scholarly journals Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus

2005 ◽  
Vol 86 (7) ◽  
pp. 2107-2113 ◽  
Author(s):  
Marcel Prins ◽  
Dick Lohuis ◽  
Arjen Schots ◽  
Rob Goldbach
Keyword(s):  
Phage Display ◽  
Plant Virus ◽  
Rational Design ◽  
Tomato Spotted Wilt Virus ◽  
Single Chain ◽  
Resistance Strategies ◽  
Single Chain Antibodies ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Durability Of Resistance

Rational design of antibodies targeting essential viral proteins can complement the palette of antiviral resistance strategies. Here, stable and high expression of single-chain monoclonal antibodies targeting the nucleoprotein of the economically important plant virus Tomato spotted wilt virus, a protein that is involved in multiple steps in the viral infection cycle, is reported. High cytoplasmic expression levels of three selected phage display-derived anti-viral single-chain antibodies were established. Of these antibodies, two led to high levels of resistance against this plant virus. Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field.

scholarly journals Application of Phage Display in Selecting Tomato spotted wilt virus-Specific Single-Chain Antibodies (scFvs) for Sensitive Diagnosis in ELISA

2000 ◽  
Vol 90 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Remko A. Griep ◽  
Marcel Prins ◽  
Charlotte van Twisk ◽  
Hans J. H. G. Keller ◽  
Randolf J. Kerschbaumer ◽  
...  
Keyword(s):  
Phage Display ◽  
Dna Sequences ◽  
Tomato Spotted Wilt Virus ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Single Chain ◽  
Spot Virus ◽  
Single Chain Antibodies ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

A panel of recombinant single-chain antibodies (scFvs) against structural proteins of Tomato spotted wilt virus (TSWV) was retrieved from a human combinatorial scFv antibody library using the novel phage display technique. After subcloning the encoding DNA sequences in the expression vector pSKAP/S, which allowed the scFvs to be expressed as alkaline phosphatase fusion proteins, 17 different scFv antibodies were obtained. Of these, 12 scFvs were directed against the nucleoprotein (N) and 5, putatively, against the glycoproteins (G1 and G2). Five of the N-specific antibodies cross-reacted with two other tospoviruses (Tomato chlorotic spot virus and Groundnut ringspot virus), but none recognized the more distantly related tospoviruses Impatiens necrotic spot virus, Watermelon silverleaf mottle virus, Iris yellow spot virus, or Physalis severe mottle virus. The successful use of one of the antibodies as coating and detection reagent in a double-antibody sandwich enzyme-linked immunosorbent assay showed the potential of the phage display system in obtaining antibodies for routine TSWV diagnosis.

The silencing suppressor (NSs) protein of the plant virus Tomato spotted wilt virus enhances heterologous protein expression and baculovirus pathogenicity in cells and lepidopteran insects

2015 ◽  
Vol 160 (11) ◽  
pp. 2873-2879 ◽  
Author(s):  
Virgínia Carla de Oliveira ◽  
Fabricio da Silva Morgado ◽  
Daniel Mendes Pereira Ardisson-Araújo ◽  
Renato Oliveira Resende ◽  
Bergmann Morais Ribeiro
Keyword(s):  
Protein Expression ◽  
Plant Virus ◽  
Tomato Spotted Wilt Virus ◽  
Heterologous Protein ◽  
Heterologous Protein Expression ◽  
Silencing Suppressor ◽  
Tomato Spotted Wilt ◽  
Lepidopteran Insects ◽  
Wilt Virus ◽  
In Cells

scholarly journals Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 320
Author(s):  
Alexander Nilon ◽  
Karl Robinson ◽  
Hanu R. Pappu ◽  
Neena Mitter
Keyword(s):  
Rna Interference ◽  
Tomato Spotted Wilt Virus ◽  
Cultural Practices ◽  
Management Strategies ◽  
Current Status ◽  
Horticultural Crops ◽  
Yield And Quality ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.

Lipid in a Purified Preparation of Tomato Spotted Wilt Virus

Nature ◽  
1964 ◽  
Vol 203 (4945) ◽  
pp. 671-672 ◽  
Author(s):  
RUPERT J. BEST ◽  
GERARD F. KATEKAR
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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