Incidental Microscopic Granulosa Cell Proliferations

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

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The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073209 ◽

1973 ◽

Vol 31 ◽

pp. 552-553

Author(s):

T. M. Crisp ◽

F.R. Denys

Keyword(s):

Fine Structure ◽

Granulosa Cell ◽

Cell Cultures ◽

Single Injection ◽

Surrounding Medium ◽

Petri Dish ◽

Female Rats ◽

Vaginal Smear ◽

Immature Female ◽

Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

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Rat ovarian insulin-like growth factor binding protein-4: A hormone-dependent granulosa cell-derived antigonadotropin

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)00020-8 ◽

1997 ◽

Vol 4 (3) ◽

pp. 144-151 ◽

Cited By ~ 4

Author(s):

L PUTOWSKI ◽

R ROHAN ◽

D CHOI ◽

W SCHERZER ◽

E RICCIARELLI ◽

...

Keyword(s):

Growth Factor ◽

Granulosa Cell ◽

Binding Protein ◽

Insulin Like Growth Factor ◽

Factor Binding

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Characterization of oxytocin receptor in a human granulosa cell line

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86348-4 ◽

1998 ◽

Vol 5 (1) ◽

pp. 106A-106A

Author(s):

J COPLAND ◽

M ZLATMK ◽

K IVES ◽

M SOLOFF

Keyword(s):

Cell Line ◽

Granulosa Cell ◽

Oxytocin Receptor ◽

Human Granulosa Cell

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INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

Acta Endocrinologica ◽

10.1530/acta.0.0380545 ◽

1961 ◽

Vol 38 (4) ◽

pp. 545-562 ◽

Cited By ~ 2

Author(s):

L. Kecskés ◽

F. Mutschler ◽

I. Glós ◽

E. Thán ◽

I. Farkas ◽

...

Keyword(s):

Pregnant Women ◽

Granulosa Cell ◽

Iron Content ◽

List Type ◽

Granulosa Cell Tumour ◽

Hydrolysis Of ◽

Phenol Fraction ◽

Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

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STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

Acta Endocrinologica ◽

10.1530/acta.0.xxxii0041 ◽

1959 ◽

Vol XXXII (I) ◽

pp. 41-53 ◽

Cited By ~ 8

Author(s):

Stig Kullander ◽

Bengt Källén

Keyword(s):

Tissue Culture ◽

Granulosa Cell ◽

In Vitro Study ◽

Vitro Study ◽

Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

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NEW VIEWS ON THE HORMONE-PRODUCING MESENCHYMOMAS OF THE OVARIES

Acta Endocrinologica ◽

10.1530/acta.0.xxxv0513 ◽

1960 ◽

Vol XXXV (IV) ◽

pp. 513-517

Author(s):

W. P. Plate

Keyword(s):

Granulosa Cell ◽

Sertoli Cell ◽

Leydig Cell ◽

Cell Tumour ◽

Theca Cell ◽

Granulosa Cell Tumour ◽

Sertoli Cell Tumours ◽

Leydig Cell Tumours

ABSTRACT The hormone-producing mesenchymomas of the ovaries can be divided into androblastomas and gynaecoblastomas. The former are derived from »male« elements, and consist of Sertoli-cell tumours and Leydig-cell tumours. The latter arise from »female« elements and consist of granulosacell tumours and theca-cell tumours. Sertoli-cell tumours and granulosacell tumours produce oestrogens, while Leydig-cell tumours and theca-cell tumours produce oestrogens or androgens. Histologically, androblastomas and gynaecoblastomas are often difficult to distinguish. Since no »female« elements occur in a testicle, a granulosa-cell tumour in a testicle is improbable. Gynandroblastomas, therefore, can only be found in an ovary.

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