scholarly journals Catellibacterium nectariphilum gen. nov., sp. nov., which requires a diffusible compound from a strain related to the genus Sphingomonas for vigorous growth

2004 ◽  
Vol 54 (3) ◽  
pp. 955-959 ◽  
Author(s):  
Yasuhiro Tanaka ◽  
Satoshi Hanada ◽  
Akira Manome ◽  
Takayasu Tsuchida ◽  
Ryuichiro Kurane ◽  
...  
Keyword(s):  
Optimal Growth ◽  
Cellular Fatty Acid ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Vigorous Growth ◽  
Major Respiratory Quinone ◽  
Ubiquinone 10 ◽  
Phototrophic Growth

A bacterial strain, designated AST4T, was isolated from activated sludge. The bacterium did not show significant growth on nutrient broth, but growth was clearly stimulated by addition of supernatant from other bacterial cultures. Culture filtrate of a strain related to the genus Sphingomonas in particular increased the cell yield and growth rate of strain AST4T. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain AST4T is located within the ‘Rhodobacter group’ in the α-3 subclass of Proteobacteria, but is clearly distant from related genera in this group such as Paracoccus, Rhodobacter and Rhodovulum. Strain AST4T is a Gram-negative, non-motile, rod-shaped (0·6–0·8×1·3–2·0 μm) and aerobic bacterium. It was not able to reduce nitrate to nitrite or N2. No phototrophic growth was observed. Optimal growth occurred at 30 °C and pH 6·5–7·5. The dominant cellular fatty acid in the isolate was C18 : 1 cis11. Ubiquinone-10 was the major respiratory quinone. The G+C content was 64·5 mol% (by HPLC). Based on the phylogenetic and phenotypic traits, the name Catellibacterium nectariphilum gen. nov., sp. nov. is proposed for this isolate; the type strain is AST4T (=NBRC 100046T=JCM 11959T=DSM 15620T).

scholarly journals Paracoccus isoporae sp. nov., isolated from the reef-building coral Isopora palifera

2011 ◽  
Vol 61 (5) ◽  
pp. 1138-1143 ◽  
Author(s):  
Ming-Hui Chen ◽  
Shih-Yi Sheu ◽  
Chaolun Allen Chen ◽  
Jih-Terng Wang ◽  
Wen-Ming Chen
Keyword(s):  
Phylogenetic Analyses ◽  
Optimal Growth ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Respiratory Quinone ◽  
Polar Lipid Profile ◽  
Major Respiratory Quinone ◽  
Taxonomic Approach ◽  
Isopora Palifera

A bacterial strain, designated SW-3T, was isolated from the reef-building coral Isopora palifera, from Southern Taiwan, and was characterized by using a polyphasic taxonomic approach. Cells of strain SW-3T were Gram-reaction-negative, aerobic, rod-shaped and motile by means of monopolar flagella and formed cream–white colonies. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-3T belonged to the genus Paracoccus and its most closely related neighbours were P. aestuarii B7T, P. homiensis DD-R11T, P. marinus KKL-A5T, P. denitrificans DSM 413T and P. zeaxanthinifaciens R-1512T, with sequence similarities of 96.8, 96.6, 96.1 , 95.8 and 94.9 %, respectively. Strain SW-3T exhibited optimal growth at 25–30 °C and pH 9–10 and in 3–5 % (w/v) NaCl. The predominant cellular fatty acid was summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c; 75 %). The major respiratory quinone was Q-10. The polar lipid profile consisted of a mixture of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and several uncharacterized polar lipids. The DNA G+C content of strain SW-3T was 63.7 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from recognized species of the genus Paracoccus. It is evident from the genotypic, phenotypic and chemotaxonomic data that strain SW-3T represents a novel species of the genus Paracoccus, for which the name Paracoccus isoporae sp. nov. is proposed. The type strain is SW-3T ( = LMG 25205T  = BCRC 17967T).

scholarly journals Catellibacterium nanjingense sp. nov., a propanil-degrading bacterium isolated from activated sludge, and emended description of the genus Catellibacterium

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 495-499 ◽  
Author(s):  
Jun Zhang ◽  
Shu-An Chen ◽  
Jin-Wei Zheng ◽  
Shu Cai ◽  
Bao-Jian Hang ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Membrane Structures ◽  
Respiratory Quinone ◽  
Degrading Bacterium ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Ubiquinone 10 ◽  
Sequence Similarities

A novel facultatively anaerobic, non-spore-forming, non-motile, catalase- and oxidase-positive, Gram-negative and rod-shaped bacterial strain, designated Y12T, was isolated from activated sludge of a wastewater bio-treatment facility. The strain was able to degrade about 90 % of added propanil (100 mg l−1) within 3 days of incubation. Growth occurred in the presence of 0–4.5 % (w/v) NaCl (optimum 0.5 %), at 10–40 °C (optimum 28 °C) and at pH 5.5–10.0 (optimum pH 7.0). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone-10 and the major cellular fatty acid was summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c). The genomic DNA G+C content of strain Y12T was 63.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain Y12T was a member of the genus Catellibacterium, as it showed highest sequence similarities to Catellibacterium caeni DCA-1T (99.1 %) and <96.0 % similarities with other species of the genus Catellibacterium. Strain Y12T showed low DNA–DNA relatedness values with C. caeni DCA-1T. Based on phenotypic, genotypic and phylogenetic properties, strain Y12T represents a novel species of the genus Catellibacterium, for which the name Catellibacterium nanjingense sp. nov. is proposed. The type strain is Y12T ( = CCTCC AB 2010218T  = KCTC 23298T). An emended description of the genus Catellibacterium is also presented.

Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter

2012 ◽  
Vol 62 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Xiuqing Xue ◽  
Kundi Zhang ◽  
Feng Cai ◽  
Jun Dai ◽  
Yang Wang ◽  
...  
Keyword(s):  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Desert Sand ◽  
Emended Description ◽  
Major Respiratory Quinone ◽  
Bacterium Strain ◽  
Ubiquinone 10 ◽  
Type Strains

A Gram-negative, rod-shaped, non-motile, strictly aerobic bacterium, strain S3-63T, was isolated from desert sand of Xinjiang, China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S3-63T had highest similarity to type strains of the genus Altererythrobacter, i.e. Altererythrobacter marinus H32T (97.2 % similarity), Altererythrobacter marensis MSW-14T (95.9 %), Altererythrobacter aestuarii KCTC 22735T (95.5 %), Altererythrobacter epoxidivorans JCS350T (95.1 %), Altererythrobacter namhicola KCTC 22736T (95.1 %), Altererythrobacter luteolus SW-109T (95.0 %) and Altererythrobacter indicus LMG 23789T (93.5 %). Growth occurred at 20–37 °C (optimum 30 °C), at pH 7.0–9.0 (optimum pH 8.0) and in 0–3 % (w/v) NaCl (optimum 1 %). The major respiratory quinone was ubiquinone-10 and the predominant cellular fatty acids were C18 : 1ω7c (50.8 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 12.6 %), C16 : 0 (12.3 %), C14 : 0 2-OH (7.3 %) and C17 : 1ω6c (4.5 %). The DNA G+C content was 64.6 mol%. Therefore, the phylogenetic, physiological and chemotaxonomic data demonstrated that strain S3-63T represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter xinjiangensis sp. nov. is proposed; the type strain is S3-63T ( = CCTCC AB 207166T = CIP 110125T). An emended description of the genus Altererythrobacter is provided.

scholarly journals Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment

2010 ◽  
Vol 60 (12) ◽  
pp. 2996-3001 ◽  
Author(s):  
Shi-Ping Tian ◽  
Yong-Xia Wang ◽  
Bin Hu ◽  
Xiao-Xia Zhang ◽  
Wei Xiao ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Soda Lake ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Sequence Comparisons ◽  
Respiratory Quinone ◽  
Major Respiratory Quinone ◽  
Respective Type

A novel alkaliphilic, halotolerant, rod-shaped bacterium, designated strain YIM CH208T, was isolated from a soda lake in Yunnan, south-west China. The taxonomy of strain YIM CH208T was investigated by a polyphasic approach. Strain YIM CH208T was Gram-negative, strictly aerobic and non-motile and formed red colonies. Optimal growth conditions were 28 °C, pH 8.5 and 0.5–2.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct line within a clade containing the genus Echinicola in the phylum Bacteroidetes and was related to the species Echinicola pacifica and Rhodonellum psychrophilum, with sequence similarity of 91.7 and 91.6 % to the respective type strains. The DNA G+C content was 45.1 mol%. The major respiratory quinone was menaquinone-7 (MK-7). The predominant cellular fatty acids were iso-C17 : 1 ω9c (19.9 %), C15 : 0 3-OH (12.1 %), iso-C17 : 0 3-OH (11.3 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 10.7 %) and C17 : 1 ω6c (8.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain YIM CH208T represents a novel species of a new genus, for which the name Litoribacter ruber gen. nov., sp. nov. is proposed. The type strain of Litoribacter ruber is YIM CH208T (=ACCC 05414T =KCTC 22899T).

scholarly journals Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2805-2811 ◽  
Author(s):  
Zhang Zhen-Li ◽  
Zhang Xin-Qi ◽  
Wu Nan ◽  
Zhang Wen-Wu ◽  
Zhu Xu-Fen ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
Major Respiratory Quinone

A thermotolerant, Gram-strain-negative, non-spore-forming and strictly aerobic bacterium, designated GU51T, was isolated from Guhai hot spring in Jimsar county, Xinjiang province, north-west China. Each cell of strain GU51T consisted of an oval body and two symmetrical long (3–6 µm) prosthecae. The strain moved by polar flagellum. Oxidase and catalase were produced. Strain GU51T grew within the ranges of 37–65 °C (optimum 48–50 °C), 0.5–7.5 % (w/v) NaCl (optimum 2–3 %) and pH 6.0–9.0 (optimum pH 7.5). The major respiratory quinone detected was ubiquinone 10 (U-10) and the genomic DNA G+C content was 66.7±0.4 mol%. Major fatty acids (>5 %) were C16 : 0, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, five glycolipids, phosphatidylglycerol and an unknown phospholipid. Phylogenetic analysis showed the closest relatives of strain GU51T were members of the genus Parvularcula with 92.3 % 16S rRNA gene sequence similarity. On the basis of this polyphasic taxonomic characterization, it is suggested that strain GU51T represents a novel species of a new genus in the family ‘ Parvularculaceae ’, for which the name Amphiplicatus metriothermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is GU51T ( = CGMCC 1.12710T = JCM 19779T).

scholarly journals Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome

2007 ◽  
Vol 57 (5) ◽  
pp. 1050-1054 ◽  
Author(s):  
Seung Seob Bae ◽  
Kae Kyoung Kwon ◽  
Sung Hyun Yang ◽  
Hee-Soon Lee ◽  
Sang-Jin Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Bacterium ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Respiratory Quinone ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Major Respiratory Quinone

A marine bacterium, DOKDO 007T, was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007T were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca2+ ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1ω10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007T should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007T (=KCCM 42307T=JCM 13831T).

scholarly journals Nonlabens ponticola sp. nov., isolated from seawater and reclassification of Nonlabens sediminis as a later heterotypic synonym of Nonlabens tegetincola

2020 ◽  
Author(s):  
Ye Lin Seo ◽  
Jaejoon Jung ◽  
Chi-une Song ◽  
Yong Min Kwon ◽  
Hye Su Jung ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
16S Rrna Sequence ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone

A Gram-stain-negative, orange-pigmented and strictly aerobic bacterium, designated strain MJ115T, was isolated from seawater in Pohang, South Korea. Cells were non-motile rods and showed positive reactions for catalase and oxidase tests. Growth of strain MJ115T was observed at 4–35 °C (optimum, 30 °C), pH 6.0–7.0 (optimum, pH 6.5) and in the presence of 0–8.0 % (w/v) NaCl (optimum, 2.0%). Strain MJ115T contained iso-C15 : 0, anteiso-C15 : 0, anteiso-C17 : 1  ω9c, C17 : 0 2-OH, iso-C16 : 0 3-OH, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c) as major cellular fatty acids and menaquinone-6 as the major respiratory quinone. Phosphatidylethanolamine, two unidentified aminolipids and four unidentified lipids were detected as major polar lipids. The G+C content of the genomic DNA was 40.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain MJ115T formed a phyletic lineage with Nonlabens marinus S1-08T, Nonlabens agnitus JC2678T and Nonlabens antarcticus AKS 622T within the genus Nonlabens . Strain MJ115T was also most closely related to N. marinus S1-08T, N. agnitus JC2678T and N. antarcticus AKS 622T with 96.5, 96.4 and 96.0 % 16S rRNA sequence similarities, respectively. Here it is proposed that strain MJ115T represents a new species of the genus Nonlabens , for which the name Nonlabens ponticola sp. nov. is proposed. The type strain is MJ115T (=KCTC 72237T=NBRC 113963T). In addition, the comparison of the whole genome sequences and phenotypic features suggested that Nonlabens tegetincola and Nonlabens sediminis belong to the same species. Therefore, it is proposed that N. sediminis is reclassified as a later heterotypic synonym of N. tegetincola .

scholarly journals Gillisia myxillae sp. nov., a novel member of the family Flavobacteriaceae, isolated from the marine sponge Myxilla incrustans

2006 ◽  
Vol 56 (8) ◽  
pp. 1795-1799 ◽  
Author(s):  
On On Lee ◽  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Marine Sponge ◽  
Phenotypic Traits ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Total Fatty Acids ◽  
Bacterium Strain

A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085T) was isolated from the surface of a marine sponge, Myxilla incrustans, at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17 : 0 3-OH, 17 : 0 2-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 1ω7c (altogether representing 69.0 % of the total fatty acids). MK-6 was the only respiratory quinone detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of UST050418-085T were members of the genus Gillisia, with sequence similarities of 93.2–96.6 %. Strain UST050418-085T differed from its closest relatives by 11 to 18 phenotypic traits. Molecular evidence and phenotypic characteristics suggest that strain UST050418-085T represents a novel species within the genus Gillisia. The name Gillisia myxillae sp. nov. is proposed, with UST050418-085T (=JCM 13546T=NRRL B-41416T) as the type strain.

scholarly journals Salirhabdus euzebyi gen. nov., sp. nov., a Gram-positive, halotolerant bacterium isolated from a sea salt evaporation pond

2007 ◽  
Vol 57 (7) ◽  
pp. 1566-1571 ◽  
Author(s):  
Luciana Albuquerque ◽  
Igor Tiago ◽  
Fred A. Rainey ◽  
Marco Taborda ◽  
M. Fernanda Nobre ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Optimal Growth ◽  
Sea Salt ◽  
Rrna Gene ◽  
Gram Positive ◽  
Respiratory Quinone ◽  
Evaporation Pond ◽  
Optimum Growth Temperature ◽  
Major Respiratory Quinone

A low-G+C, Gram-positive bacterium, designated CVS-14T, was recovered from a sea salt evaporation pond on the island of Sal in the Cape Verde Archipelago. This organism was catalase- and oxidase-positive. Cells were motile, spore-forming aerobic rods, with an optimum growth temperature of about 35–40 °C and optimum pH between 7.0 and 8.5. Optimal growth occurred in media containing 4–6 % (w/v) NaCl, although the organism was able to grow in medium without added NaCl and in medium containing 16 % NaCl. The cell-wall peptidoglycan was of A1γ type and the major respiratory quinone was menaquinone 7 (MK-7). Major fatty acids were iso-15 : 0, anteiso-15 : 0, iso-17 : 0 and anteiso-17 : 0. The DNA G+C content was 37.0 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain CVS-14T formed a distinct new branch within the radiation of the moderately halophilic bacilli group, forming a separate lineage from species of the genera Salinibacillus, Paucisalibacillus, Oceanobacillus, Lentibacillus and Virgibacillus. Strain CVS-14T showed 16S rRNA gene pairwise similarity values of ~95 % with species of the genus Salinibacillus. On the basis of morphological, physiological, chemotaxonomic and phylogenetic characteristics, strain CVS-14T is considered to represent a novel species in a new genus, for which the name Salirhabdus euzebyi gen. nov., sp. nov. is proposed. The type strain is CVS-14T (=LMG 22839T=CIP 108577T).

Amorphus suaedae sp. nov., isolated from the root of a tidal flat plant, Suaeda maritima

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3868-3872 ◽  
Author(s):  
Jung Moon Hwang ◽  
Eu Jin Chung ◽  
Jeong Ae Park ◽  
Jae Heon Jeong ◽  
Che Ok Jeon ◽  
...  
Keyword(s):  
Tidal Flat ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone ◽  
Ubiquinone 10

A novel bacterial strain, YC6899T, was isolated from the root of Suaedae maritima growing on a tidal flat of Namhae Island, Korea. Cells were Gram-reaction-negative, rod-shaped, non-motile, slightly halophilic and heterotrophic. Strain YC6899T grew optimally at a salinity of 2–4 %, at 25–37 °C and at pH 6.5–8.0. Phylogenetic analysis of 16S rRNA gene sequences demonstrated that strain YC6899T was closely related to Amorphus orientalis YIM D10T (96.1 % similarity) and Amorphus coralli RS.Sph.026T (95.9 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unknown aminolipids, an unknown aminophospholipid, an unknown aminoglycolipid, unknown glycolipids and unknown lipids. The major fatty acids of strain YC6899T were C19 : 0 cyclo ω8c and C18 : 1ω7c. The G+C content of the genomic DNA was 61.3 mol%. Strain YC6899T contained ubiquinone-10 (Q-10) as the major respiratory quinone system. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain YC6899T represents a novel species within the genus Amorphus , for which the name Amorphus suaedae sp. nov. is proposed. The type strain is YC6899T ( = KACC 14912T = NBRC 107845T).

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