scholarly journals Evaluation of a Recessive Male Floret Color in Cultivated Northern Wild Rice (Zizania palustris L.) for Pollen-Mediated Gene Flow Studies

2021 ◽  
Author(s):  
Clare Gietzel ◽  
Jacques Duquette ◽  
Lillian McGilp ◽  
Jennifer Kimball

AbstractNorthern wild rice (NWR; Zizania palustris L.) is a wind-pollinated, annual, aquatic grass that grows naturally in the Great Lakes Region (GLR) of the United States and Canada, and is also cultivated in flooded paddies, predominantly in California and Minnesota. A better understanding of pollen-mediated gene flow is needed within the species for both conservation and breeding efforts as cultivation occurs within the species natural range and spatially-isolated, paddy structures are limited within breeding programs. Widely cited pollen travel research in NWR demonstrated that pollen could travel at least 3200m. However, a population segregating for male sterility was used as the pollen recipient in the study and was determined to not be adequate for NWR pollen travel studies. Here, we present the characterization of a recessive white male floret (WMF) population in contrast to the dominant, purple male floret (PMF) color of cultivated NWR along with estimates of pollen-mediated gene flow in a cultivated paddy setting. Studies conducted in 2018 and 2019 revealed that the primary amount of pollen-mediated gene flow occurred within the first 7m from the PMF donor source with no gene flow detected past 63m. These results suggest that the likelihood of pollen-mediated gene flow between cultivated NWR and natural stands remains low. We also identified a strong linkage between male floret, auricle, and culm color. This study demonstrates that the WMF trait is an excellent candidate for use in pollen-mediated gene flow studies in NWR.

2019 ◽  
Vol 31 ◽  
Author(s):  
David Dean Biesboer

Abstract Wild rice is an enigmatic plant species in the upper northern Midwestern U.S. Wild rice (Zizania palustris L.) is widespread in the region. However, it is both a wild species and a semi-domesticated crop. It is enigmatic because it is grown and harvested by machine in large paddies by commercial farmers but wild populations are considered to be important spiritually to native Americans in the United States. Native Americans and the public harvest wild rice by hand and sell and trade it on a local basis. This has caused a political controversy between people who consider wild rice to be a crop belonging only to native Indian bands and those who consider that it belongs to citizens of the U.S. Conservation of populations of wild rice has become politically difficult because of these contrasting views. This paper discusses this controversy and addresses the difficulty of conserving this economically widespread plant that is under ecological pressure in modern times.


2021 ◽  
Author(s):  
Matthew Haas ◽  
Thomas Kono ◽  
Marissa Macchietto ◽  
Reneth Millas ◽  
Lillian McGilp ◽  
...  

ABSTRACTNorthern Wild Rice (NWR; Zizania palustris L.) is an aquatic grass native to North America that is notable for its nutritious grain. This is an important species with ecological, cultural, and agricultural significance, specifically in the Great Lakes region of the United States. Using long- and short-range sequencing, Hi-C scaffolding, and RNA-seq data from eight tissues, we generated an annotated whole genome de novo assembly of NWR. The assembly is 1.29 Gb, highly repetitive (∼76.0%), and contains 46,421 putative protein-coding genes. The expansion of retrotransposons within the genome and a whole genome duplication prior to the Zizania-Oryza speciation event have both led to an increase in genome size of NWR in comparison with O. sativa and Z. latifolia. Both events depict a genome rapidly undergoing change over a short evolutionary time. Comparative analyses revealed conservation of large syntenic blocks with Oryza sativa L., which were used to identify putative seed shattering genes. Estimates of divergence times revealed the Zizania genus diverged from Oryza ∼26-30 million years ago (MYA), while NWR and Zizania latifolia diverged from one another ∼6-8 MYA. Comparative genomics confirmed evidence of a whole genome duplication in the Zizania genus and provided support that the event was prior to the NWR-Z. latifolia speciation event. This high-quality genome assembly and annotation provides a valuable resource for comparative genomics in the Oryzeae tribe and provides an important resource for future conservation and breeding efforts of NWR.


1993 ◽  
Vol 89 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Douglas G. Muench ◽  
O. William Archibold ◽  
Allen G. Good

Author(s):  
Brianna R. Cornelius

Although a notable body of work has emerged describing gay male speech (GMS), its overlap with African American language (AAL) remains comparatively understudied. This chapter explores the assumption of whiteness that has informed research on gay identity and precluded intersectional considerations in sociolinguistic research. Examining the importance of racial identity, particularly Blackness, to the construction of gay identity in the United States, the chapter investigates the treatment of GMS as white by default, with the voices of gay men of color considered additive. The desire vs. identity debate in language and sexuality studies contributed to an understanding of gay identity as community-based practice, thereby laying a necessary framework for the study of GMS. However, this framework led to a virtually exclusive focus on white men’s language use. Although efforts to bring a community-based understanding to gay identity have been groundbreaking, the lack of consideration of intersectionality has erased contributions to GMS from racially based language varieties, such as AAL.


2021 ◽  
Vol 31 (1-2) ◽  
pp. 7-28
Author(s):  
April L. Peters ◽  
Angel Miles Nash

The rallying, clarion call to #SayHerName has prompted the United States to intentionally include the lives, voices, struggles, and contributions of Black women and countless others of her ilk who have suffered and strived in the midst of anti-Black racism. To advance a leadership framework that is rooted in the historicity of brilliance embodied in Black women’s educational leadership, and their proclivity for resisting oppression, we expand on intersectional leadership. We develop this expansion along three dimensions of research centering Black women’s leadership: the historical foundation of Black women’s leadership in schools and communities, the epistemological basis of Black women’s racialized and gendered experiences, and the ontological characterization of Black women’s expertise in resisting anti-Black racism in educational settings. We conclude with a four tenet articulation detailing how intersectional leadership: (a) is explicitly anti-racist; (b) is explicitly anti-sexist; (c) explicitly acknowledges the multiplicative influences of marginalization centering race and gender, and across planes of identity; and (d) explicitly leverages authority to serve and protect historically underserved communities.


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