scholarly journals Howardella ureilytica gen. nov., sp. nov., a Gram-positive, coccoid-shaped bacterium from a sheep rumen

2007 ◽  
Vol 57 (12) ◽  
pp. 2940-2945 ◽  
Author(s):  
Anthony R. Cook ◽  
Paul W. Riley ◽  
Heather Murdoch ◽  
Paul N. Evans ◽  
Ian R. McDonald
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Rumen Fluid ◽  
Sequence Divergence ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Gram Positive ◽  
Gene Sequence Analysis ◽  
Sheep Rumen ◽  
Hydrolysis Of

An unidentified obligately anaerobic, fastidious, Gram-positive, non-motile, non-spore-forming, non-fermentative coccoid-shaped bacterium (designated strain GPC 589T) was isolated from the rumen fluid of a sheep. The major fatty acid constituents (>5 %) were C16 : 0 (29.2 %), C18 : 0 (40.7 %) and an unidentified compound (19.7 %) with an equivalent chain-length of 13.523. The G+C content of the DNA was 34 mol%. The organism was strongly ureolytic and generated ATP through the hydrolysis of urea. Comparative 16S rRNA gene sequence analysis demonstrated that strain GPC 589T was far removed, phylogenetically, from the ruminococci and related Gram-positive anaerobic cocci but exhibited a phylogenetic association with Clostridium rRNA cluster XIVa [as defined by Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. (1994). Int J Syst Bacteriol 44, 812–826]. Sequence divergence values of 12.5 % or more were observed between strain GPC 589T and all other recognized species within this and related rRNA clostridial clusters. Phylogenetic analysis showed that strain GPC 589T represents a new genus within cluster XIVa. On the basis of both phylogenetic and phenotypic evidence, it is proposed that strain GPC 589T should be classified as representing a new genus and novel species, Howardella ureilytica gen. nov., sp. nov. The type strain is strain GPC 589T (=DSM 15118T=JCM 13267T).

scholarly journals Winogradskyella thalassocola gen. nov., sp. nov., Winogradskyella epiphytica sp. nov. and Winogradskyella eximia sp. nov., marine bacteria of the family Flavobacteriaceae

2005 ◽  
Vol 55 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Suk Kyun Han ◽  
Cindy Snauwaert ◽  
Marc Vancanneyt ◽  
...  
Keyword(s):  
Type Strain ◽  
New Genus ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Respiratory Quinone ◽  
Peter The Great ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Major Respiratory Quinone

Three novel heterotrophic, Gram-negative, yellow-pigmented, aerobic, gliding, oxidase- and catalase-positive bacteria were isolated from algae collected in the Gulf of Peter the Great, Sea of Japan. 16S rRNA gene sequence analysis revealed that the strains studied represented members of the family Flavobacteriaceae and showed 93·5–93·8 % similarity with their closest relative, Psychroserpens burtonensis. The DNA G+C content of the strains was 34–37 mol%. The major respiratory quinone was MK-6. The predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1, iso-C16 : 0-3OH and iso-C17 : 0-3OH. On the basis of their phenotypic, chemotaxonomic, genotypic and phylogenetic characteristics, the newly described bacteria have been assigned to the new genus Winogradskyella gen. nov., as Winogradskyella thalassocola sp. nov. (type strain, KMM 3907T=KCTC 12221T=LMG 22492T=DSM 15363T), Winogradskyella epiphytica sp. nov. (type strain, KMM 3906T=KCTC 12220T=LMG 22491T=CCUG 47091T) and Winogradskyella eximia sp. nov. (type strain, KMM 3944T (=KCTC 12219T=LMG 22474T).

Nibribacter koreensis gen. nov., sp. nov., isolated from estuarine water

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4663-4668 ◽  
Author(s):  
Ji Young Kang ◽  
Jeesun Chun ◽  
Kwang Yeop Jahng
Keyword(s):  
Type Species ◽  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
Polar Lipid Profile ◽  
Content Type ◽  
Link Type ◽  
Gene Sequence Analysis

A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterium, designated strain GSR3061T, was isolated from a water sample of the Mangyung estuary enclosed by the Saemangeum Embankment in JEOLlabuk-do of South Korea, and characterized using a polyphasic approach. 16S rRNA gene sequence analysis of strain GSR3061T indicated that the isolate belonged to the phylum Bacteroidetes and exhibited similarity levels of 94.7 % to Rufibacter tibetensis NRRL B-51285T, 92.4 % to Adhaeribacter terreus KACC 14257T and 91.9 % to Pontibacter korlensis KACC 15371T. Growth was observed at 15–40 °C and pH 6.5–9.5. The major cellular fatty acids of the novel strain were summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0, C17 : 1ω6c and iso-C16 : 1 H. Flexirubin-type pigments were absent. The DNA G+C content of strain GSR3061T was 44.9 mol% and the major quinone was MK-7. The polar lipid profile consisted mainly of phosphatidylethanolamine; three unidentified lipids, two unknown aminolipids, two unknown phospholipids, an unknown aminophospholipid and an unknown glycolipid were also present. On the basis of the evidence presented, it is concluded that strain GSR3061T represents a novel species of a new genus, for which the name Nibribacter koreensis gen. nov., sp. nov. is proposed. The type strain of Nibribacter koreensis is GSR3061T ( = KACC 16450T = JCM 17917T).

scholarly journals Laetantesicola Maris Gen. Nov., Sp. Nov. Isolated From the Sea of Japan Shallow Sediments.

2021 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Valeriya V. Kurilenko ◽  
Nadezhda Yu. Chernysheva ◽  
Liudmila A. Tekutyeva ◽  
Peter V. Velansky ◽  
...  
Keyword(s):  
Sea Of Japan ◽  
Gene Sequence ◽  
Major Fatty Acid ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Novel Genus And Species ◽  
Distinct Lineage

Abstract A Gram-negative, non-motile bacterium КММ 3653T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КММ 3653T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.7%) and Nioella (95.1-94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КММ 3653T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to Vannielia litorea KCTC 32083T. The average nucleotide identity values between strain КММ 3653T and Vannielia litorea KCTC 32083T, Nioella nitratireducens KCTC 32417T, Litoreibacter albidus KMM 3851T, and Pseudoruegeria aquimaris CECT 7680T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КММ 3653T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid followed by C16:0. The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8 % was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, strain КММ 3653T is proposed to be classified as a novel genus and species Laetantesicola maris gen. nov., sp. nov. The type strain of Laetantesicola maris gen. nov., sp. nov. is КММ 3653T (=KCTC 82575T).

scholarly journals Azospirillum canadense sp. nov., a nitrogen-fixing bacterium isolated from corn rhizosphere

2007 ◽  
Vol 57 (3) ◽  
pp. 620-624 ◽  
Author(s):  
Samina Mehnaz ◽  
Brian Weselowski ◽  
George Lazarovits
Keyword(s):  
16S Rrna ◽  
Gene Sequence ◽  
Morphological Characterization ◽  
Major Fatty Acid ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Nitrogen Fixing ◽  
Closely Related Species ◽  
Gene Sequence Analysis ◽  
Biolog Analysis

A free-living diazotrophic strain, DS2T, was isolated from corn rhizosphere. Polyphasic taxonomy was performed including morphological characterization, Biolog analysis, and 16S rRNA, cpn60 and nifH gene sequence analyses. 16S rRNA gene sequence analysis indicated that strain DS2T was closely related to the genus Azospirillum (96 % similarity). Chemotaxonomic characteristics (DNA G+C content 67.9 mol%; Q-10 quinone system; major fatty acid 18 : 1ω7c) were also similar to those of the genus Azospirillum. In all the analyses, including phenotypic characterization using Biolog analysis and comparison of cellular fatty acids, this isolate was found to be different from the closely related species Azospirillum lipoferum, Azospirillum oryzae and Azospirillum brasilense. On the basis of these results, a novel species is proposed for this nitrogen-fixing strain. The name Azospirillum canadense sp. nov. is suggested with the type strain DS2T (=NCCB 100108T=LMG 23617T).

scholarly journals Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius

2006 ◽  
Vol 56 (5) ◽  
pp. 953-958 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Marc Vancanneyt ◽  
Anatoly M. Lysenko ◽  
Dong Sung Shin ◽  
...  
Keyword(s):  
Sea Urchin ◽  
New Genus ◽  
Gene Sequence ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Strongylocentrotus Intermedius ◽  
Respiratory Quinone ◽  
Nearest Neighbours ◽  
Gene Sequence Analysis ◽  
Major Respiratory Quinone

The taxonomic position of three novel marine, heterotrophic, pigmented and agarolytic bacteria with gliding motility, isolated from the sea urchin Strongylocentrotus intermedius, was investigated. 16S rRNA gene sequence analysis revealed that strains KMM 6166, KMM 6172T and KMM 6173 are members of the phylum Bacteroidetes; their nearest neighbours were Belliella baltica and Hongiella marincola (similarities of 94.5 and 93.6 %, respectively). The DNA G+C content of the strains was 44–45 mol%. The predominant fatty acids were C15 : 0 iso, C16 : 1 ω5c, C17 : 1 iso ω9c, C17 : 0 iso 3-OH and summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH). The major respiratory quinone was MK-7. Results of molecular experiments supported by phenotypic and chemotaxonomic data enabled the isolates to be classified as representatives of a novel species in a new genus, for which the name Echinicola pacifica gen. nov., sp. nov. is proposed. Echinicola pacifica is the type species of the genus Echinicola, and its type strain is KMM 6172T (=KCTC 12368T=LMG 23350T).

scholarly journals Streptococcus porci sp. nov., isolated from swine sources

2010 ◽  
Vol 60 (1) ◽  
pp. 104-108 ◽  
Author(s):  
A. I. Vela ◽  
M. Perez ◽  
L. Zamora ◽  
L. Palacios ◽  
L. Domínguez ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Sequence Divergence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Taxonomic Analysis ◽  
Gene Sequence Analysis

Two unidentified Gram-positive, catalase-negative, coccus-shaped organisms were recovered from pigs and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Streptococcus, although the organisms did not appear to correspond to any recognized species. Comparative 16S rRNA gene sequence studies confirmed this identification and showed that the nearest phylogenetic relatives of the unknown cocci were Streptococcus plurextorum 1956-02T and Streptococcus suis NCTC 10234T (97.9 and 96.0 % 16S rRNA gene sequence similarity, respectively). The new isolates were related most closely to S. suis CIP 103217T based on rpoB gene sequence analysis (<8 % sequence divergence). DNA–DNA pairing studies showed that one of the unidentified strains (2923-03T) displayed DNA relatedness values of 26.6 and 27.2 % with S. plurextorum CECT 7308T and S. suis NCTC 10234T, respectively. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from pigs be classified in the genus Streptococcus as members of Streptococcus porci sp. nov., with the type strain 2923-03T (=CECT 7374T =CCUG 55896T).

Ferriphaselus amnicola gen. nov., sp. nov., a neutrophilic, stalk-forming, iron-oxidizing bacterium isolated from an iron-rich groundwater seep

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 921-925 ◽  
Author(s):  
Shingo Kato ◽  
Sean Krepski ◽  
Clara Chan ◽  
Takashi Itoh ◽  
Moriya Ohkuma
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Content Type ◽  
Taxonomic Analysis ◽  
Iron Oxidizing Bacteria ◽  
Gene Sequence Analysis ◽  
Total Dna ◽  
Bacterium Strain

A neutrophilic, stalk-forming, iron-oxidizing bacterium, strain OYT1T, which was isolated from a groundwater seep in Ohyato Park, Tokyo, Japan, was subjected to taxonomic analysis. OYT1T was a motile, bean-shaped, Gram-negative bacterium that was able to grow at 8–30 °C (optimally at 25–30 °C) and at pH 5.6–7.3 (optimally at pH 6.1–6.5). The strain grew microaerobically and autotrophically. Major cellular fatty acids detected were C16 : 1ω7c/C16 : 1ω6c and C16 : 0. The total DNA G+C content was 57.6 mol%. 16S rRNA gene sequence analysis revealed that strain OYT1T was affiliated with the class Betaproteobacteria and clustered with iron-oxidizing bacteria isolated from groundwater seeps and wetlands and with uncultured clones detected in freshwater iron-rich environments. Based on the phenotypic and phylogenetic characteristics of strain OYT1T, we propose that the strain represents a novel species in a new genus, for which the name Ferriphaselus amnicola gen. nov., sp. nov. is proposed; the type strain of Ferriphaselus amnicola is OYT1T ( = JCM 18545T = DSM 26810T).

Amnibacterium kyonggiense gen. nov., sp. nov., a new member of the family Microbacteriaceae

2011 ◽  
Vol 61 (1) ◽  
pp. 155-159 ◽  
Author(s):  
Sun-Jung Kim ◽  
Sang-Seob Lee
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Diamino Acid ◽  
Taxonomic Approach

A Gram-positive, non-motile bacterium, designated KSL51201-037T, was isolated from Anyang stream, Republic of Korea, and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain KSL51201-037T belonged to the family Microbacteriaceae of the class Actinobacteria and exhibited 96.9 % gene sequence similarity to Labedella gwakjiensis KSW2-17T, 96.0 % to Leifsonia ginsengi wged11T and 95.9 % to Microterricola viridarii KV-677T. The G+C content of the genomic DNA was 72.7 mol%. Strain KSL51201-037T had l-2,4-diaminobutyric acid as the diagnostic cell-wall diamino acid, MK-11 and MK-12 as the major menaquinones, anteiso-C15 : 0 (47.8 %) and iso-C16 : 0 (24.0 %) as the major fatty acids and phosphatidylglycerol and three unknown phospholipids as the major polar lipids. On the basis of phenotypic and genotypic properties and phylogenetic distinctiveness, it is suggested that strain KSL51201-037T represents a novel species of a new genus in the family Microbacteriaceae for which the name Amnibacterium kyonggiense gen. nov., sp. nov. is proposed. The type strain of the type species is KSL51201-037T (=KEMC 51201-037T=JCM 16463T).

scholarly journals Fervidicoccus fontis Strain 3639Fd, the First Crenarchaeon Capable of Growth on Lipids

Microbiology ◽  
2021 ◽  
Vol 90 (4) ◽  
pp. 435-442
Author(s):  
A. I. Karaseva ◽  
A. G. Elcheninov ◽  
A. A. Perevalova ◽  
K. S. Zayulina ◽  
T. V. Kochetkova ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Gene Sequence ◽  
Thermal Spring ◽  
Rrna Gene ◽  
Oil Well ◽  
Strictly Anaerobic ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
Hydrolysis Of ◽  
The 16S Rrna Gene

Abstract— Up to now, ability of prokaryotes to grow on lipids has been shown only for bacteria. Thermococcus sibiricus, member of the phylum Euryarchaeota isolated from a high-temperature oil well and capable of growth on olive oil, is the only exception. The present work reports isolation of a pure culture of a strictly anaerobic archaeon, strain 3639Fd (=VKM B-3509, =KCTC 25228) from a Kamchatka thermal spring, capable of growth on various lipids (tributyrin, triolein, and sesame, cottonseed, and sunflower oil) at 70°C and pH 5.5–6.0. Growth on tributyrin resulted in formation of butyrate, CO2, and hydrogen. According to the results of the 16S rRNA gene sequence analysis and in silico DNA–DNA hybridization, the isolate was classified as a strain of Fervidicoccus fontis, an archaeon of the phylum Crenarchaeota. The closest characterized homologs of the α/β-hydrolases, encoded in the genomes of F. fontis 3639Fd and of the type strain of this species, Kam940T, were various carboxyl esterases (EC 3.1.1), the enzymes responsible for lipid hydrolysis. Thus, F. fontis is the first crenarchaeon able to obtain energy by hydrolysis of lipid substrates.

scholarly journals Bacillus chungangensis sp. nov., a halophilic species isolated from sea sand

2010 ◽  
Vol 60 (6) ◽  
pp. 1349-1352 ◽  
Author(s):  
Sung-Lim Cho ◽  
Min Young Jung ◽  
Mi-Hak Park ◽  
Wonyong Kim
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Sequence Divergence ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Data ◽  
Phenotypic Differences ◽  
Sea Sand ◽  
Gene Sequence Analysis ◽  
Halophilic Species

The taxonomic position of a Gram-stain-positive, endospore-forming, halophilic strain, designated CAU 348T, isolated from sea sand was investigated using a polyphasic approach. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 348T had the characteristics of the genus Bacillus. Comparative 16S rRNA gene sequence analysis showed that the organism formed a hitherto unknown subline within the genus Bacillus. Sequence divergence values of more than 4.3 % from other described Bacillus species, together with phenotypic differences, showed that the unidentified bacterium represents a previously unrecognized member of this genus. The genotypic and phenotypic data indicated that strain CAU 348T represents a novel species of the genus Bacillus, for which the name Bacillus chungangensis sp. nov. is proposed. The type strain is CAU 348T (=KCTC 13566T =CCUG 57835T).

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