In Vitro Mineralization of Human Mesenchymal Stem Cells on Three-Dimensional Type I Collagen versus PLGA Scaffolds: A Comparative Analysis

2011 ◽  
Vol 127 (6) ◽  
pp. 2301-2311 ◽  
Author(s):  
Erwin A. Kruger ◽  
Daniel D. Im ◽  
David S. Bischoff ◽  
Clifford T. Pereira ◽  
Weibiao Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Comparative Analysis ◽  
Type I Collagen ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Type I ◽  
In Vitro Mineralization ◽  
Collagen Versus

Type I collagen promotes proliferation and osteogenesis of human mesenchymal stem cells via activation of ERK and Akt pathways

2010 ◽  
Vol 9999A ◽  
pp. NA-NA ◽  
Author(s):  
Kuo-Shu Tsai ◽  
Shou-Yen Kao ◽  
Chien-Yuan Wang ◽  
Yng-Jiin Wang ◽  
Jung-Pan Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Type I Collagen ◽  
Human Mesenchymal Stem Cells ◽  
Type I

scholarly journals 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton’s Jelly Mesenchymal Stem Cells

Pharmaceutics ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1448
Author(s):  
Maria Camilla Ciardulli ◽  
Joseph Lovecchio ◽  
Pasqualina Scala ◽  
Erwin Pavel Lamparelli ◽  
Tina Patricia Dale ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Type I Collagen ◽  
In Vitro Study ◽  
Wharton’S Jelly ◽  
Controlled Delivery ◽  
Type I ◽  
Wharton's Jelly

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

scholarly journals Three-dimensional printed bone scaffolds: The role of nano/micro-hydroxyapatite particles on the adhesion and differentiation of human mesenchymal stem cells

2017 ◽  
Vol 231 (6) ◽  
pp. 555-564 ◽  
Author(s):  
Marco Domingos ◽  
Antonio Gloria ◽  
Jorge Coelho ◽  
Paulo Bartolo ◽  
Joaquim Ciurana
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphates ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Mechanical Tests ◽  
Engineering Research

Bone tissue engineering is strongly dependent on the use of three-dimensional scaffolds that can act as templates to accommodate cells and support tissue ingrowth. Despite its wide application in tissue engineering research, polycaprolactone presents a very limited ability to induce adhesion, proliferation and osteogenic cell differentiation. To overcome some of these limitations, different calcium phosphates, such as hydroxyapatite and tricalcium phosphate, have been employed with relative success. This work investigates the influence of nano-hydroxyapatite and micro-hydroxyapatite (nHA and mHA, respectively) particles on the in vitro biomechanical performance of polycaprolactone/hydroxyapatite scaffolds. Morphological analysis performed with scanning electron microscopy allowed us to confirm the production of polycaprolactone/hydroxyapatite constructs with square interconnected pores of approximately 350 µm and to assess the distribution of hydroxyapatite particles within the polymer matrix. Compression mechanical tests showed an increase in polycaprolactone compressive modulus ( E) from 105.5 ± 11.2 to 138.8 ± 12.9 MPa (PCL_nHA) and 217.2 ± 21.8 MPa (PCL_mHA). In comparison to PCL_mHA scaffolds, the addition of nano-hydroxyapatite enhanced the adhesion and viability of human mesenchymal stem cells as confirmed by Alamar Blue assay. In addition, after 14 days of incubation, PCL_nHA scaffolds showed higher levels of alkaline phosphatase activity compared to polycaprolactone or PCL_mHA structures.

scholarly journals Basement membrane proteins modulate cell migration on bovine pericardium extracellular matrix scaffold

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Qi Xing ◽  
Mojtaba Parvizi ◽  
Manuela Lopera Higuita ◽  
Leigh G. Griffiths
Keyword(s):  
Stem Cells ◽  
Monoclonal Antibody ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Cell Migration ◽  
Basement Membrane ◽  
Type I Collagen ◽  
Human Mesenchymal Stem Cells ◽  
Bovine Pericardium ◽  
Type I

AbstractNative bovine pericardium (BP) exhibits anisotropy of its surface ECM niches, with the serous surface (i.e., parietal pericardium) containing basement membrane components (e.g., Laminin, Col IV) and the fibrous surface (i.e., mediastinal side) being composed primarily of type I collagen (Col I). Native BP surface ECM niche anisotropy is preserved in antigen removed BP (AR-BP) extracellular matrix (ECM) scaffolds. By exploiting sideness (serous or fibrous surface) of AR-BP scaffolds, this study aims to determine the mechanism by which ECM niche influences human mesenchymal stem cells (hMSCs) migration. Human mesenchymal stem cells (hMSC) seeding on serous surface promoted more rapid cell migration than fibrous surface seeding. Gene analysis revealed that expression of integrin α3 and α11 were increased in cells cultured on serous surface compared to those on the fibrous side. Monoclonal antibody blockade of α3β1 (i.e., laminin binding) inhibited early (i.e. ≤ 6 h) hMSC migration following serous seeding, while having no effect on migration of cells on the fibrous side. Blockade of α3β1 resulted in decreased expression of integrin α3 by cells on serous surface. Monoclonal antibody blockade of α11β1 (i.e., Col IV binding) inhibited serous side migration at later time points (i.e., 6–24 h). These results confirmed the role of integrin α3β1 binding to laminin in mediating early rapid hMSCs migration and α11β1 binding to Col IV in mediating later hMSCs migration on the serous side of AR-BP, which has critical implications for rate of cellular monolayer formation and use of AR-BP as blood contacting material for clinical applications.

scholarly journals Microencapsulation of cellular aggregate composed of differentiated insulin and glucagon-producing cells from human mesenchymal stem cells derived from adipose tissue.

2020 ◽  
Author(s):  
Claudia Jara ◽  
Felipe Oyarzun-Ampuero ◽  
Flavio Carrión ◽  
Esteban González-Echeverría ◽  
Claudio Cappelli ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Glycemic Control ◽  
Sodium Alginate ◽  
Type I Diabetes ◽  
Human Mesenchymal Stem Cells ◽  
Autoimmune Response ◽  
Type I ◽  
Cellular Aggregates

Abstract Background. In type I diabetes mellitus (T1DM) pancreatic β cells are destroyed. Treatment entails exogenous insulin administration and strict diet control, yet optimal glycemic control is hardly attainable. Islet transplant could be an alternative in patients with poor glycemic control, but inefficient islet purification and autoimmune response of patients still a challenge. Methods Human adipose-derived mesenchymal stem cells (hASC) obtained from lipoaspirated fat tissue from human donors were differentiated in vitro to insulin (Ins) and glucagon (Gcg) producing cells (IPC and GPC respectively). Then, we cocultured IPC and GPC cells in low adhesion conditions to form cellular aggregates, which were encapsulated in a sodium alginate polymer. Expression of pancreatic lineage markers and secretion of insulin or glucagon in vitro were analyzed. Results We demonstrated that multipotent hASC efficiently differentiate to IPC and GPC, which also express pancreatic markers, including insulin or glucagon hormones. In turn, we calculated the Feret diameter of cellular aggregates, finding mean diameters ~80 µm at 72h of incubation. IPC/GPC aggregates were then microencapsulated in sodium-alginate polymer microgels, which were found to be more stable in Ba 2+ stabilized microgels, with average diameters ~300 µm. Interestingly, Ba 2+ -microencapsulated aggregates respond to high external glucose with insulin secretion. Conclusions The IPC/GPC differentiation process from hASC followed by generate cellular aggregates in vitro, that once microencapsulated could represent a possible treatment to T1DM.

scholarly journals Hijacking the Cellular Mail: Exosome Mediated Differentiation of Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Raghuvaran Narayanan ◽  
Chun-Chieh Huang ◽  
Sriram Ravindran
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Cell Fate ◽  
Type I Collagen ◽  
Stem Cell Differentiation ◽  
Future Research ◽  
Type I ◽  
Specific Differentiation

Bone transplantation is one of the most widely performed clinical procedures. Consequently, bone regeneration using mesenchymal stem cells and tissue engineering strategies is one of the most widely researched fields in regenerative medicine. Recent scientific consensus indicates that a biomimetic approach is required to achieve proper regeneration of any tissue. Exosomes are nanovesicles secreted by cells that act as messengers that influence cell fate. Although exosomal function has been studied with respect to cancer and immunology, the role of exosomes as inducers of stem cell differentiation has not been explored. We hypothesized that exosomes can be used as biomimetic tools for regenerative medicine. In this study we have explored the use of cell-generated exosomes as tools to induce lineage specific differentiation of stem cells. Our results indicate that proosteogenic exosomes isolated from cell cultures can induce lineage specific differentiation of naïve MSCsin vitroandin vivo. Additionally, exosomes can also bind to matrix proteins such as type I collagen and fibronectin enabling them to be tethered to biomaterials. Overall, the results from this study show the potential of cell derived exosomes in bone regenerative medicine and opens up new avenues for future research.

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