Replication of Dengue Virus Type 2 in Aedes albopictus Cell Culture

A full-length infectious cDNA clone (ic) was constructed from the genome of the dengue virus type 2 (DENV-2) Jamaica83 1409 strain, pBAC1409ic, by using a bacterial artifical chromosome plasmid system. Infectious virus was generated and characterized for growth in cell culture and for infection in Aedes aegypti mosquitoes. During construction, an isoleucine to methionine (Ile→Met) change was found at position 6 in the envelope glycoprotein sequence between low- and high-passage DENV-2 1409 strains. In vitro-transcribed genomic RNA of 1409ic with E6-Ile produced infectious virions following electroporation in mosquito cells, but not mammalian cells, while 1409ic RNA with an E6-Met mutation produced virus in both cell types. Moreover, DENV-2 1409 with the E6-Ile residue produced syncytia in C6/36 cell culture, whereas viruses with E6-Met did not. However, in vitro cell culture-derived growth-curve data and in vivo mosquito-infection rates revealed that none of the analysed DENV-2 strains differed from each other.

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Quantitative Analysis of Replication and Tropisms of Dengue Virus Type 2 in Aedes albopictus

American Journal of Tropical Medicine and Hygiene ◽

10.4269/ajtmh.2010.10-0193 ◽

2010 ◽

Vol 83 (3) ◽

pp. 700-707 ◽

Cited By ~ 23

Author(s):

Meichun Zhang ◽

Ximei Zhan ◽

Ai He ◽

Ming Gan ◽

Jing Liu ◽

...

Keyword(s):

Quantitative Analysis ◽

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Dengue Virus Type 2

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RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol.

Jurnal Kimia Riset ◽

10.20473/jkr.v3i1.7455 ◽

2018 ◽

Vol 3 (1) ◽

pp. 52

Author(s):

Yovilianda Maulitiva Untoro ◽

Teguh Hari Sucipto ◽

Harsasi Setyawati ◽

Siti Churrotin ◽

Ilham Harlan Amarullah ◽

...

Keyword(s):

Aqueous Solution ◽

Aedes Aegypti ◽

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Rna Isolation ◽

Guanidinium Thiocyanate ◽

The World ◽

Dengue Virus Type 2

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

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Expression of Concern: Antivirus effectiveness of ivermectin on dengue virus type 2 in Aedes albopictus

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0010117 ◽

2022 ◽

Vol 16 (1) ◽

pp. e0010117

Author(s):

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Dengue Virus Type 2

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Dengue infection in Paracambi, State of Rio de Janeiro, 1990-1995

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86821997000500005 ◽

1997 ◽

Vol 30 (5) ◽

pp. 379-383 ◽

Cited By ~ 8

Author(s):

Rivaldo Venâncio da Cunha ◽

Renato C. Maspero ◽

Marize P. Miagostovich ◽

Eliane S.M. de Araújo ◽

Daniele da C. Luz ◽

...

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Rio De Janeiro ◽

Dengue Infection ◽

Haemagglutination Inhibition ◽

Older Children ◽

Dengue Virus Type 2

A seroepidemiological survey was carried out during 1994 in the municipality of Paracambi, state of Rio de Janeiro. Haemagglutination inhibition test positivity was detected in 145 out of 370 (39.2%) schoolchildren. The frequency of positive test by sex was 53.8% (78/145) female and 46.2% (67/145) male. Distribution by age showed the increasing of antibody posivity in older children. Strains of dengue virus type 1 and dengue virus type 2 were isolated before (1990) showing the co-circulation of both serotypes in that area. The house index infestation of Aedes albopictus and Aedes aegypti has been determined.

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INFECTION AND DISSEMINATION OF DENGUE VIRUS TYPE 2 IN AEDES AEGYPTI, AEDES ALBOPICTUS, AND AEDES SCUTELLARIS FROM THE TORRES STRAIT, AUSTRALIA

Journal of the American Mosquito Control Association ◽

10.2987/5598.1 ◽

2007 ◽

Vol 23 (4) ◽

pp. 383-388 ◽

Cited By ~ 25

Author(s):

PETER R. MOORE ◽

PETRINA H. JOHNSON ◽

GREG A. SMITH ◽

SCOTT A. RITCHIE ◽

ANDREW F. VAN DEN HURK

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Torres Strait ◽

Dengue Virus Type 2

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Antivirus effectiveness of ivermectin on dengue virus type 2 in Aedes albopictus

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0006934 ◽

2018 ◽

Vol 12 (11) ◽

pp. e0006934 ◽

Cited By ~ 11

Author(s):

Tie-Long Xu ◽

Yin Han ◽

Wei Liu ◽

Xing-Ya Pang ◽

Bin Zheng ◽

...

Keyword(s):

Dengue Virus ◽

Virus Type ◽

Aedes Albopictus ◽

Dengue Virus Type 2

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ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL

Indonesian Journal of Tropical and Infectious Disease ◽

10.20473/ijtid.v6i4.3806 ◽

2017 ◽

Vol 6 (4) ◽

pp. 84 ◽

Cited By ~ 5

Author(s):

Teguh Hari Sucipto ◽

Siti Churrotin ◽

Harsasi Setyawati ◽

Tomohiro Kotaki ◽

Fahimah Martak ◽

...

Keyword(s):

Cell Culture ◽

Dengue Virus ◽

Vero Cell ◽

Virus Type ◽

Inhibitory Concentration ◽

Enzyme Linked Immunosorbent Assay ◽

Antiviral Compound ◽

Chloride Dihydrate ◽

Dengue Virus Type 2

Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 μg/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.

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