scholarly journals Cultures of "Clostridium acetobutylicum" from Various Collections Comprise Clostridium acetobutylicum, Clostridium beijerinckii, and Two Other Distinct Types Based on DNA-DNA Reassociation

1997 ◽  
Vol 47 (2) ◽  
pp. 420-424 ◽  
Author(s):  
J. L. JOHNSON ◽  
J. TOTH ◽  
S. SANTIWATANAKUL ◽  
J.-S. CHEN
Keyword(s):  
Clostridium Acetobutylicum ◽  
Clostridium Beijerinckii ◽  
Dna Reassociation

scholarly journals Metabolic Engineering of Clostridium acetobutylicum ATCC 824 for Isopropanol-Butanol-Ethanol Fermentation

2011 ◽  
Vol 78 (5) ◽  
pp. 1416-1423 ◽  
Author(s):  
Joungmin Lee ◽  
Yu-Sin Jang ◽  
Sung Jun Choi ◽  
Jung Ae Im ◽  
Hyohak Song ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Clostridium Acetobutylicum ◽  
Secondary Alcohol ◽  
Clostridium Beijerinckii ◽  
Fuel Additive ◽  
Gas Stripping ◽  
Alcohol Fermentation ◽  
Content Type ◽  
Formation Pathway ◽  
Total Alcohol

ABSTRACTClostridium acetobutylicumnaturally produces acetone as well as butanol and ethanol. Since acetone cannot be used as a biofuel, its production needs to be minimized or suppressed by cell or bioreactor engineering. Thus, there have been attempts to disrupt or inactivate the acetone formation pathway. Here we present another approach, namely, converting acetone to isopropanol by metabolic engineering. Since isopropanol can be used as a fuel additive, the mixture of isopropanol, butanol, and ethanol (IBE) produced by engineeredC. acetobutylicumcan be directly used as a biofuel. IBE production is achieved by the expression of a primary/secondary alcohol dehydrogenase gene fromClostridium beijerinckiiNRRL B-593 (i.e.,adhB-593) inC. acetobutylicumATCC 824. To increase the total alcohol titer, a synthetic acetone operon (actoperon;adc-ctfA-ctfB) was constructed and expressed to increase the flux toward isopropanol formation. When this engineering strategy was applied to the PJC4BK strain lacking in thebukgene (encoding butyrate kinase), a significantly higher titer and yield of IBE could be achieved. The resulting PJC4BK(pIPA3-Cm2) strain produced 20.4 g/liter of total alcohol. Fermentation could be prolonged byin situremoval of solvents by gas stripping, and 35.6 g/liter of the IBE mixture could be produced in 45 h.

Transcriptomic studies of solventogenic clostridia, Clostridium acetobutylicum and Clostridium beijerinckii

2021 ◽  
pp. 107889
Author(s):  
Patakova Petra ◽  
Branska Barbora ◽  
Vasylkivska Maryna ◽  
Jureckova Katerina ◽  
Musilova Jana ◽  
...  
Keyword(s):  
Clostridium Acetobutylicum ◽  
Clostridium Beijerinckii ◽  
Solventogenic Clostridia ◽  
Transcriptomic Studies

scholarly journals d-2,3-Butanediol Production Due to Heterologous Expression of an Acetoin Reductase in Clostridium acetobutylicum

2011 ◽  
Vol 77 (8) ◽  
pp. 2582-2588 ◽  
Author(s):  
Marco A. J. Siemerink ◽  
Wouter Kuit ◽  
Ana M. López Contreras ◽  
Gerrit Eggink ◽  
John van der Oost ◽  
...  
Keyword(s):  
Experimental Data ◽  
Heterologous Expression ◽  
Metabolic Network ◽  
Clostridium Acetobutylicum ◽  
Clostridium Beijerinckii ◽  
Racemic Mixture ◽  
Gene Encoding ◽  
Content Type ◽  
Batch Cultures

ABSTRACTAcetoin reductase (ACR) catalyzes the conversion of acetoin to 2,3-butanediol. Under certain conditions,Clostridium acetobutylicumATCC 824 (and strains derived from it) generates bothd- andl-stereoisomers of acetoin, but because of the absence of an ACR enzyme, it does not produce 2,3-butanediol. A gene encoding ACR fromClostridium beijerinckiiNCIMB 8052 was functionally expressed inC. acetobutylicumunder the control of two strong promoters, the constitutivethlpromoter and the late exponentialadcpromoter. Both ACR-overproducing strains were grown in batch cultures, during which 89 to 90% of the natively produced acetoin was converted to 20 to 22 mMd-2,3-butanediol. The addition of a racemic mixture of acetoin led to the production of bothd-2,3-butanediol andmeso-2,3-butanediol. A metabolic network that is in agreement with the experimental data is proposed. Native 2,3-butanediol production is a first step toward a potential homofermentative 2-butanol-producing strain ofC. acetobutylicum.

Isolation of Clostridium acetobutylicum strains and the preliminary investigation of the hemicellulolytic activities of isolate 3BYR

1992 ◽  
Vol 38 (11) ◽  
pp. 1120-1127 ◽  
Author(s):  
D. G. Trudeau ◽  
R. L. Bernier ◽  
D. J. Gannon ◽  
C. W. Forsberg
Keyword(s):  
Carbon Source ◽  
Clostridium Acetobutylicum ◽  
National Park ◽  
Glucuronic Acid ◽  
Preliminary Investigation ◽  
Clostridium Beijerinckii ◽  
Xylan Hydrolysis ◽  
Glucuronidase Activity ◽  
Very High ◽  
Selection Of

Thirty-nine xylanolytic bacteria tentatively identified as Clostridia were isolated from a selection of agricultural and forest soil samples, and water–sediment–fibre samples from acidic springs in the Yellowstone National Park. Screening for xylan hydrolysis was performed using an enriched agar medium at pH 5.5 with Remazol–xylan as the indicator, with or without an initial enrichment using xylan as the major carbon source. From 13 of the most highly xylanolytic strains, 9 were tentatively identified as Clostridium acetobutylicum, 2 as Clostridium butyricum, and 2 as Clostridium beijerinckii. The C. acetobutylicum isolate 3BYR utilized 80% of oat spelt xylan as a carbon source during growth. The bacterium exhibited very high extracellular xylanase and xylosidase activities and, as well, α-L-arabinofuranosidase and α-glucuronidase activities. Glucuronidase activity was documented by the release of 4-O-methyl-α-D-glucuronic acid from birchwood xylan. The results of this work indicate the ubiquity of xylanolytic Clostridia, and that the previously unreported activity, α-glucuronidase, has been demonstrated in C. acetobutylicum. Key words: α-glucuronidase, xylanase, Clostridium acetobutylicum, xylan.

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