scholarly journals Purification, identification, and characterization of two benzophenanthridine alkaloids from Corydalis longicalcarata rhizomes with anti-mitotic and polyploidy-inducing activities

2019 ◽  
Author(s):  
Jinhua Li ◽  
Ziqi Yan ◽  
Hongmei Li ◽  
Qiong Shi ◽  
Linfang Huang ◽  
...  
Keyword(s):  
Cell Division ◽  
Medicinal Plants ◽  
Crude Extract ◽  
Benzophenanthridine Alkaloids ◽  
Checkpoint Response ◽  
Antimitotic Activity ◽  
Chromosome Congression ◽  
Phenotypic Screen ◽  
Identification And Characterization

ABSTRACTMitosis has long been a therapeutic target for the treatment of cancer. After conducting a phenotypic screen for anti-mitotic activity in a crude extract library of more than 2000 medicinal plants, we identified the rhizomes of Corydalis longicalcarata. Guided by the bioactivity-based assay, two benzophenanthridine alkaloids were purified and subsequently identified as corynoline and its close analog acetylcorynoline. This study uncovers a previously unknown antimitotic activity for these two phytochemicals, discovers their potential to be developed as anticancer drugs, and highlights their pleiotropic effects on cell division, including prevention of chromosome congression, compromise of spindle checkpoint response, and blockage of cytokinesis.

scholarly journals Identification and characterization of phytoconstituents of ethanolic root extract of Clitoria ternatea L. utilizing HR-LCMS analysis

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
K N Jiji ◽  
P Muralidharan
Keyword(s):  
Nervous System ◽  
Medicinal Plants ◽  
The Body ◽  
Major Constituent ◽  
Root Extract ◽  
Active Metabolites ◽  
Clitoria Ternatea ◽  
Intellectual Capacity ◽  
Identification And Characterization

Medicinal plants act as a vital source in improving health and overcoming the side effects of modern-day medicine. Many evidence-based reports are present in the literature about the benefits of medicinal plants. Clitoria ternatea L. belongs to the family Fabaceae and is known to be one of the important Ayurvedic medicinal plant whose uses are specified mainly for the modification of nervous system activities. ‘Medhyarasayana’ is one of the Ayurvedic formulations which is used to promote the intellectual capacity, revive the body and nervous tissue, Clitoria ternatea serves as a major constituent of ‘Medhyarasayana.’ Identification and characterization of active metabolites of C. ternatea will help to isolate the important phytoconstituents responsible for the central nervous system effects, isolated components can be utilized in future for the formulation of new medicine for various neurodegenerative disorders. In the present study, the phytochemical evaluation of the ethanolic root extract of C. ternatea (EECT) was performed using the HR-LCMS technique. Preliminary qualitative phytoconstituents analysis showed the presence of tannins, alkaloids, saponins, steroids, carbohydrate, protein, flavonoids and triterpenoids in the ethanolic root extract. Almost 42 compounds were identified when the EECT subjected to HR-LCMS analysis.

Identification and characterization of ispA , a Shigella flexneri chromosomal gene essential for normal in vivo cell division and intercellular spreading

1996 ◽  
Vol 19 (3) ◽  
pp. 599-609 ◽  
Author(s):  
Ruairí A. Mac Síomóin ◽  
Noboru Nakata ◽  
Tatuo Murai ◽  
Masanosuke Yoshikawa ◽  
Hiroyuki Tsuji ◽  
...  
Keyword(s):  
Cell Division ◽  
Shigella Flexneri ◽  
Chromosomal Gene ◽  
Identification And Characterization

scholarly journals Identification and characterization of cell wall-cell division gene clusters in pathogenic gram-positive cocci.

1997 ◽  
Vol 179 (17) ◽  
pp. 5632-5635 ◽  
Author(s):  
M J Pucci ◽  
J A Thanassi ◽  
L F Discotto ◽  
R E Kessler ◽  
T J Dougherty
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Gene Clusters ◽  
Gram Positive ◽  
Wall Cell ◽  
Identification And Characterization ◽  
Gram Positive Cocci

scholarly journals Characterization of the Trans-Epithelial Transport of Green Tea (C. sinensis) Catechin Extracts with In Vitro Inhibitory Effect against the SARS-CoV-2 Papain-like Protease Activity

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6744
Author(s):  
Carmela Maria Montone ◽  
Sara Elsa Aita ◽  
Anna Arnoldi ◽  
Anna Laura Capriotti ◽  
Chiara Cavaliere ◽  
...  
Keyword(s):  
Green Tea ◽  
Crude Extract ◽  
Analytical Approach ◽  
Epithelial Transport ◽  
Synergistic Effects ◽  
Inhibitory Effect ◽  
Ic50 Value ◽  
Identification And Characterization

This work describes an untargeted analytical approach for the screening, identification, and characterization of the trans-epithelial transport of green tea (Camellia sinensis) catechin extracts with in vitro inhibitory effect against the SARS-CoV-2 papain-like protease (PLpro) activity. After specific catechin extraction, a chromatographic separation obtained six fractions were carried out. The fractions were assessed in vitro against the PLpro target. Fraction 5 showed the highest inhibitory activity against the SARS-CoV-2 PLpro (IC50 of 0.125 μg mL−1). The untargeted characterization revealed that (−)-epicatechin-3-gallate (ECG) was the most abundant compound in the fraction and the primary molecule absorbed by differentiated Caco-2 cells. Results indicated that fraction 5 was approximately 10 times more active than ECG (IC50 value equal to 11.62 ± 0.47 μg mL−1) to inhibit the PLpro target. Overall, our findings highlight the synergistic effects of the various components of the crude extract compared to isolated ECG.

scholarly journals Identification and characterization of the cell division protein MapZ from Streptococcus suis

2021 ◽  
Vol 10 (5) ◽  
Author(s):  
Muriel Dresen ◽  
Manfred Rohde ◽  
Jesús Arenas ◽  
Astrid Greeff ◽  
Andreas Nerlich ◽  
...  
Keyword(s):  
Cell Division ◽  
Streptococcus Suis ◽  
Cell Division Protein ◽  
Identification And Characterization

Phytochemical characterization of antimycobacterial crude extracts from medicinal plants traditionally used in Mozambique

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
X Luo ◽  
D Pires ◽  
JA Aínsa ◽  
B Gracia ◽  
S Mulhovo ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crude Extracts
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