scholarly journals Recombinant Expression of Metal-Binding Proteins (Bacterioferritin, MBP, Metallothionein, and OprF) and Pesticide-Binding Proteins (LC-Cutinase and OpdA) in Escherichia coli for Detection of Agricultural Contaminants

2020 ◽  
Author(s):  
Kimberly Hwang ◽  
Allison Kuo ◽  
Eugene Choi ◽  
Jessica Chen ◽  
Jonathan Hsu ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Fruits And Vegetables ◽  
Binding Proteins ◽  
Recombinant Expression ◽  
Agricultural Residues ◽  
Agricultural Residue ◽  
The Public ◽  
Residue Detection ◽  
Agricultural Contaminants

AbstractWe consume fruits and vegetables every day without knowing whether or not agricultural residues (i.e. pesticides & heavy metals) are present on them. Current methods of agricultural residue detection are not easily accessible to the public and are inconvenient for everyday use. Thus, we want to develop a convenient visualization of agricultural residues by designing metal-binding and pesticide-binding proteins along with colored proteins that can directly interact with these residues. We worked with metal-binding proteins including Bacterioferritin, MBP, Metallothionein, and OprF. We also utilized pesticide-binding proteins LC Cutinase and OpdA. In this paper, we envision a system in which our residue-binding proteins are fused with chromoproteins to allow for the visible detection of agricultural residues on produce. Our final product can be used by consumers, distributors and farmers alike.

Heavy metal-binding proteins from metal-stimulated bacteria as a novel adsorbent for metal removal technology

2006 ◽  
Vol 53 (6) ◽  
pp. 221-226 ◽  
Author(s):  
D. Sano ◽  
K. Myojo ◽  
T. Omura
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Activated Sludge ◽  
Metal Binding ◽  
Binding Proteins ◽  
Metal Removal ◽  
Heavy Metal Removal ◽  
Copper Ion ◽  
Amino Acid Sequences ◽  
Heavy Metal Binding

Water pollution with toxic heavy metals is of growing concern because heavy metals could bring about serious problems for not only ecosystems in the water environment but also human health. Some metal removal technologies have been in practical use, but much energy and troublesome treatments for chemical wastes are required to operate these conventional technologies. In this study, heavy metal-binding proteins (HMBPs) were obtained from metal-stimulated activated sludge culture with affinity chromatography using copper ion as a ligand. Two-dimensional electrophoresis revealed that a number of proteins in activated sludge culture were recovered as HMBPs for copper ion. N-termini of five HMBPs were determined, and two of them were found to be newly discovered proteins for which no amino acid sequences in protein databases were retrieved at more than 80% identities. Metal-coordinating amino acids occupied 38% of residues in one of the N-terminal sequences of the newly discovered HMBPs. Since these HMBPs were expected to be stable under conditions of water and wastewater treatments, it would be possible to utilize HMBPs as novel adsorbents for heavy metal removal if mass volume of HMBPs can be obtained with protein cloning techniques.

Induction of Metallothionein

1989 ◽  
Vol 8 (7) ◽  
pp. 1315-1321 ◽  
Author(s):  
Curtis D. Klaassen ◽  
Lois D. Lehman-Mckeeman
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Molecular Mechanism ◽  
Organic Compounds ◽  
Metal Binding ◽  
Binding Proteins ◽  
Biological Function ◽  
Metal Homeostasis ◽  
Pivotal Role ◽  
The Relationship

Metallothioneins (MTs) are cysteine-rich metal-binding proteins. These proteins play a pivotal role in heavy metal homeostasis and have been widely studied by biochemists, toxicologists, nutritional scientists, and molecular biologists. It is well established that MTs are inducible proteins. They are normally synthesized at low basal levels, but exposure to a wide variety of heavy metals and many organic compounds will dramatically increase synthesis of MTs. This paper summarizes MT induction by reviewing the chemicals that induce these proteins, the molecular mechanism involved in this induction, and the relationship between MT induction and biological function.

Functional metal-binding proteins by metal-stimulated bacteria for the development of an innovative metal removal technology

2003 ◽  
Vol 47 (10) ◽  
pp. 109-115 ◽  
Author(s):  
T. Antsuki ◽  
D. Sano ◽  
T. Omura
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Activated Sludge ◽  
Metal Binding ◽  
Binding Proteins ◽  
Metal Removal ◽  
Copper Ion ◽  
Growth Media ◽  
Affinity Column ◽  
Removal Technology

Heavy metal pollution has become an environmental problem throughout the world because heavy metals can be accumulated into the food chain and bring about serious problems, not only for ecosystems but also for human health. In this study, functional metal-binding proteins (FMBPs) were isolated from a metal-stimulated activated sludge culture with the aim of applying them to an innovative metal removal technology. Activated sludge bacteria was cultured in growth media including copper ion, and the stimulation of protein production by copper ion led to the 14% increase in a quantity of extracted crude proteins per 1 g of bacterial cell pellet (wet). In order to isolate FMBPs, extracted crude proteins were applied to the immobilized metal affinity column in which each of copper, nickel and zinc was used as a ligand. Several FMBPs were succesfully isolated from copper-stimulated bacteria. One of FMBPs (molecular weight of about 40 kDa) exhibited an ability to adsorb all three metals. The multi metal-binding property of this FMBP could be applied to an innovative metal removal technology. Furthermore, isolated FMBPs that could capture only one kind of heavy metal would also be attractive as a metal adsorbent in recovering a specific metal as a resource from wastewater, including several heavy metals.

Metal-Binding Proteins and Peptides for the Detection of Heavy Metals in Aquatic Organisms

2018 ◽  
pp. 239-254 ◽  
Author(s):  
David H. Petering ◽  
Mark Goodrich ◽  
William Hodgman ◽  
Susan Krezoski ◽  
Daniel Weber ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Metal Binding ◽  
Binding Proteins ◽  
Aquatic Organisms ◽  
Proteins And Peptides

Profile of metal-binding proteins and heme oxygenase in red carp treated with heavy metals, pesticides and surfactants

1990 ◽  
Vol 44 (4) ◽  
pp. 643-649 ◽  
Author(s):  
Toshihiko Ariyoshi ◽  
Seiichi Shiiba ◽  
Hiroyuki Hasegawa ◽  
Koji Arizono
Keyword(s):  
Heavy Metals ◽  
Heme Oxygenase ◽  
Metal Binding ◽  
Binding Proteins

scholarly journals Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Seyed Mohammad Davachi ◽  
Neethu Pottackal ◽  
Hooman Torabi ◽  
Alireza Abbaspourrad
Keyword(s):  
Fruits And Vegetables ◽  
Uv Light ◽  
Barrier Properties ◽  
Edible Films ◽  
Food Preservation ◽  
Elongation At Break ◽  
The Public ◽  
Medical Benefits ◽  
Seed Mucilage

AbstractThere is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome. With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil. These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits. All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food. Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films. These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage. Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability. This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.

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