scholarly journals The dynamics of centromere motion through the metaphase-to-anaphase transition reveal a centromere separation order

2019 ◽  
Author(s):  
Jonathan W. Armond ◽  
Katie L. Dale ◽  
Nigel J. Burroughs ◽  
Andrew D. McAinsh ◽  
Elina Vladimirou
Keyword(s):  
Chromosome Segregation ◽  
High Speed ◽  
Daughter Cell ◽  
Mitotic Chromosome ◽  
Low Frequency ◽  
The Other ◽  
Bayesian Modelling ◽  
High Speed Imaging ◽  
Chromosome Dynamics ◽  
Sister Chromatids

AbstractDuring cell division, chromosomes align at the equator of the cell before sister chromatids separate to move to each daughter cell during anaphase. We use high-speed imaging, Bayesian modelling and quantitative analysis to examine the regulation of centromere dynamics through the metaphase-to-anaphase transition. We find that, contrary to the apparent instantaneous separation seen in low-frequency imaging, centromeres separate asynchronously over 1-2 minutes. The timing of separations negatively correlates with the centromere intersister distance during metaphase, which could potentially be explained by variable amounts of cohesion at centromeres. Depletion of condensin I increases this asynchrony. Depletion of condensin II, on the other hand, abolishes centromere metaphase oscillations and impairs centromere speed in anaphase. These results suggest that condensin complexes have broader direct roles in mitotic chromosome dynamics than previously believed and may be crucial for the regulation of chromosome segregation.

scholarly journals Condensin’s ATPase Machinery Drives and Dampens Mitotic Chromosome Condensation

2017 ◽  
Author(s):  
Ahmed M.O. Elbatsh ◽  
Jonne A. Raaijmakers ◽  
Robin H. van der Weide ◽  
Jelmi Kuit de Bos ◽  
Hans Teunissen ◽  
...  
Keyword(s):  
Chromosome Segregation ◽  
Mitotic Chromosome ◽  
Chromosome Condensation ◽  
The Other ◽  
Condensation Process ◽  
Total Absence ◽  
Sister Chromatids ◽  
Mitotic Chromosome Condensation ◽  
Do So

ABSTRACTChromosome condensation by condensin is essential for faithful chromosome segregation. Metazoans have two complexes, named condensin I and II. Both are thought to act by creating looped structures in DNA, but how they do so is unknown. Condensin’s SMC subunits together form a composite ATPase with two pseudo-symmetric ATPase sites. We reveal that these sites have opposite functions in the condensation process. One site drives condensation, while the other site rather has a dampening function. Mutation of this dampener site hyperactivates both condensin I and II complexes. We find that hyperactive condensin I efficiently shortens chromosomes in the total absence of condensin II. The two complexes form loops with different lengths, and specifically condensin II is key to the decatenation of sister chromatids and the formation of a straight chromosomal axis.

scholarly journals Vocal state change through laryngeal development

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yisi S. Zhang ◽  
Daniel Y. Takahashi ◽  
Diana A. Liao ◽  
Asif A. Ghazanfar ◽  
Coen P. H. Elemans
Keyword(s):  
Motor Control ◽  
High Speed ◽  
Neural Circuits ◽  
Low Frequency ◽  
Vocal Folds ◽  
Vocal Development ◽  
High Speed Imaging ◽  
State Changes ◽  
Material Tests ◽  
Excised Larynx

Abstract Across vertebrates, progressive changes in vocal behavior during postnatal development are typically attributed solely to developing neural circuits. How the changing body influences vocal development remains unknown. Here we show that state changes in the contact vocalizations of infant marmoset monkeys, which transition from noisy, low frequency cries to tonal, higher pitched vocalizations in adults, are caused partially by laryngeal development. Combining analyses of natural vocalizations, motorized excised larynx experiments, tensile material tests and high-speed imaging, we show that vocal state transition occurs via a sound source switch from vocal folds to apical vocal membranes, producing louder vocalizations with higher efficiency. We show with an empirically based model of descending motor control how neural circuits could interact with changing laryngeal dynamics, leading to adaptive vocal development. Our results emphasize the importance of embodied approaches to vocal development, where exploiting biomechanical consequences of changing material properties can simplify motor control, reducing the computational load on the developing brain.

Effect of Low-Frequency Modulation on Deformation and Material Flow in Cutting of Metals

2015 ◽  
Vol 138 (1) ◽  
Author(s):  
Ho Yeung ◽  
Yang Guo ◽  
James B. Mann ◽  
W. Dale Compton ◽  
Srinivasan Chandrasekar
Keyword(s):  
Frequency Modulation ◽  
Chip Formation ◽  
Material Flow ◽  
High Speed ◽  
Surface Deformation ◽  
Low Frequency ◽  
High Speed Imaging ◽  
Machining Processes ◽  
Asperity Contacts ◽  
2D System

The deformation field, material flow, and mechanics of chip separation in cutting of metals with superimposed low-frequency modulation (<1000 Hz) are characterized at the mesoscale using high-speed imaging and particle image velocimetry (PIV). The two-dimensional (2D) system studied involves a sharp-wedge sliding against the workpiece to remove material, also reminiscent of asperity contacts in sliding. A unique feature of the study is in situ mapping of material flow at high resolution using strain fields and streaklines and simultaneous measurements of tool motions and forces, such that instantaneous forces and kinematics can be overlaid onto the chip formation process. The significant reductions in specific energy obtained when cutting with modulation are shown to be a consequence of discrete chip formation with reduced strain levels. This strain reduction is established by direct measurements of deformation fields. The results have implications for enhancing sustainability of machining processes and understanding surface deformation and material removal in wear processes.

Application of Proper Orthogonal Decomposition to High Speed Imaging for the Study of Combustion Oscillations

2017 ◽  
Author(s):  
Siddhartha Gadiraju ◽  
Suhyeon Park ◽  
David Gomez-Ramirez ◽  
Srinath V. Ekkad ◽  
K. Todd Lowe ◽  
...  
Keyword(s):  
Proper Orthogonal Decomposition ◽  
High Speed ◽  
Equivalence Ratio ◽  
Flame Structure ◽  
Low Frequency ◽  
Orthogonal Decomposition ◽  
Pressure Measurements ◽  
High Speed Imaging ◽  
Flame Structures ◽  
Proper Orthogonal

The flame structure and characteristics generated by an industrial low emission, lean premixed, fuel swirl nozzle were analyzed for understanding combustion oscillations. The experimental facility is located at the Advanced Propulsion and Power Laboratory (APPL) at Virginia Tech. The experiments were carried out in a model optical can combustor operating at atmospheric pressures. Low-frequency oscillations (<100 Hz) were observed during the reaction as opposed to no reaction, cold flow test cases. The objective of this paper is to understand the frequency and magnitude of oscillations due to combustion using high-speed imaging and associate them with corresponding structure or feature of the flame. Flame images were obtained using a Photron Fastcam SA4 high-speed camera at 500 frames per second. The experiments were conducted at equivalence ratios of 0.65, 0.75; different Reynolds numbers of 50K, 75K; and three pilot fuel to main fuel ratios of 0%, 3%, 6%. In this study, Reynolds number was based on the throat diameter of the fuel nozzle. Since the time averaged flame images are not adequate representation of the flame structures, proper orthogonal decomposition (POD) was applied to the flame images to extract the dominant features. The spatiotemporal dynamics of the images can be decomposed into their constituent modes of maximum spatial variance using POD so that the dominant features of the flame can be observed. The frequency of the dominant flame structures, as captured by the POD modes of the flame acquisitions, were consistent with pressure measurements taken at the exit of the combustor. Thus, the oscillations due to combustion can be visualized using POD. POD was further applied to high-speed images taken during instabilities. Specifically, the instabilities discussed in this paper are those encountered when the equivalence ratio is reduced to the levels approaching lean blowout (LBO). As the equivalence ratio is reduced to near blowout regime, it triggers low-frequency high amplitude instabilities. These low-frequency instabilities are visible as the flapping of the flame. The frequencies of the dominant POD modes are consistent with pressure measurements recorded during these studies.

scholarly journals Condensin restructures chromosomes in preparation for meiotic divisions

2004 ◽  
Vol 167 (4) ◽  
pp. 613-625 ◽  
Author(s):  
Raymond C. Chan ◽  
Aaron F. Severson ◽  
Barbara J. Meyer
Keyword(s):  
Caenorhabditis Elegans ◽  
Chromosome Segregation ◽  
Germ Cells ◽  
Protein Complex ◽  
Mitotic Chromosome ◽  
Meiotic Chromosome ◽  
Ordered Structure ◽  
Homologous Chromosomes ◽  
Sister Chromatids ◽  
Chromosome Resolution

The production of haploid gametes from diploid germ cells requires two rounds of meiotic chromosome segregation after one round of replication. Accurate meiotic chromosome segregation involves the remodeling of each pair of homologous chromosomes around the site of crossover into a highly condensed and ordered structure. We showed that condensin, the protein complex needed for mitotic chromosome compaction, restructures chromosomes during meiosis in Caenorhabditis elegans. In particular, condensin promotes both meiotic chromosome condensation after crossover recombination and the remodeling of sister chromatids. Condensin helps resolve cohesin-independent linkages between sister chromatids and alleviates recombination-independent linkages between homologues. The safeguarding of chromosome resolution by condensin permits chromosome segregation and is crucial for the formation of discrete, individualized bivalent chromosomes.

scholarly journals Nse1, Nse2, and a Novel Subunit of the Smc5-Smc6 Complex, Nse3, Play a Crucial Role in Meiosis

2004 ◽  
Vol 15 (11) ◽  
pp. 4866-4876 ◽  
Author(s):  
Stephanie Pebernard ◽  
W. Hayes McDonald ◽  
Yelena Pavlova ◽  
John R. Yates ◽  
Michael N. Boddy
Keyword(s):  
Homologous Recombination ◽  
Chromosome Segregation ◽  
Replication Fork ◽  
Mitotic Chromosome ◽  
Nuclear Protein ◽  
Genetic Interactions ◽  
Cellular Resistance ◽  
Spore Viability ◽  
Sister Chromatids ◽  
Structural Maintenance Of Chromosomes

The structural maintenance of chromosomes (SMC) family of proteins play key roles in the organization, packaging, and repair of chromosomes. Cohesin (Smc1+3) holds replicated sister chromatids together until mitosis, condensin (Smc2+4) acts in chromosome condensation, and Smc5+6 performs currently enigmatic roles in DNA repair and chromatin structure. The SMC heterodimers must associate with non-SMC subunits to perform their functions. Using both biochemical and genetic methods, we have isolated a novel subunit of the Smc5+6 complex, Nse3. Nse3 is an essential nuclear protein that is required for normal mitotic chromosome segregation and cellular resistance to a number of genotoxic agents. Epistasis with Rhp51 (Rad51) suggests that like Smc5+6, Nse3 functions in the homologous recombination based repair of DNA damage. We previously identified two non-SMC subunits of Smc5+6 called Nse1 and Nse2. Analysis of nse1-1, nse2-1, and nse3-1 mutants demonstrates that they are crucial for meiosis. The Nse1 mutant displays meiotic DNA segregation and homologous recombination defects. Spore viability is reduced by nse2-1 and nse3-1, without affecting interhomolog recombination. Finally, genetic interactions shared by the nse mutants suggest that the Smc5+6 complex is important for replication fork stability.

scholarly journals What is the optimal light source for optical mapping using voltage- and calcium-sensitive dyes?

2020 ◽  
pp. 599-607
Author(s):  
V Olejnickova ◽  
D Sedmera
Keyword(s):  
Light Source ◽  
Optical System ◽  
High Speed ◽  
Optical Mapping ◽  
The Other ◽  
Mouse Heart ◽  
Xenon Lamp ◽  
Light Sources ◽  
High Speed Imaging ◽  
Advantages And Disadvantages

Optical mapping is a fluorescence-based physiological method to image spreading of action potential in excitable tissues, such as the heart and central nervous system. Because of the requirements for high speed imaging in low light conditions, highly sensitive high-speed cameras together with an optical system with maximum photon efficiency are required. While the optimization of these two components is relatively straightforward, the choice of the perfect light source is less simple; depending on the other (usually fixed) components, various parameters may acquire different weight in decision-making process. Here we describe the rationale for building an optical mapping setup and consider the relative advantages and disadvantages of three different commonly available light sources: mercury vapor lamp (HBO), xenon lamp (XBO), and light emitting diode (LED). Using the same optical system (fluorescence macroscope) and high-speed camera (Ultima L), we have tested each of the sources for its ability to provide bright and even illumination of the field of view and measured its temporal fluctuations in intensity. Then we used each in the actual optical mapping experiment using isolated, perfused adult mouse heart or chick embryonic heart to determine the actual signal to noise ratio at various acquisition rates. While the LED sources have undergone significant improvements in the recent past, the other alternatives may still surpass them in some parameters, so they may not be the automatic number one choice for every application.

Experimental Measurement of In-Plane Rolling Nonpneumatic Tire Vibrations Using High-Speed Imaging

2019 ◽  
Vol 47 (3) ◽  
pp. 196-210
Author(s):  
Meghashyam Panyam ◽  
Beshah Ayalew ◽  
Timothy Rhyne ◽  
Steve Cron ◽  
John Adcox
Keyword(s):  
High Speed ◽  
Image Correlation ◽  
Dynamic Mode Decomposition ◽  
Dynamic Mode ◽  
High Speed Imaging ◽  
Correlation Algorithm ◽  
Mode Decomposition ◽  
Series Of Experiments ◽  
Plane Deformations ◽  
Dominant Frequencies

ABSTRACT This article presents a novel experimental technique for measuring in-plane deformations and vibration modes of a rotating nonpneumatic tire subjected to obstacle impacts. The tire was mounted on a modified quarter-car test rig, which was built around one of the drums of a 500-horse power chassis dynamometer at Clemson University's International Center for Automotive Research. A series of experiments were conducted using a high-speed camera to capture the event of the rotating tire coming into contact with a cleat attached to the surface of the drum. The resulting video was processed using a two-dimensional digital image correlation algorithm to obtain in-plane radial and tangential deformation fields of the tire. The dynamic mode decomposition algorithm was implemented on the deformation fields to extract the dominant frequencies that were excited in the tire upon contact with the cleat. It was observed that the deformations and the modal frequencies estimated using this method were within a reasonable range of expected values. In general, the results indicate that the method used in this study can be a useful tool in measuring in-plane deformations of rolling tires without the need for additional sensors and wiring.

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