scholarly journals Rapid Detection of Norovirus Using Paper-based Microfluidic Device

2017 ◽  
Author(s):  
Xuan Weng ◽  
Suresh Neethirajan
Keyword(s):  
Microfluidic Device ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cost Effective ◽  
Quantitative Detection ◽  
Multi Walled Carbon Nanotubes ◽  
Low Sensitivity ◽  
Walled Carbon Nanotubes ◽  
Analytical Approaches

AbstractNoroviruses (NoV) are the leading cause of outbreak of acute gastroenteritis worldwide. A substantial effort has been made in the development of analytical devices for rapid and sensitive food safety monitoring via the detection of foodborne bacteria, viruses and parasites. Conventional analytical approaches for noroviruses suffer from some critical weaknesses: labor-intensive, time-consuming, and relatively low sensitivity. In this study, we developed a rapid and highly sensitive biosensor towards point-of-care device for noroviruses based on 6-carboxyfluorescein (6-FAM) labeled aptamer and nanomaterials, multi-walled carbon nanotubes (MWCNTs) and graphene oxide (GO). In an assay, the fluorescence of 6-FAM labeled aptamer was quenched by MWCNTs or GO via fluorescence resonance energy transfer (FRET). In the presence of norovirus, the fluorescence would be recovered due to the release of the 6-FAM labeled aptamer from MWCNTs or GO. An easy-to-make paper-based microfluidic platform made by nitrocellulose membrane was used to conduct the assay. The quantitative detection of norovirus virus-like particles (NoV VLPs) was successfully performed. A linear range of 0-12.9 μg/mL with a detection limit of 40 pM and 30 pM was achieved for the MWCNTs and GO based paper sensors, respectively. The results suggested the developed paper-based microfluidic device is simple, cost-effective and holds the potential of rapid in situ visual determination for noroviruses with remarkable sensitivity and specificity, which provides a new way for early identification of NoV and thereby an early intervention for preventing the spread of an outbreak.

scholarly journals Nanotechnology-Based Approaches for the Detection of SARS-CoV-2

2020 ◽  
Vol 2 ◽  
Author(s):  
Ritika Gupta ◽  
Poonam Sagar ◽  
Nitesh Priyadarshi ◽  
Sunaina Kaul ◽  
Rajat Sandhir ◽  
...  
Keyword(s):  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Cost Effective ◽  
Diagnostic Techniques ◽  
Localized Surface Plasmon ◽  
Diagnostic Tools ◽  
Diagnostic Systems ◽  
Economic Activities ◽  
Low Sensitivity

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic has been validated as an extreme clinical calamity and has affected several socio-economic activities globally. Proven transmission of this virus occurs through airborne droplets from an infected person. The recent upsurge in the number of infected individuals has already exceeded the number of intensive care beds available to patients. These extraordinary circumstances have elicited the need for the development of diagnostic tools for the detection of the virus and, hence, prevent the spread of the disease. Early diagnosis and effective immediate treatment can reduce and prevent an increase in the number of cases. Conventional methods of detection such as quantitative real-time polymerase chain reaction and chest computed tomography scans have been used extensively for diagnostic purposes. However, these present several challenges, including prolonged assay requirements, labor-intensive testing, low sensitivity, and unavailability of these resources in remote locations. Such challenges urgently require fast, sensitive, and accurate diagnostic techniques for the timely detection and treatment of coronavirus disease 2019 (COVID-19) infections. Point-of-care biosensors that include paper- and chip-based diagnostic systems are rapid, cost-effective, and user friendly. In this article nanotechnology-based potential biosensors for SARS-CoV-2 diagnosis are discussed with particular emphasis on a lateral flow assay, a surface-enhanced Raman scattering-based biosensor, a localized surface plasmon resonance-based biosensor, Förster resonance energy transfer, an electrochemical biosensor, and artificial intelligence-based biosensors. Several biomolecules, such as nucleic acids, antibodies/enzymes, or aptamers, can serve as potential detection molecules on an appropriate platform, such as graphene oxide, nanoparticles, or quantum dots. An effective biosensor can be developed by using appropriate combinations of nanomaterials and technologies.

scholarly journals Therapeutic Drug Monitoring: Performance of a FRET-Based Point-Of-Care Immunoassay for the Quantitation of Infliximab and Adalimumab in Blood

2020 ◽  
Author(s):  
Edgar Ong ◽  
Ruo Huang ◽  
Richard Kirkland ◽  
Stefan Westin ◽  
Jared Salbato ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Whole Blood ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Drug Level ◽  
Analytical Performance ◽  
Quantitative Detection ◽  
Therapeutic Drug

<p>Two fast (<5 min), time-resolved fluorescence resonance energy transfer (FRET)-based immunoassays (Procise IFX™ and Procise ADL™) were developed for the quantitative detection of infliximab (IFX), adalimumab (ADL), and their respective biosimilars for use in therapeutic drug monitoring (TDM) using 20 µL of finger prick whole blood at the point-of-care or whole blood/serum in a central lab. Studies were performed to characterize analytical performance of the Procise IFX and the Procise ADL assays on the ProciseDx™ analyzer.</p> <p><br></p><p>The Procise IFX and Procise ADL assays both showed good analytical performance with respect to sensitivity, specificity, linearity, and precision suitable for routine clinical use as well as excellent correlation to current commercial ELISA IFX and ADL measurement methods.</p> <p><br></p><p>Results indicated that the Procise IFX and Procise ADL assays are sensitive, specific, and precise yielding results in less than 5 minutes from either whole blood or serum. This indicates the Procise IFX and Procise ADL assays are useful for obtaining fast and accurate IFX or ADL quantitation, thus avoiding delays inherent to current methods and enabling immediate drug level dosing decisions to be made during a single patient visit.</p>

scholarly journals Therapeutic Drug Monitoring: Performance of a FRET-Based Point-Of-Care Immunoassay for the Quantitation of Infliximab and Adalimumab in Blood

2020 ◽  
Author(s):  
Edgar Ong ◽  
Ruo Huang ◽  
Richard Kirkland ◽  
Stefan Westin ◽  
Jared Salbato ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Whole Blood ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Drug Level ◽  
Analytical Performance ◽  
Quantitative Detection ◽  
Therapeutic Drug

<p>Two fast (<5 min), time-resolved fluorescence resonance energy transfer (FRET)-based immunoassays (Procise IFX™ and Procise ADL™) were developed for the quantitative detection of infliximab (IFX), adalimumab (ADL), and their respective biosimilars for use in therapeutic drug monitoring (TDM) using 20 µL of finger prick whole blood at the point-of-care or whole blood/serum in a central lab. Studies were performed to characterize analytical performance of the Procise IFX and the Procise ADL assays on the ProciseDx™ analyzer.</p> <p><br></p><p>The Procise IFX and Procise ADL assays both showed good analytical performance with respect to sensitivity, specificity, linearity, and precision suitable for routine clinical use as well as excellent correlation to current commercial ELISA IFX and ADL measurement methods.</p> <p><br></p><p>Results indicated that the Procise IFX and Procise ADL assays are sensitive, specific, and precise yielding results in less than 5 minutes from either whole blood or serum. This indicates the Procise IFX and Procise ADL assays are useful for obtaining fast and accurate IFX or ADL quantitation, thus avoiding delays inherent to current methods and enabling immediate drug level dosing decisions to be made during a single patient visit.</p>

scholarly journals Performance of a FRET-Based Point-of-Care Immunoassay for the Quantitation of Fecal Calprotectin

2021 ◽  
Author(s):  
Manisha Yadav ◽  
Michael Skinner ◽  
Rukmini Reddy ◽  
Matthew Wong ◽  
Kevin Chon ◽  
...  
Keyword(s):  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fecal Calprotectin ◽  
Analytical Performance ◽  
Quantitative Detection ◽  
Time Resolved ◽  
Collection Device ◽  
Sensitivity Specificity ◽  
A Current

<p>A fast (~5 min), time-resolved fluorescence resonance energy transfer based immunoassay (Procise FCP<a>™</a>) was developed for the point-of-care quantitative detection of fecal calprotectin (FCP) using 15 mg of fecal specimen eluted in collection fluid from the Procise Stool Collection Device™. Studies were performed to characterize analytical performance of the Procise FCP assay on the ProciseDx™ analyzer.</p><p><br></p> <p>The Procise FCP assay showed good analytical performance with respect to sensitivity, specificity, linearity, and precision suitable for routine clinical use in a point-of-care setting as well as excellent analytical agreement with a current commercial FCP measurement method.</p><p><br></p> <p>Results indicate that the Procise FCP assay is sensitive, specific, and precise yielding results in less than 5 minutes. This indicates the Procise FCP assay is useful for obtaining fast and accurate FCP quantitation, thus avoiding delays inherent to current methods and enabling immediate clinical assessment to be made during a single patient visit.</p>

scholarly journals Performance of a FRET-Based Point-of-Care Immunoassay for the Quantitation of Fecal Calprotectin

2021 ◽  
Author(s):  
Manisha Yadav ◽  
Michael Skinner ◽  
Rukmini Reddy ◽  
Matthew Wong ◽  
Kevin Chon ◽  
...  
Keyword(s):  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Fecal Calprotectin ◽  
Analytical Performance ◽  
Quantitative Detection ◽  
Time Resolved ◽  
Collection Device ◽  
Sensitivity Specificity ◽  
A Current

<p>A fast (~5 min), time-resolved fluorescence resonance energy transfer based immunoassay (Procise FCP<a>™</a>) was developed for the point-of-care quantitative detection of fecal calprotectin (FCP) using 15 mg of fecal specimen eluted in collection fluid from the Procise Stool Collection Device™. Studies were performed to characterize analytical performance of the Procise FCP assay on the ProciseDx™ analyzer.</p><p><br></p> <p>The Procise FCP assay showed good analytical performance with respect to sensitivity, specificity, linearity, and precision suitable for routine clinical use in a point-of-care setting as well as excellent analytical agreement with a current commercial FCP measurement method.</p><p><br></p> <p>Results indicate that the Procise FCP assay is sensitive, specific, and precise yielding results in less than 5 minutes. This indicates the Procise FCP assay is useful for obtaining fast and accurate FCP quantitation, thus avoiding delays inherent to current methods and enabling immediate clinical assessment to be made during a single patient visit.</p>

scholarly journals Therapeutic Drug Monitoring: Performance of a FRET-Based Point-Of-Care Immunoassay for the Quantitation of Infliximab and Adalimumab in Blood

2020 ◽  
Author(s):  
Edgar Ong ◽  
Ruo Huang ◽  
Richard Kirkland ◽  
Stefan Westin ◽  
Jared Salbato ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Drug Monitoring ◽  
Whole Blood ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Drug Level ◽  
Analytical Performance ◽  
Quantitative Detection ◽  
Therapeutic Drug

<p>Two fast (<5 min), time-resolved fluorescence resonance energy transfer (FRET)-based immunoassays (Procise IFX™ and Procise ADL™) were developed for the quantitative detection of infliximab (IFX), adalimumab (ADL), and their respective biosimilars for use in therapeutic drug monitoring (TDM) using 20 µL of finger prick whole blood at the point-of-care or whole blood/serum in a central lab. Studies were performed to characterize analytical performance of the Procise IFX and the Procise ADL assays on the ProciseDx™ analyzer.</p> <p><br></p><p>The Procise IFX and Procise ADL assays both showed good analytical performance with respect to sensitivity, specificity, linearity, and precision suitable for routine clinical use as well as excellent correlation to current commercial ELISA IFX and ADL measurement methods.</p> <p><br></p><p>Results indicated that the Procise IFX and Procise ADL assays are sensitive, specific, and precise yielding results in less than 5 minutes from either whole blood or serum. This indicates the Procise IFX and Procise ADL assays are useful for obtaining fast and accurate IFX or ADL quantitation, thus avoiding delays inherent to current methods and enabling immediate drug level dosing decisions to be made during a single patient visit.</p>

scholarly journals A ruthenium-based catalyst on carbon electrodes for electrochemical water splitting

2021 ◽  
Author(s):  
Lin Li ◽  
Biswanath Das ◽  
Ahibur Rahaman ◽  
Andrey Shatskiy ◽  
Fei Ye ◽  
...  
Keyword(s):  
Water Splitting ◽  
Water Oxidation ◽  
State Of The Art ◽  
Cost Effective ◽  
Molecular Catalyst ◽  
Electrolysis Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Energy Economy ◽  
Electrochemical Water Splitting ◽  
Walled Carbon Nanotubes

Electrochemical water splitting constitutes one of the most promising strategies for converting water into hydrogen-based fuels, and this technology is predicted to play a key role in our transition towards a carbon-neutral energy economy. To enable the design of cost-effective electrolysis cells based on this technology, new and more efficient anodes with augmented water splitting activity and stability will be required. Herein, we report an active molecular Ru-based catalyst for electrochemically-driven water oxidation and two simple methods for preparing anodes by attaching this catalyst onto multi-walled carbon nanotubes. The anodes modified with the molecular catalyst were characterized by a broad toolbox of microscopy and spectroscope techniques, and interestingly no RuO2 formation was detected during electrocatalysis over 4 h. These results demonstrate that the herein presented strategy can be used to prepare anodes that rival the performance of state-of-the-art metal oxide anodes.

scholarly journals Theoretical Modeling of Viscosity Monitoring with Vibrating Resonance Energy Transfer for Point-of-Care and Environmental Monitoring Applications

Micromachines ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 3 ◽  
Author(s):  
Gorkem Memisoglu ◽  
Burhan Gulbahar ◽  
Joseba Zubia ◽  
Joel Villatoro
Keyword(s):  
Energy Transfer ◽  
Environmental Monitoring ◽  
System Design ◽  
Point Of Care ◽  
Resonance Energy Transfer ◽  
Microfluidic Channel ◽  
Resonance Energy ◽  
Polluted Water ◽  
Hardware Complexity ◽  
Monitoring Applications

Förster resonance energy transfer (FRET) between two molecules in nanoscale distances is utilized in significant number of applications including biological and chemical applications, monitoring cellular activities, sensors, wireless communications and recently in nanoscale microfluidic radar design denoted by the vibrating FRET (VFRET) exploiting hybrid resonating graphene membrane and FRET design. In this article, a low hardware complexity and novel microfluidic viscosity monitoring system architecture is presented by exploiting VFRET in a novel microfluidic system design. The donor molecules in a microfluidic channel are acoustically vibrated resulting in VFRET in the case of nearby acceptor molecules detected with their periodic optical emission signals. VFRET does not require complicated hardware by directly utilizing molecular interactions detected with the conventional photodetectors. The proposed viscosity measurement system design is theoretically modeled and numerically simulated while the experimental challenges are discussed. It promises point-of-care and environmental monitoring applications including viscosity characterization of blood or polluted water.

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