scholarly journals Microscopic, cultural and molecular evidence of disseminated invasive aspergillosis involving the lungs and the gastrointestinal tract

2006 ◽  
Vol 55 (7) ◽  
pp. 961-964 ◽  
Author(s):  
R. S. Jayshree ◽  
M. Shafiulla ◽  
J. George ◽  
J. K. David ◽  
P. P. Bapsy ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Invasive Aspergillosis ◽  
Aspergillus Flavus ◽  
Molecular Evidence ◽  
Dna Analyses ◽  
Random Amplification ◽  
Fungal Hyphae ◽  
Gastrointestinal Aspergillosis ◽  
Disseminated Disease ◽  
First Time

A patient with acute promyelocytic leukaemia developed invasive aspergillosis post chemotherapy during a pancytopenic episode, clinically involving the lungs and the gastrointestinal tract. Dichotomously branched septate fungal hyphae were demonstrated microscopically in stools and sputa. Cultures of the samples yielded Aspergillus flavus, which were identical by RFLP and random amplification of polymorphic DNA analyses and antifungal MICs, proving disseminated disease. To the best of the author's knowledge, this is the first time that boluses of fungal hyphae have been demonstrated microscopically in the stools of a patient with gastrointestinal aspergillosis.

scholarly journals The T788G Mutation in thecyp51CGene Confers Voriconazole Resistance in Aspergillus flavus Causing Aspergillosis

2012 ◽  
Vol 56 (5) ◽  
pp. 2598-2603 ◽  
Author(s):  
Wei Liu ◽  
Yi Sun ◽  
Wei Chen ◽  
Weixia Liu ◽  
Zhe Wan ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Resistant Strain ◽  
Antifungal Susceptibility ◽  
Gene Replacement ◽  
Content Type ◽  
Replacement Method ◽  
First Choice ◽  
The One ◽  
A Site ◽  
First Time

ABSTRACTWith voriconazole (VRC) being approved as the first choice in treating invasive aspergillosis (IA) and its increasing use in treatment, a VRC-resistant strain ofAspergillus flavus, the second leading cause of IA afterAspergillus fumigatus, has emerged. The VRC-resistant strain ofA. flavuswas isolated for the first time from the surgical lung specimen of an IA patient with no response to VRC therapy. In order to ascertain the mechanism of VRC resistance, the azole target enzyme genes in this strain ofA. flavuswere cloned and sequenced, and 4 mutations generating amino acid residue substitutions were found in thecyp51Cgene. To further determine the role of this mutated gene for VRC resistance inA. flavus, anAgrobacterium tumefaciens-mediated gene replacement approach was applied. Consequently, the mutatedcyp51Cgene from thisA. flavusstrain was proven to confer the VRC resistance. Finally, to discern the one out of the four mutations in thecyp51Cgene that is responsible for contributing to VRC resistance, a site-directed gene mutagenesis procedure combined with a gene replacement method was performed. As a result, the T788G missense mutation in thecyp51Cgene was identified as responsible for VRC resistance inA. flavus. These findings indicated that the detection of this mutation inA. flavuscould serve as an indicator for physicians to avoid the use of VRC during IA treatment. Further comprehensive surveillance for antifungal susceptibility, as well as intensive study on the mechanism of azole resistance inA. flavuscausing IA, would be required to fully understand this mechanism.

scholarly journals Comparison and Utilization of Repetitive-Element PCR Techniques for Typing Lactobacillus Isolates from the Chicken Gastrointestinal Tract

2009 ◽  
Vol 75 (21) ◽  
pp. 6764-6776 ◽  
Author(s):  
David P. Stephenson ◽  
Robert J. Moore ◽  
Gwen E. Allison
Keyword(s):  
Gastrointestinal Tract ◽  
Lactobacillus Reuteri ◽  
Repetitive Element ◽  
Necrotic Enteritis ◽  
High Throughput Analysis ◽  
Strain Diversity ◽  
Disease Induction ◽  
First Time ◽  
Pcr Techniques ◽  
Type Strains

ABSTRACT Three repetitive-element PCR techniques were evaluated for the ability to type strains of Lactobacillus species commonly identified in the chicken gastrointestinal tract. Enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) produced species- and strain-specific profiles for Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus johnsonii, and Lactobacillus reuteri isolates. The technique typed strains within these species equally as well as pulsed-field gel electrophoresis. DNA concentration and quality did not affect the ERIC-PCR profiles, indicating that this method, unlike other high-resolution methods, can be adapted to high-throughput analysis of isolates. Subsequently, ERIC-PCR was used to type Lactobacillus species diversity of a large collection of isolates derived from chickens grown under commercial and necrotic enteritis disease induction conditions. This study has illustrated, for the first time, that there is great strain diversity within each Lactobacillus species present and has revealed that chickens raised under commercial conditions harbor greater species and strain diversity than chickens raised under necrotic enteritis disease induction conditions.

scholarly journals Four new corticioid species in Trechisporales (Basidiomycota) from East Asia and notes on phylogeny of the order

MycoKeys ◽  
2019 ◽  
Vol 48 ◽  
pp. 97-113
Author(s):  
Shi-Liang Liu ◽  
Hai-Xia Ma ◽  
Shuang-Hui He ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Phylogenetic Tree ◽  
East Asia ◽  
Molecular Evidence ◽  
Family Level ◽  
First Time

Four new species in Trechisporales from East Asia, Dextrinocystiscalamicola, Subulicystidiumacerosum, S.tropicum and Tubuliciumbambusicola, are described and illustrated, based on morphological and molecular evidence. The phylogeny of Trechisporales was inferred from a combined dataset of ITS-nrLSU sequences. In the phylogenetic tree, Sistotremastrum formed a family-level clade of its own, sister to the Hydnodontaceae clade formed by all other genera. Dextrinocystis, is for the first time, confirmed as a member of Hydnodontaceae. A key to all the accepted genera in Trechisporales is given.

Checklist of the Pipunculidae (Diptera) of mainland France: further faunistic records and description of a new species

Zootaxa ◽  
2021 ◽  
Vol 5067 (4) ◽  
pp. 517-547
Author(s):  
PHIL WITHERS ◽  
JOCELYN CLAUDE
Keyword(s):  
New Species ◽  
The Other ◽  
Dna Analyses ◽  
Precise Data ◽  
New Material ◽  
First Time ◽  
Private Collections ◽  
A New Species

Since the first faunistic study of the Pipunculidae (Diptera) of mainland France in 2006, new material from 37 natural sites and four private collections has been evaluated by the authors of this article. A total of 5739 specimens of Pipunculidae collected in France were examined, of which 5214 were identified to species by the authors, for a total of 114 species. DNA analyses were also carried out on some specimens to confirm their morphological identifications. The list of Pipunculidae recorded in mainland France is updated to include 140 species. Tomosvaryella estebani sp. nov. is described from material collected in France and Italy. Twenty-four species are also reported for the first time from France. The records of Cephalops (Semicephalops) carinatus (Verrall, 1901), Cephalosphaera (Cephalosphaera) furcata (Egger, 1860), Eudorylas terminalis (Thomson, 1870) and Jassidophaga villosa (Roser, 1840), without precise data in the literature, are now precisely located in France with this work. For the other species, we provide new post-2006 records.  

First record of the leafhopper genus Xenovarta Viraktamath (Hemiptera, Cicadellidae, Deltocephalinae) from India with description of a new species

Zootaxa ◽  
2018 ◽  
Vol 4532 (3) ◽  
pp. 444
Author(s):  
NARESH M. MESHRAM ◽  
STUTI STUTI ◽  
TAHSEEN RAZA HASHMI
Keyword(s):  
New Species ◽  
Agricultural Research ◽  
Indian Agricultural Research Institute ◽  
First Record ◽  
Molecular Evidence ◽  
New Delhi ◽  
Key To Species ◽  
Agricultural Research Institute ◽  
First Time ◽  
A New Species

The genus Xenovarta Viraktamath is recorded from India for the first time and Xenovarta viraktamathi sp. nov. from India is described and illustrated. Materials are deposited in the National Pusa Collection, Division of Entomology, ICAR-Indian Agricultural Research Institute (IARI), New Delhi, India. A key to species of the genus are provided, with molecular evidence of the specimen in the form of partial mtCOI sequences. 

A morphological, enzymatic and metabolic approach to elucidate apoptotic-like cell death in fungi exposed to h- and α-molybdenum trioxide nanoparticles

Nanoscale ◽  
2018 ◽  
Vol 10 (44) ◽  
pp. 20702-20716 ◽  
Author(s):  
Clemencia Chaves-Lopez ◽  
Hang N. Nguyen ◽  
Rodrigo C. Oliveira ◽  
Enrico T. Nadres ◽  
Antonello Paparella ◽  
...  
Keyword(s):  
Cell Death ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Molybdenum Trioxide ◽  
Fungal Strains ◽  
First Time

The present study compares for the first time the effects of h-MoO3and α-MoO3against two fungal strains:Aspergillus nigerandAspergillus flavus.

scholarly journals Asperienes A–D, Bioactive Sesquiterpenes from the Marine-Derived Fungus Aspergillus flavus

Marine Drugs ◽  
2019 ◽  
Vol 17 (10) ◽  
pp. 550 ◽  
Author(s):  
Yun-Feng Liu ◽  
Yu-Fei Yue ◽  
Li-Xi Feng ◽  
Hua-Jie Zhu ◽  
Fei Cao
Keyword(s):  
Cell Lines ◽  
Aspergillus Flavus ◽  
Solid Medium ◽  
Biological Activities ◽  
Antitumor Agent ◽  
Minor Components ◽  
Electronic Circular Dichroism ◽  
The Future ◽  
Ic50 Values ◽  
First Time

Marine-derived fungi of the genera Aspergillus could produce novel compounds with significant bioactivities. Among these fungi, the strain Aspergillus flavus is notorious for its mutagenic mycotoxins production. However, some minor components with certain toxicities from A. flavus have not been specifically surveyed and might have potent biological activities. Our investigation of the marine-derived fungus Aspergillus flavus CF13-11 cultured in solid medium led to the isolation of four C-6′/C-7′ epimeric drimane sesquiterpene esters, asperienes A–D (1–4). Their absolute configurations were assigned by electronic circular dichroism (ECD) and Snatzke’s methods. This is the first time that two pairs of C-6′/C-7′ epimeric drimane sesquiterpene esters have successfully been separated. Aperienes A–D (1–4) displayed potent bioactivities towards four cell lines with the IC50 values ranging from 1.4 to 8.3 μM. Interestingly, compounds 1 and 4 exhibited lower toxicities than 2 and 3 toward normal GES-1 cells, indicating more potential for development as an antitumor agent in the future.

scholarly journals A note on the systematic position of the Bathynellacea (Crustacea, Malacostraca) using molecular evidence

2002 ◽  
Vol 71 (4) ◽  
pp. 123-129 ◽  
Author(s):  
Ana Isabel Camacho ◽  
Isabel Rey ◽  
Beatriz A. Dorda ◽  
Annie Machordom ◽  
Antonio G. Valdecasas
Keyword(s):  
Molecular Data ◽  
Systematic Position ◽  
Molecular Evidence ◽  
Gene Fragment ◽  
16S Rdna Gene ◽  
Internal Morphology ◽  
Mt 16S Rdna ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Molecular data for the mt 16S rDNA gene fragment of a bathynellacean is here presented for the first time and used to analyze the relationship of the group within the crustacean class Malacostraca (Arthropoda, Bathynellacea). Two contrasting views have classified the bathynelids as being either within the order Syncarida or in a separate super-order Podophallocarida belonging to the infra-class Eonomostraca, a disagreement based mainly on debates over external and internal morphology. The preliminary analyses offered here in question the placement of this Bathynellacea within the Syncarida, and suggest the need for a further study of relationships among the malacostracan groups.

On a collection of hydroids (Cnidaria, Hydrozoa) from the southwest coast of Florida, USA

Zootaxa ◽  
2019 ◽  
Vol 4689 (1) ◽  
pp. 1-141
Author(s):  
DALE R. CALDER
Keyword(s):  
Chesapeake Bay ◽  
Zoological Nomenclature ◽  
Molecular Evidence ◽  
International Code ◽  
Caribbean Coast ◽  
Western Atlantic ◽  
Southwest Coast ◽  
The Caribbean ◽  
First Time ◽  
One New Species

Sixty species of hydroids, assigned to 24 families and 39 genera, are recognized and discussed in a collection of material from the southwest coast of Florida. One new species (Clytia joycei) is described from turtlegrass (Thalassia testudinum) and reported as well from the Caribbean coast of Panama. Under provisions of the First Reviser Principle in the International Code of Zoological Nomenclature, Antennopsis nigra Nutting, 1900 is assigned precedence over its simultaneous synonym A. longicorna Nutting, 1900. Also included as a synonym of A. nigra is A. sinuosa Fraser, 1947b. The species is now assigned to Nemertesia Lamouroux, 1816, as N. nigra. Following Reversal of Precedence provisions in the code, the well-known name Halecium nanum Alder, 1859 is designated as valid and conserved as a nomen protectum, while Hydra articulata Bosc, 1797 is relegated to a nomen oblitum. The genus Monotheca Nutting, 1900 is upheld as valid on the basis of both morphological and molecular evidence. Sertularia pourtalesi Nutting, 1904, a seldom-reported species, is assigned to Dynamena Lamouroux, 1812 and recognized as distinct from D. disticha (Bosc, 1802). Calyptospadix cerulea Clarke, 1882 and Bimeria franciscana Torrey, 1902 (also known as Garveia franciscana) are taken to be conspecific, with Calyptospadix Clarke, 1882 treated as a valid genus. Confusion over the identity of Lovenella gracilis Clarke, 1882 in literature on North American hydroids is addressed, and topotypic specimens from the Chesapeake Bay region are illustrated to document characters that distinguish the species. Campanularia colombiana (Wedler, 1976) is reported for the first time outside the Caribbean coast of Colombia. The fauna studied here consists largely of species known to occur in the tropical and subtropical western Atlantic. [Zoobank URL: urn:lsid:zoobank.org:act:4C926BE2-D75D-449A-9EAD-14CADACFFADD] 

Sign in / Sign up

Export Citation Format

Share Document