scholarly journals Computer-Assisted Reconstruction and Motion Analysis of the Three-Dimensional Cell

2003 ◽  
Vol 3 ◽  
pp. 827-841 ◽  
Author(s):  
David R. Soll ◽  
Deborah Wessels ◽  
Paul J. Heid ◽  
Edward Voss
Keyword(s):  
3D Reconstruction ◽  
Motion Analysis ◽  
Three Dimensional ◽  
Computer Assisted ◽  
3D Analysis ◽  
Time Intervals ◽  
Abnormal Cells ◽  
3D Information ◽  
Short Time ◽  
Microscopic Techniques

Even though several microscopic techniques provide three-dimensional (3D) information on fixed and living cells, the perception persists that cells are two-dimensional (2D). Cells are, in fact, 3D and their behavior, including the extension of pseudopods, includes an important 3D component. Although treating the cell as a 2D entity has proven effective in understanding how cells locomote, and in identifying defects in a variety of mutant and abnormal cells, there are cases in which 3D reconstruction and analysis are essential. Here, we describe advanced computer-assisted 3D reconstruction and motion analysis programs for both individual live, crawling cells and developing embryos. These systems (3D-DIAS, 3D-DIASemb) can be used to reconstruct and motion analyze at short time intervals the nucleus and pseudopodia as well as the entire surface of a single migrating cell, or every cell and nucleus in a developing embryo. Because all images are converted to mathematical representations, a variety of motility and dynamic morphology parameters can be computed that have proven quite valuable in the identification of mutant behaviors. We also describe examples of mutant behaviors in Dictyostelium that were revealed through 3D analysis.

A Novel Method for Automated Acquisition of Tilt Series for Electron Tomography Based on Pre-Calibration of the Specimen Stage

2000 ◽  
Vol 6 (S2) ◽  
pp. 1148-1149
Author(s):  
U. Ziese ◽  
A.H. Janssen ◽  
T.P. van der Krift ◽  
A.G. van Balen ◽  
W.J. de Ruijter ◽  
...  
Keyword(s):  
High Resolution ◽  
3D Reconstruction ◽  
Cell Biology ◽  
Electron Tomography ◽  
Three Dimensional ◽  
Imaging Method ◽  
3D Images ◽  
Tilt Series ◽  
3D Information ◽  
Structural Arrangements

Electron tomography is a three-dimensional (3D) imaging method with transmission electron microscopy (TEM) that provides high-resolution 3D images of structural arrangements. Conventional TEM images are in first approximation mere 2D-projections of a 3D sample under investigation. With electron tomographya series of images is acquired of a sample that is tilted over a large angular range (±70°) with small angular tilt increments (so called tilt-series). For the subsequent 3D-reconstruction, the images of the tilt series are aligned relative to each other and the 3D-reconstruction is computed. Electron tomography is the only technique that can provide true 3D information with nm-scale resolution of individual and unique samples. For (cell) biology and material science applications the availability of high-resolution 3D images of structural arrangements within individual samples provides unique architectural information that cannot be obtained otherwise. Routine application of electron tomography will comprise a major revolutionary step forward in the characterization of complex materials and cellular arrangements.

False ankylosis of the temporomandibular joint in a cat

2015 ◽  
Vol 28 (06) ◽  
pp. 455-458 ◽  
Author(s):  
N. Jamet ◽  
L. Larguier
Keyword(s):  
3D Reconstruction ◽  
Temporomandibular Joint ◽  
Objective Assessment ◽  
Three Dimensional ◽  
Computer Assisted ◽  
Office Visits ◽  
Computer Assisted Tomography ◽  
Postoperative Physical Therapy ◽  
One Year ◽  
Joint Range

SummaryObjective: To describe the use of two- dimensional computer-assisted tomography (CT) with three-dimensional (3D) reconstruction in the diagnosis and planning of surgical treatment of a case of false ankylosis of the temporomandibular joint.Case report: A young European Shorthaired cat was presented with the complaint of inability to eat and open its jaws. A CT scan with 3D reconstruction allowed visualization of the lesion which was causing extra- articular ankylosis of the temporomandibular joint. Surgery was performed to resect an osseous lesion of the zygomatic arch, thus freeing the temporomandibular joint. Postoperative physical therapy was initiated immediately following surgery, and then carried out by the owner with a one year follow-up. Clinical examination of the cat was performed during regular office visits (at 1 month and 3 months following surgery), which allowed objective assessment of postoperative recuperation. At the end of a year, the owners reported that the cat had maintained sufficient jaw opening without any signs consistent with chronic pain.Conclusion: Computed tomography scan with 3D reconstruction allowed planning of the surgical correction of extra-articular ankylosis of the temporomandibular joint, and in this case condylectomy was avoided, since temporomandibular joint range-of-motion was maintained.

Three-dimensional reconstruction based on images from spiral high-resolution computed tomography of the temporal bone: anatomy and clinical application

2005 ◽  
Vol 119 (9) ◽  
pp. 693-698 ◽  
Author(s):  
Beom-Cho Jun ◽  
Sun-Wha Song ◽  
Ju-Eun Cho ◽  
Chan-Soon Park ◽  
Dong-Hee Lee ◽  
...  
Keyword(s):  
Computed Tomography ◽  
High Resolution ◽  
3D Reconstruction ◽  
Temporal Bone ◽  
Three Dimensional ◽  
Image Data ◽  
Computer Assisted ◽  
Surface Rendering ◽  
High Resolution Computed Tomography

The aim of this study was to investigate the usefulness of a three-dimensional (3D) reconstruction of computed tomography (CT) images in determining the anatomy and topographic relationship between various important structures. Using 40 ears from 20 patients with various otological diseases, a 3D reconstruction based on the image data from spiral high-resolution CT was performed by segmentation, volume-rendering and surface-rendering algorithms on a personal computer. The 3D display of the middle and inner ear structures was demonstrated in detail. Computer-assisted measurements, many of which could not be easily measured in vivo, of the reconstructed structures provided accurate anatomic details that improved the surgeon’s understanding of spatial relationships. A 3D reconstruction of temporal bone CT might be useful for education and increasing understanding of the anatomical structures of the temporal bone. However, it will be necessary to confirm the correlation between the 3D reconstructed images and histological sections through a validation study.

A 3D Reconstruction Method Based on Binocular View Geometry

2010 ◽  
Vol 33 ◽  
pp. 299-303
Author(s):  
Zhong Yan Liu ◽  
Guo Quan Wang ◽  
Dong Ping Wang
Keyword(s):  
3D Reconstruction ◽  
Three Dimensional ◽  
Reconstruction Method ◽  
Feature Points ◽  
Imaging Model ◽  
Extrinsic Parameters ◽  
Image Acquisition System ◽  
Rearview Mirror ◽  
3D Information ◽  
Intrinsic And Extrinsic Parameters

A method was proposed to gain three-dimensional (3D) reconstruction based on binocular view geometry. Images used to calibrate cameras and reconstruct car’s rearview mirror by image acquisition system, by calibration image, a camera's intrinsic and extrinsic parameters, projective and fundamental matrixes were drawn by Matlab7.1;the collected rearview mirror images is pretreated to draw refined laser, extracted feature points, find the very appropriate match points by epipolar geometry principle; according to the camera imaging model to calculate the coordinates of space points, display point cloud, fitting space points to reconstruct car’s rearview mirror; experimental results show this method can better restore the car’s rearview mirror of 3D information.

scholarly journals 3D Reconstruction of Exposed Underground Utilities Using Photogrammetric Methods

2021 ◽  
Author(s):  
Wensong Hu
Keyword(s):  
3D Reconstruction ◽  
Digital Images ◽  
Low Cost ◽  
Three Dimensional ◽  
Lookup Table ◽  
Image Point ◽  
Digital Cameras ◽  
Study Results ◽  
Underground Utilities ◽  
3D Information

This thesis addresses the topic of three-dimensional (3D) reconstruction of exposed underground utilities using photogrammetric methods. Research on this topic is mainly motivated by the need for improved information on the location of underground utilities and, thus, to provide reliable information for the management of buried assets. In this thesis, a system of photogrammetric software programs is developed for 3D reconstruction of underground utilities. Camera calibration programs are used for computing interior elements and lens distortion coefficients of digital cameras and saving them in a lookup table (LUT). The accuracy of calibrated image coordinates satisfies the photogrammetric processing demand. An automatic image point detection method is proposed and achieved in these programs. External orientation programs are used for calculating exterior elements of the digital images. Based on geographic information system (GIS) and global positioning system (GPS) techniques, a new ground control points (GCPs) collection method is proposed and implemented in these programs. A 3D reconstruction program provides corresponding functions to obtain and edit 3D information of underground utilities. Epipolar lines are employed as an assisting tool that helps operators easily find homologous points from different digital images. The study results indicate that photogrammetric methods for reconstructing 3D information of underground utilities are effective and low cost.

scholarly journals Adaptive Fine Distortion Correction Method for Stereo Images of Skin Acquired with a Mobile Phone

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4492
Author(s):  
Cho-I Moon ◽  
Onseok Lee
Keyword(s):  
Mobile Phone ◽  
Stereo Matching ◽  
Three Dimensional ◽  
Skin Surface ◽  
Correction Method ◽  
Distortion Correction ◽  
Stereo Image ◽  
3D Analysis ◽  
Stereo Images ◽  
3D Information

With the development of the mobile phone, we can acquire high-resolution images of the skin to observe its detailed features using a mobile camera. We acquire stereo images using a mobile camera to enable a three-dimensional (3D) analysis of the skin surface. However, geometric changes in the observed skin structure caused by the lens distortion of the mobile phone result in a low accuracy of the 3D information extracted through stereo matching. Therefore, our study proposes a Distortion Correction Matrix (DCM) to correct the fine distortion of close-up mobile images, pixel by pixel. We verified the correction performance by analyzing the results of correspondence point matching in the stereo image corrected using the DCM. We also confirmed the correction results of the image taken at the five different working distances and derived a linear regression model for the relationship between the angle of the image and the distortion ratio. The proposed DCM considers the distortion degree, which appears to be different in the left and right regions of the image. Finally, we performed a fine distortion correction, which is difficult to check with the naked eye. The results of this study can enable the accurate and precise 3D analysis of the skin surface using corrected mobile images.

scholarly journals Evaluating Feature Extraction Methods with Synthetic Noise Patterns for Image-Based Modelling of Texture-Less Objects

2020 ◽  
Vol 12 (23) ◽  
pp. 3886
Author(s):  
Jahanzeb Hafeez ◽  
Jaehyun Lee ◽  
Soonchul Kwon ◽  
Sungjae Ha ◽  
Gitaek Hur ◽  
...  
Keyword(s):  
Feature Extraction ◽  
3D Reconstruction ◽  
Feature Matching ◽  
Low Cost ◽  
Three Dimensional ◽  
Extraction Methods ◽  
Planar Surfaces ◽  
Vision Sensors ◽  
3D Printed ◽  
3D Information

Image-based three-dimensional (3D) reconstruction is a process of extracting 3D information from an object or entire scene while using low-cost vision sensors. A structure-from-motion coupled with multi-view stereo (SFM-MVS) pipeline is a widely used technique that allows 3D reconstruction from a collection of unordered images. The SFM-MVS pipeline typically comprises different processing steps, including feature extraction and feature matching, which provide the basis for automatic 3D reconstruction. However, surfaces with poor visual texture (repetitive, monotone, etc.) challenge the feature extraction and matching stage and affect the quality of reconstruction. The projection of image patterns while using a video projector during the image acquisition process is a well-known technique that has been shown to be successful for such surfaces. In this study, we evaluate the performance of different feature extraction methods on texture-less surfaces with the application of synthetically generated noise patterns (images). Seven state-of-the-art feature extraction methods (HARRIS, Shi-Tomasi, MSER, SIFT, SURF, KAZE, and BRISK) are evaluated on problematic surfaces in two experimental phases. In the first phase, the 3D reconstruction of real and virtual planar surfaces evaluates image patterns while using all feature extraction methods, where the patterns with uniform histograms have the most suitable morphological features. The best performing pattern from Phase One is used in Phase Two experiments in order to recreate a polygonal model of a 3D printed object using all of the feature extraction methods. The KAZE algorithm achieved the lowest standard deviation and mean distance values of 0.0635 mm and −0.00921 mm, respectively.

scholarly journals 3D Reconstruction of Exposed Underground Utilities Using Photogrammetric Methods

2021 ◽  
Author(s):  
Wensong Hu
Keyword(s):  
3D Reconstruction ◽  
Digital Images ◽  
Low Cost ◽  
Three Dimensional ◽  
Lookup Table ◽  
Image Point ◽  
Digital Cameras ◽  
Study Results ◽  
Underground Utilities ◽  
3D Information

This thesis addresses the topic of three-dimensional (3D) reconstruction of exposed underground utilities using photogrammetric methods. Research on this topic is mainly motivated by the need for improved information on the location of underground utilities and, thus, to provide reliable information for the management of buried assets. In this thesis, a system of photogrammetric software programs is developed for 3D reconstruction of underground utilities. Camera calibration programs are used for computing interior elements and lens distortion coefficients of digital cameras and saving them in a lookup table (LUT). The accuracy of calibrated image coordinates satisfies the photogrammetric processing demand. An automatic image point detection method is proposed and achieved in these programs. External orientation programs are used for calculating exterior elements of the digital images. Based on geographic information system (GIS) and global positioning system (GPS) techniques, a new ground control points (GCPs) collection method is proposed and implemented in these programs. A 3D reconstruction program provides corresponding functions to obtain and edit 3D information of underground utilities. Epipolar lines are employed as an assisting tool that helps operators easily find homologous points from different digital images. The study results indicate that photogrammetric methods for reconstructing 3D information of underground utilities are effective and low cost.

scholarly journals Ultrahigh Voltage Electron Microscopy Links Neuroanatomy and Neuroscience/Neuroendocrinology

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Hirotaka Sakamoto ◽  
Mitsuhiro Kawata
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Ultrastructural Level ◽  
Biological Research ◽  
3D Analysis ◽  
Small Areas ◽  
Voltage Electron ◽  
Ultrathin Sections ◽  
Chemical Neuroanatomy ◽  
3D Information

The three-dimensional (3D) analysis of anatomical ultrastructures is extremely important in most fields of biological research. Although it is very difficult to perform 3D image analysis on exact serial sets of ultrathin sections, 3D reconstruction from serial ultrathin sections can generally be used to obtain 3D information. However, this technique can only be applied to small areas of a specimen because of technical and physical difficulties. We used ultrahigh voltage electron microscopy (UHVEM) to overcome these difficulties and to study the chemical neuroanatomy of 3D ultrastructures. This methodology, which links UHVEM and light microscopy, is a useful and powerful tool for studying molecular and/or chemical neuroanatomy at the ultrastructural level.

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