Ex Vivo Transfer of the Hoxc-8-interacting Domain of Smad1 by a Tropism-modified Adenoviral Vector Results in Efficient Bone Formation in a Rabbit Model of Spinal Fusion

Silane-coating strategy has been used to bind biological compounds to the titanium surface, thereby making implant devices biologically active. However, it has not been determined if the presence of the silane coating itself is biocompatible to osseointegration. The aim of the present study was to evaluate if silane-coating affects bone formation on titanium using a rabbit model. For this, titanium screw implants (3.75 by 6 mm) were hydroxylated in a solution of H2SO4/30% H2O2 for 4 h before silane-coating with 3-aminopropyltriethoxysilane (APTES). A parallel set of titanium screws underwent only the hydroxylation process to present similar acid-etched topography as a control. The presence of the silane on the surface was checked by x-ray photoelectron spectroscopy (XPS), with scanning electron microscopy (SEM) and atomic force microscopy (AFM). A total of 40 titanium screws were implanted in the tibia of ten New Zealand rabbits in order to evaluate bone-to-implant contact (BIC) after 3 weeks and 6 weeks of healing. Silane-coated surface presented higher nitrogen content in the XPS analysis, while micro- and nano-topography of the surface remained unaffected. No difference between the groups was observed after 3 and 6 weeks of healing (p > 0.05, independent t-test), although an increase in BIC occurred over time. These results indicate that silanization of a titanium surface with APTES did not impair the bone formation, indicating that this can be a reliable tool to anchor osteogenic molecules on the surface of implant devices.

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Early Spatial and Temporal Events of Human T-Lymphotropic Virus Type 1 Spread following Blood-Borne Transmission in a Rabbit Model of Infection

Journal of Virology ◽

10.1128/jvi.01537-09 ◽

2010 ◽

Vol 84 (10) ◽

pp. 5124-5130 ◽

Cited By ~ 2

Author(s):

Rashade A. H. Haynes ◽

Bevin Zimmerman ◽

Laurie Millward ◽

Evan Ware ◽

Christopher Premanandan ◽

...

Keyword(s):

Virus Type ◽

Ex Vivo ◽

Rabbit Model ◽

Virus Detection ◽

Mononuclear Leukocytes ◽

T Lymphotropic Virus ◽

Viral Spread ◽

Temporal Events

ABSTRACT Human T-lymphotropic virus type 1 (HTLV-1) infection causes adult T-cell leukemia/lymphoma (ATL) and is associated with a variety of lymphocyte-mediated disorders. HTLV-1 transmission occurs by transmission of infected cells via breast-feeding by infected mothers, sexual intercourse, and contaminated blood products. The route of exposure and early virus replication events are believed to be key determinants of virus-associated spread, antiviral immune responses, and ultimately disease outcomes. The lack of knowledge of early events of HTLV-1 spread following blood-borne transmission of the virus in vivo hinders a more complete understanding of the immunopathogenesis of HTLV-1 infections. Herein, we have used an established animal model of HTLV-1 infection to study early spatial and temporal events of the viral infection. Twelve-week-old rabbits were injected intravenously with cell-associated HTLV-1 (ACH-transformed R49). Blood and tissues were collected at defined intervals throughout the study to test the early spread of the infection. Antibody and hematologic responses were monitored throughout the infection. HTLV-1 intracellular Tax and soluble p19 matrix were tested from ex vivo cultured lymphocytes. Proviral copy numbers were measured by real-time PCR from blood and tissue mononuclear leukocytes. Our data indicate that intravenous infection with cell-associated HTLV-1 targets lymphocytes located in both primary lymphoid and gut-associated lymphoid compartments. A transient lymphocytosis that correlated with peak virus detection parameters was observed by 1 week postinfection before returning to baseline levels. Our data support emerging evidence that HTLV-1 promotes lymphocyte proliferation preceding early viral spread in lymphoid compartments to establish and maintain persistent infection.

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Volumetric MicroCT Intensity Histograms of Fatty Infiltration Correlate with the Mechanical Strength of Rotator Cuff Repairs: An Ex Vivo Rabbit Model

Clinical Orthopaedics and Related Research ◽

10.1097/corr.0000000000001540 ◽

2020 ◽

Vol Publish Ahead of Print ◽

Author(s):

Phillip E. McClellan ◽

Lekha Kesavan ◽

Yujing Wen ◽

Jason Ina ◽

Derrick M. Knapik ◽

...

Keyword(s):

Rotator Cuff ◽

Mechanical Strength ◽

Ex Vivo ◽

Fatty Infiltration ◽

Rabbit Model

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Postoperative bisphosphonate do not significantly alter the fusion rate after lumbar spinal fusion: a meta-analysis

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-021-02444-z ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Jun Mei ◽

Xiaoxu Song ◽

Xiaoming Guan ◽

Dou Wu ◽

Junjie Wang ◽

...

Keyword(s):

Bone Formation ◽

Spinal Fusion ◽

Meta Analysis ◽

Fusion Rate ◽

Lumbar Spinal Fusion ◽

Fusion Surgery ◽

Number Of Patients ◽

Cage Subsidence ◽

Lumbar Spinal

Abstract Background To evaluate the effect of postoperative BP treatment on improving the fusion rate after lumbar spinal fusion surgery by performing a meta-analysis of randomized controlled trials (RCTs) and other comparative cohort studies. Methods A comprehensive search of PubMed, EMBASE, the Web of Science, and the Cochrane Central Register of Controlled Trials was performed for RCTs and other comparative cohort studies on the effect of BP treatment on improving the fusion rate after lumbar spinal fusion surgery. The primary outcome measures were the number of patients with bone formation grades A, B, and C at 12 months of follow-up; fusion rates at 12 and 24 months of follow-up; vertebral compression fracture (VCF) at 12 and 24 months of follow-up; pedicle screw loosening at 24 months of follow-up; and cage subsidence, the Oswestry disability index (ODI), and the visual analogue score (VAS) at 12 months of follow-up. The final search was performed in July 2020. Results Seven studies with 401 patients were included. Compared with the placebo, BP treatment did not significantly alter the number of patients with bone formation grades A, B, and C, or the VAS at the 12-month follow-up or the fusion rates at the 12- and 24-month follow-ups. In addition, compared with the placebo, BPs significantly reduced the risks of VCF at the 12- and 24-month follow-ups, pedicle screw loosening at the 24-month follow-up, and cage subsidence and the ODI at the 12-month follow-up. Conclusions Postoperative BPs do not clearly improve bone formation and the fusion rate, but they reduce VCF, cage subsidence, and loosening of pedicle screws after lumbar fusion surgery compared with the control treatment.

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Cervical spinal fusion enabled by a ceramic implant in an animal model; ex vivo CT micromotion analysis

Brain and Spine ◽

10.1016/j.bas.2021.100168 ◽

2021 ◽

Vol 1 ◽

pp. 100168

Author(s):

Maria Östman ◽

Peter Försth ◽

Håkan Engqvist ◽

Michael Pujari-Palmer ◽

Franck Forterre ◽

...

Keyword(s):

Animal Model ◽

Spinal Fusion ◽

Ex Vivo ◽

Ceramic Implant

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New Bone Formation in Tuberculous-Infected Vertebral Body Defect after Administration of Bone Marrow Stromal Cells in Rabbit Model

Asian Spine Journal ◽

10.4184/asj.2016.10.1.1 ◽

2016 ◽

Vol 10 (1) ◽

pp. 1 ◽

Cited By ~ 6

Author(s):

Ahmad Jabir Rahyussalim ◽

Tri Kurniawati ◽

Nurjati Chairani Siregar ◽

Agus Syahrurachman ◽

Ismail Hadisubroto Dilogo ◽

...

Keyword(s):

Bone Marrow ◽

Bone Formation ◽

Vertebral Body ◽

Stromal Cells ◽

Bone Marrow Stromal Cells ◽

Rabbit Model ◽

Marrow Stromal Cells ◽

New Bone Formation

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Irregular bone defect repair via tissue-engineered periosteum approach in rabbit model.

10.21203/rs.2.19343/v1 ◽

2019 ◽

Author(s):

Lin Zhao ◽

Jia-Jia Yu ◽

Cangyu Zhang ◽

Xiuhui Wang

Keyword(s):

Bone Formation ◽

Bone Defect ◽

Rabbit Model ◽

Long Bone ◽

3D Scaffold ◽

Promising Alternative ◽

Bone Defect Repair ◽

Defect Repair ◽

Group 3 ◽

Group 2

Abstract Background As an alternative of bone grafts for defect repair, tissue engineering is much promising for clinical application. In previous studies, we have succeeded in repair of long bone defect with homemade tissue-engineered periosteum (TEP), of which is fabraicated by incorporating osteogenically induced mesenchymal stem cells (MSCs) of rabbits with a scaffold of small intestinal submucosa (SIS).Methods In this study, we are aimed to discuss the feasibility of allogenic irregular bone defect repair with the TEP. Thirty-six rabbits whose scapulas were subtotally resected to establish large irregular bone defects model in allogenic rabbits. The defects were treat respectively with TEP (Group 1, n=12), allogenic deproteinized bone (DPB) (Group 2, n=12) and hybrid of TEP and DPB (Group 3, n=12). At 4, 8, and 12 weeks after surgery, the rabbits were sacrificed, and the implants were harvested. X-ray radiographic and histological examinations were performed.ResultsThe findings suggested that the radiographic score in TEP-DPB hybrided implantation (Group 3) was higher than TEP or DPB grafting only (p<0.05).But that was inconsistent with histological findings, which Group1 appeared to possess significantly higher bone formation than Group 2 (p<0.05) and Group3 has higher new bone volume than that of Group 2 (p<0.05).Conclusion We conclude that TEP is a promising alternative in repair of large irregular bone defect.DPB served as a 3D scaffold in combining TEP could provide mechanical support and shaping guide, but hinder new bone formation via TEP approach due to retard degradation.

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