scholarly journals Arginine induced Streptococcus gordonii biofilm detachment using a novel rotating-disc rheometry method

2021 ◽  
Author(s):  
Erin S. Gloag ◽  
Daniel J. Wozniak ◽  
Kevin L. Wolf ◽  
James G. Masters ◽  
Carlo Amorin Daep ◽  
...  
Keyword(s):  
Dental Plaque ◽  
Oral Disease ◽  
Shear Stresses ◽  
Streptococcus Gordonii ◽  
Oral Diseases ◽  
Rotating Disc ◽  
Highly Sensitive ◽  
Biofilm Detachment ◽  
Biofilm Structure ◽  
Plaque Biofilm

AbstractOral diseases are one of the most common pathologies affecting human health. These diseases are typically associated with dental plaque-biofilms, through either build-up of the biofilm or dysbiosis of the microbial community. Arginine can disrupt dental plaque-biofilms, and maintain plaque homeostasis, making it an ideal therapeutic to combat the development of oral disease. Despite our understanding of the actions of arginine towards dental plaque-biofilms, it is still unclear how or if arginine effects the mechanical integrity of the dental plaque-biofilm. Here we adapted a rotating-disc rheometry assay, which is routinely used in marine microbial ecology, to study how arginine treatment of Streptococcus gordonii biofilms influences biofilm detachment from surfaces. We demonstrate that the assay is highly sensitive at quantifying the presence of biofilm and the detachment or rearrangement of the biofilm structure as a function of shear stress. We demonstrate that arginine treatment leads to earlier detachment of the biofilm, indicating that arginine treatment weakens the biofilm, making it more suspectable to removal by shear stresses. Our results add to the understanding that arginine targets biofilms by multifaceted mechanisms, both metabolic and physical, further promoting the potential of arginine as an active compound in dentifrices to maintain oral health.

scholarly journals Interspecies Signaling between Veillonella atypica and Streptococcus gordonii Requires the Transcription Factor CcpA

2009 ◽  
Vol 191 (17) ◽  
pp. 5563-5565 ◽  
Author(s):  
Bart P. Johnson ◽  
Benjamin J. Jensen ◽  
Eric M. Ransom ◽  
Kimberly A. Heinemann ◽  
Kelley M. Vannatta ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Dental Plaque ◽  
Streptococcus Gordonii ◽  
Interspecies Interaction ◽  
Plaque Biofilm

ABSTRACT Streptococcus gordonii and Veillonella atypica, two early-colonizing members of the dental plaque biofilm, participate in a relationship that results in increased transcription of the S. gordonii gene amyB, encoding an α-amylase. We show that the transcription factor CcpA is required for this interspecies interaction.

scholarly journals Understanding the Matrix: The Role of Extracellular DNA in Oral Biofilms

2021 ◽  
Vol 2 ◽  
Author(s):  
Hannah J. Serrage ◽  
Mark A. Jepson ◽  
Nadia Rostami ◽  
Nicholas S. Jakubovics ◽  
Angela H. Nobbs
Keyword(s):  
Dental Plaque ◽  
Molecular Mechanisms ◽  
Structural Integrity ◽  
Extracellular Dna ◽  
Oral Disease ◽  
Diverse Range ◽  
Oral Biofilms ◽  
The Matrix ◽  
Plaque Biofilm

Dental plaque is the key etiological agent in caries formation and the development of the prevalent chronic oral inflammatory disease, periodontitis. The dental plaque biofilm comprises a diverse range of microbial species encased within a rich extracellular matrix, of which extracellular DNA (eDNA) has been identified as an important component. The molecular mechanisms of eDNA release and the structure of eDNA have yet to be fully characterized. Nonetheless, key functions that have been proposed for eDNA include maintaining biofilm structural integrity, initiating adhesion to dental surfaces, acting as a nutrient source, and facilitating horizontal gene transfer. Thus, eDNA is a potential therapeutic target for the management of oral disease–associated biofilm. This review aims to summarize advances in the understanding of the mechanisms of eDNA release from oral microorganisms and in the methods of eDNA detection and quantification within oral biofilms.

scholarly journals Chemical Plaque Control Strategies in the Prevention of Biofilm-associated Oral Diseases

2016 ◽  
Vol 17 (4) ◽  
pp. 337-343 ◽  
Author(s):  
Sukumaran Anil ◽  
Shilpa H Bhandi ◽  
Elna P Chalisserry ◽  
Mohammed Jafer ◽  
Jagadish Hosmani
Keyword(s):  
Dental Caries ◽  
Dental Plaque ◽  
Control Strategies ◽  
Periodontal Diseases ◽  
Oral Diseases ◽  
Associated Diseases ◽  
Patient Cooperation ◽  
Control And Prevention ◽  
Plaque Control ◽  
Plaque Biofilm

ABSTRACT Dental plaque is a biofilm that forms naturally on the surfaces of exposed teeth and other areas of the oral cavity. It is the primary etiological factor for the most frequently occurring oral diseases, such as dental caries and periodontal diseases. Specific, nonspecific, and ecologic plaque hypothesis explains the causation of dental and associated diseases. Adequate control of biofilm accumulation on teeth has been the cornerstone of prevention of periodontitis and dental caries. Mechanical plaque control is the mainstay for prevention of oral diseases, but it requires patient cooperation and motivation; therefore, chemical plaque control agents act as useful adjuvants for achieving the desired results. Hence, it is imperative for the clinicians to update their knowledge in chemical antiplaque agents and other developments for the effective management of plaque biofilm-associated diseases. This article explores the critical analysis of various chemical plaque control strategies and the current trends in the control and prevention of dental plaque biofilm. How to cite this article Jafer M, Patil S, Hosmani J, Bhandi SH, Chalisserry EP, Anil S. Chemical Plaque Control Strategies in the Prevention of Biofilm-associated Oral Diseases. J Contemp Dent Pract 2016;17(4):337-343.

Understanding dental plaque; biofilm dynamics

1997 ◽  
Vol 14 (3) ◽  
pp. 91-94 ◽  
Author(s):  
Gregg A. DuPont
Keyword(s):  
Dental Plaque ◽  
Tooth Surfaces ◽  
Biofilm Structure ◽  
And Control ◽  
Plaque Biofilm

A biofilm is a well organized community of cooperating microorganisms. Biofilms predictably form in nature, in artificial environments, and on medically implanted prostheses and indwelling venous catheters. They also form on tooth surfaces in the form of dental plaque. The intent of this article is to aid in the understanding of dental plaque by exploring biofilm structure, nature and dynamics. Understanding plaque facilitates its treatment and control.

scholarly journals Virtual Screening and Biological Evaluation of Anti-Biofilm Agents Targeting LuxS in the Quorum Sensing System

2021 ◽  
Vol 16 (6) ◽  
pp. 1934578X2110196
Author(s):  
Zheng Liu ◽  
Lihua Zhang ◽  
Jincai Wang ◽  
Yanping Li ◽  
Yiqun Chang ◽  
...  
Keyword(s):  
Dental Caries ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Binding Mode ◽  
Biological Evaluation ◽  
Oral Diseases ◽  
Traditional Use ◽  
New Approach ◽  
Sensing System ◽  
Plaque Biofilm

Biofilm formation is considered as a crucial factor in various oral diseases, such as dental caries. The quorum sensing (QS) signaling system was proved to have a crucial role in the microbial dental plaque biofilm formation of Streptococcus mutans ( S. mutans). LuxS was critical to regulating the QS system and survival of the bacterium, and, therefore, compounds which target LuxS may be a potential therapy for dental caries. The binding activities of 37,170 natural compounds to LuxS were virtually screened in this study. Baicalein and paeonol were chosen for further research of the binding mode and ΔG values with LuxS. Both baicalein and paeonol inhibited the biofilm formation without influence on the growth of S. mutans. Baicalein also distinctly reduced the production of both rhamnolipids and acids. The results provide us with a new approach to combat dental caries instead of the traditional use of antibacterial chemicals.

Evaluation of Salivary Interleukin-1ß Levels and Oral Health in Pakistani Male Smokers

2021 ◽  
Vol 15 (6) ◽  
pp. 1158-1163
Author(s):  
S. A. Hamza ◽  
S. Asif ◽  
S. A. H. Bokhari
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Low Income ◽  
Smoking Status ◽  
Saliva Sample ◽  
Oral Disease ◽  
Oral Diseases ◽  
Missing Teeth ◽  
Significance Level ◽  
Inflammatory Biomarker

Aim: Smoking induces changes in salivary inflammatory biomarker levels associated with oral diseases. This study status and explored association among salivary Interleukin-1β, oral diseases and smoking. Methods: Data of male smokers of a private institute recruited for a randomized clinical trial is analyzed for this paper. Demographic and systemic information were collected. Oral disease status was examined and saliva sample collected for IL-1β levels. IL-1β levels and other study variables were analyzed with respect to smoking status categorized into smoking years and cigarettes per day. The student's t-test and one-way ANOVA were used for statistical analysis using SPSS version 22) with significance level of p≤0.050. Results: Analysis of baseline data of seventy-eight smokers showed elevated levels of IL-1β with increasing smoking, higher BMI. Smoking was higher among aged, married and low-income individuals. Pearson partial correlation analysis, after controlling age, marital status, education, income, and BMI, demonstrated a positive significant relationship of smoking per day with smoking years; dental caries with missing teeth and calculus; gingivitis with missing teeth and calculus; periodontitis with dental caries, calculus and gingivitis. Conclusion: This analysis demonstrates that levels of IL-1β were raised in smokers, however; there was no association with oral disease parameters. Large studies may be conducted to observe status and association of smoking, oral disease and salivary biomarkers. Keywords: Smoking, Oral Health, IL-1β, Males

Oral Health and Nutrition

2020 ◽  
Author(s):  
Carole. A. Palmer ◽  
Zhangmuge Cheng
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Periodontal Diseases ◽  
Oral Disease ◽  
Oral Diseases ◽  
Oral Infections ◽  
Surgeon General ◽  
Optimal Health ◽  
Health And Disease ◽  
Oral Conditions

Oral diseases are among the most prevalent diseases affecting global health. In his report on the crisis in oral disease in America, the Surgeon General warned that one cannot be truly healthy without oral health. Oral health means freedom from all oral health problems; tooth decay (dental caries), periodontal diseases, tooth loss, oral-facial pain, oral cancer and the effects of its treatment, oral infections, craniofacial birth defects and more. The relationships between oral conditions and systemic health and disease are many and synergistic, and most involve dietary and/or systemic nutritional factors. Diet and nutrition can play important roles in the etiology, prevention, and/or management of oral conditions, as they do in overall health and disease. Today, all health professionals and educators need to be aware of and consider oral issues and their possible diet/nutritional implications as a component of optimal health care and education. This review article provides a brief overview of how diet and nutrition impact and are impacted by oral conditions, and offers general guidelines and resources for providing meaningful interventions throughout the life cycle. This review contains 3 figures, 3 tables, and 57 references Key Words: biofilm, cariogenic, dental caries, dental plaque, ECC-early childhood caries, lactobacillus, mucositis, non-cariogenic, periodontal disease, Streptococcus mutans

scholarly journals Glaciodynamic context of subglacial bedform generation and preservation

1999 ◽  
Vol 28 ◽  
pp. 23-32 ◽  
Author(s):  
Chris D. Clark
Keyword(s):  
Ice Sheets ◽  
Ice Sheet ◽  
Shear Stresses ◽  
Morphometric Characteristics ◽  
Glacial Cycle ◽  
Sheet Flow ◽  
Ice Flow ◽  
Ice Stream ◽  
Highly Sensitive ◽  
Basal Shear

AbstractSubglacially-produced drift lineations provide spatially extensive evidence of ice flow that can be used to aid reconstructions of the evolution of former ice sheets. Such reconstructions, however, are highly sensitive to assumptions made about the glaciodynamic context of lineament generation; when during the glacial cycle and where within the ice sheet were they produced. A range of glaciodynamic contexts are explored which include: sheet-flow submarginally restricted; sheet-flow pervasive; sheet- flow patch; ice stream; and surge or re-advance. Examples of each are provided. The crux of deciphering the appropriate context is whether lineations were laid down time-trans-gressively or isochronously. It is proposed that spatial and morphometric characteristics of lineations, and their association with other landforms, can be used as objective criteria to help distinguish between these cases.A logically complete ice-sheet reconstruction must also account for the observed patches of older lineations and other relict surfaces and deposits that have survived erasure by subsequent ice flow. A range of potential preservation mechanisms are explored, including: cold- based ice; low basal-shear stresses; shallowing of the deforming layer; and basal uncoupling.

scholarly journals Study of Pseudomonas aeruginosa PPF-1 biofilm formation using microfluidics: effect of magnesium on biofilm detachment in engineered conduits

2021 ◽  
Author(s):  
William Y. Harvey ◽  
Cynthia Gagné-Thivierge ◽  
Sepideh Fakari ◽  
Jean Barbeau ◽  
Steve Charette ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Reference Strain ◽  
Mechanical Stability ◽  
Three Dimensional ◽  
Opportunistic Pathogen ◽  
Shear Stresses ◽  
Density Maps ◽  
Biofilm Detachment ◽  
Flow Systems ◽  
Dynamics Simulations

The bacterium Pseudomonas aeruginosa is an opportunistic pathogen in certain organisms, including humans, but can also survive and proliferate in natural and engineered water systems. Microfluidic technology can address hydrodynamic questions related to bacterial contamination of water flow systems and infrastructure. In this work, a microfluidic approach was devised to study the effect of shear stresses on biofilms from a dental unit waterline (DUWL)-isolated P. aeruginosa strain, PPF-1. During application of relevant shear stress levels to DUWLs, the response of the PPF-1 biofilm was observed and compared to a clinical P. aeruginosa reference strain, PAO1. The response measurements were repeated for biofilms exposed to additional Mg2+ ions. Using a microfluidic approach to transforming optical density maps into three-dimensional images, we applied computational fluid dynamics simulations and determined the critical shear stresses for biofilm sloughing. In the absence of Mg2+, PPF-1 biofilms showed weaker attachment than PAO1 biofilms, resulting in continuous slough/regrowth cycles triggered by applied shear stresses of 1.42 +/- 0.32 Pa. Introducing Mg2+ into the PPF-1 biofilm culture medium seemed to place the biofilm into a viscoplastic mechanical state, thereby increasing mechanical stability, which resulted in elevated tolerances to shear stresses up to a critical value of 5.43 +/- 1.52 Pa. This resulted in a propensity for less frequent but more catastrophic sloughing events like that observed for the PAO1 reference strain. This suggests that in a low ionic environment, biofilms from the PPF-1 strain can result in higher and more continuous ejection of biofilm materials, possibly leading to increased downstream colonization of engineered flow systems.

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