scholarly journals Hoeflea phototrophica sp. nov., a novel marine aerobic alphaproteobacterium that forms bacteriochlorophyll a

2006 ◽  
Vol 56 (4) ◽  
pp. 821-826 ◽  
Author(s):  
Hanno Biebl ◽  
Brian J. Tindall ◽  
Rüdiger Pukall ◽  
Heinrich Lünsdorf ◽  
Martin Allgaier ◽  
...  
Keyword(s):  
Sea Water ◽  
Sequence Data ◽  
Sequence Similarity ◽  
Photosynthetic Pigment ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Good Growth ◽  
Reaction Centre ◽  
The Novel ◽  
Poor Growth

Within a collection of marine strains that were shown to contain the photosynthesis reaction-centre genes pufL and pufM, a novel group of alphaproteobacteria was found and was characterized phenotypically. The 16S rRNA gene sequence data suggested that the strains belonged to the order Rhizobiales and were closest (98·5 % sequence similarity) to the recently described species Hoeflea marina. The cells contained bacteriochlorophyll a and a carotenoid, presumably spheroidenone, in small to medium amounts. Cells of the novel strains were small rods and were motile by means of single polarly inserted flagella. Good growth occurred in complex media with 0·5–7·0 % sea salts, at 25–33 °C (optimum, 31 °C) and at pH values in the range 6–9. With the exception of acetate and malate, organic carbon sources tested supported poor growth or no growth at all. Growth factors were required; these were provided by small amounts of yeast extract, but not by standard vitamin solutions. Growth occurred under aerobic to microaerobic conditions, but not under anaerobic conditions, either in the dark or light. Nitrate was not reduced. Photosynthetic pigments were formed at low to medium salt concentrations, but not at the salt concentration of sea water (3·5 %). On the basis of smaller cell size, different substrate utilization profile and photosynthetic pigment content, the novel strains can be classified as representatives of a second species of Hoeflea, for which the name Hoeflea phototrophica sp. nov. is proposed. The type strain of Hoeflea phototrophica sp. nov. is DFL-43T (=DSM 17068T=NCIMB 14078T).

scholarly journals Nereida ignava gen. nov., sp. nov., a novel aerobic marine α-proteobacterium that is closely related to uncultured Prionitis (alga) gall symbionts

2005 ◽  
Vol 55 (2) ◽  
pp. 631-636 ◽  
Author(s):  
M. J. Pujalte ◽  
M. C. Macián ◽  
D. R. Arahal ◽  
W. Ludwig ◽  
K. H. Schleifer ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Sea Water ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Good Growth ◽  
Strictly Aerobic ◽  
Phylogenetic Distance ◽  
Novel Genus And Species

A Gram-negative, slightly halophilic, non-pigmented, strictly aerobic, chemo-organotrophic bacterium was isolated from Mediterranean sea water off the Spanish coast near Valencia. This strain was poorly reactive, being unable to grow in most carbon sources analysed in minimal medium. However, good growth was observed when more complex media and longer incubation times were used. Phylogenetic analysis based on an almost complete 16S rRNA gene sequence placed strain 2SM4T within the Roseobacter group, in the vicinity of uncultured bacteria described as gall symbionts of several species of the red alga Prionitis. Sequence similarity values between strain 2SM4T and the closest neighbouring species were below 95·0 %. The cellular fatty acid composition of the Mediterranean strain confirmed its position within the ‘Alphaproteobacteria’, sharing 18 : 1ω7c as the major cellular fatty acid. The phylogenetic distance from any taxon with a validly published name and also a number of distinguishing features support the designation of strain 2SM4T as representing a novel genus and species, for which the name Nereida ignava gen. nov., sp. nov. is proposed. The type strain is 2SM4T (=CECT 5292T=DSM 16309T=CIP 108404T=CCUG 49433T).

scholarly journals Kordia algicida gen. nov., sp. nov., an algicidal bacterium isolated from red tide

2004 ◽  
Vol 54 (3) ◽  
pp. 675-680 ◽  
Author(s):  
Jae Hak Sohn ◽  
Jung-Hyun Lee ◽  
Hana Yi ◽  
Jongsik Chun ◽  
Kyung Sook Bae ◽  
...  
Keyword(s):  
Sea Water ◽  
Sequence Similarity ◽  
Red Tide ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Characters ◽  
Algicidal Bacterium ◽  
Rdna Sequences ◽  
The Family

A bacterium (named OT-1T) that showed algicidal activity was isolated from sea water of Masan Bay, Korea, during an outbreak of red tide. Phylogenetic analysis based on 16S rDNA sequences showed that the isolate formed a distinct phyletic lineage within the family Flavobacteriaceae of the Cytophaga–Flavobacterium–Bacteroides group. No species with a validly published name showed ⩾93 % 16S rRNA gene sequence similarity to strain OT-1T. The isolate had major amounts of iso-branched and 3-hydroxy iso-branched fatty acids and menaquinone 6 and a DNA G+C content of 34 mol%; these chemotaxonomic characters also supported the placement of the organism in the family Flavobacteriaceae. The strain was Gram-negative, yellow-pigmented, non-motile, non-gliding, flexirubin-negative, strictly aerobic, catalase-negative, oxidase-positive and halophilic. Na+, Ca2+ and Mg2+ ions were obligately required for growth. The strain utilized various sugars as sole carbon sources and degraded gelatin, skimmed milk and starch. Several phenotypic characters can be used to differentiate the test strain from phylogenetically related marine bacterial genera. On the basis of polyphasic evidence, it is proposed that strain OT-1T should be assigned to the family Flavobacteriaceae as Kordia algicida gen. nov., sp. nov. The type strain is OT-1T (=KCTC 8814PT=NBRC 1000336T).

scholarly journals Tamilnaduibacter salinus gen. nov., sp. nov., a halotolerant gammaproteobacterium within the family Alteromonadaceae, isolated from a salt pan in Tamilnadu, India

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3248-3255 ◽  
Author(s):  
Ashish Verma ◽  
Poonam Mual ◽  
Shanmugam Mayilraj ◽  
Srinivasan Krishnamurthi
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Carbon Sources ◽  
Rrna Gene ◽  
The Novel ◽  
The Family ◽  
Molecular Phylogenetic ◽  
Salt Pan ◽  
Slow Growing

Two novel Gram-stain-negative, slow-growing, halotolerant strains with rod-shaped cells, designated as strains Mi-7T and Mi-8, which formed pin-point colonies on halophilic media were isolated during a study into the microbial diversity of a salt pan in the state of Tamilnadu, India. Both the strains had an obligate requirement for 1 % (w/v) NaCl for growth and were halotolerant, growing at NaCl concentrations of up to 20 % (w/v) in media. The strains, however, showed an inability to utilize the majority of substrates tested as sole carbon sources for growth and in fermentation reactions. Molecular phylogenetic analyses, based on 16S rRNA gene sequence revealed their closest phylogenetic neighbours to be members of the genus Marinobacter, with whom they showed the highest sequence similarity of 93.6 % and even less with the type strain of the type species, Marinobacter hydrocarbonoclasticus DSM 8798T (91.1 %). Similarities with other genera within the family Alteromonadaceae were below 91.0 %. However, the two strains were very closely related to each other with 99.9 % sequence similarity, and DNA–DNA hybridization analyses confirmed their placement in the same species. The DNA G+C content of both strains was 65 mol%. Using the polyphasic taxonomic data obtained from this study, strains Mi-7T and Mi-8 represent two strains of the same species of a novel genus for which the name Tamilnaduibacter salinus gen. nov., sp. nov., is proposed; the type strain of the novel species is Mi-7T ( = MTCC 12009T = DSM 28688T).

scholarly journals Loktanella agnita sp. nov. and Loktanella rosea sp. nov., from the north-west Pacific Ocean

2005 ◽  
Vol 55 (5) ◽  
pp. 2203-2207 ◽  
Author(s):  
Elena P. Ivanova ◽  
Natalia V. Zhukova ◽  
Anatoly M. Lysenko ◽  
Nataliya M. Gorshkova ◽  
Alexander F. Sergeev ◽  
...  
Keyword(s):  
Pacific Ocean ◽  
Sea Water ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Tween 80 ◽  
Rrna Gene ◽  
North West ◽  
Temperature Range ◽  
West Pacific Ocean ◽  
The North

One whitish and four pinkish strains of Gram-negative, non-motile, aerobic bacteria were isolated from sea-water and sediment samples collected in Chazhma Bay (Sea of Japan, Pacific Ocean). Analysis of 16S rRNA gene sequences revealed that these strains belonged to the ‘Alphaproteobacteria’, having highest sequence similarity of about 94–97 % with species of the genus Loktanella. None of the strains degraded gelatin, casein, chitin, agar, DNA or starch and they had limited ability to utilize carbon sources. The four pinkish strains, Fg36T, Fg1, Fg116 and Fg117, degraded Tween 80. Sea-water strain R10SW5T grew at 3–6 % NaCl and a temperature range of 8–35 °C, whilst strains Fg36T, Fg1, Fg116 and Fg117 grew at NaCl concentrations of 1–12 % and a temperature range of 4–35 °C. Phosphatidylglycerol (58/79 %), diphosphatidylglycerol (11/6 %) and phosphatidylcholine (28/22 %) were the major phospholipids. The predominant fatty acids were 16 : 0 (12·2/8·6 %) and 18 : 1ω7 (76·6/68·4 %). The DNA G+C content of strain R10SW5T was 59·1 mol% and those of the four pinkish strains ranged from 60·5 to 61·8 mol%. Based on the results of phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Loktanella agnita sp. nov. and Loktanella rosea sp. nov., are proposed. The type strains are R10SW5T (=KMM 3788T=CIP 107883T) and Fg36T (=KMM 6003T=CIP 107851T=LMG 22534T), respectively.

scholarly journals Enrichment and Physiological Characterization of a Novel Nitrospira-Like Bacterium Obtained from a Marine Sponge

2010 ◽  
Vol 76 (14) ◽  
pp. 4640-4646 ◽  
Author(s):  
Sandra Off ◽  
Mashal Alawi ◽  
Eva Spieck
Keyword(s):  
Marine Sponge ◽  
Sea Water ◽  
Enrichment Culture ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
Physiological Characterization ◽  
Inhibition Of Growth ◽  
Nitrite Oxidizing Bacteria ◽  
Aplysina Aerophoba

ABSTRACT Members of the nitrite-oxidizing genus Nitrospira are most likely responsible for the second step of nitrification, the conversion of nitrite (NO2 −) to nitrate (NO3 −), within various sponges. We succeeded in obtaining an enrichment culture of Nitrospira derived from the mesohyl of the marine sponge Aplysina aerophoba using a traditional cultivation approach. Electron microscopy gave first evidence of the shape and ultrastructure of this novel marine Nitrospira-like bacterium (culture Aa01). We characterized these bacteria physiologically with regard to optimal incubation conditions, especially the temperature and substrate range in comparison to other Nitrospira cultures. Best growth was obtained at temperatures between 28°C and 30°C in mineral medium with 70% North Sea water and a substrate concentration of 0.5 mM nitrite under microaerophilic conditions. The Nitrospira culture Aa01 is very sensitive against nitrite, because concentrations higher than 1.5 mM resulted in a complete inhibition of growth. Sequence analyses of the 16S rRNA gene revealed that the novel Nitrospira-like bacterium is separated from the sponge-specific subcluster and falls together with an environmental clone from Mediterranean sediments (98.6% similarity). The next taxonomically described species Nitrospira marina is only distantly related, with 94.6% sequence similarity, and therefore the culture Aa01 represents a novel species of nitrite-oxidizing bacteria.

scholarly journals Salegentibacter flavus sp. nov.

2006 ◽  
Vol 56 (3) ◽  
pp. 583-586 ◽  
Author(s):  
Elena P. Ivanova ◽  
John P. Bowman ◽  
Richard Christen ◽  
Natalia V. Zhukova ◽  
Anatoly M. Lysenko ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Sequence Data ◽  
Sequence Similarity ◽  
Tween 80 ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Straight Chain ◽  
Branched Chain

A yellow-pigmented, non-motile, Gram-negative bacterium, designated Fg 69T, was isolated from a sediment sample collected in Chazhma Bay (Sea of Japan). The novel organism grew at 10–35 °C, was neutrophilic and required 3–10 % NaCl for optimal growth. Strain Fg 69T was able to degrade starch and to hydrolyse gelatin and Tween 80 weakly but not casein or agar. Predominant cellular fatty acids comprised n-C15 and n-C16 branched-chain and straight-chain saturated and unsaturated fatty acids, including iso-C15 : 0 (5 %), anteiso-C15 : 0 (11 %), C15 : 0 (9 %), iso-C15 : 1 (5 %), iso-C16 : 0 (8 %), C16 : 0 (5 %) and C16 : 1 ω7 (5 %) and iso- and anteiso-branched 2-OH and 3-OH C15 : 0 to C17 : 0 fatty acids (26 % in total). The G+C content of the DNA was 40·4 mol%. 16S rRNA gene sequence data indicated that strain Fg 69T belonged to the genus Salegentibacter but was distinct from recognized Salegentibacter species (94–95 % sequence similarity). Based on these results, a novel species, Salegentibacter flavus sp. nov., is proposed. The type strain is Fg 69T (=KMM 6000T=CIP 107843T).

Weissella oryzae sp. nov., isolated from fermented rice grains

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1417-1420 ◽  
Author(s):  
Masanori Tohno ◽  
Maki Kitahara ◽  
Hidehiko Inoue ◽  
Ryuichi Uegaki ◽  
Tomohiro Irisawa ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Data ◽  
Sequence Similarity ◽  
Molar Ratio ◽  
Rrna Gene ◽  
The Novel ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Low Levels

A taxonomic study was conducted on two Gram-reaction-positive, catalase-negative, irregular short-rod-shaped or coccoid lactic acid bacteria, designated strains SG25T and SG23, that were isolated from grains of fermented Japanese rice (Oryza sativa L. subsp. japonica). A phylogenetic analysis based on 16S rRNA gene sequence data clearly showed that the strains belonged to the genus Weissella and were most closely related to Weissella soli LMG 20113T (with a sequence similarity of 96.9 % for each novel strain). The peptidoglycan of each strain contained the amino acids glutamic acid, lysine, serine and alanine in a molar ratio of 1.0 : 1.2 : 0.5 : 3.0, respectively. On the basis of the unusual phenotypic characteristics of the novel strains and the low levels of DNA–DNA relatedness recorded between each novel strain and Weissella soli JCM 12536T, strains SG25T and SG23 represent a single novel species in the genus Weissella , for which the name Weissella oryzae sp. nov. is proposed. The type strain is SG25T ( = JCM 18191T  = DSM 25784T).

scholarly journals Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China

2007 ◽  
Vol 57 (11) ◽  
pp. 2572-2577 ◽  
Author(s):  
Zong-Yun Hu ◽  
Yun Li
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Bacterium ◽  
Sequence Similarity ◽  
Coastal Sediments ◽  
Rrna Gene ◽  
Good Growth ◽  
The Novel ◽  
Pr China ◽  
The 16S Rrna Gene

A strain of heterotrophic, facultatively anaerobic, marine bacterium, designated strain c121T, was isolated from coastal sediment of Luoyuan Bay, in Fujian province, PR China. Analysis of the 16S rRNA gene sequence revealed an affiliation with the genus Pseudidiomarina; the sequence similarity between c121T and Pseudidiomarina taiwanensis PIT1T was 97 %. Cells of the novel strain were non-pigmented, Gram-negative rods, 0.3 μm wide and 1.2–1.8 μm long. Cells grown in broth cultures were non-motile, lacking flagella. Growth of the strain was observed at salinities ranging from 0.5 to 15 % NaCl, and the optimal concentration was about 1–8 %. The temperature range for growth was rather broad and was high for a marine bacterium: the strain grew at 13–42 °C, showed good growth at 20–40 °C and had an optimum between 30 and 40 °C. The major cellular fatty acids were iso-C15 : 0 (24.2 %), C16 : 1 ω7c/iso-C15 : 0 2-OH (15.3 %) and iso-C17 : 1 ω9c (11.9 %). The DNA G+C content was 50.0 mol%. Phylogeny based on 16S rRNA gene sequences, together with data from phenotypic and chemotaxonomic characterization, revealed that strain c121T could be classified within a novel species of the genus Pseudidiomarina, for which the name Pseudidiomarina sediminum sp. nov. is proposed, with the type strain c121T (=CICC 10319T =LMG 24046T).

scholarly journals Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 781-786 ◽  
Author(s):  
Maximo Sánchez ◽  
Martha-Helena Ramírez-Bahena ◽  
Alvaro Peix ◽  
María J. Lorite ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Lotus Corniculatus ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).

scholarly journals Umboniibacter marinipuniceus gen. nov., sp. nov., a marine gammaproteobacterium isolated from the mollusc Umbonium costatum from the Sea of Japan

2010 ◽  
Vol 60 (3) ◽  
pp. 603-609 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Naoto Tanaka ◽  
Galina M. Frolova
Keyword(s):  
Sea Of Japan ◽  
Sequence Similarity ◽  
Fatty Acid Analysis ◽  
Phenotypic Characterization ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Phylogenetic Distance ◽  
The Novel ◽  
Bacterial Strains ◽  
Separate Branch

Two bacterial strains, KMM 3891T and KMM 3892, were isolated from internal tissues of the marine mollusc Umbonium costatum collected from the Sea of Japan. The novel isolates were Gram-negative, aerobic, faint pink–reddish-pigmented, rod-shaped, non-motile, stenohaline and psychrotolerant bacteria that were unable to degrade most tested complex polysaccharides. Polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Fatty acid analysis revealed C17 : 1 ω6c, C17 : 0, C16 : 0 and C16 : 1 ω7c as the dominant components. The major isoprenoid quinone was Q-7. The DNA G+C content of strain KMM 3891T was 51.7 mol%. According to phylogenetic analysis of 16S rRNA gene sequences, strains KMM 3891T and KMM 3892 were positioned within the Gammaproteobacteria as a separate branch, sharing <93 % sequence similarity to their phylogenetic relatives including Saccharophagus degradans, Microbulbifer species, Endozoicomonas elysicola, Simiduia agarivorans and Teredinibacter turnerae. Based on phenotypic characterization and phylogenetic distance, the novel marine isolates KMM 3891T and KMM 3892 represent a new genus and species, for which the name Umboniibacter marinipuniceus gen. nov., sp. nov. is proposed. The type strain of Umboniibacter marinipuniceus is KMM 3891T (=NRIC 0753T =JCM 15738T).

Sign in / Sign up

Export Citation Format

Share Document