Functional characterization of RNase H1 proteins in Arabidopsis thaliana

ABSTRACTRNase H1 is an endonuclease specific towards RNA:DNA hybrids. Members of this protein family are present in most living organisms and are essential for removing RNA that base pairs with DNA. It prevents detrimental effects of RNA:DNA hybrids and is involved in several biological processes. We show that Arabidopsis thaliana contains four RNase H1-like proteins originating from two gene duplication events and alternative splicing. These proteins have the canonical RNase H1 activity, which requires at least four ribonucleotides for activity. Two of those proteins are nuclear, one is localized to mitochondria and one to plastids. While the nuclear RNases H1 are dispensable, the presence of at least one organellar RNase H1 is required for embryonic development. The plastid protein RNH1C affects plastid DNA copy number and sensitivity to hydroxyurea. This indicates that three genomes present in each plant cell are served by at least one specialized RNase H1 protein.

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Faculty Opinions recommendation of Transport of UDP-galactose in plants. Identification and functional characterization of AtUTr1, an Arabidopsis thaliana UDP-galactos/UDP-glucose transporter.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1007601.96055 ◽

2002 ◽

Author(s):

Paul Dupree

Keyword(s):

Arabidopsis Thaliana ◽

Glucose Transporter ◽

Functional Characterization

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Functional Characterization of DEAD-Box RNA Helicases in Arabidopsis thaliana under Abiotic Stress Conditions

Plant and Cell Physiology ◽

10.1093/pcp/pcn125 ◽

2008 ◽

Vol 49 (10) ◽

pp. 1563-1571 ◽

Cited By ~ 57

Author(s):

Jin Sun Kim ◽

Kyung Ae Kim ◽

Tae Rin Oh ◽

Chul Min Park ◽

Hunseung Kang

Keyword(s):

Arabidopsis Thaliana ◽

Abiotic Stress ◽

Functional Characterization ◽

Stress Conditions ◽

Rna Helicases ◽

Dead Box

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Bioinformatic and functional characterization of the basic peroxidase 72 from Arabidopsis thaliana involved in lignin biosynthesis

Planta ◽

10.1007/s00425-013-1865-5 ◽

2013 ◽

Vol 237 (6) ◽

pp. 1599-1612 ◽

Cited By ~ 63

Author(s):

Joaquín Herrero ◽

Francisco Fernández-Pérez ◽

Tatiana Yebra ◽

Esther Novo-Uzal ◽

Federico Pomar ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Functional Characterization ◽

Lignin Biosynthesis

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Genome-wide identification of soybean Shaker K+ channel gene family and functional characterization of GmAKT1 in transgenic Arabidopsis thaliana under salt and drought stress

Journal of Plant Physiology ◽

10.1016/j.jplph.2021.153529 ◽

2021 ◽

pp. 153529

Author(s):

Chen Feng ◽

Chengming He ◽

Yifan Wang ◽

Hehan Xu ◽

Keheng Xu ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Drought Stress ◽

Gene Family ◽

Transgenic Arabidopsis ◽

Functional Characterization ◽

K Channel ◽

Channel Gene ◽

Genome Wide ◽

Salt And Drought Stress

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Functional characterization of wheat myo-inositol oxygenase promoter under different abiotic stress conditions in Arabidopsis thaliana

Biotechnology Letters ◽

10.1007/s10529-020-02967-1 ◽

2020 ◽

Vol 42 (10) ◽

pp. 2035-2047

Author(s):

Anshu Alok ◽

Jaspreet Kaur ◽

Siddharth Tiwari

Keyword(s):

Arabidopsis Thaliana ◽

Abiotic Stress ◽

Functional Characterization ◽

Stress Conditions

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Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana

Plant and Cell Physiology ◽

10.1093/pcp/pcaa040 ◽

2020 ◽

Vol 61 (6) ◽

pp. 1107-1119

Author(s):

Jan Kuciński ◽

Sebastian Chamera ◽

Aleksandra Kmera ◽

M Jordan Rowley ◽

Sho Fujii ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Embryonic Development ◽

Evolutionary History ◽

Common Ancestor ◽

Normal Growth ◽

Plastid Dna ◽

Growth Conditions ◽

Replicative Stress ◽

The Common ◽

Rna:Dna Hybrids

Abstract RNase H1 is an endonuclease specific toward the RNA strand of RNA:DNA hybrids. Members of this protein family are present in most living organisms and are essential for removing RNA that base pairs with DNA. It prevents detrimental effects of RNA:DNA hybrids and is involved in several biological processes. Arabidopsis thaliana has been previously shown to contain three genes encoding RNase H1 proteins that localize to three distinct cellular compartments. We show that these genes originate from two gene duplication events. One occurred in the common ancestor of dicots and produced nuclear and organellar RNase H1 paralogs. Second duplication occurred in the common ancestor of Brassicaceae and produced mitochondrial- and plastid-localized proteins. These proteins have the canonical RNase H1 activity, which requires at least four ribonucleotides for endonucleolytic digestion. Analysis of mutants in the RNase H1 genes revealed that the nuclear RNH1A and mitochondrial RNH1B are dispensable for development under normal growth conditions. However, the presence of at least one organellar RNase H1 (RNH1B or RNH1C) is required for embryonic development. The plastid-localized RNH1C affects plastid DNA copy number and sensitivity to replicative stress. Our results present the evolutionary history of RNH1 proteins in A. thaliana, demonstrate their canonical RNase H1 activity and indicate their role in early embryonic development.

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