scholarly journals Oil Red O based method for Exosome Labelling and Detection

2022 ◽  
Author(s):  
Shikha Bharati ◽  
Km Anjaly ◽  
Shivani Thoidingjam ◽  
A B Tiku
Keyword(s):  
Micelle Formation ◽  
Cost Effective ◽  
Biomedical Sciences ◽  
Detection And Tracking ◽  
Diagnostic Potential ◽  
Real Time Tracking ◽  
Improved Methods ◽  
Oil Red O

With the realization of the role of exosomes in diseases especially cancer, exosome research is gaining popularity in biomedical sciences. To understand exosome biology, their labelling and tracking studies are important. New and improved methods of exosome labelling for detection and tracking of exosomes need to be developed to harness their therapeutic and diagnostic potential. In this paper, we report a novel, simple and effective method of labelling and detecting exosomes using Oil red O (ORO) which is a dye commonly used for lipid staining. Using ORO is a cost effective and easy approach with intense red colouration of stained exosomes. Further, the issues faced with commonly used lipophilic dyes for exosomes labelling such as long term persistence of dyes, aggregation and micelle formation of dyes, difficulty to distinguish dye particles from labelled exosomes and detection of large aggregates of dye or dye-exosome are not seen with ORO dye. This method shows good labelling efficiency of exosomes with very sensitive detection and real-time tracking of the cellular uptake of exosomes.

Building Evaluation Capital in Government: A Queensland Departmental Approach

2017 ◽  
Vol 17 (4) ◽  
pp. 39-49
Author(s):  
Sarah Goswami ◽  
Vicki Lane
Keyword(s):  
Low Cost ◽  
Cost Effective ◽  
Building Blocks ◽  
Decision Makers ◽  
Value For Money ◽  
Department Of Agriculture ◽  
Evaluation Capacity Building ◽  
Statutory Body

Increasingly, government departments are being held accountable for investment in public services. In Queensland the Financial Accountability Act 2009 (Queensland Treasury, 2016) requires that accountable officers and statutory bodies ‘achieve reasonable value for money by ensuring the operations of the department or statutory body are carried out efficiently, effectively and economically’ (Section 61). Whilst there is a directive for agencies to evaluate and demonstrate value for money, it has in practice been difficult to embed long term, as many systems and decision makers have neglected the role of organisation-wide evaluation capital. This paper will outline the work being undertaken in the Queensland Department of Agriculture and Fisheries (DAF) to implement an Impact and Investment Framework, which will support and embed evaluation in a multidisciplinary setting. A central tenant of this framework is ‘business empowerment and learning'—building the evaluation culture in the organisation by first establishing evaluation building blocks, through business empowerment, support and utility. The framework is comprised of five key elements and is built on the principles of evaluation and evaluation capacity building disciplines. It has been designed to be low-cost, effective and efficient, whilst enabling business improvement, meeting accountability needs and allowing the department to demonstrate the value of its work.

Role of an Effective Hospital Information System in a Depressed Economy

1997 ◽  
Vol 36 (02) ◽  
pp. 141-143
Author(s):  
J. A. Olabode ◽  
A. A. Osunlaja
Keyword(s):  
Health Care ◽  
Information System ◽  
Hospital Information System ◽  
Care Delivery ◽  
Cost Effective ◽  
Practical Experience ◽  
University Hospital ◽  
Economic Use

Abstract:This work is based on practical experience acquired in the development of a hospital information system in a university hospital in Nigeria. The paper discusses how accurate, adequate and timely medical data can promote economy in health-care delivery in a depressed economy. Existing constraints are identified and solutions offered for an effective hospital information system. An effort has been made to illustrate how the application of informatics technology can be cost effective in the long-term, by ensuring effective and economic use of facilities and resources. Once an effective hospital information is operative, provision of affordable health care in a depressed economy would be feasible. To this end, the need for cooperation between countries to support technology and manpower is emphasized.

scholarly journals THE ROLE OF YOGA THERAPY IN THE MANAGEMENT OF BRONCHIAL ASTHMA (TAMAKA SHWASA)

2019 ◽  
pp. 27-33
Author(s):  
ARPAN A BHATT ◽  
SHWETA PANDEY ◽  
BRAJESH SINGH ◽  
GAURAVI VYAS ◽  
GYANENDRA DATTA SHUKLA ◽  
...  
Keyword(s):  
Bronchial Asthma ◽  
Cost Effective ◽  
Large Sample Size ◽  
Oxyhemoglobin Saturation ◽  
Yoga Therapy ◽  
Beneficial Effects ◽  
Strengthening Exercises ◽  
Airway Exchange

Objective: The present study was conducted on patients taken from Swasthavrutta department of Gujarat Ayurved University in Jamnagar town, Gujarat. The study was done with an objective of providing a safe and reliable as well as cost-effective natural management of symptoms occurring in patients of bronchial asthma. This is also done to reduce the various harmful effects of modern medicines among the patients and to reduce the use and dependency on inhalers. Methods: Thirty-two patients with bronchial asthma (Tamaka Shwasa) underwent training for 16 weeks in an integrated set of yoga exercises, including prayer, strengthening exercises, Yogasanas (including standing Asana, prone Asana, sitting Asana, and supine Asana), Pranayama (Nadi Shodhana, Ujjayi, Bhramari, and Aumkara Dharana), and Shuddhi Kriya (to be done once in a week) which includes Kunjal, Sutraneti, and Kapalabhati. The patients were told to practice these exercises for 60 min daily. Results: Significant improvement was seen in the patients who undergone yogic interventions. There was significant improvement in the duration of Shwasakashtata, frequency of Shwasakashtata, reduction in taking number of emergency medicine, Pinasa, Kaphashtheevana, cough, Ghurghuraka, orthopnea, Parshvashula, Lalate Sweda, result on peak expiratory flow rate, airway exchange catheters, oxyhemoglobin saturation, and chest expansion. Conclusion: From the study, it can be concluded that yoga practices can be opted as long-term management of bronchial asthma, but its beneficial effects need to be studied in more details on large sample size.

scholarly journals Ayurveda and Modern Perspective on Rajonivrittiw.s.r. to Menopause: Review Based on Literary Study

2021 ◽  
Vol 04 (11) ◽  
pp. 109-113
Author(s):  
Monika Kumari ◽  
Mahesh Dixit ◽  
Narendra Kumar Meena
Keyword(s):  
Hormone Replacement ◽  
Cost Effective ◽  
Modern Medicine ◽  
The Body ◽  
Term Therapy ◽  
Cost Effective Treatment ◽  
Long Term Therapy ◽  
Modern Perspective

Menopause, a phenomenon unique to women, is the ending of a woman’s monthly menstrual period and ovulation. It, alongside, several other changes in the bodyand mind, brought in due to a decline in the amount of the hormones estrogen and progesterone being produced by the body. Menopause is, occasionally, viewed as an end to youth and sexuality, making it a socially unacceptable occurrence. Menopause, though,one of the important physical and mental milestones in a woman’s life, many women lack information about what is taking place and what are their options. There comes the role of Ayurveda , in Ayurveda literature there is no detailed description of Rajonivrittior menopause except the age of Rajonivrittiis given as 50 years. Ayurveda involves a holistic physiological system based on balance, with its cardinal doctrine of human physiology being constituted by “vata”, “pitta” and “kapha”. In modern medicine the only treatment for these symptoms is Hormone replacement therapy (HRT), which is not a long-term therapy to be given as it has side effects on the overall health of the women. In Ayurveda Dhatu kshaya can be treated with Rasayanatherapy and other symptoms of hypo-estrogenic conditions can be treated with Phytoestrogens. So, Ayurveda gives a non-hormonal and cost-effective treatment for Rajonivrittior Menopause.

Physical and Psychological Benefits of Written Emotional Expression

2015 ◽  
Vol 20 (4) ◽  
pp. 242-251 ◽  
Author(s):  
Éva Kállay
Keyword(s):  
Mental Health ◽  
Expressive Writing ◽  
Mental Health Problems ◽  
Cost Effective ◽  
Health Problems ◽  
Future Research ◽  
Psychological Benefits ◽  
Cost Effective Treatment ◽  
Meta Analyses

Abstract. The last several decades have witnessed a substantial increase in the number of individuals suffering from both diagnosable and subsyndromal mental health problems. Consequently, the development of cost-effective treatment methods, accessible to large populations suffering from different forms of mental health problems, became imperative. A very promising intervention is the method of expressive writing (EW), which may be used in both clinically diagnosable cases and subthreshold symptomatology. This method, in which people express their feelings and thoughts related to stressful situations in writing, has been found to improve participants’ long-term psychological, physiological, behavioral, and social functioning. Based on a thorough analysis and synthesis of the published literature (also including most recent meta-analyses), the present paper presents the expressive writing method, its short- and long-term, intra-and interpersonal effects, different situations and conditions in which it has been proven to be effective, the most important mechanisms implied in the process of recovery, advantages, disadvantages, and possible pitfalls of the method, as well as variants of the original technique and future research directions.

Abusive head trauma (AHT) outcome at long-term follow-up and the role of elective neurosurgical approach: A report of 22 cases

2013 ◽  
Author(s):  
Francesca Menegazzo ◽  
Melissa Rosa Rizzotto ◽  
Martina Bua ◽  
Luisa Pinello ◽  
Elisabetta Tono ◽  
...  
Keyword(s):  
Head Trauma ◽  
Abusive Head Trauma ◽  
Long Term Follow Up

Antithrombotic Therapy for DVT/PE

1997 ◽  
Vol 17 (03) ◽  
pp. 161-162
Author(s):  
Thomas Hyers
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Oral Anticoagulants ◽  
Cost Effective ◽  
Therapeutic Agents ◽  
Great Promise ◽  
Term Therapy ◽  
Low Molecular Weight ◽  
Long Term Therapy

SummaryProblems with unfractionated heparin as an antithrombotic have led to the development of new therapeutic agents. Of these, low molecular weight heparin shows great promise and has led to out-patient therapy of DVT/PE in selected patients. Oral anticoagulants remain the choice for long-term therapy. More cost-effective ways to give oral anticoagulants are needed.

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