Isolation of Clostridium acetobutylicum strains and the preliminary investigation of the hemicellulolytic activities of isolate 3BYR

1992 ◽  
Vol 38 (11) ◽  
pp. 1120-1127 ◽  
Author(s):  
D. G. Trudeau ◽  
R. L. Bernier ◽  
D. J. Gannon ◽  
C. W. Forsberg
Keyword(s):  
Carbon Source ◽  
Clostridium Acetobutylicum ◽  
National Park ◽  
Glucuronic Acid ◽  
Preliminary Investigation ◽  
Clostridium Beijerinckii ◽  
Xylan Hydrolysis ◽  
Glucuronidase Activity ◽  
Very High ◽  
Selection Of

Thirty-nine xylanolytic bacteria tentatively identified as Clostridia were isolated from a selection of agricultural and forest soil samples, and water–sediment–fibre samples from acidic springs in the Yellowstone National Park. Screening for xylan hydrolysis was performed using an enriched agar medium at pH 5.5 with Remazol–xylan as the indicator, with or without an initial enrichment using xylan as the major carbon source. From 13 of the most highly xylanolytic strains, 9 were tentatively identified as Clostridium acetobutylicum, 2 as Clostridium butyricum, and 2 as Clostridium beijerinckii. The C. acetobutylicum isolate 3BYR utilized 80% of oat spelt xylan as a carbon source during growth. The bacterium exhibited very high extracellular xylanase and xylosidase activities and, as well, α-L-arabinofuranosidase and α-glucuronidase activities. Glucuronidase activity was documented by the release of 4-O-methyl-α-D-glucuronic acid from birchwood xylan. The results of this work indicate the ubiquity of xylanolytic Clostridia, and that the previously unreported activity, α-glucuronidase, has been demonstrated in C. acetobutylicum. Key words: α-glucuronidase, xylanase, Clostridium acetobutylicum, xylan.

Der Sommer und Herbst 2003 aus phänologischer Sicht | Summer and autumn 2003 from a phenological point of view

2004 ◽  
Vol 155 (5) ◽  
pp. 142-145 ◽  
Author(s):  
Claudio Defila
Keyword(s):  
Time Series ◽  
New Record ◽  
Point Of View ◽  
Late Spring ◽  
Mean Deviation ◽  
Leaf Shedding ◽  
The Mean ◽  
Very High ◽  
Selection Of

The record-breaking heatwave of 2003 also had an impact on the vegetation in Switzerland. To examine its influences seven phenological late spring and summer phases were evaluated together with six phases in the autumn from a selection of stations. 30% of the 122 chosen phenological time series in late spring and summer phases set a new record (earliest arrival). The proportion of very early arrivals is very high and the mean deviation from the norm is between 10 and 20 days. The situation was less extreme in autumn, where 20% of the 103 time series chosen set a new record. The majority of the phenological arrivals were found in the class «normal» but the class«very early» is still well represented. The mean precocity lies between five and twenty days. As far as the leaf shedding of the beech is concerned, there was even a slight delay of around six days. The evaluation serves to show that the heatwave of 2003 strongly influenced the phenological events of summer and spring.

PSXII-2 Identification of candidate SNPs associated with high prolificacy in Romanov sheep

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 254-255
Author(s):  
Alexey V Shakhin ◽  
Arsen V Dotsev ◽  
Tatiana E Deniskova ◽  
Gottfried Brem ◽  
Natalia A Zinovieva
Keyword(s):  
Higher Education ◽  
Genetic Basis ◽  
Reference Genome ◽  
Unique Property ◽  
Candidate Snps ◽  
Merino Sheep ◽  
Sheep Breeds ◽  
Important Trait ◽  
Very High ◽  
Selection Of

Abstract Prolificacy is a very important trait in sheep. Romanov sheep, the well-known Russian sheep breed, are characterized by very high prolificacy; however, the genetic basis of this unique property of Romanov sheep is still unknown. It was reported that Ovine BMPR1B gene, located on the OAR6, is associated with prolificacy in several sheep breeds. The aim of our study was to identify candidate SNPs within BMPR1B gene, related to prolificacy. To achieve this goal, using NGS technology, we sequenced ovine BMPR1B gene in Romanov sheep (n = 6), which are characterized by high prolificacy (about 270 lambs per 100 ewes). The sequences of BMPR1B gene of Noire du Velay, Tan, Southdown and Australian Horned Merino sheep breeds as well as Asiatic mouflon (n = 1), which are characterized by significantly lower prolificacy (from 110 to 180 lambs per 100 ewes) were derived from publicly available sources and used for comparison. FST analysis performed in PLINK 1.9 program revealed 10 SNPs with values higher than 0.8. The majority of candidate SNPs under putative selection were localized in the region from 29,382,098 to 29,430,387 on OAR6 of Ovine reference genome (Oar_v3.1 (Ensembl release 98). Thus, we can suggest, that this region of the BMPR1B gene can be considered as the putative region, associated with high prolificacy of Romanov sheep. Additional studies will be needed to confirm the effect of identified candidate SNPs on prolificacy traits. The research results will be useful for artificial selection of sheep with higher prolific capacity, including the introduction of desired alleles in sheep populations using genome editing technologies. This work was supported by the Russian Ministry of Science and Higher Education No. 0445-2019-0024 and RFBR No. 20-516-56002.

scholarly journals Prey species and prey selection of dholes at three different sites in Thailand

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
KHWANRUTAI CHARASPET ◽  
Ronglarp - Sukmasuang ◽  
NORASET KHOEWSREE ◽  
MANANYA PLA-ARD ◽  
YUWALUK CHANACHAI
Keyword(s):  
National Park ◽  
Prey Species ◽  
Dietary Diversity ◽  
Prey Selection ◽  
Selection Index ◽  
Camera Traps ◽  
Total Biomass ◽  
Wildlife Sanctuary ◽  
Khao Yai National Park ◽  
Selection Of

Abstract. Charaspet K, Sukmasuang R, Khoewsree N, Pla-ard M, Chanachai Y. 2020. Prey species and prey selection of dholes at three different sites in Thailand. Biodiversitas 21: 5248-5262. The study of prey species and prey selection of Dholes at 3 different sites was conducted at Khao Yai National Park, Salak Pra, and Huai Kha Khaeng Wildlife Sanctuaries from 2013 to 2020. Information on Dhole prey at the sites was collected from the residues of dhole scats, from which the selection index, the relative biomass of the prey, and the relative amounts of the consumed prey were calculated. The data were collected simultaneously with the use of camera traps at each site. The study revealed that there were 13 species of Dhole prey with body weight over 5 kg. The result indicated that there were 7 species of even-toed ungulates. The relative biomass of even-toed ungulates ranged between 76.78 - 90.50% of the total biomass of all the Dholes’ consumed prey for all study sites. The dietary diversity index unveiled a similar index in all areas, which proved the adequacy of the analyzed scats. However, the Niche breadth index, which indicates the relevance of prey selection and prey species to the appearances of the prey at each site, was found to be high at Huai Kha Khaeng Wildlife Sanctuary, Khao Yai National Park, while the index was found to be low at Salak Pra Wildlife Sanctuary. The results revealed that Dholes consumed viverrid species and Malayan porcupine more often at the site where there were large carnivores. The recommendation from this study was the conservation and restoration of the ungulate populations, the main prey, as it greatly affects the conservation of the Dhole populations in Thailand.  Grassland and salt lick sites, water sources improvements are also important to promote prey population. The conservation of wildlife prey by releasing them to nature, as currently conducted, has an effect on the increase of Dholes’ prey species.

scholarly journals Screening of local wild-type Xanthomonas spp. for xanthan biosynthesis using media with different carbon sources

2021 ◽  
Vol 26 (4) ◽  
pp. 2800-2807
Author(s):  
IDA ZAHOVIĆ ◽  
JELENA DODIĆ ◽  
SINIŠA MARKOV ◽  
JOVANA GRAHOVAC ◽  
MILA GRAHOVAC ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Carbon Source ◽  
Carbon Sources ◽  
Wild Type ◽  
Biotechnological Production ◽  
Aerobic Conditions ◽  
Pepper Leaves ◽  
Synthetic Media ◽  
Selection Of

In this study the screening of different Xanthomonas strains, isolated from infected crucifers and pepper leaves, for xanthan biosynthesis on semi-synthetic media containing different carbon sources was performed. The success of xanthan biosynthesis was estimated based on xanthan concentration in media and its molecular weight. Glucose and glycerol were investigated as carbon sources in a quantity of 20.0 g/L. Xanthan biosynthesis by different Xanthomonas isolates on two different cultivation media was carried out in Erlenmeyer flasks under aerobic conditions for 168 h. According to the obtained results selection of the carbon source, producing strain and their combination have a statistically significant effect on xanthan quantity and quality. The results obtained in this study indicate that local wild-type Xanthomonas strains isolated from pepper leaves have a great potential for application in biotechnological production of good-quality xanthan on glycerol-based media.

scholarly journals Describing the Mountainsnails (Oreohelix Sp.) of Grand Teton National Park, Wyoming

2013 ◽  
Vol 36 ◽  
pp. 94-97
Author(s):  
Lusha Tronstad ◽  
Gary Beauvais ◽  
Jeanne Serb ◽  
Kevin Roe
Keyword(s):  
National Park ◽  
National Park Service ◽  
Endangered Species Act ◽  
The State ◽  
Recent Past ◽  
Grand Teton National Park ◽  
Important Group ◽  
Park Service ◽  
Very High ◽  
The U.S

Invertebrates are receiving an increasing amount of conservation attention across North America. Currently, about 40% of the animals listed under the U.S. Endangered Species Act (ESA) are invertebrates (www.NatureServe.org). The National Park Service and other agencies require better information on invertebrate faunas in order to effectively conserve this important group of animals. One way to prioritize invertebrate groups for study is to assess the number of rare taxa within a given genus. In this context, Oreohelix (mountainsnails) are a top priority because the genus is assumed to support a very high percentage of rare and endemic taxa. Additionally, Oreohelix species in Wyoming and surrounding states have been petitioned for ESA listing in the recent past. The diversity of Oreohelix forms in Wyoming is not well-understood, and the current taxonomy may not reflect the true pattern of diversity within the state. Therefore, we are studying both the morphology and genetic structure of Oreohelix in Grand Teton National Park to begin to understand the diversity of mountainsnails in the state. We collected Oreohelix from 4 locations in Grand Teton National Park. Based on shell and internal characteristics, all individuals were identified as O. subrudis. We are currently preparing specimens for DNA sequencing.

scholarly journals Selection of non-invasive load procedure in diagnosis of ischemic heart disease (review)

2018 ◽  
pp. 62-70 ◽  
Author(s):  
V. P. Lupanov
Keyword(s):  
Coronary Heart Disease ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Clinical Examination ◽  
Ischemic Heart ◽  
Non Invasive ◽  
Careful Clinical Examination ◽  
Very High ◽  
Selection Of ◽  
High Pretest Probability

The diagnosis of stable ischemic heart disease begins with a careful clinical examination of the patient and non-invasive testing to identify the disease. Patients with very low and very high pretest probability should not undergo various non-invasive tests. Various non-invasive tests are available to assess the presence of coronary heart disease in patients with an intermediate probability of ischemic heart disease (15–65%). The combination of anatomical with functional non-invasive tests helps improve diagnostic capabili of the disease.

scholarly journals Rheological Evaluation of Some Commercial Xylanases

2011 ◽  
Vol 68 (2) ◽  
Author(s):  
Mihai OGNEAN ◽  
Claudia Felicia OGNEAN ◽  
Neli DARIE
Keyword(s):  
Xylanase Activity ◽  
Dough Rheology ◽  
Xylan Hydrolysis ◽  
Selection Of

The arabinoxylans play and the xylanases play an important role breadmaking. For a better selection of xylanases, it is necessary to understand how they work in breadmaking. At the same activity, the xylanases have different effect on dough rheology. The xylanases activity measured through birch xylan hydrolysis it is not correlated with extensographic effects. Weak but better correlations were observed when the xylanase activity was measured as the ability to decrease the viscosity of soluble wheat arabinoxylans solution.

scholarly journals Изучение увологических и агробиологических показателей сорта винограда Кокур белый на различных подвоях для проведения клоновой селекции

2019 ◽  
pp. 105-108
Author(s):  
Nataliya Studennikova ◽  
Zinaida Kotolovets ◽  
Nadezhda Tikhomirova ◽  
Natalia Urdenko
Keyword(s):  
Training System ◽  
Large Percentage ◽  
Individual Selection ◽  
Grapevine Variety ◽  
Points Of View ◽  
Relative Productivity ◽  
Very High ◽  
Production Conditions ◽  
Selection Of

Представлены результаты работы по изучению влияния филлоксероустойчивых подвоев Берландиери × Рипариа Кобер 5ББ и Берландиери × Рипариа Телеки 4Б (СО4) на агробиологические и увологические показатели крымского технического белоягодного аборигенного сорта винограда Кокур белый в условиях Алуштинской долины (филиал «Алушта» ФГУП «ПАО «Массандра»). Для проведения исследований были выбраны: участок № 361, площадью 4,49 га, на котором произрастают кусты винограда Кокур белый, привитые на подвое Берландиери × Рипариа Кобер 5 ББ (2010 год посадки, схема 3 м ×1,25 м) и участок № 391, площадью 3,06 га, на котором возделываются растения сорта Кокур белый, привитые на подвое Берландиери × Рипариа Телеки 4Б (СО4) - 2011 год посадки, схема 3 м × 1 м). На обоих участках система ведения кустов - вертикальная трехпроволочная шпалера, формировка АЗОС-1, высота штамба - 1,3 м. Показана целесообразность проведения работ по улучшению сорта Кокур белый методом клоновой селекции на сортоподвойной комбинации Кокур белый подвой Кобер 5 ББ: у растений отмечен высокий уровень коэффициента плодоношения (0,92), довольно крупные грозди (365 г), урожай с куста в среднем составил 4,74 кг/куст, очень высокая продуктивность побега по сырой массе грозди - 336,6 г/побег, большой процент выхода мякоти и сока - 91,3%. Оценка имеющихся сортоподвойных комбинаций в производственных условиях позволила провести индивидуальный отбор генотипически ценных в биолого-хозяйственном отношении форм растений.The paper reports on the findings of a study on the influence of phylloxera-resistant rootstocks ‘Berlandieri’בRiparia Kober 5BB’ and ‘Berlandieri’בRiparia Teleki 4B (СО4)’ on the agro-biological and uvological indicators of Crimean aboriginal grapevine variety used in winemaking ‘Kokur Beliy in the conditions of Alushta valley (branch of Alushta FGUP PAO Massandra). The following were selected for research: plot № 361 with an area of 4.49 hectares with bushes of ‘Kokur Beliy’ grafted on rootstock of ‘Berlandieri’ × Riparia Kobera 5 BB’ (planting year - 2010, planting scheme 3 m ×1.25 m) and plot № 391 covering 3.06 hectares with ‘Kokur Beliy’ vines grafted on ‘Berlandieri’ × ‘Riparia Teleki 4B (СО4)’ rootstock - planting year - 2011, planting scheme 3 m×1 m). On both plots, the training system used was vertical three-wire trellis, vines shaped as AZOS-1, the trunk height 1.3 m. The paper demonstrates the relevance of the work on improvement of ‘Kokur Beliy’ cultivar with the help of clonal breeding on rootstock cultivar combination ‘Kokur Beliy’ rootstock ‘Kober 5 BB’: the plants demonstrated high relative productivity (0.92), quite large bunches (365 g), yield per bush made on average 4.74 kg/bush, very high shoot productivity as per raw bunch weight 336.6 g/shoot, a large percentage of pulp and juice output - 91.3 %. Assessment of the cultivar-rootstock combinations under production conditions allowed making individual selection of genotypically valuable, from the biological and economic points of view, plants.

scholarly journals Common Birds of Chitwan National Park, Nepal

2017 ◽  
Vol 1 (2) ◽  
pp. 19-26
Author(s):  
Parveen Kumar Jha
Keyword(s):  
Protected Area ◽  
National Park ◽  
Bird Species ◽  
Point Of View ◽  
Central Nepal ◽  
South Central ◽  
Tropical Vegetation ◽  
Chitwan National Park ◽  
Common Birds ◽  
Very High

 This research paper gives checklist of common birds of Chitwan National Park, which is a wild-life protected area in south-central Nepal. It covers tropical and sub-tropical vegetation. It is first protected area and includes 932 sq. km. Common birds observed are about 170 belonging to 48 Avian families during 2013-2014. Present investigator has very minutely observed birds in habitat conditions. Bird species were recognized by very high binocular. Birds were thoroughly studied from point of view of Taxonomy. Machans were also erected for observing birds.

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