scholarly journals Developmental plasticity of Brachypodium distachyon in response to P deficiency: modulation by inoculation with phosphate-solubilizing bacteria

2019 ◽  
Author(s):  
Caroline Baudson ◽  
Benjamin M. Delory ◽  
Stijn Spaepen ◽  
Patrick du Jardin ◽  
Pierre Delaplace
Keyword(s):  
Root System ◽  
Developmental Plasticity ◽  
Brachypodium Distachyon ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Cultivation System ◽  
P Use Efficiency ◽  
Poorly Soluble ◽  
Use Efficiency ◽  
The Impact

AbstractBackgroundMineral P fertilisers must be used wisely in order to preserve rock phosphate, a limited and non-renewable resource. The use of bio-inoculants to improve soil nutrient availability and trigger an efficient plant response to nutrient deficiency is one potential strategy in the attempt to decrease P inputs in agriculture.MethodA gnotobiotic co-cultivation system was used to study the response of Brachypodium distachyon to contrasted P supplies (soluble and poorly soluble forms of P) and inoculation with P solubilizing bacteria. Brachypodium’s responses to P conditions and inoculation with bacteria were studied in terms of developmental plasticity and P use efficiency.ResultsBrachypodium showed plasticity in its biomass allocation pattern in response to variable P conditions, specifically by prioritizing root development over shoot productivity under poorly soluble P conditions. Despite the ability of the bacteria to solubilize P, shoot productivity was depressed in plants inoculated with bacteria, although the root system development was maintained. The negative impact of bacteria on biomass production in Brachypodium might be attributed to inadequate C supply to bacteria, an increased competition for P between both organisms under P-limiting conditions, or an accumulation of toxic bacterial metabolites in our cultivation system. Both P and inoculation treatments impacted root system morphology. The modulation of Brachypodium’s developmental response to P supplies by P solubilizing bacteria did not lead to improved P use efficiency.ConclusionOur results support the hypothesis that plastic responses of Brachypodium cultivated under P-limited conditions are modulated by P solubilizing bacteria. The considered experimental context impacts plant–bacteria interactions. Choosing experimental conditions as close as possible to real ones is important in the selection of P solubilizing bacteria. Both persistent homology and allometric analyses proved to be useful tools that should be considered when studying the impact of bio-inoculants on plant development in response to varying nutritional context.

scholarly journals Synergy between a shallow root system with a DRO1 homologue and localized P application improves P uptake of lowland rice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aung Zaw Oo ◽  
Yasuhiro Tsujimoto ◽  
Mana Mukai ◽  
Tomohiro Nishigaki ◽  
Toshiyuki Takai ◽  
...  
Keyword(s):  
Root System ◽  
Crop Production ◽  
Soil Surface ◽  
P Uptake ◽  
Ore Deposits ◽  
Root Surface Area ◽  
Genetic Traits ◽  
P Use Efficiency ◽  
Use Efficiency ◽  
P Application

AbstractImproved phosphorus (P) use efficiency for crop production is needed, given the depletion of phosphorus ore deposits, and increasing ecological concerns about its excessive use. Root system architecture (RSA) is important in efficiently capturing immobile P in soils, while agronomically, localized P application near the roots is a potential approach to address this issue. However, the interaction between genetic traits of RSA and localized P application has been little understood. Near-isogenic lines (NILs) and their parent of rice (qsor1-NIL, Dro1-NIL, and IR64, with shallow, deep, and intermediate root growth angles (RGA), respectively) were grown in flooded pots after placing P near the roots at transplanting (P-dipping). The experiment identified that the P-dipping created an available P hotspot at the plant base of the soil surface layer where the qsor1-NIL had the greatest root biomass and root surface area despite no genotyipic differences in total values, whereby the qsor1-NIL had significantly greater biomass and P uptake than the other genotypes in the P-dipping. The superior surface root development of qsor1-NIL could have facilitated P uptakes from the P hotspot, implying that P-use efficiency in crop production can be further increased by combining genetic traits of RSA and localized P application.

scholarly journals Synergy Between a Shallow Root System With a DRO1 Homologue and Localized P Application Improves Rice P Uptake 

2021 ◽  
Author(s):  
Aung Zaw Oo ◽  
YASUHIRO TSUJIMOTO ◽  
Mana Mukai ◽  
Tomohiro Nishigaki ◽  
Toshiyuki Takai ◽  
...  
Keyword(s):  
Root System ◽  
Crop Production ◽  
Soil Layer ◽  
P Uptake ◽  
Ore Deposits ◽  
Root Surface Area ◽  
Genetic Traits ◽  
P Use Efficiency ◽  
Use Efficiency ◽  
P Application

Abstract Improved phosphorus (P) use efficiency for crop production is needed given the depleting phosphorus ore deposits and increasing ecological concerns about its excessive use. Root system architecture (RSA) is important in efficiently capturing immobile P in soils, while agronomically, localized P application near the roots is a potential approach to address this issue. However, the interaction between genetic traits of RSA and localized P application has been little understood. Near-isogenic lines (NILs) and their parent of rice (qsor1-NIL, Dro1-NIL, and IR64, with shallow, deep, and intermediate root growth angles (RGA), respectively) were grown in flooded pots after placing P near the roots at transplanting (P-dipping). The experiment identified that the P-dipping created an available P hotspot at the soil surface; the qsor1-NIL had the greatest root biomass and root surface area in the 0–3 cm soil layer despite no genotype differences in total values; the qsor1-NIL had significantly greater biomass and P uptake than the other genotypes in the P-dipping. The superior surface root development of qsor1-NIL could have facilitated P uptakes from the P hotspot, implying that P-use efficiency in crop production can be further increased by combining genetic traits of RSA and localized P application.

scholarly journals Regulatory effect of phosphorus and nitrogen on nodulation and plant performance of leguminous shrubs

AoB Plants ◽  
2019 ◽  
Author(s):  
M A Míguez-Montero ◽  
A Valentine ◽  
M A Pérez-Fernández
Keyword(s):  
N Use Efficiency ◽  
P Availability ◽  
Physiological Basis ◽  
P Use Efficiency ◽  
Key Species ◽  
Use Efficiency ◽  
Inoculation Treatment ◽  
The Impact ◽  
P Supply ◽  
N Use

Abstract The impact of phosphorus (P) nutrition on plant growth, symbiotic N2 fixation, and phosphorus and nitrogen use and their assimilation was investigated in four leguminous plants of the genus Cytisus. Plants inoculated with Rhizobium strains isolated from plants of the four species growing in the wild were crop under controlled conditions in soils with either low P (5 µM) or high P (500 µM). The experiment was replicated in the presence and absence of plant irrigation to test for the effects of drought stress of inoculated and non-inoculated plants under the two P levels of fertilization. P-low treatments increased nodule production while plant biomass and shoot and root P and N contents were maximum at sufficient P. The reduction of P in the soil clearly induced biological nitrogen fixation and greater phosphorus and nitrogen uptake efficiencies, as shown by the total N and P accumulated in plants. Similarly, distinct tolerances to drought support this idea. Cytisus balansae had the lowest tolerance to water scarcity. Cytisus multiflorus and Cytisus scoparius were the most resistant species to drought, with this resistance enhanced in the inoculated plants. In the four species, the inoculation treatment clearly enhanced N-use efficiency, whereas P-use efficiency was greater in the non-inoculated plants in the irrigated treatment. With a P-induced demand for N, the plants nodulated prolifically and increased N supply from biological fixation. The physiological basis for N2-fixing C. scoparius and C. striatus maintaining growth at low P supply and responding to greater P supply is through balanced acquisition of P and N for plant demand. Native shrubby legumes are key species in natural ecosystems due to their capability to increase the net N budget in plants and soils and because they modulate the phosphorus availability. Four legumes in the genus Cytisus either native to or commonly represented in the Iberian Peninsula proved to be able shift their N use depending on the soil P availability and on the interactions established with their Bradyrhizobium symbionts. In the four species the inoculation treatment clearly enhanced N-use efficiency and drought tolerance, whereas P-use efficiency was greater in the non-inoculated plants in the irrigated treatment.

scholarly journals Synergy between a shallow root system with a DRO1 homologue and localized P application improves rice P uptake

2020 ◽  
Author(s):  
Aung Zaw Oo ◽  
Yasuhiro Tsujimoto ◽  
Mana Mukai ◽  
Tomohiro Nishigaki ◽  
Toshiyuki Takai ◽  
...  
Keyword(s):  
Root System ◽  
Crop Production ◽  
Management Practices ◽  
Soil Layer ◽  
P Uptake ◽  
The Other ◽  
Genetic Traits ◽  
P Use Efficiency ◽  
Use Efficiency ◽  
P Application

ABSTRACTThe development of genotypes and fertilizer management practices that facilitate high phosphorus (P) use efficiency is needed given the depleting phosphorus ore deposits and increasing ecological concerns about its excessive use. Root system architecture (RSA) is important in efficiently capturing immobile P in soils, while agronomically, localized P application near the roots is a potential approach to address this issue. However, the interaction between genetic traits of RSA and localized P application has not been examined. Near-isogenic lines (NILs) and their parent of rice (qsor1-NIL, Dro1-NIL, and IR64, with shallow, deep, and intermediate root growth angles (RGA), respectively) were grown in flooded pots in a uniform and P-sufficient condition (Pinco), and with localized P application by dipping seedling roots into P-enriched slurry at transplanting (P-dipping). The P-dipping created an available P hotspot at the soil surface and substantially improved applied P-use efficiency (equivalent biomass at one fifth of application rate of the Pinco). Further, the qsor1-NIL had significantly greater biomass and P uptake than the other genotypes in the P-dipping. The qsor1-NIL consistently had a greater root biomass and surface area in the 0–3 cm soil layer, despite that there were no genotype differences in total values and that the other genotypes also reduced their RGAs responding to the P hotspot in the P-dipping. The shallow root system of qsor1-NIL facilitated P uptake from the P hotspot. P-use efficiency in crop production can be further increased by combining genetic traits of RSA and localized P application.

scholarly journals Unravelling the phosphorus use efficiency associated traits in mungbean (Vigna radiata L.) under low phosphorus condition

2020 ◽  
Vol 80 (04) ◽  
Author(s):  
Harsh Kumar Dikshit ◽  
Venkata Ravi Prakash Reddy ◽  
Gyan Prakash Mishra ◽  
Muraleedhar Aski ◽  
Renu Pandey ◽  
...  
Keyword(s):  
Stepwise Regression ◽  
Chlorophyll Concentration ◽  
Dry Weight ◽  
Phosphorus Use Efficiency ◽  
Root Volume ◽  
P Deficiency ◽  
Mean Values ◽  
P Use Efficiency ◽  
Low Phosphorus ◽  
Use Efficiency

Phosphorus (P) deficiency is one of the serious problems affecting plant growth in mungbean in different parts of the world. The root, shoot and biomass related traits were investigated for identifying P-efficient genotypes in 54 mungbean genotypes under low-P (LP) and normal-P (NP) conditions. In this study, the membership function value of P use efficiency of studied traits was used as a compendious index for studying P use efficiency (PUE) in mungbean. Among the studied traits, mean values of total root volume, chlorophyll concentration, root dry weight (RDW) and root to shoot ratio increased >25% under LP condition indicating that these traits are highly responsive to P deficiency. Correlation and stepwise regression analysis revealed that RDW explained most of the variation and could be used as a clear indicator of PUE. The five highly P-efficient genotypes namely, MH 805, M 42, PUSA 9531, EC 398885 and M 209 with high MFVP values may be used for PUE improvement in mungbean.

scholarly journals Response of Wheat to Foliar and Soil P Fertilization on Grain Yield and Phosphorus use Efficiency in Southeastern Algeria

2020 ◽  
Author(s):  
N. Boukhalfa-Deraoui ◽  
L. Hanifi-Mekliche ◽  
A. Mekliche
Keyword(s):  
Grain Yield ◽  
Available P ◽  
Wheat Crop ◽  
Phosphorus Use Efficiency ◽  
P Deficiency ◽  
Soil P ◽  
P Use Efficiency ◽  
P Fertilizer ◽  
Use Efficiency ◽  
Triticum Durum Desf

Background: P deficiency is very common in alkaline - calcareous soil. Therefore, application of foliar-absorbed fertilizers may be an effective strategy to overcome the low bioavailability of phosphorus in soil, by improving phosphorus use efficiency and reduced nutrients loses.Methods: A field experiment was carried out in 2006-07 growing season at El-Menia (southeastern Algeria) to evaluated the effect of two foliar P (agriphos and leader-start) and three soil P (TSP P 46, Fosfactyl NP 3:22 and NPKs 8:36:13,5+15) on yield and P use efficiency of durum wheat crop Triticum durum Desf. var. Carioca and on available P and total P in soil.Result: Data showed that significant effect of soil P fertilizer on grain yield components (ears m-², grains ear-1 and the 1000 grains weight), grain P use efficiency and available P in soil. The best values were recorded by NPKs fertilizer, but no differences were observed for these parameters among foliar fertilizer sources.

Relationships of phosphorus concentration in reproductive organs with soil phosphorus availability for tropical rain-forest trees on Mount Kinabalu, Borneo

2018 ◽  
Vol 34 (6) ◽  
pp. 351-363
Author(s):  
Yuki Tsujii ◽  
Kanehiro Kitayama
Keyword(s):  
Reproductive Organs ◽  
Reproductive Organ ◽  
Phosphorus Concentration ◽  
P Availability ◽  
P Deficiency ◽  
P Use Efficiency ◽  
Tree Community ◽  
P Concentration ◽  
Mount Kinabalu ◽  
Use Efficiency

Abstract:Bornean rain forests on phosphorus (P)-poor soils exhibit a high P-use efficiency in the production of reproductive organs (i.e. the inverse of P concentration in reproductive-organ litter). The mechanism underpinning this high P-use efficiency is not known, but is hypothesized to result from dilution of P in a given type of reproductive organ and/or a shift of the community composition of flower/fruit types with decreasing P availability. These hypotheses were tested using eight forests with different soil P availabilities on Mount Kinabalu, Borneo. Mean P concentration per forest by genus in inflorescences was significantly positively correlated with P availability, while that in seeds or pericarps was not significantly correlated. This trend was consistent across 21 genera that we analysed, suggesting that P concentration in seeds is maintained in exchange with the dilution of P in inflorescences. The composition of fruit types in tree community was estimated based on the relative abundances of genera in each forest. The relative abundance of capsulate species, which required less P in pericarps, tended to increase in tree community with decreasing P availability. Therefore, both mechanisms were involved in P-use efficiency. This work provides an insight into the reproductive adaptation of trees to P deficiency.

scholarly journals Time-resolution of the shoot and root growth of the model cereal Brachypodium in response to inoculation with Azospirillum bacteria at low phosphorus and temperature

2020 ◽  
Author(s):  
Martino Schillaci ◽  
Borjana Arsova ◽  
Robert Walker ◽  
Penelope M. C. Smith ◽  
Kerstin A. Nagel ◽  
...  
Keyword(s):  
Root Growth ◽  
Molecular Mechanisms ◽  
Invasive Plant ◽  
Brachypodium Distachyon ◽  
Plant Phenotyping ◽  
Shoot Biomass ◽  
P Use Efficiency ◽  
P Concentration ◽  
Use Efficiency ◽  
Shoot And Root Growth

Abstract A non-invasive plant phenotyping platform, GrowScreen-PaGe, was used to resolve the dynamics of shoot and root growth of the model cereal Brachypodium (Brachypodium distachyon Bd21-3) in response to the plant growth promoting (PGP) bacteria Azospirillum (Azospirillum brasilense Sp245). Inoculated Brachypodium plants had greater early vigor and higher P use efficiency than non-inoculated Brachypodium at low P and low temperature conditions. Root systems were imaged non-invasively at eight time points and data combined with leaf area, shoot biomass and nutrient content from destructive subsamples at 7, 14 and 21 days after inoculation (DAI). Azospirillum colonisation of roots improved Brachypodium shoot and, to a greater degree, root growth in three independent experiments. Inoculation promoted P use efficiency in shoots but not P concentration or uptake, despite increased total root length. Longer roots in inoculated plants arose from twofold faster branch root growth but slower axile root growth, detected at 11 DAI. Analysis of the spatio-temporal phenotypes indicated that the effects of Azospirillum inoculation increased as shoot P concentration declined, but the magnitude depended on the time after inoculation and growth rate of branch roots compared to axile roots. High throughput plant phenotyping platforms allow the details of plant-microorganism symbioses to be resolved, offering insights into the timing of changes in different tissues to allow molecular mechanisms to be determined.

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