ATL

ObjectiveThis study aimed to assess the relationship between BRCA1 gene methylation, PD-L1 protein expression, and the clinicopathologic features of sporadic ovarian cancer (OC).MethodsBisulfite pyrosequencing and immunohistochemistry were used to detect BRCA1 gene methylation and PD-L1 protein expression, respectively, in tumor tissues from 112 patients with sporadic OC. Their levels were analyzed against clinicopathologic characteristics and prognosis using standard statistical methods.ResultsTwenty percent (22/112) of the OC cases exhibited BRCA1 gene hypermethylation. The frequency of BRCA1 hypermethylation was significantly higher in serous OC (25%) than in nonserous OC (8%; P < 0.05). No significant correlations were discovered between BRCA1 hypermethylation and age, menstrual status, tumor location, stage, lymph node metastasis, and prognosis (P > 0.05). Among the 112 OC cases, 59% (66/112) cases were positive for PD-L1 protein expression. No significant difference existed between PD-L1 expression and age, menstrual status, histological type, tumor location, stage, lymph node metastasis, and prognosis (P > 0.05). Moreover, no correlation existed between BRCA1 methylation and PD-L1 expression (P > 0.05, r = 0.002).ConclusionsThis is the first study linking BRCA1 hypermethylation variability to PD-L1 protein expression and the clinicopathologic features of OC. The data demonstrated that an epigenetic alteration of BRCA1 was closely associated with serous OC. The expression of PD-L1 was unrelated to the clinicopathologic features or BRCA1 hypermethylation in sporadic OC.

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Impacts Of Tumor Location On Prognosis Of Patients With Gallbladder Carcinoma

10.21203/rs.3.rs-41650/v1 ◽

2020 ◽

Author(s):

Zhencheng Zhu ◽

Kunlun Luo ◽

Qingzhou Zhu ◽

Weixuan Xie

Keyword(s):

Survival Analysis ◽

Lymph Node ◽

Bile Duct ◽

Liver Metastasis ◽

Lymph Node Metastasis ◽

Gallbladder Carcinoma ◽

Tumor Location ◽

Node Metastasis ◽

Significant Difference ◽

Bile Duct Invasion

Abstract Objective: To investigate the impacts of tumor location on the prognosis of patients with T1-3N0-1M0 gallbladder carcinoma(GBC) after radical surgery.Methods: Totally, 136 patients with stage T1-3 gallbladder carcinoma after radical surgery from 2000 to 2018 were enrolled and divided into two groups according to anatomic location of GBC (neck /body and fundus). The clinicopathological features and survival time were compared between these two groups. At last, in combination with the difference between the liver side and the peritoneal side of the tumor, survival analysis and multivariable Cox-proportional hazards regression models were performed in GBC patients with survival differences between gallbladder neck and body/fundus tumors.Results: The bile duct invasion, lymph node metastasis, tumor growth pattern, jaundice, albumin, and tumor markers were significantly related to the tumors in neck of gallbladder(P<0.05). Besides, patients with GBC in body and fundus of gallbladder had a higher rate of appearing microscopic liver metastasis(P<0.05). Survival analysis showed that there was significant difference on patients with stage T2 GBC in different tumor location (neck /body and fundus), but no significant difference on stage T1 and T3. Further combining the differences between the liver side and the peritoneal side of the tumor, tumor location, lymph node metastasis, bile duct invasion, microscopic liver metastasis, tumor differentiation, and jaundice were deemed as prognostic factors according to univariable survival analysis. Among these factors, multivariable Cox analysis showed that lymph node metastasis and tumor location were independent prognostic factors for survival of patients with T2 GBC (P <0.05).Conclusions: Tumor location is an important prognostic factor for GBC, especially for the patients with T2 stage. Besides the survival differences between the hepatic-side and peritoneal-side tumors, tumor in neck is also one of the factors predicting the poor prognosis at T2 stage. GBC in neck was more prone to cause bile duct invasion, lymph node metastasis and jaundice. However, tumors in body and fundus were more likely to appear microscopic liver metastasis. Further refinement of the surgery for T2 GBC according to the tumor location may improve their survival time.

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Impact of c-erbB-2 Protein on 5-year Survival Rate of Gastric Cancer Patients after Surgery: A Cohort Study and Meta-analysis

Tumori Journal ◽

10.5301/tj.5000444 ◽

2015 ◽

Vol 103 (3) ◽

pp. 249-254 ◽

Cited By ~ 4

Author(s):

Hao Wu ◽

Zhenzhai Cai ◽

Guangrong Lu ◽

Shuguang Cao ◽

He Huang ◽

...

Keyword(s):

Gastric Cancer ◽

Lymph Node ◽

Survival Rate ◽

Lymph Node Metastasis ◽

Protein Expression ◽

Meta Analysis ◽

Clinicopathological Characteristics ◽

Node Metastasis ◽

Positive Expression ◽

Significant Difference

Objective To explore the association of c-erbB-2 protein expression with clinicopathological characteristics and prognosis of gastric cancer (GC) after surgery. Methods A total of 133 patients undergoing surgical resection for GC between March 2006 and January 2009 in the Second Affiliated Hospital of Wenzhou Medical University were included in this study. c-erbB-2 protein expression was determined by immunohistochemistry. Afterwards, a meta-analysis was performed to further confirm the association between c-erbB-2 protein expression and GC by employing stringent inclusion and exclusion criteria. All data analyses were conducted with STATA 12.0 and SPSS 19.0. Results There was no significant difference in c-erbB-2 expression among patients with various parameters including age, gender and histological types (all p>0.05). Among 133 GC patients, 32 patients presented c-erbB-2-positive expression and 101 presented c-erbB-2-negative expression (24.1% vs. 75.9%). The c-erbB-2-positive expression rate was significantly higher in GC tissues of patients with lymph node metastasis than those without. Similarly, a significant increase in c-erbB-2 expression was observed in well/moderately differentiated GC tissues compared with poorly differentiated GC. Patients with negative c-erbB-2 expression had a higher 5-year survival rate than those with positive c-erbB-2 expression, which was consistent with the results of the meta-analysis (OR = 0.54, 95% CI 0.37-0.80, p = 0.002). Conclusions Our study demonstrated that high expression of c-erbB-2 protein was strongly associated with lymph node metastasis, histological differentiation and 5-year survival rate in GC patients after surgery.

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Comparison of Postoperative Adjuvant Chemotherapy and Concurrent Chemoradiotherapy for FIGO2018 Stage IIIC1 Cervical Cancer: A Retrospective Study

Medicina ◽

10.3390/medicina57060548 ◽

2021 ◽

Vol 57 (6) ◽

pp. 548

Author(s):

Masahiro Kagabu ◽

Takayuki Nagasawa ◽

Shunsuke Tatsuki ◽

Yasuko Fukagawa ◽

Hidetoshi Tomabechi ◽

...

Keyword(s):

Cervical Cancer ◽

Lymph Node ◽

Adjuvant Chemotherapy ◽

Lymph Node Metastasis ◽

Radical Hysterectomy ◽

Concurrent Chemoradiotherapy ◽

Postoperative Adjuvant Chemotherapy ◽

Node Metastasis ◽

Significant Difference ◽

Advanced Stages

Background and Objectives: In October 2018, the International Federation of Gynecology and Obstetrics (FIGO) revised its classification of advanced stages of cervical cancer. The main points of the classification are as follows: stage IIIC is newly established; pelvic lymph node metastasis is stage IIIC1; and para-aortic lymph node metastasis is stage IIIC2. Currently, in Japan, radical hysterectomy is performed in advanced stages IA2 to IIB of FIGO2014, and concurrent chemoradiotherapy (CCRT) is recommended for patients with positive lymph nodes. However, the efficacy of CCRT is not always satisfactory. The aim of this study was to compare postoperative adjuvant chemotherapy (CT) and postoperative CCRT in stage IIIC1 patients. Materials and Methods: Of the 40 patients who had undergone a radical hysterectomy at Iwate Medical University between January 2011 and December 2016 and were pathologically diagnosed as having positive pelvic lymph nodes, 21 patients in the adjuvant CT group and 19 patients in the postoperative CCRT group were compared. Results: The 5 year survival rates were 77.9% in the CT group and 74.7% in the CCRT group, with no significant difference. There was no significant difference in overall survival or progression-free survival between the two groups. There was no significant difference between CT and CCRT in postoperative adjuvant therapy in the new classification IIIC1 stage. Conclusions: The results of the prospective Japanese Gynecologic Oncology Group (JGOG) 1082 study are pending, but the present results suggest that CT may be a treatment option in rural areas where radiotherapy facilities are limited.

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HMGA1 and HMGA2 protein expression correlates with advanced tumour grade and lymph node metastasis in pancreatic adenocarcinoma

Histopathology ◽

10.1111/j.1365-2559.2011.04121.x ◽

2012 ◽

Vol 60 (3) ◽

pp. 397-404 ◽

Cited By ~ 54

Author(s):

Salvatore Piscuoglio ◽

Inti Zlobec ◽

Pierlorenzo Pallante ◽

Romina Sepe ◽

Francesco Esposito ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Protein Expression ◽

Pancreatic Adenocarcinoma ◽

Tumour Grade ◽

Node Metastasis ◽

Hmga2 Protein

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Relationship between Protein Expression of VEGF-C, MMP-2 and Lymph Node Metastasis in Papillary Thyroid Cancer

Journal of International Medical Research ◽

10.1177/147323000803600411 ◽

2008 ◽

Vol 36 (4) ◽

pp. 699-703 ◽

Cited By ~ 30

Author(s):

X Tian ◽

M Cong ◽

W Zhou ◽

J Zhu ◽

Q Liu

Keyword(s):

Thyroid Cancer ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Protein Expression ◽

Papillary Thyroid Cancer ◽

Papillary Thyroid ◽

Node Metastasis

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Extracellular matrix changes in colorectal carcinoma and correlation with lymph node metastasis

10.1101/2021.01.05.425386 ◽

2021 ◽

Author(s):

Thérèse Rachell Theodoro ◽

Rodrigo Lorenzetti Serrano ◽

Karine Corcione Turke ◽

Sarhan Sydney Saad ◽

Marcelo Augusto Fontenelle Ribeiro Junior ◽

...

Keyword(s):

Extracellular Matrix ◽

Lymph Node ◽

Lymph Node Metastasis ◽

Colorectal Carcinoma ◽

Protein Expression ◽

Cross Sectional ◽

Tissue Samples ◽

Node Metastasis ◽

Mrna And Protein Expression ◽

Neoplastic Tissues

AbstractThe process of proliferation and invasion of tumor cells depends on changes in the extracellular matrix (ECM) through the activation of enzymes and alterations in the profile of ECM components. We aimed to investigate the mRNA and protein expression of ECM components such as heparanase (HPSE), heparanase-2 (HPSE2), matrix metalloproteinase-9 (MMP-9), and syndecan-1 (SYND1) in neoplastic and non-neoplastic tissues of patients with colorectal carcinoma (CRC). It is a cross-sectional study in which twenty-four adult patients that had CRC were submitted to resection surgery. We analyzed the expression of HPSE, HPSE2, MMP-9, and SYND1 by quantitative RT-PCR and immunohistochemistry. Differing from most of the studies that compare the mRNA expression between tumor samples and non-neoplastic tissues, we decided to investigate whether variations exist in the expression of the ECM components between the affected tissue and nontumoral tissue collected from the same patient with CRC. We removed both tissue samples immediately after the surgical resection of CRC. The data showed higher mRNA and protein expression of HPSE2 (P = 0.0058), MMP-9 (P = 0.0268), and SYND1 (P = 0.0002) in tumor samples compared to the non-neoplastic tissues, while there was only an increase in the level of HPSE protein in tumor tissues. A greater expression of HPSE2 was observed in patients with lymph node metastasis (P = 0.048), suggesting that such protein can be a marker of lymph node metastasis in CRC.

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