scholarly journals Manure substitution of chemical fertilizer affect soil microbial community diversity, structure and function in greenhouse vegetable production systems

2019 ◽  
Author(s):  
Haoan Luan ◽  
Wei Gao ◽  
Shaowen Huang ◽  
Jiwei Tang ◽  
Mingyue Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Physicochemical Properties ◽  
Soil Microbial Community ◽  
Enzyme Activities ◽  
Substitution Rate ◽  
Soil Microbial Communities ◽  
Chemical Fertilizer ◽  
Soil Physicochemical Properties ◽  
Soil Microbial ◽  
Microbial Characteristics

ABSTRACTSoil microbial community and enzyme activities together affect various ecosystem functions of soils. Fertilization, as important agricultural management practices, are known to modify soil microbial characteristics; however, inconsistent results have been reported. The aim of this research therefore was to make a comparative study of the effects of different fertilization patterns (No N inputs (No N), 100% chemical fertilizer-N (CN) inputs (4/4CN) and different substitution rates of CN by organic manure-N (MN) (3/4CN+1/4MN, 2/4CN+2/4MN and 1/4CN+3/4MN)) on soil physicochemical properties, enzyme activities and microbial attributes in a GVP of Tianjin, China. Manure substitution of chemical fertilizer, especially at higher substitution rate (2/4CN+2/4MN and 1/4CN+3/4MN), improved soil physicochemical properties (higher soil organic C (SOC) and nutrient contents; lower bulk densities), promoted microbial growth (higher total phospholipid fatty acids and microbial biomass C contents) and activity (higher soil hydrolase activities). Manure addition caused a remarkable increase of the fungi/bacteria ratio and a distinct shift in the fungal (bacterial) community to greater abundance of arbuscular mycorrhizal fungi (G+ bacteria) compared with saprotrophic fungi (G− bacteria). These changes drove shifts toward fungal-dominated soil microbial communities and then optimized microbial community structure. Also, manure application increased soil biodiversity (microbial community and enzyme function), indicated by increased Shannon–Wiener diversity. Redundancy analysis indicated that the most possible mechanism of the impacts of different fertilization patterns on soil microbial characteristics may be the mediation of SOC and nutrient (N) availability (especially SOC) in this GVP of China. In conclusion, manure substitution of chemical fertilizer, especially at higher substitution rate, was more efficient for improving soil quality and biological functions.

scholarly journals Effects of Allelochemicals, Soil Enzyme Activities, and Environmental Factors on Rhizosphere Soil Microbial Community of Stellera chamaejasme L. along a Growth-Coverage Gradient

2022 ◽  
Vol 10 (1) ◽  
pp. 158
Author(s):  
Jinan Cheng ◽  
Hui Jin ◽  
Jinlin Zhang ◽  
Zhongxiang Xu ◽  
Xiaoyan Yang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Physicochemical Properties ◽  
Soil Microbial Community ◽  
Enzyme Activities ◽  
Rhizosphere Soil ◽  
Soil Enzyme ◽  
Soil Physicochemical Properties ◽  
Soil Enzyme Activities ◽  
Soil Microbial ◽  
Stellera Chamaejasme

Allelochemicals released from the root of Stellera chamaejasme L. into rhizosphere soil are an important factor for its invasion of natural grasslands. The aim of this study is to explore the interactions among allelochemicals, soil physicochemical properties, soil enzyme activities, and the rhizosphere soil microbial communities of S. chamaejasme along a growth-coverage gradient. High-throughput sequencing was used to determine the microbial composition of the rhizosphere soil sample, and high-performance liquid chromatography was used to detect allelopathic substances. The main fungal phyla in the rhizosphere soil with a growth coverage of 0% was Basidiomycetes, and the other sample plots were Ascomycetes. Proteobacteria and Acidobacteria were the dominant bacterial phyla in all sites. RDA analysis showed that neochamaejasmin B, chamaechromone, and dihydrodaphnetin B were positively correlated with Ascomycota and Glomeromycota and negatively correlated with Basidiomycota. Neochamaejasmin B and chamaechromone were positively correlated with Proteobacteria and Actinobacteria and negatively correlated with Acidobacteria and Planctomycetes. Allelochemicals, soil physicochemical properties, and enzyme activity affected the composition and diversity of the rhizosphere soil microbial community to some extent. When the growth coverage of S. chamaejasme reached the primary stage, it had the greatest impact on soil physicochemical properties and enzyme activities.

scholarly journals Responses of Soil Microbial Community and Enzyme Activities to Shrub Species Artemisia gmelinii in Relation to Varying Rainfall in a Semiarid Land, SW China

2021 ◽  
Vol 9 ◽  
Author(s):  
Laiye Qu ◽  
Bingbing Wang ◽  
Xinyu Zhang ◽  
Minggang Wang
Keyword(s):  
Microbial Community ◽  
Soil Microbial Community ◽  
Enzyme Activities ◽  
Climatic Variation ◽  
Soil Physicochemical Properties ◽  
Shrub Species ◽  
Soil Microbial ◽  
Canopy Soil ◽  
Shrub Canopy ◽  
Semiarid Land

Widely distributed shrubs in drylands can locally alter soil physicochemical properties, which distinguish soil under plant canopy from soil outside the canopy. In the present study, we used a dominant shrub species Artemisia gmelinii in a semiarid land, SW China, to investigate the consequences of “shrub resource islands” for soil microbial communities and enzymatic activities. Such investigation was made at four sites that differed in rates of rainfall to examine how the consequences were altered by variation in the local climate. The results showed that A. gmelinii enhanced fungal abundance but did not influence bacterial abundance, resulting in higher total microbial abundance and fungal-to-bacterial ratio in under-canopy soil compared to outside-canopy soil. Microbial community composition also differed between the two soils, but this difference only occurred at sites of low rainfall. Redundancy analysis revealed that such composition was attributed to variation in soil water content, bulk density, and total phosphorus as a result of shrub canopy and varying rates of rainfall. Activities of hydrolytic enzymes (β-1,4-glucosidase, β-1,4-N-acetylglucosaminidase, alkaline phosphatase, and leucine aminopeptidase) were higher in under-canopy soil than in outside-canopy soil, among which C-acquisition enzyme, β-1,4-glucosidase, and P-acquisition enzyme, alkaline phosphatase, were also higher in the soil of high rainfall. The overall pattern of enzyme activities did not show differences between under- and outside-canopy soils, but it separated the sites of high rate from that of low rates of rainfall. This pattern was primarily driven by variation in soil physicochemical properties rather than variation in soil microbial community, suggesting that the distribution pattern of enzyme activities may be more sensitive to variation in rainfall than to shrub canopy. In conclusion, our study shows that shrub species A. gmelinii can shift the soil microbial community to be fungal-dominant and increase hydrolytic enzyme activities, and such effect may depend on local climatic variation, for example, rainfall changes in the semiarid land. The findings of this study highlight the important roles of shrub vegetation in soil biological functions and the sensitivity of such roles to climatic variation in semiarid ecosystems.

scholarly journals Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China

2021 ◽  
Vol 13 (21) ◽  
pp. 11684
Author(s):  
Li Fan ◽  
Weiping Zhao ◽  
Wendan Feng ◽  
Ping Mo ◽  
Yunlin Zhao ◽  
...  
Keyword(s):  
Microbial Community ◽  
Physicochemical Properties ◽  
Microbial Diversity ◽  
Ecological Restoration ◽  
Soil Microbial Community ◽  
Mining Area ◽  
Soil Restoration ◽  
Soil Physicochemical Properties ◽  
Soil Microbial Diversity ◽  
Soil Microbial

Soil microorganisms play an important role in regulating a variety of ecological functions. In recent years, the research on ecological restoration after mining has made people more aware of the importance of microbial diversity to ecosystem restoration. The present study investigated the effect of ecological restoration on microbial community structure and its relationship with soil physicochemical properties in the Dabaoshan mining area, China. High throughput sequencing technology was used to analyze and compare the microbial community composition of three types of soil (undamaged area, unrestoration area, and ecological restoration area). The contents of organic carbon, total nitrogen, and total phosphorus were 2.38–12.97 g/kg, 0.39–1.62 g/kg, and 0.99–1.51 g/kg, respectively. In different soil states, undamaged area and ecological restoration area were significantly higher than those in unrestoration area. The results showed that the structure of soil microbial community was significantly correlated with soil physicochemical properties, and formations in the repaired and unrepaired soils were different. Operational Taxonomic Unit (OTU) cluster analysis and diversity index analysis showed that soil microbial community changed at phylum and genus levels. The results showed that at the phylum level, all soil samples contained Firmicutes, Proteobacteria, and actinobacteria. Firmicutes and Proteobacteria of the ecological restoration area (ER1, ER2) were the highest in relative abundance compared with other samples, accounting for more than 45%. Proteobacteria and Acidobacteria were the dominant phylum in the undamaged area (UD), accounting for 32.7% and 22.3%, respectively. It can be seen that soil restoration produced a new dominant population, and Proteobacteria showed an absolute competitive advantage in the mining soil.

scholarly journals Responses of soil microbial communities and enzyme activities to nitrogen and phosphorus additions in Chinese fir plantations of subtropical China

2015 ◽  
Vol 12 (13) ◽  
pp. 10359-10387 ◽  
Author(s):  
W. Y. Dong ◽  
X. Y. Zhang ◽  
X. Y. Liu ◽  
X. L. Fu ◽  
F. S. Chen ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Enzyme Activities ◽  
Microbial Community Composition ◽  
Soil Microbial Communities ◽  
Chinese Fir ◽  
Subtropical China ◽  
Nitrogen And Phosphorus ◽  
Soil Microbial ◽  
Nutrient Additions

Abstract. Nitrogen (N) and phosphorus (P) additions to forest ecosystems are known to influence various above-ground properties, such as plant productivity and composition, and below-ground properties, such as soil nutrient cycling. However, our understanding of how soil microbial communities and their functions respond to nutrient additions in subtropical plantations is still not complete. In this study, we added N and P to Chinese fir plantations in subtropical China to examine how nutrient additions influenced soil microbial community composition and enzyme activities. The results showed that most soil microbial properties were responsive to N and/or P additions, but responses often varied depending on the nutrient added and the quantity added. For instance, there were more than 30 % greater increases in the activities of β-Glucosidase (βG) and N-acetyl-β-D-glucosaminidase (NAG) in the treatments that received nutrient additions compared to the control plot, whereas acid phosphatase (aP) activity was always higher (57 and 71 %, respectively) in the P treatment. N and P additions greatly enhanced the PLFA abundanceespecially in the N2P treatment, the bacterial PLFAs (bacPLFAs), fungal PLFAs (funPLFAs) and actinomycic PLFAs (actPLFAs) were about 2.5, 3 and 4 times higher, respectively, than in the CK. Soil enzyme activities were noticeably higher in November than in July, mainly due to seasonal differences in soil moisture content (SMC). βG or NAG activities were significantly and positively correlated with microbial PLFAs. There were also significant relationships between gram-positive (G+) bacteria and all three soil enzymes. These findings indicate that G+ bacteria is the most important microbial community in C, N, and P transformations in Chinese fir plantations, and that βG and NAG would be useful tools for assessing the biogeochemical transformation and metabolic activity of soil microbes. We recommend combined additions of N and P fertilizer to promote soil fertility and microbial activity in this kind of plantation.

scholarly journals STRUKTUR KOMUNITAS MIKROBA TANAH DAN IMPLIKASINYA DALAM MEWUJUDKAN SISTEM PERTANIAN BERKELANJUTAN

el–Hayah ◽  
2012 ◽  
Vol 1 (4) ◽  
Author(s):  
Prihastuti Prihastuti
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
Microbial Communities ◽  
Soil Microbial Community ◽  
Soil Microbial Communities ◽  
Organic Soil ◽  
Plant Type ◽  
Soil Microbial Community Structure ◽  
Soil Microbial ◽  
Wide Range

<p>Soils are made up of organic and an organic material. The organic soil component contains all the living creatures in the soil and the dead ones in various stages of decomposition.  Biological activity in soil helps to recycle nutrients, decompose organic matter making nutrient available for plant uptake, stabilize humus, and form soil particles.<br />The extent of the diversity of microbial in soil is seen to be critical to the maintenance of soil health and quality, as a wide range of microbial is involved in important soil functions.  That ecologically managed soils have a greater quantity and diversity of soil microbial. The two main drivers of soil microbial community structure, i.e., plant type and soil type, are thought to exert their function in a complex manner. The fact that in some situations the soil and in others the plant type is the key factor determining soil microbial diversity is related to their complexity of the microbial interactions in soil, including interactions between microbial and soil and microbial and plants. <br />The basic premise of organic soil stewardship is that all plant nutrients are present in the soil by maintaining a biologically active soil environment. The diversity of microbial communities has on ecological function and resilience to disturbances in soil ecosystems. Relationships are often observed between the extent of microbial diversity in soil, soil and plant quality and ecosystem sustainability. Agricultural management can be directed toward maximizing the quality of the soil microbial community in terms of disease suppression, if it is possible to shift soil microbial communities.</p><p>Keywords: structure, microbial, implication, sustainable agriculture<br /><br /></p>

Distinct assembly processes and determinants of soil microbial communities between farmland and grassland in arid and semiarid areas

2021 ◽  
Author(s):  
Aiai Xu ◽  
Jie Liu ◽  
Zhiying Guo ◽  
Changkun Wang ◽  
Kai Pan ◽  
...  
Keyword(s):  
Microbial Community ◽  
Variable Selection ◽  
Microbial Communities ◽  
Soil Microbial Community ◽  
Environmental Gradient ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Semiarid Areas ◽  
Arid And Semiarid Areas ◽  
Arid And Semiarid

It is critical to identify the assembly processes and determinants of soil microbial communities to better predict soil microbial responses to environmental change in arid and semiarid areas. Here, soils from 16 grassland-only, 9 paired grassland and farmland, and 16 farmland-only sites were collected across the central Inner Mongolia Plateau covering a steep environmental gradient. Through analyzing the paired samples, we discovered that land uses had strong effects on soil microbial communities, but weak effects on their assembly processes. For all samples, although no environmental variables were significantly correlated with the net relatedness index (NRI), both the nearest taxon index (NTI) and the β-nearest taxon index (βNTI) were most related to mean annual precipitation (MAP). With the increase of MAP, soil microbial taxa at the tips of the phylogenetic tree were more clustered, and the contribution of determinism increased. Determinism (48.6%), especially variable selection (46.3%), and stochasticity (51.4%) were almost equal in farmland, while stochasticity (75.0%) was dominant in grassland. Additionally, Mantel tests and redundancy analyses (RDA) revealed that the main determinants of soil microbial community structure were MAP in grassland, but mean annual temperature (MAT) in farmland. MAP and MAT were also good predictors of the community composition (the top 200 dominant OTUs) in grassland and farmland, respectively. Collectively, in arid and semiarid areas, soil microbial communities were more sensitive to environmental change in farmland than in grassland, and unlike the major impact of MAP on grassland microbial communities, MAT was the primary driver of farmland microbial communities. Importance As one of the most diverse organisms, soil microbes play indispensable roles in many ecological processes in arid and semiarid areas with limited macrofaunal and plant diversity, yet the mechanisms underpinning soil microbial community are not fully understood. In this study, soil microbial communities were investigated along a 500 km transect covering a steep environmental gradient across farmland and grassland in the areas. The results showed that precipitation was the main factor mediating the assembly processes. Determinism was more influential in farmland, and variable selection of farmland was twice that of grassland. Temperature mainly drove farmland microbial communities, while precipitation mainly affected grassland microbial communities. These findings provide new information about the assembly processes and determinants of soil microbial communities in arid and semiarid areas, consequently improving the predictability of the community dynamics, which have implications for sustaining soil microbial diversity and ecosystem functioning, particularly under global climate change conditions.

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