scholarly journals Ultrastructural Changes In Renal Tubules of Sheep Following Experimental Infection With Leptospira Interrogans Serotype Pomona

1974 ◽  
Vol 7 (4) ◽  
pp. 505-508 ◽  
Author(s):  
R. B. MARSHALL
Keyword(s):  
Experimental Infection ◽  
Ultrastructural Changes ◽  
Leptospira Interrogans ◽  
Renal Tubules

Transcriptomics and Transmission Ultrastructural Examination Reveals the Nephrotoxicity of Cadmium in Laying Hens

2021 ◽  
Author(s):  
Man Zhao ◽  
Wenbo He ◽  
Can Tao ◽  
Beiyu Zhang ◽  
Shuai Wang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Basal Diet ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Renal Tubules ◽  
Rna Seq ◽  
Ultrastructural Examination ◽  
Treatment Groups ◽  
Kegg Pathway Analysis ◽  
Transcriptome Expression

Abstract The objective of this study was to reveal the effects of cadmium (Cd) on ultrastructural changes, oxidative stress, and transcriptome expression in the kidneys of laying hens. Seventy-two healthy Hy-Line brown laying hens at 41 weeks old were randomly allocated to four treatment groups with six replicates. The control group received a basal diet without additional Cd incorporation, and the other three treatment groups received diets supplemented with 15, 30, or 60 mg Cd /kg of feed. After 6 weeks of exposure the results show that administration of 60 mg/kg Cd significantly reduced (P < 0.05) eggshell thickness. With an increase in the Cd concentration in feed, the concentrations of renal Zn, Fe also had changed. Renal histopathology and ultrastructure also showed aggravated damage to glomeruli and renal tubules, and the deformation of nuclei and mitochondria in all Cd treatment groups. With an increase in Cd in feed, the activity of GPX and CAT was significantly reduced (P＜0.05), while the activity of T-AOC was decreased (P＜0.05) only in the 60 mg/kg Cd group. RNA-seq analysis revealed that 410 genes displayed differential expression (≥ 1.5-fold) in the 60 mg/kg supplementation group, compared to the control group. GO and KEGG pathway analysis results showed that Cd affected many genes involved in mitochondria and ion transport. In conclusion, this study elaborates the mechanisms underlying renal toxicity caused by Cd, which might provide target candidate genes for alleviating Cd poisoning in laying hens.

Changes in serum levels of purines in an experimental infection by Leptospira interrogans serovar Icterohaemorrhagiae

2014 ◽  
Vol 24 (4) ◽  
pp. 721-725
Author(s):  
Alexandre A. Tonin ◽  
Aleksandro S. Da Silva ◽  
Jeandre A. Jaques ◽  
Viviane C. G. Souza ◽  
Paula T. Tonin ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Serum Levels ◽  
Leptospira Interrogans

scholarly journals Oral Immunization with Escherichia coli Expressing a Lipidated Form of LigA Protects Hamsters against Challenge with Leptospira interrogans Serovar Copenhageni

2013 ◽  
Vol 82 (2) ◽  
pp. 893-902 ◽  
Author(s):  
Kristel Lourdault ◽  
Long-Chieh Wang ◽  
Ana Vieira ◽  
James Matsunaga ◽  
Rita Melo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Oral Vaccine ◽  
Oral Immunization ◽  
Reservoir Host ◽  
Leptospira Interrogans ◽  
Renal Tubules ◽  
Hamster Model ◽  
Content Type ◽  
E Coli ◽  
Humoral Immune

ABSTRACTLeptospirosis is a potentially fatal zoonosis transmitted by reservoir host animals that harbor leptospires in their renal tubules and shed the bacteria in their urine.Leptospira interrogansserovar Copenhageni transmitted fromRattus norvegicusto humans is the most prevalent cause of urban leptospirosis. We examinedL. interrogansLigA, domains 7 to 13 (LigA7-13), as an oral vaccine delivered byEscherichia colias a lipidated, membrane-associated protein. The efficacy of the vaccine was evaluated in a susceptible hamster model in terms of the humoral immune response and survival from leptospiral challenge. Four weeks of oral administration of liveE. coliexpressing LigA7-13 improved survival from intraperitoneal (i.p.) and intradermal (i.d.) challenge byL. interrogansserovar Copenhageni strain Fiocruz L1-130 in Golden Syrian hamsters. Immunization withE. coliexpressing LigA7-13 resulted in a systemic antibody response, and a significant LigA7-13 IgG level after the first 2 weeks of immunization was completely predictive of survival 28 days after challenge. As in previous LigA vaccine studies, all immunized hamsters that survived infection had renal leptospiral colonization and histopathological changes. In summary, an oral LigA-based vaccine improved survival from leptospiral challenge by either the i.p. or i.d. route.

scholarly journals Major Surface Protein LipL32 Is Not Required for Either Acute or Chronic Infection with Leptospira interrogans

2008 ◽  
Vol 77 (3) ◽  
pp. 952-958 ◽  
Author(s):  
Gerald L. Murray ◽  
Amporn Srikram ◽  
David E. Hoke ◽  
Elsio A. Wunder ◽  
Rebekah Henry ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Chronic Infection ◽  
Matrix Protein ◽  
Severe Disease ◽  
Extracellular Matrix Protein ◽  
Leptospira Interrogans ◽  
Renal Tubules ◽  
Wild Type

ABSTRACT Leptospira interrogans is responsible for leptospirosis, a zoonosis of worldwide distribution. LipL32 is the major outer membrane protein of pathogenic leptospires, accounting for up to 75% of total outer membrane protein. In recent times LipL32 has become the focus of intense study because of its surface location, dominance in the host immune response, and conservation among pathogenic species. In this study, an lipL32 mutant was constructed in L. interrogans using transposon mutagenesis. The lipL32 mutant had normal morphology and growth rate compared to the wild type and was equally adherent to extracellular matrix. Protein composition of the cell membranes was found to be largely unaffected by the loss of LipL32, with no obvious compensatory increase in other proteins. Microarray studies found no obvious stress response or upregulation of genes that may compensate for the loss of LipL32 but did suggest an association between LipL32 and the synthesis of heme and vitamin B12. When hamsters were inoculated by systemic and mucosal routes, the mutant caused acute severe disease manifestations that were indistinguishable from wild-type L. interrogans infection. In the rat model of chronic infection, the LipL32 mutant colonized the renal tubules as efficiently as the wild-type strain. In conclusion, this study showed that LipL32 does not play a role in either the acute or chronic models of infection. Considering the abundance and conservation of LipL32 among all pathogenic Leptospira spp. and its absence in saprophytic Leptospira, this finding is remarkable. The role of this protein in leptospiral biology and pathogenesis thus remains elusive.

scholarly journals Experimental infection of monkeys withLeptospira interrogansserovarhardjo

1987 ◽  
Vol 98 (2) ◽  
pp. 191-197 ◽  
Author(s):  
M. F. Palmer ◽  
Sheena A. Waitkins ◽  
R. B. Fitzgeorge ◽  
A. Baskerville
Keyword(s):  
Experimental Infection ◽  
Clinical Disease ◽  
Leptospira Interrogans ◽  
Route Of Infection ◽  
Immunofluorescent Technique ◽  
Different Strains

SUMMARYGrivet monkeys experimentally infected with two different strains ofLeptospira interrogansserovarhardjoshowed no signs of severe clinical disease. There were no significant macroscopic lesions in any of the tissues examined, but the organisms were demonstrated in various tissues by immunofluorescent technique and were isolated from the blood and urine of two monkeys and the kidney of one. Abraded skin was shown to be a viable route of infection in non-human primates.

scholarly journals Ultrastructural aspects of experimental cryptosporidiosis in pigs

1999 ◽  
Vol 51 (5) ◽  
pp. 441-444
Author(s):  
A.P.F.R.L. Bracarense ◽  
A.C.F. Reis ◽  
I.L. Sinhorini
Keyword(s):  
Intestinal Mucosa ◽  
Experimental Infection ◽  
Peyer's Patches ◽  
Ultrastructural Changes ◽  
Intestinal Cells ◽  
Lymphoid Tissues ◽  
Peyer’S Patches

The aim of this work was to determine ultrastructural changes on the intestinal mucosa and associated lymphoid tissues after an experimental infection with Cryptosporidium sp. Twelve piglets dosed orally with 1×10(6) oocysts were slaughtered on days 3, 6, 9 and 12 after inoculation. The ultrastructural lesions in the intestinal cells were irregular with thickened microvilli, cytoplasmic protrusions and vacuolation, swollen mitochondria, hypertrophic organelles and nuclei. The lymphocytes of the Peyer’s patches occasionally were mitotic with a larger number of lymphoblasts in the inoculated animals.

scholarly journals ULTRASTRUCTURAL CHANGES OF THE RENAL TUBULES FOLLOWING

1974 ◽  
Vol 65 (1) ◽  
pp. 6-17
Author(s):  
Tadashi Suzuki ◽  
Sadao Tsunematsu ◽  
Katsukuni Naruse ◽  
Tomihisa Funyu
Keyword(s):  
Ultrastructural Changes ◽  
Renal Tubules
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