scholarly journals Relationship between simultaneously recorded spiking activity and fluorescence signal in GCaMP6 transgenic mice

2019 ◽  
Author(s):  
Lawrence Huang ◽  
Ulf Knoblich ◽  
Peter Ledochowitsch ◽  
Jérôme Lecoq ◽  
R. Clay Reid ◽  
...  
Keyword(s):  
Calcium Imaging ◽  
Pyramidal Neurons ◽  
False Positive Rate ◽  
Ground Truth ◽  
Fluorescence Signal ◽  
Spatiotemporal Resolution ◽  
Detection Rates ◽  
Single Spike ◽  
Positive Rate

AbstractTwo-photon calcium imaging is often used with genetically encoded calcium indicators (GECIs) to investigate neural dynamics, but the relationship between fluorescence and action potentials (spikes) remains unclear. Pioneering work linked electrophysiology and calcium imaging in vivo with viral GECI expression, albeit in a small number of cells. Here we characterized the spikefluorescence transfer function in vivo of 91 layer 2/3 pyramidal neurons in primary visual cortex in four transgenic mouse lines expressing GCaMP6s or GCaMP6f. We found that GCaMP6s cells have spike-triggered fluorescence responses of larger amplitude, lower variability and greater single-spike detectability than GCaMP6f. Mean single-spike detection rates at high spatiotemporal resolution measured in our data was >70% for GCaMP6s and ~40-50% for GCaMP6f (at 5% false positive rate). These rates are estimated to decrease to 25-35% for GCaMP6f under generally used population imaging conditions. Our ground-truth dataset thus supports more refined inference of neuronal activity from calcium imaging.

Predicting Fetal Chromosome Anomalies in the First Trimester Using Pregnancy Associated Plasma Protein-A: A Comparison of Statistical Methods

1993 ◽  
Vol 32 (02) ◽  
pp. 175-179 ◽  
Author(s):  
B. Brambati ◽  
T. Chard ◽  
J. G. Grudzinskas ◽  
M. C. M. Macintosh
Keyword(s):  
Logistic Regression ◽  
General Population ◽  
Likelihood Ratio ◽  
False Positive ◽  
False Positive Rate ◽  
Ratio Method ◽  
Detection Rates ◽  
Gaussian Distributions ◽  
Positive Rate ◽  
Likelihood Ratio Method

Abstract:The analysis of the clinical efficiency of a biochemical parameter in the prediction of chromosome anomalies is described, using a database of 475 cases including 30 abnormalities. A comparison was made of two different approaches to the statistical analysis: the use of Gaussian frequency distributions and likelihood ratios, and logistic regression. Both methods computed that for a 5% false-positive rate approximately 60% of anomalies are detected on the basis of maternal age and serum PAPP-A. The logistic regression analysis is appropriate where the outcome variable (chromosome anomaly) is binary and the detection rates refer to the original data only. The likelihood ratio method is used to predict the outcome in the general population. The latter method depends on the data or some transformation of the data fitting a known frequency distribution (Gaussian in this case). The precision of the predicted detection rates is limited by the small sample of abnormals (30 cases). Varying the means and standard deviations (to the limits of their 95% confidence intervals) of the fitted log Gaussian distributions resulted in a detection rate varying between 42% and 79% for a 5% false-positive rate. Thus, although the likelihood ratio method is potentially the better method in determining the usefulness of a test in the general population, larger numbers of abnormal cases are required to stabilise the means and standard deviations of the fitted log Gaussian distributions.

scholarly journals High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
...  
Keyword(s):  
Blood Flow ◽  
High Speed ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Microscopy ◽  
Fluorescence Signal ◽  
Imaging Method ◽  
Spatiotemporal Resolution ◽  
Two Photon

AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.

scholarly journals Night-Time Vehicle Sensing in Far Infrared Image with Deep Learning

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Hai Wang ◽  
Yingfeng Cai ◽  
Xiaobo Chen ◽  
Long Chen
Keyword(s):  
Detection Rate ◽  
False Positive Rate ◽  
Infrared Image ◽  
Far Infrared ◽  
Night Vision ◽  
Infrared Sensors ◽  
Night Time ◽  
Detection Rates ◽  
Positive Rate ◽  
And Performance

The use of night vision systems in vehicles is becoming increasingly common. Several approaches using infrared sensors have been proposed in the literature to detect vehicles in far infrared (FIR) images. However, these systems still have low vehicle detection rates and performance could be improved. This paper presents a novel method to detect vehicles using a far infrared automotive sensor. Firstly, vehicle candidates are generated using a constant threshold from the infrared frame. Contours are then generated by using a local adaptive threshold based on maximum distance, which decreases the number of processing regions for classification and reduces the false positive rate. Finally, vehicle candidates are verified using a deep belief network (DBN) based classifier. The detection rate is 93.9% which is achieved on a database of 5000 images and video streams. This result is approximately a 2.5% improvement on previously reported methods and the false detection rate is also the lowest among them.

Watch For Failing Objects: What Inappropriate Compliance Reveals About Shared Mental Models In Autonomous Cars

2021 ◽  
Vol 65 (1) ◽  
pp. 643-647
Author(s):  
Yosef S. Razin ◽  
Jack Gale ◽  
Jiaojiao Fan ◽  
Jaznae’ Smith ◽  
Karen M. Feigh
Keyword(s):  
Mental Models ◽  
Mental Model ◽  
False Positive Rate ◽  
Ground Truth ◽  
True Positive Rate ◽  
Shared Mental Models ◽  
Shared Mental Model ◽  
Autonomous Cars ◽  
Positive Rate ◽  
Dispositional Factors

This paper evaluates Banks et al.’s Human-AI Shared Mental Model theory by examining how a self-driving vehicle’s hazard assessment facilitates shared mental models. Participants were asked to affirm the vehicle’s assessment of road objects as either hazards or mistakes in real-time as behavioral and subjective measures were collected. The baseline performance of the AI was purposefully low (<50%) to examine how the human’s shared mental model might lead to inappropriate compliance. Results indicated that while the participant true positive rate was high, overall performance was reduced by the large false positive rate, indicating that participants were indeed being influenced by the Al’s faulty assessments, despite full transparency as to the ground-truth. Both performance and compliance were directly affected by frustration, mental, and even physical demands. Dispositional factors such as faith in other people’s cooperativeness and in technology companies were also significant. Thus, our findings strongly supported the theory that shared mental models play a measurable role in performance and compliance, in a complex interplay with trust.

scholarly journals Fiber photometry does not reflect spiking activity in the striatum

2021 ◽  
Author(s):  
Alex A. Legaria ◽  
Julia A. Licholai ◽  
Alexxai V. Kravitz
Keyword(s):  
Neuronal Activity ◽  
Calcium Imaging ◽  
Brain Activity ◽  
Neural Population ◽  
Fluorescence Signal ◽  
Calcium Signals ◽  
Cellular Events ◽  
Neural Populations ◽  
Spiking Activity

AbstractFiber photometry recordings are commonly used as a proxy for neuronal activity, based on the assumption that increases in bulk calcium fluorescence reflect increases in spiking of the underlying neural population. However, this assumption has not been adequately tested. Here, using endoscopic calcium imaging in the striatum we report that the bulk fluorescence signal correlates weakly with somatic calcium signals, suggesting that this signal does not reflect spiking activity, but may instead reflect subthreshold changes in neuropil calcium. Consistent with this suggestion, the bulk fluorescence photometry signal correlated strongly with neuropil calcium signals extracted from these same endoscopic recordings. We further confirmed that photometry did not reflect striatal spiking activity with simultaneous in vivo extracellular electrophysiology and fiber photometry recordings in awake behaving mice. We conclude that the fiber photometry signal should not be considered a proxy for spiking activity in neural populations in the striatum.Significance statementFiber photometry is a technique for recording brain activity that has gained popularity in recent years due to it being an efficient and robust way to record the activity of genetically defined populations of neurons. However, it remains unclear what cellular events are reflected in the photometry signal. While it is often assumed that the photometry signal reflects changes in spiking of the underlying cell population, this has not been adequately tested. Here, we processed calcium imaging recordings to extract both somatic and non-somatic components of the imaging field, as well as a photometry signal from the whole field. Surprisingly, we found that the photometry signal correlated much more strongly with the non-somatic than the somatic signals. This suggests that the photometry signal most strongly reflects subthreshold changes in calcium, and not spiking. We confirmed this point with simultaneous fiber photometry and extracellular spiking recordings, again finding that photometry signals relate poorly to spiking in the striatum. Our results may change interpretations of studies that use fiber photometry as an index of spiking output of neural populations.

Association Rule-Mining-Based Intrusion Detection System With Entropy-Based Feature Selection

2020 ◽  
pp. 1-24
Author(s):  
Devaraju Sellappan ◽  
Ramakrishnan Srinivasan
Keyword(s):  
Intrusion Detection ◽  
Association Rule ◽  
Intrusion Detection System ◽  
Association Rule Mining ◽  
Detection System ◽  
False Positive Rate ◽  
Rule Mining ◽  
Detection Rates ◽  
Mining Algorithm ◽  
Positive Rate

Intrusion detection system (IDSs) are important to industries and organizations to solve the problems of networks, and various classifiers are used to classify the activity as malicious or normal. Today, the security has become a decisive part of any industrial and organizational information system. This chapter demonstrates an association rule-mining algorithm for detecting various network intrusions. The KDD dataset is used for experimentation. There are three input features classified as basic features, content features, and traffic features. There are several attacks are present in the dataset which are classified into Denial of Service (DoS), Probe, Remote to Local (R2L), and User to Root (U2R). The proposed method gives significant improvement in the detection rates compared with other methods. Association rule mining algorithm is proposed to evaluate the KDD dataset and dynamic data to improve the efficiency, reduce the false positive rate (FPR) and provides less time for processing.

Impact of Including or Removing Nuchal Translucency Measurement on the Detection and False-Positive Rates of First-Trimester Down Syndrome Screening

2015 ◽  
Vol 40 (3) ◽  
pp. 214-218 ◽  
Author(s):  
Emmanuel Spaggiari ◽  
Isabelle Czerkiewicz ◽  
Corinne Sault ◽  
Sophie Dreux ◽  
Armelle Galland ◽  
...  
Keyword(s):  
Down Syndrome ◽  
False Positive ◽  
False Positive Rate ◽  
Method Comparison ◽  
First Trimester ◽  
Nuchal Translucency ◽  
Routine Practice ◽  
Detection Rates ◽  
Significant Difference ◽  
Positive Rate

Introduction: First-trimester Down syndrome (DS) screening combining maternal age, serum markers (pregnancy-associated plasma protein-A and beta-human chorionic gonadotropin) and nuchal translucency (NT) gives an 85% detection rate for a 5% false-positive rate. These results largely depend on quality assessment of biochemical markers and of NT. In routine practice, despite an ultrasound quality control organization, NT images can be considered inadequate. The aim of the study was to evaluate the consequences for risk calculation when NT measurement is not taken into account. Material and Method: Comparison of detection and false-positive rates of first-trimester DS screening (PerkinElmer, Turku, Finland), with and without NT, based on a retrospective study of 117,126 patients including 274 trisomy 21-affected fetuses. NT was measured by more than 3,000 certified sonographers. Results: There was no significant difference in detection rates between the two strategies including or excluding NT measurement (86.7 vs. 81.8%). However, there was a significant difference in the false-positive rates (2.23 vs. 9.97%, p < 0.001). Discussion: Sonographers should be aware that removing NT from combined first-trimester screening would result in a 5-fold increase in false-positive rate to maintain the expected detection rates. This should be an incentive for maintaining quality in NT measurement.

scholarly journals Real-Time Surgical Problem Detection and Instrument Tracking in Cataract Surgery

2020 ◽  
Vol 9 (12) ◽  
pp. 3896
Author(s):  
Shoji Morita ◽  
Hitoshi Tabuchi ◽  
Hiroki Masumoto ◽  
Hirotaka Tanabe ◽  
Naotake Kamiura
Keyword(s):  
Cataract Surgery ◽  
Real Time ◽  
False Positive Rate ◽  
Surgical Techniques ◽  
Surgical Skill ◽  
Skill Levels ◽  
Detection Rates ◽  
Surgical Methods ◽  
Instrument Tracking ◽  
Positive Rate

Surgical skill levels of young ophthalmologists tend to be instinctively judged by ophthalmologists in practice, and hence a stable evaluation is not always made for a single ophthalmologist. Although it has been said that standardizing skill levels presents difficulty as surgical methods vary greatly, approaches based on machine learning seem to be promising for this objective. In this study, we propose a method for displaying the information necessary to quantify the surgical techniques of cataract surgery in real-time. The proposed method consists of two steps. First, we use InceptionV3, an image classification network, to extract important surgical phases and to detect surgical problems. Next, one of the segmentation networks, scSE-FC-DenseNet, is used to detect the cornea and the tip of the surgical instrument and the incisional site in the continuous curvilinear capsulorrhexis, a particularly important phase in cataract surgery. The first and second steps are evaluated in terms of the area under curve (i.e., AUC) of the figure of the true positive rate versus (1—false positive rate) and the intersection over union (i.e., IoU) obtained by the ground truth and prediction associated with the region of interest. As a result, in the first step, the network was able to detect surgical problems with an AUC of 0.97. In the second step, the detection rate of the cornea was 99.7% when the IoU was 0.8 or more, and the detection rates of the tips of the forceps and the incisional site were 86.9% and 94.9% when the IoU was 0.1 or more, respectively. It was thus expected that the proposed method is one of the basic techniques to achieve the standardization of surgical skill levels.

Association Rule-Mining-Based Intrusion Detection System With Entropy-Based Feature Selection

2021 ◽  
pp. 183-206
Author(s):  
Devaraju Sellappan ◽  
Ramakrishnan Srinivasan
Keyword(s):  
Intrusion Detection ◽  
Association Rule ◽  
Intrusion Detection System ◽  
Association Rule Mining ◽  
Detection System ◽  
False Positive Rate ◽  
Rule Mining ◽  
Detection Rates ◽  
Mining Algorithm ◽  
Positive Rate

Intrusion detection system (IDSs) are important to industries and organizations to solve the problems of networks, and various classifiers are used to classify the activity as malicious or normal. Today, the security has become a decisive part of any industrial and organizational information system. This chapter demonstrates an association rule-mining algorithm for detecting various network intrusions. The KDD dataset is used for experimentation. There are three input features classified as basic features, content features, and traffic features. There are several attacks are present in the dataset which are classified into Denial of Service (DoS), Probe, Remote to Local (R2L), and User to Root (U2R). The proposed method gives significant improvement in the detection rates compared with other methods. Association rule mining algorithm is proposed to evaluate the KDD dataset and dynamic data to improve the efficiency, reduce the false positive rate (FPR) and provides less time for processing.

scholarly journals Identification and Clustering of Event Patterns From In Vivo Multiphoton Optical Recordings of Neuronal Ensembles

2008 ◽  
Vol 100 (1) ◽  
pp. 495-503 ◽  
Author(s):  
Ilker Ozden ◽  
H. Megan Lee ◽  
Megan R. Sullivan ◽  
Samuel S.-H. Wang
Keyword(s):  
Single Cells ◽  
False Positive Rate ◽  
Brain Structures ◽  
Data Sets ◽  
Cluster Number ◽  
Neuronal Ensembles ◽  
Neural Ensemble ◽  
Positive Rate ◽  
Indicator Dyes

In vivo multiphoton fluorescence microscopy allows imaging of cellular structures in brain tissue to depths of hundreds of micrometers and, when combined with the use of activity-dependent indicator dyes, opens the possibility of observing intact, functioning neural circuitry. We have developed tools for analyzing in vivo multiphoton data sets to identify responding structures and events in single cells as well as patterns of activity within the neural ensemble. Data were analyzed from populations of cerebellar Purkinje cell dendrites, which generate calcium-based complex action potentials. For image segmentation, active dendrites were identified using a correlation-based method to group covarying pixels. Firing events were extracted from dendritic fluorescence signals with a 95% detection rate and an 8% false-positive rate. Because an event that begins in one movie frame is sometimes not detected until the next frame, detection delays were compensated using a likelihood-based correction procedure. To identify groups of dendrites that tended to fire synchronously, a k-means-based procedure was developed to analyze pairwise correlations across the population. Because repeated runs of k-means often generated dissimilar clusterings, the runs were combined to determine a consensus cluster number and composition. This procedure, termed meta- k-means, gave clusterings as good as individual runs of k-means, was independent of random initial seeding, and allowed the exclusion of outliers. Our methods should be generally useful for analyzing multicellular activity recordings in a variety of brain structures.

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