scholarly journals Does methylation of NFATC1 and C-FOS genes are associated with post-menopausal osteoporosis?

2020 ◽  
Author(s):  
Rasime Kalkan ◽  
Özgür Tosun
Keyword(s):  
Postmenopausal Women ◽  
Epigenetic Regulation ◽  
Methylation Status ◽  
Menopausal Status ◽  
Epigenetic Factors ◽  
Post Menopausal Osteoporosis ◽  
Menopausal Women ◽  
Post Menopausal ◽  
First Time ◽  
Menopausal Osteoporosis

ABSTRACTGenetic and epigenetic factors have an important role during the development of osteoporosis. RANK/RANKL pathway is important for the bone remodeling and NFATC1 and c-FOS are the downtargets of this pathway. Here, we report methylation status of NFATC1 and C-FOS genes in post and premenopausal cases. In this study 30 pre-menopausal and 35 post-menopausal cases were included. MS-HRM was used for identification of NFATC1 and C-FOS metyhylation.NFATC1 were methylated in 11 of the 35 post-menopausal women and C-FOS were methylated in 6 of the postmenopausal women (p >0.005). Here, we found statistically significant association between unmethylation of NFATC1 and post-menopausal status. This result explains the epigenetic regulation of osteoclasts during the menopausal transition and our results can be used for epigenetic explanation of post-menopausal osteoporosis for the first time in the literature. Although the limited number of studies in this field makes our results crucial. Therefore, our results showed great value of epigenetic profile of post-menopausal women.

scholarly journals Do we use methylation of NFATC1 and FOS genes as a biomarker for postmenopausal osteoporosis?

2020 ◽  
Vol 23 (2) ◽  
pp. 35-40
Author(s):  
R Kalkan ◽  
O Tosun
Keyword(s):  
Postmenopausal Women ◽  
Postmenopausal Osteoporosis ◽  
High Resolution Melting ◽  
Methylation Status ◽  
Premenopausal Women ◽  
Epigenetic Factors ◽  
Receptor Activator ◽  
Postmenopausal Status ◽  
First Time ◽  
Nuclear Factor Κ B

Abstract Genetic and epigenetic factors have an important role during the development of osteoporosis. Receptor activator of nuclear factor-κ B (NF-κB) (RANK)/receptor activator of NF-κB ligand (RANKL) pathway is important for the bone remodeling, and NFATC1 and FOS are the downtargets of this pathway. Here, we report methylation status of NFATC1 and FOS genes in post- and premenopausal women. In this study, 30 premenopausal and 35 postmenopausal women were included. Methylation sensitive-high resolution melting (MS-HRM) analysis was used for identification of NFATC1 and FOS genes methylation. The NFATC1 gene was methylated in 11 of the 35 postmenopausal women, and the FOS gene was methylated in six of the postmenopausal women (p >0.005). Here, we found statistically significant association between unmethylation of the NFATC1 gene and postmenopausal status. This result explains the epigenetic regulation of osteoclasts during the menopausal transition, and for the first time, our results can be used for epigenetic explanation of postmenopausal osteoporosis in the literature. However, the limited number of studies in this field makes our results crucial. Our results showed great value of epigenetic profiles of postmenopausal women.

scholarly journals Expression of circulating Mir-139-5p is associated with Postmenopausal Osteoporosis in Indian women

2021 ◽  
Vol 10 (4) ◽  
pp. 102-105
Author(s):  
Rupakala BM ◽  
K Anbazhaganb ◽  
S Prabhakarac ◽  
MPA Sailakshmia
Keyword(s):  
Bone Metabolism ◽  
Therapeutic Strategies ◽  
Circulating Mirnas ◽  
Biochemical Tests ◽  
Indian Women ◽  
Post Menopausal Osteoporosis ◽  
Menopausal Women ◽  
Control Bone ◽  
Post Menopausal ◽  
Menopausal Osteoporosis

Background: Osteoporosis in post-menopausal women is known to progress periodically and is highly associated with inflammation. MicroRNAs regulate inflammatory process, which may therefore control bone metabolism. Deregulation of miRNAs associated with inflammation may lead to development and progression of osteoporosis. We selected four miRNAs known to be involved in inflammation to test their association with post-menopausal osteoporosis. Methods: We quantified four circulating miRNAs, hsa-miR-139-5p, hsa-miR-342-3p, hsa-miR-146a and hsa-miR-24-3p in plasma samples of 25 post-menopausal osteoporosis and 25 post-menopausal healthy subjects. Related biochemical tests were done using Cobas e411 and ELISA. Results: Upon quantification of circulating miRNAs, we observed that hsa-miR-139-5p was expressed higher in post-menopausal osteoporotic samples (p=0.01). The expression of hsa-miR-24-3p was seen lower in osteoporotic samples though not highly significant (p=0.2). Conclusion: Differential expression of hsa-miR-139-5p and hsa-miR-24-3p was seen in osteoporosis subjects. These miRNA could be significantly involved in development and progression of osteoporosis. Further studies are required to highlight miRNAs’ involvement in regulating bone metabolism, which could be manipulated to use them as marker or therapeutic strategies to alleviate osteoporosis.

scholarly journals Effect of single dose intravenous zoledronic acid on bone mineral density in post-menopausal osteoporosis of Bangladeshi women

1970 ◽  
Vol 36 (3) ◽  
pp. 74-77
Author(s):  
M Hossain ◽  
IH Chowdhury ◽  
MA Emran ◽  
AHMA Habib ◽  
AKM Asaduzzaman ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Zoledronic Acid ◽  
Dual Energy ◽  
Mineral Density ◽  
X Ray ◽  
T Score ◽  
Post Menopausal Osteoporosis ◽  
Menopausal Women ◽  
Post Menopausal ◽  
Menopausal Osteoporosis

The osteoporosis is a major health threat that affects every third post-menopausal women. Postmenopausal osteoporosis is complicated with vertebral, femoral or radius fracture. This prospective study on post-menopausal osteoporosis was carried out in the Pain Centre, Department of Anaesthesia, Analgesia and Intensive Care Medicine of BSMMU, Dhaka during the period of January 2008 to January 2010. The post-menopausal women with back pain were screened by spinal radiographs and dual-energy X-ray absorptiometry (DXA) of lumbar spine to determine the bone mineral density (BMD). The woman after menopause with a BMD T-score of -2.5 or less with or without evidence of vertebral fracture is considered as post-menopausal osteoporosis. A total of 55 post-menopausal osteoporotic patients were assigned to receive a single dose of IV infusion of zoledronic acid (5mg) along with dietary calcium and vitamin-D. The spinal radiographs and dual-energy X-ray absorptiometry (DXA) were repeated in all the 55 patients at 12 months following zoledronic acid infusion. The mean BMD of lumbar spine increased significantly from pre-infusion value of 0.75695g/cm2 to post-infusion of 0.80216 g/cm2. The T-score also increased from pre infusion value of -3.567±0.77 to -3.158±0.08 in 12 months following the infusion (P<0.01). The increase is 5.026% higher than pre infusion values. The spinal radiographs taken before infusion of zoledronic acid, showed 14 fractures. There was no new fracture in any case during the 12 months study period. So, it can be concluded that once yearly IV infusion of zoledronic acid is associated with a significant increase in BMD and decrease in the risk of vertebral fracture. DOI: 10.3329/bmrcb.v36i3.7285Bangladesh Med Res Counc Bull 2010; 36: 74 -77

Efficacy of Denosumab on bone metabolism after a 12-month treatment, in women with severe post-menopausal osteoporosis

2014 ◽  
Author(s):  
Renato Pastore ◽  
Patrizio Pasqualetti ◽  
Laura Chioma ◽  
Giuseppe Vancieri ◽  
Simona Frontoni
Keyword(s):  
Bone Metabolism ◽  
Post Menopausal Osteoporosis ◽  
Post Menopausal ◽  
Menopausal Osteoporosis

Medicinal Plants for the Management of Post Menopausal Osteoporosis: A Review~!2009-08-29~!2009-11-14~!2010-03-05~!

2010 ◽  
Vol 2 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Annie Shirwaikar ◽  
Saleemulla Khan ◽  
Yogesh H. Kamariya ◽  
Bharatkumar D. Patel ◽  
Falguni P. Gajera
Keyword(s):  
Medicinal Plants ◽  
Post Menopausal Osteoporosis ◽  
Post Menopausal ◽  
Menopausal Osteoporosis

scholarly journals Endogenous hormones and risk of invasive breast cancer in pre- and post-menopausal women: findings from the UK Biobank

2021 ◽  
Author(s):  
Sandar Tin Tin ◽  
Gillian K. Reeves ◽  
Timothy J. Key
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Invasive Breast Cancer ◽  
Cox Regression ◽  
Menopausal Status ◽  
Endogenous Hormones ◽  
Uk Biobank ◽  
Menopausal Women ◽  
Post Menopausal

Abstract Background Some endogenous hormones have been associated with breast cancer risk, but the nature of these relationships is not fully understood. Methods UK Biobank was used. Hormone concentrations were measured in serum collected in 2006–2010, and in a repeat subsample (N ~ 5000) in 2012–13. Incident cancers were identified through data linkage. Cox regression models were used, and hazard ratios (HRs) corrected for regression dilution bias. Results Among 30,565 pre-menopausal and 133,294 post-menopausal women, 527 and 2,997, respectively, were diagnosed with invasive breast cancer during a median follow-up of 7.1 years. Cancer risk was positively associated with testosterone in post-menopausal women (HR per 0.5 nmol/L increment: 1.18; 95% CI: 1.14, 1.23) but not in pre-menopausal women (pheterogeneity = 0.03), and with IGF-1 (insulin-like growth factor-1) (HR per 5 nmol/L increment: 1.18; 1.02, 1.35 (pre-menopausal) and 1.07; 1.01, 1.12 (post-menopausal); pheterogeneity = 0.2), and inversely associated with SHBG (sex hormone-binding globulin) (HR per 30 nmol/L increment: 0.96; 0.79, 1.15 (pre-menopausal) and 0.89; 0.84, 0.94 (post-menopausal); pheterogeneity = 0.4). Oestradiol, assessed only in pre-menopausal women, was not associated with risk, but there were study limitations for this hormone. Conclusions This study confirms associations of testosterone, IGF-1 and SHBG with breast cancer risk, with heterogeneity by menopausal status for testosterone.

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