scholarly journals SpheroidPicker: An Automated 3D cell culture manipulator robot using deep learning

2020 ◽  
Author(s):  
Istvan Grexa ◽  
Akos Diosdi ◽  
Andras Kriston ◽  
Nikita Moshkov ◽  
Maria Harmati ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
High Throughput Screening ◽  
Ex Vivo ◽  
Low Cost ◽  
3D Cell Culture ◽  
Experimental Conditions ◽  
The Past ◽  
3D Cell Cultures ◽  
Manipulator Robot

AbstractRecent statistics report that more than 3.7 million new cases of cancer occur in Europe yearly, and the disease accounts for approximately 20 % of all deaths. High-throughput screening of cancer cell cultures has dominated the search for novel, effective anticancer therapies in the past decades. Recently, ex vivo 3D cell cultures from the patient’s own cancer cells have gained importance. We recently evaluated the major advancements and needs of the 3D cell cultures screening field, and we concluded that strictly standardized sample preparation is the most desired development. Here we propose an artificial intelligence-guided low-cost 3D cell culture delivery system. It consists of a light microscope, a micromanipulator, a syringe pump, and a controller computer. The system performs morphology-based feature analysis on spheroids and transfers the most appropriate ones between various sample holders. It can select the samples from standard sample holders, including Petri dishes and microwell plates, and then transfer them to a variety of holders up to 384 well plates. The device performs reliable semi- and fully automated spheroid transfer. This results in highly controlled experimental conditions and eliminates non-trivial side effects of sample variability that is a key aspect towards next-generation precision medicine.

scholarly journals SpheroidPicker for automated 3D cell culture manipulation using deep learning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Istvan Grexa ◽  
Akos Diosdi ◽  
Maria Harmati ◽  
Andras Kriston ◽  
Nikita Moshkov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Precision Medicine ◽  
High Throughput Screening ◽  
Standard Sample ◽  
Ex Vivo ◽  
Low Cost ◽  
3D Cell Culture ◽  
Syringe Pump ◽  
Experimental Conditions ◽  
The Past

AbstractRecent statistics report that more than 3.7 million new cases of cancer occur in Europe yearly, and the disease accounts for approximately 20% of all deaths. High-throughput screening of cancer cell cultures has dominated the search for novel, effective anticancer therapies in the past decades. Recently, functional assays with patient-derived ex vivo 3D cell culture have gained importance for drug discovery and precision medicine. We recently evaluated the major advancements and needs for the 3D cell culture screening, and concluded that strictly standardized and robust sample preparation is the most desired development. Here we propose an artificial intelligence-guided low-cost 3D cell culture delivery system. It consists of a light microscope, a micromanipulator, a syringe pump, and a controller computer. The system performs morphology-based feature analysis on spheroids and can select uniform sized or shaped spheroids to transfer them between various sample holders. It can select the samples from standard sample holders, including Petri dishes and microwell plates, and then transfer them to a variety of holders up to 384 well plates. The device performs reliable semi- and fully automated spheroid transfer. This results in highly controlled experimental conditions and eliminates non-trivial side effects of sample variability that is a key aspect towards next-generation precision medicine.

Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

2020 ◽  
Vol 25 (3) ◽  
pp. 234-246
Author(s):  
Charles McRae White ◽  
Mark A. Haidekker ◽  
William S. Kisaalita
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Cell Biology ◽  
Low Cost ◽  
3D Cell Culture ◽  
Biomechanical Properties ◽  
Practical Applications ◽  
3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

scholarly journals Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Nina Compera ◽  
Scott Atwell ◽  
Johannes Wirth ◽  
Bernhard Wolfrum ◽  
Matthias Meier
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Large Scale ◽  
3D Cell Culture ◽  
3D Cell Cultures ◽  
Culture Formation ◽  
Large Scale Integration ◽  
3D Printed ◽  
On Chip ◽  
Scale Integration

For integration of 3D cell cultures on microfluidic large-scale integration chips, we upscaled pneumatic membrane valves using 3D-printed replica molds. Unit cell operations for 3D cell culture formation, culture, retrieval, and fusion are designed.

scholarly journals Simplified One-stop Protocols to Extract Collagen from Various Animal Tissues for 3D Cell Cultures

2020 ◽  
Author(s):  
John A. Terrell ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
3D Cell Culture ◽  
Animal Tissues ◽  
3D Cell Cultures ◽  
One Stop

In this manuscript, we present a one-stop protocol to extract collagen from various animal tissues, and apply it to fabricate 3D cell culture scaffolds.

An antioxidant self-healing hydrogel for 3D cell cultures

2020 ◽  
Vol 8 (7) ◽  
pp. 1383-1388 ◽  
Author(s):  
Lei Yang ◽  
Yuan Zeng ◽  
Haibo Wu ◽  
Chunwu Zhou ◽  
Lei Tao
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Biginelli Reaction ◽  
3D Cell Culture ◽  
Self Healing ◽  
3D Cell Cultures

An antioxidant self-healing hydrogel has been facilely prepared using the Biginelli reaction and then used for 3D cell culture.

Fluorination on polyethylenimine allows efficient 2D and 3D cell culture gene delivery

2015 ◽  
Vol 3 (4) ◽  
pp. 642-650 ◽  
Author(s):  
Jia Lv ◽  
Hong Chang ◽  
Yu Wang ◽  
Mingming Wang ◽  
Jianru Xiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Gene Delivery ◽  
Cell Cultures ◽  
Gene Transfection ◽  
3D Cell Culture ◽  
Transfection Efficacy ◽  
3D Cell Cultures ◽  
2D And 3D

Fluorinationviaanhydride and oxirane reactions enhances the gene transfection efficacy of PEI on 3D cell cultures.

scholarly journals Simplified One-stop Protocols to Extract Collagen from Various Animal Tissues for 3D Cell Cultures

2020 ◽  
Author(s):  
John A. Terrell ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
3D Cell Culture ◽  
Animal Tissues ◽  
3D Cell Cultures ◽  
One Stop

In this manuscript, we present a one-stop protocol to extract collagen from various animal tissues, and apply it to fabricate 3D cell culture scaffolds.

scholarly journals A Modular Toolkit to Fabricate Microfluidic Devices for 3D Cell Culture and Near Real-time Measurements

2020 ◽  
Author(s):  
Giraso Kabandana ◽  
Adam Michael Ratajczak ◽  
Chengpeng Chen
Keyword(s):  
Cell Culture ◽  
Real Time ◽  
Low Cost ◽  
New Technology ◽  
Microfluidic Channel ◽  
3D Cell Culture ◽  
Microfluidic Devices ◽  
In Vitro Cell Cultures ◽  
Scoring Tools

Microfluidic technology has tremendously facilitated the development of in vitro cell cultures and studies. Conventionally, microfluidic devices are fabricated with extensive facilities by well-trained researchers, which hinders the widespread adoption of the technology for broader applications. Enlightened by the fact that low-cost microbore tubing is a natural microfluidic channel, we developed a series of adaptors in a toolkit that can twine, connect, organize, and configure the tubing to produce functional microfluidic units. Three subsets of the toolkit were thoroughly developed: the tubing and scoring tools, the flow adaptors, and the 3D cell culture suite. To demonstrate the usefulness and versatility of the toolkit, we assembled a microfluidic device and successfully applied it for 3D macrophage cultures, flow-based stimulation, and automated near real-time quantitation with new knowledge generated. Overall, we present a new technology that allows simple, fast, and robust assembly of customizable and scalable microfluidic devices with minimal facilities, which is broadly applicable to research that needs or could be enhanced by microfluidics.

scholarly journals Pancreatic Ductal Adenocarcinoma: Preclinical in vitro and ex vivo Models

2021 ◽  
Vol 9 ◽  
Author(s):  
Beate Gündel ◽  
Xinyuan Liu ◽  
Matthias Löhr ◽  
Rainer Heuchel
Keyword(s):  
Pancreatic Ductal Adenocarcinoma ◽  
Ex Vivo ◽  
Treatment Options ◽  
3D Cell Culture ◽  
Therapy Resistance ◽  
Ductal Adenocarcinoma ◽  
The Past ◽  
Slow Progress

Pancreatic ductal adenocarcinoma (PDAC) is one of the most overlooked cancers despite its dismal median survival time of 6 months. The biggest challenges in improving patient survival are late diagnosis due to lack of diagnostic markers, and limited treatment options due to almost complete therapy resistance. The past decades of research identified the dense stroma and the complex interplay/crosstalk between the cancer- and the different stromal cells as the main culprits for the slow progress in improving patient outcome. For better ex vivo simulation of this complex tumor microenvironment the models used in PDAC research likewise need to become more diverse. Depending on the focus of the investigation, several in vitro and in vivo models for PDAC have been established in the past years. Particularly, 3D cell culture such as spheroids and organoids have become more frequently used. This review aims to examine current PDAC in vitro models, their inherent limitations, and their successful implementations in research.

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