scholarly journals Patterning of Virus-Infected Glycine max Seed Coat Is Associated with Suppression of Endogenous Silencing of Chalcone Synthase Genes

2004 ◽  
Vol 16 (4) ◽  
pp. 807-818 ◽  
Author(s):  
Mineo Senda ◽  
Chikara Masuta ◽  
Shizen Ohnishi ◽  
Kazunori Goto ◽  
Atsushi Kasai ◽  
...  

2009 ◽  
Vol 59 (4) ◽  
pp. 419-426 ◽  
Author(s):  
Tasuku Kurauchi ◽  
Takuro Matsumoto ◽  
Akito Taneda ◽  
Teruo Sano ◽  
Mineo Senda


Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.





1991 ◽  
Vol 10 (9) ◽  
pp. 2605-2612 ◽  
Author(s):  
P. Franken ◽  
U. Niesbach-Klösgen ◽  
U. Weydemann ◽  
L. Maréchal-Drouard ◽  
H. Saedler ◽  
...  




2001 ◽  
Vol 91 (10) ◽  
pp. 941-947 ◽  
Author(s):  
Nina Fleysh ◽  
Deepali Deka ◽  
Maria Drath ◽  
Hilary Koprowski ◽  
Vidadi Yusibov

Infection of soybean (Glycine max) plants inoculated with particles of Alfalfa mosaic virus (AlMV) isolate 425 at 12 days after germination was monitored throughout the life cycle of the plant (vegetative growth, flowering, seed formation, and seed maturation) by western blot analysis of tissue samples. At 8 to 10 days after inoculation, the upper uninoculated leaves showed symptoms of virus infection and accumulation of viral coat protein (CP). Virus CP was detectable in leaves, stem, roots, seedpods, and seed coat up to 45 days postinoculation (dpi), but only in the seedpod and seed coat at 65 dpi. No virus accumulation was detected in embryos and cotyledons at any time during infection, and no seed transmission of virus was observed. Soybean plants inoculated with recombinant AlMV passaged from upper uninoculated leaves of infected plants showed accumulation of full-length chimeric AlMV CP containing rabies antigen in systemically infected leaves and seed coat. These results suggest the potential usefulness of plants and plant viruses as vehicles for producing proteins of biomedical importance in a safe and inexpensive manner. Moreover, even the soybean seed coat, treated as waste tissue during conventional processing for oil and other products, may be utilized for the expression of value-added proteins.



2019 ◽  
Vol 135 ◽  
pp. 1070-1081 ◽  
Author(s):  
Manoj Kumar ◽  
Anil Dahuja ◽  
Archana Sachdev ◽  
Charanjit Kaur ◽  
Eldho Varghese ◽  
...  


2002 ◽  
Vol 29 (12) ◽  
pp. 1500 ◽  
Author(s):  
Richard A. Jorgensen ◽  
Qiudeng Que ◽  
Carolyn A. Napoli

Transgenes designed to overexpress anthocyanin genes An6 (encoding dihydroflavonol-4-reductase) or Hf1 (encoding flavonoid-3′,5′-hydroxylase) in Petunia hybrida L. produced flower colour phenotypes similar to those caused by sense cosuppression of chalcone synthase (Chs) genes. However, unlike Chs, sense cosuppression of An6 and Hf1 resulted in female infertility in transgenotes exhibiting complete phenotypic suppression of anthocyanins. Female sterility appeared to be due to embryo abortion, with discolouration of ovules first appearing about 4 d post-fertilization, followed by gradual collapse of the ovule. Pollen from cosuppressed, female-sterile transgenotes placed on wild-type stigmas produced normal seed set, indicating that sterility of cosuppressed plants was maternally controlled. We suggest an hypothesis that cosuppression of An6 and Hf1 leads to accumulation of dihydroflavonols in the seed coat, a maternal tissue, and that this accumulation inhibits embryo growth, either directly or indirectly. In this hypothesis, direct inhibition of embryo growth would require that dihydroflavonols diffuse from the seed coat into the embryo and act there, whereas indirect inhibition would require that dihydroflavonols interfere with some capacity of the seed coat to promote embryo growth.



2013 ◽  
Vol 5 (3) ◽  
pp. 1065-1076 ◽  
Author(s):  
Kye Man Cho ◽  
Tae Joung Ha ◽  
Yong Bok Lee ◽  
Woo Duck Seo ◽  
Jun Young Kim ◽  
...  


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