scholarly journals The TARANI/ UBIQUITIN SPECIFIC PROTEASE 14 destabilizes the AUX/IAA transcriptional repressors and regulates auxin response in Arabidopsis thaliana

2019 ◽  
Author(s):  
Parinita Majumdar ◽  
Premananda Karidas ◽  
Imran Siddiqi ◽  
Utpal Nath
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Interaction ◽  
Leaf Shape ◽  
Protein Product ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Phenotypic Analysis ◽  
Molecular Control ◽  
Specific Protease ◽  
Turn Over

ABSTRACTAuxin response is regulated by a group of AUX/IAA transcriptional inhibitors that suppress auxin signaling in the absence of the hormone. While the degradation of these proteins upon auxin signaling has been well studied, the molecular control of their rapid turn-over is not clearly understood. Here, we report that the TARANI/ UBIQUITIN PROTEASE 14 protein in Arabidopsis thaliana (Arabidopsis) is required for AUX/IAA degradation. The tni mutation was originally identified in a forward genetic screen to isolate mutants with altered leaf shape. Detailed phenotypic analysis revealed that tni displays pleiotropic phenotypic alterations that resemble auxin-related defects. The activity of auxin responsive reporters DR5::GUS, DR5::nYFP and IAA2::GUS was reduced in tni organs, implying that TNI is required for normal auxin response. Genetic interaction studies suggested that TNI acts along with TIR1, ARF7, AUX1 and PIN1 – molecules involved in auxin signaling or transport. A map-based cloning approach combined with next-generation sequencing identified TNI as UBIQUITIN SPECIFIC PROTEASE14 which is involved in ubiquitin recycling. In tni, the mutant primary transcript is spliced inefficiently, which is predicted to produce an aberrant protein product in addition to the normal protein, where a polypeptide corresponding to the 3rd intron in inserted in-frame within the Zn-finger domain of UBP14. The tni plants accumulated poly-ubiquitin chains and excess poly-ubiquitinated proteins due to reduced TNI activity. Improper ubiquitin recycling affected the degradation of DII:VENUS, IAA18:GUS and HS::AXR3-NT:GUS, resulting in their stabilization in the tni mutant. Thus, our study identified a function for TNI/UBP14 in regulating auxin response through ubiquitin recycling.

Analysis of Drosophila proboscipedia mutant alleles

Genome ◽  
2004 ◽  
Vol 47 (3) ◽  
pp. 600-609 ◽  
Author(s):  
I Tayyab ◽  
H M Hallahan ◽  
A Percival-Smith
Keyword(s):  
Dna Sequence ◽  
Hox Genes ◽  
Genetic Interaction ◽  
Direct Interaction ◽  
Protein Product ◽  
Maxillary Palp ◽  
Phenotypic Analysis ◽  
Sex Combs Reduced ◽  
Important Region ◽  
Sex Combs

Proboscipedia (PB) is a HOX protein required for adult maxillary palp and proboscis formation. To identify domains of PB important for function, 21 pb point mutant alleles were sequenced. Twelve pb alleles had DNA sequence changes that encode an altered PB protein product. The DNA sequence changes of these 12 alleles fell into 2 categories: missense alleles that effect the PB homeodomain (HD), and nonsense or frameshift alleles that result in C-terminal truncations of the PB protein. The phenotypic analysis of the pb homeobox missense alleles suggests that the PB HD is required for maxillary palp and proboscis development and pb – Sex combs reduced (Scr) genetic interaction. The phenotypic analysis of the pb nonsense or frameshift alleles suggests that the C-terminus is an important region required for maxillary palp and proboscis development and pb–Scr genetic interaction. PB and SCR do not interact directly with one another in a co-immunoprecipitation assay and in a yeast two-hybrid analysis, which suggests the pb–Scr genetic interaction is not mediated by a direct interaction between PB and SCR.Key words: proboscipedia, Sex combs reduced, Hox genes, mutant analysis, Drosophila body plan, appendage development.

scholarly journals Uniform distribution of 35S promoter-driven mDII auxin control sensor in leaf primordia

2018 ◽  
Author(s):  
Chunmei Guan ◽  
Fei Du ◽  
Yuling Jiao
Keyword(s):  
Arabidopsis Thaliana ◽  
Uniform Distribution ◽  
Leaf Primordia ◽  
The Other ◽  
Auxin Signaling ◽  
35S Promoter ◽  
Light Penetration ◽  
Auxin Response ◽  
Whole Mount ◽  
Invaluable Tool

AbstractThe DII sensor has been an invaluable tool to map spatiotemporal auxin response and distribution in the model plant Arabidopsis thaliana. The DII sensor and mDII control sensor are driven by the widely used constitutive 35S promoter. However, the reliability of DII sensor has recently been questioned (Bhatia and Heisler, 2018). Here we provide additional evidence to show that the mDII control sensor is indeed uniformly distributed in early leaf primordia, which echoes the original reports (Vernoux et al., 2011; Brunoud et al., 2012). We also use DII/mDII and the PRS5A promoter-driven R2D2 sensors to confirm asymmetric auxin signaling in early leaf primordia. On the other hand, we provide evidence that light penetration may lead to artifacts during whole-mount imaging.

scholarly journals PRH1 mediates ARF7-LBD dependent auxin signaling to regulate lateral root development in Arabidopsis thaliana

2019 ◽  
Author(s):  
Feng Zhang ◽  
Wenqing Tao ◽  
Ruiqi Sun ◽  
Junxia Wang ◽  
Cuiling Li ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Transcription Factors ◽  
Root Development ◽  
Lateral Root ◽  
Lateral Roots ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Cell Identity ◽  
Lateral Root Development ◽  
Lateral Organ

AbstractThe development of lateral roots in Arabidopsis thaliana is strongly dependent on signaling directed by the AUXIN RESPONSE FACTOR7 (ARF7), which in turn activates LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors (LBD16, 18, 29 and 33). Here, the product of PRH1, a PR-1 homolog annotated previously as encoding a pathogen-responsive protein, was identified as a target of ARF7-mediated auxin signaling and also as participating in the development of lateral roots. PRH1 was shown to be strongly induced by auxin treatment, and plants lacking a functional copy of PRH1 formed fewer lateral roots. The transcription of PRH1 was controlled by the binding of both ARF7 and LBDs to its promoter region. An interaction was detected between PRH1 and GATA23, a protein which regulates cell identity in lateral root founder cells.Author SummaryIn Arabidopsis thaliana AUXIN RESPONSE FACTOR7 (ARF7)-mediated auxin signaling plays a key role in lateral roots (LRs) development. The LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors (LBD16, 18, 29 and 33) act downstream of ARF7-mediated auxin signaling to control LRs formation. Here, the PR-1 homolog PRH1 was identified as a novel target of both ARF7 and LBDs (especially the LBD29) during auxin induced LRs formation, as both ARF7 and LBDs were able to bind to the PRH1 promoter. More interestingly, PRH1 has a physical interaction with GATA23, which has been also reported to be up-regulated by auxin and influences LR formation through its regulation of LR founder cell identity. Whether the interaction between GATA23 and PRH1 affects the stability and/or the activity of either (or both) of these proteins remains an issue to be explored. This study provides improves new insights about how auxin regulates lateral root development.

scholarly journals Sound Waves Promote Arabidopsis thaliana Root Growth by Regulating Root Phytohormone Content

2021 ◽  
Vol 22 (11) ◽  
pp. 5739
Author(s):  
Joo Yeol Kim ◽  
Hyo-Jun Lee ◽  
Jin A Kim ◽  
Mi-Jeong Jeong
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Division ◽  
Root Growth ◽  
Apical Meristem ◽  
Cell Number ◽  
Root Apical Meristem ◽  
Control Group ◽  
Auxin Signaling ◽  
Ethylene Signaling ◽  
Sound Waves

Sound waves affect plants at the biochemical, physical, and genetic levels. However, the mechanisms by which plants respond to sound waves are largely unknown. Therefore, the aim of this study was to examine the effect of sound waves on Arabidopsis thaliana growth. The results of the study showed that Arabidopsis seeds exposed to sound waves (100 and 100 + 9k Hz) for 15 h per day for 3 day had significantly longer root growth than that in the control group. The root length and cell number in the root apical meristem were significantly affected by sound waves. Furthermore, genes involved in cell division were upregulated in seedlings exposed to sound waves. Root development was affected by the concentration and activity of some phytohormones, including cytokinin and auxin. Analysis of the expression levels of genes regulating cytokinin and auxin biosynthesis and signaling showed that cytokinin and ethylene signaling genes were downregulated, while auxin signaling and biosynthesis genes were upregulated in Arabidopsis exposed to sound waves. Additionally, the cytokinin and auxin concentrations of the roots of Arabidopsis plants increased and decreased, respectively, after exposure to sound waves. Our findings suggest that sound waves are potential agricultural tools for improving crop growth performance.

scholarly journals Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis

2021 ◽  
Vol 22 (1) ◽  
pp. 437
Author(s):  
Meng Wang ◽  
Panpan Li ◽  
Yao Ma ◽  
Xiang Nie ◽  
Markus Grebe ◽  
...  
Keyword(s):  
Root Elongation ◽  
Auxin Transport ◽  
Sterol Composition ◽  
Polar Auxin Transport ◽  
Sterol Biosynthesis ◽  
Auxin Biosynthesis ◽  
Root Tip ◽  
Auxin Signaling ◽  
Auxin Response ◽  
Short Root

Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation.

Overexpressed BRH1, a RING finger gene, alters rosette leaf shape in Arabidopsis thaliana

2017 ◽  
Vol 61 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Xiaoqian Wang ◽  
Eryong Chen ◽  
Xiaoyang Ge ◽  
Qian Gong ◽  
HamamaIslam Butt ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Leaf Shape ◽  
Ring Finger ◽  
Rosette Leaf

scholarly journals MicroRNAs Are Involved in Regulating Plant Development and Stress Response through Fine-Tuning of TIR1/AFB-Dependent Auxin Signaling

2022 ◽  
Vol 23 (1) ◽  
pp. 510
Author(s):  
Pan Luo ◽  
Dongwei Di ◽  
Lei Wu ◽  
Jiangwei Yang ◽  
Yufang Lu ◽  
...  
Keyword(s):  
Stress Response ◽  
Fine Tuning ◽  
Auxin Signaling ◽  
In Planta ◽  
Auxin Response ◽  
Signal Molecule ◽  
Master Regulators ◽  
Non Coding Rna ◽  
Complex Regulation ◽  
Indole 3 Acetic Acid

Auxin, primarily indole-3-acetic acid (IAA), is a versatile signal molecule that regulates many aspects of plant growth, development, and stress response. Recently, microRNAs (miRNAs), a type of short non-coding RNA, have emerged as master regulators of the auxin response pathways by affecting auxin homeostasis and perception in plants. The combination of these miRNAs and the autoregulation of the auxin signaling pathways, as well as the interaction with other hormones, creates a regulatory network that controls the level of auxin perception and signal transduction to maintain signaling homeostasis. In this review, we will detail the miRNAs involved in auxin signaling to illustrate its in planta complex regulation.

scholarly journals KNOX HOMOLOGS SHOOT MERISTEMLESS (STM) and KNAT6 are epistatic to CLAVATA3 (CLV3) during embryonic meristem development in Arabidopsis thaliana

2020 ◽  
Author(s):  
Sharma Nidhi ◽  
Liu Tie
Keyword(s):  
Arabidopsis Thaliana ◽  
Shoot Apex ◽  
Double Mutant ◽  
Genetic Interaction ◽  
Homeobox Gene ◽  
The Other ◽  
Shoot Meristem ◽  
Published Data ◽  
Meristem Development ◽  
Shoot Meristemless

AbstractIn Arabidopsis, the genes SHOOT MERISTEMLESS (STM) and CLAVATA3 (CLV3) antagonistically regulate shoot meristem development. STM is essential for both development and maintenance of the meristem, as stm mutants fail to develop a shoot meristem during embryogenesis. CLV3, on the other hand, negatively regulates meristem proliferation, and clv3 mutants possess an enlarged shoot meristem. Genetic interaction studies revealed that stm and clv3 dominantly suppress each other’s phenotypes. STM works in conjunction with its closely related homologue KNOTTED1-LIKE HOMEOBOX GENE 6 (KNAT6) to promote meristem development and organ separation, as stm knat6 double mutants fail to form a meristem and produce a fused cotyledon. In this study, we show that clv3 fails to promote post-embryonic meristem formation in stm-1 background if we also remove KNAT6. stm-1 knat6 clv3 triple mutants result in early meristem termination and produce fused cotyledons similar to stm knat6 double mutant. Notably, the stm-1 knat6 and stm-1 knat6 clv3 alleles lack tissue in the presumed region of SAM. stm knat6 clv3 also showed reduced inflorescence size and shoot apex size as compared to clv3 single or stm clv3 double mutants. In contrast to previously published data, these data suggest that stm is epistatic to clv3 in postembryonic meristem development.HighlightSTM and KNAT6 genes determine post-embryonic meristem formation and activity in Arabidopsis. clv3 mutation is unable to rescue the stm knat6 meristemless phenotype.

Alteration in flowering time causes accelerated or decelerated progression through Arabidopsis vegetative phases

2001 ◽  
Vol 79 (6) ◽  
pp. 657-665 ◽  
Author(s):  
Quintin J Steynen ◽  
Dee A Bolokoski ◽  
Elizabeth A Schultz
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Leaf Shape ◽  
Central Mechanism ◽  
Second Phase ◽  
Wild Type ◽  
Low Light ◽  
Terminal Flower ◽  
Vegetative Phases ◽  
Short Days

We have identified three phases within the wild-type Arabidopsis thaliana (L.) Heynh. rosette, based on significant differences in leaf shape, size, vascular pattern, and presence of abaxial trichomes. To test the hypothesis that a single, central mechanism controls the progression through all plant phases and that conditions that alter the time to flowering will also alter the progression through vegetative phases, we analysed the rosette phases under such conditions. In support of our hypothesis, we determined that those conditions (loss of LEAFY activity, short days) that decelerate time to flowering show decelerated progression through the rosette phases, while those conditions (loss of TERMINAL FLOWER, overexpression of LEAFY, low light) that accelerate time to flowering show accelerated progression through the rosette phases. In all conditions except short days, the length of the first phase was unaffected, indicating that this phase is less susceptible to influences of the central mechanism. Progression through the subsequent two rosette phases was accelerated differentially, such that the second phase was affected more strongly than the first. This supports the idea that, in the rosette, as in the inflorescence, the inhibition of phase transition by the central mechanism is gradually decreasing.Key words: phase change, flowering time, Arabidopsis thaliana, LEAFY, TERMINAL FLOWER, heteroblasty.

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