Antibiotic resistance is lower in Staphylococcus aureus isolated from antibiotic-free raw meat as compared to conventional raw meat

AbstractThe frequent use of antibiotics contributes to antibiotic resistance in bacteria, resulting in an increase in infections that are difficult to treat. Livestock are commonly administered antibiotics in their feed, but there is current interest in raising animals that are only administered antibiotics during active infections. Staphylococcus aureus (SA) is a common pathogen of both humans and livestock raised for human consumption. SA has achieved high levels of antibiotic resistance, but the origins and locations of resistance selection are poorly understood. We determined the prevalence of SA and MRSA in conventional and antibiotic-free (AF) meat products, and also measured rates of antibiotic resistance in these isolates. We isolated SA from raw conventional turkey, chicken, beef, and pork samples and also from AF chicken and turkey samples. We found that SA contamination was common, with an overall prevalence of 22.64% (range of 2.78-30.77%) in conventional meats and 13.0% (range of 12.5-13.2%) in AF poultry meats. MRSA was isolated from 15.72% of conventional raw meats (range of 2.78-20.41%) but not from AF-free meats. The degree of antibiotic resistance in conventional poultry products was significantly higher vs AF poultry products for a number of different antibiotics, and while multi-drug resistant strains were relatively common in conventional meats none were detected in AF meats. The use of antibiotics in livestock contributes to high levels of antibiotic resistance in SA found in meat products. Our results support the use of AF conditions for livestock in order to prevent antibiotic resistance development in SA.

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Evidence of Another Anthropic Impact on Iguana delicatissima from the Lesser Antilles: The Presence of Antibiotic Resistant Enterobacteria

Antibiotics ◽

10.3390/antibiotics10080885 ◽

2021 ◽

Vol 10 (8) ◽

pp. 885

Author(s):

Gustavo Di Lallo ◽

Marco Maria D’Andrea ◽

Samanta Sennati ◽

Maria Cristina Thaller ◽

Luciana Migliore ◽

...

Keyword(s):

Antibiotic Resistance ◽

Animal Species ◽

Susceptibility Testing ◽

Acquired Resistance ◽

Climatic Conditions ◽

Antibiotic Resistant ◽

Associated Bacteria ◽

Improper Use ◽

Resistant Strains ◽

Drug Resistant Strains

The improper use of antibiotics by humans may promote the dissemination of resistance in wildlife. The persistence and spread of acquired antibiotic resistance and human-associated bacteria in the environment, while representing a threat to wildlife, can also be exploited as a tool to monitor the extent of human impact, particularly on endangered animal species. Hence, we investigated both the associated enterobacterial species and the presence of acquired resistance traits in the cloacal microbiota of the critically endangered lesser Antillean iguana (Iguana delicatissima), by comparing two separate populations living in similar climatic conditions but exposed to different anthropic pressures. A combination of techniques, including direct plating, DNA sequencing and antimicrobial susceptibility testing allowed us to characterize the dominant enterobacterial populations, the antibiotic resistant strains and their profiles. A higher frequency of Escherichia coli was found in the samples from the more anthropized site, where multi-drug resistant strains were also isolated. These results confirm how human-associated bacteria as well as their antibiotic-resistance determinants may be transferred to wildlife, which, in turn, may act as a reservoir of antibiotic resistance.

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Enterotoxigenic and Antibiotic Resistance Determination of Staphylococcus aureus Strains Isolated from Food Handlers in Gaborone, Botswana

Journal of Food Protection ◽

10.4315/0362-028x-70.12.2764 ◽

2007 ◽

Vol 70 (12) ◽

pp. 2764-2768 ◽

Cited By ~ 22

Author(s):

DANIEL LOETO ◽

M. I. MATSHEKA ◽

B. A. GASHE

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Nasal Cavity ◽

Penicillin G ◽

Food Handlers ◽

Carriage Rate ◽

Methicillin Resistant ◽

Resistant Strains ◽

High Standards

The prevalence, antibiotic resistance, and enterotoxigenic potential of Staphylococcus aureus strains from different anatomical sites on food handlers in Gaborone, Botswana, were determined. Of a total of 200 food handlers tested, 115 (57.5%) were positive for S. aureus. Of the 204 S. aureus isolates, 63 (30.9%), 91 (44.6%), and 50 (24.5%) were isolated from the hand, nasal cavity, and face, respectively, and 43 (21%) of the isolates were enterotoxigenic. The most prevalent enterotoxin was type A, which accounted for 34.9% of all the enterotoxigenic strains, and enterotoxin D was produced by the fewest number of strains (9.3%). Resistance to methicillin was encountered in 33 (22.4%) of the penicillin G–resistant isolates, and 9 (27.3%) of these methicillin-resistant isolates also were resistant to vancomycin. Nineteen antibiotic resistance profiles were determined, and the nasal cavity had the highest diversity of resistance profiles. The nasal cavity also had the highest number of resistant strains, 77 (53%), whereas the hand and face had 49 (32%) and 24 (16.0%) resistant strains, respectively. To reduce the Staphylococcus carriage rate among food handlers, training coupled with a commitment to high standards of personal and environmental hygiene is recommended.

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Antibiotic Resistance and Virulence Gene Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Healthy Edible Marine Fish

International Journal of Microbiology ◽

10.1155/2020/9803903 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Justine Fri ◽

Henry A. Njom ◽

Collins N. Ateba ◽

Roland N. Ndip

Keyword(s):

Staphylococcus Aureus ◽

Antibiotic Resistance ◽

Marine Fish ◽

Control Strategies ◽

Virulence Gene ◽

Multidrug Resistant ◽

Methicillin Resistant ◽

Vancomycin Mics ◽

Resistant Strains ◽

Mrsa Strains

Thirty-three (33) isolates of methicillin-resistant Staphylococcus aureus (MRSA) from healthy edible marine fish harvested from two aquaculture settings and the Kariega estuary, South Africa, were characterised in this study. The phenotypic antimicrobial susceptibility profiles to 13 antibiotics were determined, and their antibiotic resistance determinants were assessed. A multiplex PCR was used to determine the epidemiological groups based on the type of SCCmec carriage followed by the detection of staphylococcal enterotoxin-encoding genes sea-sed and the Panton Valentine leucocidin gene (pvl). A high antibiotic resistance percentage (67–81%) was observed for Erythromycin, Ampicillin, Rifampicin, and Clindamycin, while maximum susceptibility to Chloramphenicol (100%), Imipenem (100%), and Ciprofloxacin (94%) was recorded. Nineteen (58%) of the MRSA strains had Vancomycin MICs of ≤2 μg/mL, 4 (12%) with MICs ranging from 4–8 μg/mL, and 10 (30%) with values ≥16 μg/mL. Overall, 27 (82%) isolates were multidrug-resistant (MDR) with Erythromycin-Ampicillin-Rifampicin-Clindamycin (E-AMP-RIP-CD) found to be the dominant antibiotic-resistance phenotype observed in 4 isolates. Resistance genes such as tetM, tetA, ermB, blaZ, and femA were detected in two or more resistant strains. A total of 19 (58%) MRSA strains possessed SCCmec types I, II, or III elements, characteristic of healthcare-associated MRSA (HA-MRSA), while 10 (30%) isolates displayed SCCmec type IVc, characteristic of community-associated MRSA (CA-MRSA). Six (18%) of the multidrug-resistant strains of MRSA were enterotoxigenic, harbouring the see, sea, or sec genes. A prevalence of 18% (6/33) was also recorded for the luk-PVL gene. The findings of this study showed that marine fish contained MDR-MRSA strains that harbour SCCmec types, characteristic of either HA-MRSA or CA-MRSA, but with a low prevalence of enterotoxin and pvl genes. Thus, there is a need for continuous monitoring and implementation of better control strategies within the food chain to minimise contamination of fish with MDR-MRSA and the ultimate spread of the bug.

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In Vitro Activity of Gepotidacin (GSK2140944) against Neisseria gonorrhoeae

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02047-16 ◽

2017 ◽

Vol 61 (3) ◽

Cited By ~ 22

Author(s):

D. J. Farrell ◽

H. S. Sader ◽

P. R. Rhomberg ◽

N. E. Scangarella-Oman ◽

R. K. Flamm

Keyword(s):

Neisseria Gonorrhoeae ◽

Single Step ◽

Topoisomerase Iv ◽

Bacterial Dna ◽

Content Type ◽

Resistance Selection ◽

Resistant Strains ◽

Drug Resistant Strains ◽

Time Kill

ABSTRACT Gepotidacin (formerly GSK2140944) is a novel, first-in-class, triazaacenaphthylene antibacterial that inhibits bacterial DNA gyrase and topoisomerase IV via a unique mechanism and has demonstrated in vitro activity against Neisseria gonorrhoeae, including drug-resistant strains, and also targets pathogens associated with other conventional and biothreat infections. Broth microdilution was used to evaluate the MIC and minimum bactericidal concentration (MBC) activity of gepotidacin and comparators against 25 N. gonorrhoeae strains (including five ciprofloxacin-nonsusceptible strains). Gepotidacin activity was also evaluated against three N. gonorrhoeae strains (including a ciprofloxacin-nonsusceptible strain) for resistance development, against three N. gonorrhoeae strains (including two tetracycline- and azithromycin-nonsusceptible strains) using time-kill kinetics and checkerboard methods, and against two N. gonorrhoeae strains for the investigation of postantibiotic (PAE) and subinhibitory (PAE-SME) effects. The MIC50 and MIC90 for gepotidacin against the 25 N. gonorrhoeae isolates tested were 0.12 and 0.25 μg/ml, respectively. The MBC50 and MBC90 for gepotidacin were 0.25 and 0.5 μg/ml, respectively. Gepotidacin was bactericidal, and single-step resistance selection studies did not recover any mutants, indicating a low rate of spontaneous single-step resistance. For combinations of gepotidacin and comparators tested using checkerboard methods, there were no instances where antagonism occurred and only one instance of synergy (with moxifloxacin; fractional inhibitory concentration, 0.375). This was not confirmed by in vitro time-kill studies. The PAE for gepotidacin against the wild-type strain ranged from 0.5 to >2.5 h, and the PAE-SME was >2.5 h. These in vitro data indicate that further study of gepotidacin is warranted for potential use in treating infections caused by N. gonorrhoeae.

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Impact of Cooking, Cooling, and Subsequent Refrigeration on the Growth or Survival of Clostridium perfringens in Cooked Meat and Poultry Products

Journal of Food Protection ◽

10.4315/0362-028x-66.7.1227 ◽

2003 ◽

Vol 66 (7) ◽

pp. 1227-1232 ◽

Cited By ~ 39

Author(s):

ROBIN M. KALINOWSKI ◽

R. BRUCE TOMPKIN ◽

PETER W. BODNARUK ◽

W. PAYTON PRUETT

Keyword(s):

Public Health ◽

Food Safety ◽

Health Risk ◽

Clostridium Perfringens ◽

Meat Products ◽

Performance Standards ◽

Public Health Risk ◽

Raw Meat ◽

Poultry Products ◽

Cooked Meat

In January 1999, the Food Safety and Inspection Service (FSIS) finalized performance standards for the cooking and chilling of meat and poultry products in federally inspected establishments. More restrictive chilling (stabilization)requirements were adopted despite the lack of strong evidence of a public health risk posed by industry practices employing the original May 1988 guidelines (U.S. Department of Agriculture FSIS Directive 7110.3). Baseline data led the FSIS to estimate a “worst case” of 104 Clostridium perfringens cells per g in raw meat products. The rationale for the FSIS performance standards was based on this estimate and the assumption that the numbers detected in the baseline study were spores that could survive cooking. The assumptions underlying the regulation stimulated work in our laboratory to help address why there have been so few documented outbreaks of C. perfringens illness associated with the consumption of commercially processed cooked meat and poultry products. Our research took into account the numbers of C. perfringens spores in both raw and cooked products. One hundred ninety-seven raw comminuted meat samples were cooked to 73.9°C and analyzed for C. perfringens levels. All but two samples had undetectable levels (<3 spores per g). Two ground pork samples contained 3.3 and 66 spores per g. Research was also conducted to determine the effect of chilling on the outgrowth of C. perfringens spores in cured and uncured turkey. Raw meat blends inoculated with C. perfringens spores, cooked to 73.9°C, and chilled according to current guidelines or under abuse conditions yielded increases of 2.25 and 2.44 log10 CFU/g for uncured turkey chilled for 6 h and an increase of 3.07 log10 CFU/g for cured turkey chilled for 24 h. No growth occurred in cured turkey during a 6-h cooling period. Furthermore, the fate of C. perfringens in cooked cured and uncured turkey held at refrigeration temperatures was investigated. C. perfringens levels decreased by 2.52, 2.54, and 2.75 log10 CFU/g in cured turkey held at 0.6, 4.4, and 10°C, respectively, for 7 days. Finally, 48 production lots of ready-to-eat meat products that had deviated from FSIS guidelines were analyzed for C. perfringens levels. To date, 456 samples have been tested, and all but 25 (ranging from 100 to 710 CFU/g) of the samples contained C. perfringens at levels of <100 CFU/g. These results further support historical food safety data that suggest a very low public health risk associated with C. perfringens in commercially processed ready-to-eat meat and poultry products.

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Microbial quality of herbal juices sold at sport complex in Washim

International Journal of Research in Medical Sciences ◽

10.18203/2320-6012.ijrms20174950 ◽

2017 ◽

Vol 5 (11) ◽

pp. 4946 ◽

Cited By ~ 1

Author(s):

Nikhilesh Kulkarni ◽

Jayshree Narwade ◽

Rachana Pachori ◽

Prithviraj Sadar

Keyword(s):

Age Groups ◽

Human Consumption ◽

Multiple Drug ◽

Microbial Quality ◽

The People ◽

Multiple Drug Resistant ◽

Resistant Strains ◽

Human Infections ◽

Drug Resistant Strains

Background: Herbal juices are gaining global attention due to their medicinal properties, nutritive and antioxidant activity. They are frequently consumed by the people of all age groups in the form of health drinks. However, many outbreaks of human infections have been reported to be associated with the consumption of contaminated herbal juices. Hence, microbial quality assessment of herbal juices is utmost important.Methods: The present study was undertaken to detect the existence of enteric pathogens as well as coliforms in the herbal juices sold at sport complex in Washim city area. The antimicrobial susceptibility testing was also performed to evaluate the MDR status of the isolates.Results: The results showed that most of the herbal juices are contaminated with Salmonella, Shigella and Coliforms. The pathogens were found to be multiple drug resistant strains which pose an alarming threat for the consumers.Conclusions: Regular monitoring of the quality of herbal juices for human consumption is recommended to avoid disease outbreak.

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PREVALENCE OF ANTIBIOTIC RESISTANT STAPHYLOCOCCUS AUREUS IN RAW MEAT SAMPLES INTENDED FOR HUMAN CONSUMPTION

International Journal of Biology Pharmacy and Allied Sciences ◽

10.31032/ijbpas/2020/9.3.4938 ◽

2020 ◽

Vol 9 (3) ◽

Keyword(s):

Staphylococcus Aureus ◽

Human Consumption ◽

Antibiotic Resistant ◽

Raw Meat ◽

Meat Samples

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Molecular detection of Staphylococcal enterotoxins and mecA genes products in food samples collected from different areas in Khartoum state

10.21203/rs.3.rs-59354/v1 ◽

2020 ◽

Author(s):

Mohammed Yahya ◽

Hashim Abdalbagi Ali ◽

Babbiker Mohammed Taher Gorish ◽

Sara Omer Ali ◽

Eman Saif Aldein Abdalrhim ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Meca Gene ◽

Food Poisoning ◽

Meat Products ◽

Low Frequency ◽

Raw Milk ◽

Food Samples ◽

Enterotoxin Genes ◽

Resistant Strains ◽

Enterotoxin B

Abstract Background Staphylococcal Food Poisoning is an intoxication that results from the consumption of improperly prepared or stored foods containing sufficient amounts of one or more preformed S. aureus enterotoxins. Now days many researchers worldwide noted an emerging of resistant strains Staphylococci especially for the antibiotic Methicillin. Therefore, this study was aimed to determine the existence of Staphylococcus aureus and its enterotoxins, mecA genes in food samples. Results A total of 400 samples were collected from different areas in Khartoum state. The type of foods included Cheese, Meat products, Fish and Raw milk, 100 samples for each. out of 400 samples cultivated 137 (34.25%) isolates were identified as S. aureus, 126 (31.5%) were identified as bacteria other than S. aureus and 137 (34.25%) were yield no growth. Of 137 S.aureus isolates, 84 were randomly selected and examined for the presence mecA and enterotoxin genes products. Oxacillin sensitivity test showed that 15(11%) of 137 S.aureus isolates were Oxacillin resistant. The PCR assay showed that mecA gene was detected in 15 of 84 (17%) S. aureus isolates. While only 2 (2.385%) out of 84 S. aureus isolates were show an enterotoxin B gene product. Conclusion There was a relatively moderate prevalence of Methicillin resistant staphylococcus aureus with very low frequency of enterotoxin B gene in different kinds of food samples which collected from Khartoum state. These findings highlight the high potential risk for consumers of meat and dairy products especially in the absence of strict hygienic and preventive measures to avoid Staphylococcus aureus enterotoxins production in foods.

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Effects of Tormentic Acid and the Extracts from Callistemon citrinus on the Production of Extracellular Proteases by Staphylococcus aureus

Biochemistry Research International ◽

10.1155/2020/6926320 ◽

2020 ◽

Vol 2020 ◽

pp. 1-6

Author(s):

Rumbidzai Mashezha ◽

Molly Mombeshora ◽

Stanley Mukanganyama

Keyword(s):

Staphylococcus Aureus ◽

Ethanol Extract ◽

Extracellular Protease ◽

Protease Production ◽

Extracellular Proteases ◽

Therapeutic Molecules ◽

Antibacterial Susceptibility ◽

Resistant Strains ◽

Drug Resistant Strains ◽

Tormentic Acid

Staphylococcus aureus is among the common nosocomial pathogens. Antibiotics have been used to treat S. aureus infections. However, there has been increased mortality associated with drug-resistant strains of S. aureus. Extracellular proteases have been implicated to be responsible for the transition of S. aureus from an adhesive pathogen to an invasive pathogen. The development of resistant strains has necessitated the search for new sources of drugs. Plants have been traditionally used as sources of therapeutic molecules. The objective of this study was to determine the effect of tormentic acid and the extracts from Callistemon citrinus on the production of extracellular proteases by S. aureus. The broth microdilution antibacterial susceptibility assay was used to determine the antibacterial effects of tormentic acid and the extracts on S. aureus. Both extracts showed a minimum inhibitory concentration (MIC) value of 50 μg/ml. The water : ethanol (50 : 50) and the dichloromethane : methanol (50 : 50) extracts were found to be bactericidal against S. aureus at a concentration of 100 μg/ml and 50 μg/ml, respectively. The effect of tormentic acid and extracts on extracellular protease production was investigated using the protease assay. A zone of proteolytic activity (Pr) was measured as the ratio of the diameter of the colony to the total diameter of colony plus zone of hydrolysis. The extracts reduced the production of extracellular proteases, while tormentic acid completely inhibited the production of extracellular proteases by S. aureus. The Pr value for tormentic acid was found to be 1. The Pr values of the dichloromethane : methanol extract and the water : ethanol extract were 0.92 and 0.84, respectively. In conclusion, tormentic acid was shown to inhibit extracellular protease production; therefore, there is need to explore its use in antivirulence therapy to combat S. aureus infections.

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