scholarly journals Expression profile analysis of circular RNAs in essential hypertension by microarray and bioinformatics

2018 ◽  
Author(s):  
Xingjie-Bao ◽  
Xin-He ◽  
Shuying-Zheng ◽  
Yizhe-Luo ◽  
Tianlun-Gu ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Expression Profile ◽  
Microarray Data ◽  
Profile Analysis ◽  
Circular Rnas ◽  
Healthy Controls ◽  
Biological Functions ◽  
Qrt Pcr ◽  
Expression Profile Analysis ◽  
Public Data

AbstractCircular RNAs (circRNAs), widely found in human cells, are involved in disease and play an important role in progression. To determine whether circRNAs are related in essential hypertension (EH), we analyzed the expression profile of circRNAs and miRNAs in 5 EH and 5 healthy controls cases which were screened by microarray. Through microarray data and public data analysis, differently expressed transcripts were divided into modules, and circRNAs were functionally annotated by miRNAs. The expression of two circRNAs, has_circ_0037909 and has_circ_0105015, were validated in EH by qRT-PCR, which may be associated with EH. Further analysis showed that two circRNAs might through immune system by up-regulation circRNAs and down-regulation expression. These circRNAs biological functions need to be further validated.

scholarly journals Microarray Expression Profile of Circular RNAs in Plasma from Primary Biliary Cholangitis Patients

2017 ◽  
Vol 44 (4) ◽  
pp. 1271-1281 ◽  
Author(s):  
Jiajia Zheng ◽  
Zhenrong Li ◽  
Tiancheng Wang ◽  
Yang Zhao ◽  
Yongfeng Wang
Keyword(s):  
Expression Profile ◽  
Expression Profiles ◽  
Characteristic Curve ◽  
Group Versus ◽  
Target Prediction ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Primary Biliary Cholangitis ◽  
Healthy Controls ◽  
Qrt Pcr

Background/Aims: Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases, including autoimmune diseases. However, their role in primary biliary cholangitis (PBC) remains unclear. Here, we aimed to determine the circRNA expression profile in plasma from PBC patients and further explore the value of circRNA in diagnosing PBC. Methods: CircRNA microarrays were used to determine circRNA expression profiles in plasma samples from 6 PBC patients and 6 healthy controls. Statistical analyses identified differentially expressed circRNAs, and these circRNAs were verified by qRT-PCR in 29 PBC patients and 30 healthy controls. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a map of circRNA-miRNA interactions for the differentially expressed circRNAs. Results: Our results indicated that there were 18 up-regulated and 4 down-regulated circular RNAs in the plasma from PBC patients compared with that from healthy individuals. Among the differentially expressed circRNAs, hsa_circ_402458 (P=0.0033), hsa_circ_087631 and hsa_circ_406329 (P=0.0185) were up-regulated, and hsa_circ_407176 (P=0.0066) and hsa_circ_082319 were down-regulated in the PBC group versus the healthy group as demonstrated by qRT-PCR. In particular, hsa_circ_402458 was significantly higher in PBC patients not receiving UDCA treatment than in PBC patients receiving UDCA treatment (P=0.0338). The area under the receiver operating characteristic curve for hsa_circ_402458 for diagnosing PBC was 0.710 (P=0.005). For hsa_circ_402458, two putative miRNA targets, hsa-miR-522-3p and hsa-miR-943, were predicted. Conclusions: circRNA dysregulation may play a role in PBC pathogenesis, and hsa_circ_402458 shows promise as a candidate biomarker for PBC.

scholarly journals Expression Profile Analysis of Differentially Expressed Circular RNAs in Steroid-Induced Osteonecrosis of the Femoral Head

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Zhongxin Zhu ◽  
Wenxi Du ◽  
Huan Yu ◽  
Hongting Jin ◽  
Peijian Tong
Keyword(s):  
Femoral Head ◽  
Bioinformatics Analysis ◽  
Profile Analysis ◽  
Circular Rnas ◽  
Healthy Individuals ◽  
Bioinformatics Analyses ◽  
Diagnostic Biomarkers ◽  
Qrt Pcr ◽  
Expression Profile Analysis

Background. A growing number of studies have suggested that circular RNAs (circRNAs) serve as potential diagnostic biomarkers in many diseases. However, the role of circRNAs in steroid-induced osteonecrosis of the femoral head (SONFH) has not been reported. Methods. Secondary sequencing was performed to profile circRNA expression in peripheral blood samples from three SONFH patients and three healthy individuals. We confirmed our preliminary findings by qRT-PCR. Bioinformatics analysis was conducted to predict their functions. Results. The result showed 345 dysregulated circRNAs. qRT-PCR of eight selected circRNAs preliminarily confirmed the results, which were consistent with RNA sequencing. Bioinformatics analyses were performed to predict the functions of circRNAs to target the genes of miRNAs and the networks of circRNA-miRNA-mRNA interactions. Conclusions. This study provides a new and fundamental circRNA profile of SONFH and a theoretical basis for further studies on the functions of circRNAs in SONFH.

scholarly journals Expression Profile Analysis of Circular RNAs in Ovarian Endometriosis by Microarray and Bioinformatics

2018 ◽  
Vol 24 ◽  
pp. 9240-9250 ◽  
Author(s):  
Mengmeng Zhang ◽  
Chutong Ren ◽  
Yinan Xiao ◽  
Xiaomeng Xia ◽  
Xiaoling Fang
Keyword(s):  
Expression Profile ◽  
Profile Analysis ◽  
Circular Rnas ◽  
Ovarian Endometriosis ◽  
Expression Profile Analysis

Expression profile analysis of circular RNAs in BmN cells ( Bombyx mori ) upon BmNPV infection

2020 ◽  
Vol 105 (3) ◽  
Author(s):  
Shaolun Zhang ◽  
Manman Shen ◽  
Haotong Yin ◽  
Haoling Huang ◽  
Tao Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Expression Profile ◽  
Profile Analysis ◽  
Circular Rnas ◽  
Expression Profile Analysis ◽  
Bmn Cells

scholarly journals Gene expression profile analysis of pancreatic cancer based on microarray data

2016 ◽  
Vol 13 (5) ◽  
pp. 3913-3919 ◽  
Author(s):  
JIN LONG ◽  
ZHE LIU ◽  
XINGDA WU ◽  
YUANHONG XU ◽  
CHUNLIN GE
Keyword(s):  
Gene Expression ◽  
Pancreatic Cancer ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Microarray Data ◽  
Profile Analysis ◽  
Expression Profile Analysis

scholarly journals CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients

2017 ◽  
Vol 44 (6) ◽  
pp. 2138-2146 ◽  
Author(s):  
Jiangmin Zhao ◽  
Le Li ◽  
Qian Wang ◽  
Hongxiu Han ◽  
Qing Zhan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Early Diagnosis ◽  
Lung Adenocarcinoma ◽  
Expression Profile ◽  
Microarray Data ◽  
Mammalian Cells ◽  
Early Stage ◽  
Circular Rnas ◽  
Cancer Disease ◽  
Qrt Pcr

Background/Aims: Lung adenocarcinoma, a form of non-small cell lung cancer with high lethality at an advanced stage, is becoming more common in women, non- or never-smokers, and even young adults. At present, there are still no effective early diagnosis methods for patients to be cured in a timely manner. Circular RNAs (circRNAs), which are stable and conserved non-coding RNA in mammalian cells, have been reported to be widely involved in the processes of cancer disease. However, it is still a puzzle as to which specific circRNAs are involved in the development of early-stage lung adenocarcinoma. Methods: Tumor samples and paired adjacent normal tissues from 4 patients with early-stage lung adenocarcinoma were selected to investigate the expression profile of circRNAs by using a high-throughput circRNA microarray. Bioinformatic analyses were conducted to screen those differentially expressed circRNAs. qRT-PCR and sequencing were performed to assure the microarray data. Results: A total of 357 circRNAs were dysregulated in the tumor samples, which suggests potential roles in lung cancer. qRT-PCR detection showed that five selected circRNAs were identical to the microarray data, and the potential circRNA-miRNA interactions were predicted. Conclusion: This work illustrates that clusters of circRNAs are aberrantly expressed in early-stage lung adenocarcinoma, which might offer potential targets for the early diagnosis of this disease and new genetic insights into lung cancer.

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