclei as well as focal loss of the Purkinje cells. A significant increment in GFAP and synaptophysin expression was reported. Transmission electron microscopy showed irregularity and splitting of myelin sheaths in the molecular layer, dark shrunken Purkinje cells with ill-defined nuclei, dilated Golgi saccules and dense granule cells with irregular nuclear outlines in the granular layer. In contrast, these changes were less evident in diabetic rats that received oxymatrine. In conclusion, Oxymatrine could protect the cerebellar cortex against changes induced by DM.

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