Scanning texture analysis of lamellar bone using microbeam synchrotron X-ray radiation

2007 ◽  
Vol 40 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Wolfgang Wagermaier ◽  
Himadri S. Gupta ◽  
Aurélien Gourrier ◽  
Oskar Paris ◽  
Paul Roschger ◽  
...  
Keyword(s):  
Texture Analysis ◽  
Three Dimensional ◽  
Compact Bone ◽  
Axis Orientation ◽  
Thin Sections ◽  
X Ray ◽  
Synthetic Materials ◽  
Mineralized Tissues ◽  
Lamellar Texture ◽  
Dimensional Distribution

Texture analysis with microbeam scanning diffraction enables the local mapping of three-dimensional crystallite orientation in heterogeneous natural and synthetic materials. Cortical (compact) bone is an example of a hierarchically structured biocomposite, which is built mainly of cylindrical osteons, having a lamellar texture at the micrometre level. In this work, a combination of microbeam synchrotron X-ray texture analysis with thin sections of osteonal bone is used to measure the three-dimensional distribution of thec-axis orientation of the mineral apatite in bone with positional resolution of 1 µm. The data reduction procedure needed to go from the stereographic projection of X-ray intensity to the determination of the local orientation of mineralized collagen fibrils is described. The procedure can be applied to other mineralized tissues (such as trabecular bone and chitin) with micrometre scale and biologically controlled fibrillar texture.

Radiological anatomy of the oral cavity

2013 ◽  
Author(s):  
Martin E. Atkinson
Keyword(s):  
Soft Tissues ◽  
Three Dimensional ◽  
Compact Bone ◽  
X Rays ◽  
Radiological Anatomy ◽  
X Ray ◽  
Periodontal Tissues ◽  
Sensitive Film ◽  
Mineralized Tissues ◽  
Pass Through

The radiographs most frequently taken in general dental practice are of the teeth and their immidiate supporting tissues for detection of dental caries or assessment of bone loss in periodontal disease. Intraoral radiographs are taken by placing the X-ray-sensitive film or receptor in the mouth close to the teeth being investigated. Extraoral radiographs use larger films or receptors positioned externally and produce a view of the entire dentition and its supporting structures on a single film; they are used to ascertain the state of development of the dentitions prior to orthodontic treatment, for example. Dental panoramic tomographs (DPTs) are the most frequent extraoral radiographs. A radiograph is a negative photographic record. Dense structures such as bone are designated as radio-opaque; they absorb some X-rays and appear white on radiographs. More X-rays pass through less dense radiolucent structures such as air-filled cavities which show up as black areas. The contrast between different tissues of the structures which the X-ray beam passes through is determined by their radiodensity which, in turn, is largely due to their content of metallic elements. Calcium and iron are the prevalent heavy metals in the body. Calcium is combined with phosphate to form hydroxyapatite crystals in bones and mineralized tissues in teeth. Iron is present in haemoglobin in blood, but only large concentrations of blood, such as those found within the heart chambers, show up on X-rays. In sequence from densest to most lucent, the radiodensity of the dental and periodontal tissues are: enamel, dentine, cementum, compact bone, cancellous bone, demineralized carious enamel and dentine, dental soft tissues such as pulp and periodontal ligament, and air; gold and silver–mercury amalgam metallic restorative materials are even denser than enamel. A radiograph is a two-dimensional representation of a three-dimensional situation. The orientation of anatomical structures relative to the X-ray beam is a major factor determining their appearance on the film. For example, a beam travelling through the long axis of a radiodense structure will produce a whiter image on the film than one passing through its shorter axis because more X-rays are absorbed; the structure will also have a different shape.

3-D reconstruction of molecular shape from microcrystal thin sections

1983 ◽  
Vol 41 ◽  
pp. 748-749
Author(s):  
S. Cusack ◽  
J.-C. Jésior
Keyword(s):  
Three Dimensional ◽  
Molecular Shape ◽  
Biological Molecules ◽  
Fourier Space ◽  
Single Particles ◽  
Thin Sections ◽  
Standard Methods ◽  
X Ray ◽  
X Ray Crystallography ◽  
Dimensional Reconstruction

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).

scholarly journals 3D immersive visualization of micro-computed tomography and XRD texture datasets

2019 ◽  
Vol 34 (2) ◽  
pp. 97-102
Author(s):  
M. A. Rodriguez ◽  
T. T. Amon ◽  
J. J. M. Griego ◽  
H. Brown-Shaklee ◽  
N. Green
Keyword(s):  
Computed Tomography ◽  
Texture Analysis ◽  
Mechanical Strain ◽  
Three Dimensional ◽  
Multidimensional Analysis ◽  
Micro Computed Tomography ◽  
X Ray ◽  
3D Data ◽  
Pole Figures ◽  
Ct Data

Advancements in computer technology have enabled three-dimensional (3D) reconstruction, data-stitching, and manipulation of 3D data obtained on X-ray imaging systems such as micro-computed tomography (μ-CT). Likewise, intuitive evaluation of these 3D datasets can be enhanced by recent advances in virtual reality (VR) hardware and software. Additionally, the generation, viewing, and manipulation of 3D X-ray diffraction datasets, such as pole figures employed for texture analysis, can also benefit from these advanced visualization techniques. We present newly-developed protocols for porting 3D data (as TIFF-stacks) into a Unity gaming software platform so that data may be toured, manipulated, and evaluated within a more-intuitive VR environment through the use of game-like controls and 3D headsets. We demonstrate this capability by rendering μ-CT data of a polymer dogbone test bar at various stages of in situ mechanical strain. An additional experiment is presented showing 3D XRD data collected on an aluminum test block with vias. These 3D XRD data for texture analysis (χ, ϕ, 2θ dimensions) enables the viewer to visually inspect 3D pole figures and detect the presence or absence of in-plane residual macrostrain. These two examples serve to illustrate the benefits of this new methodology for multidimensional analysis.

scholarly journals Three-dimensional visualization of fossil flowers, fruits, seeds, and other plant remains using synchrotron radiation X-ray tomographic microscopy (SRXTM): new insights into Cretaceous plant diversity

2014 ◽  
Vol 88 (4) ◽  
pp. 684-701 ◽  
Author(s):  
Else Marie Friis ◽  
Federica Marone ◽  
Kaj Raunsgaard Pedersen ◽  
Peter R. Crane ◽  
Marco Stampanoni
Keyword(s):  
Synchrotron Radiation ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Fossil Plants ◽  
Post Processing ◽  
Thin Sections ◽  
X Ray ◽  
Rapid Acquisition ◽  
Fossil Material ◽  
Plant Fossil

The application of synchrotron radiation X-ray tomographic microscopy (SRXTM) to the study of mesofossils of Cretaceous age has created new possibilities for the three-dimensional visualization and analysis of the external and internal structure of critical plant fossil material. SRXTM provides cellular and subcellular resolution of comparable or higher quality to that obtained from permineralized material using thin sections or the peel technique. SRXTM also has the advantage of being non-destructive and results in the rapid acquisition of large quantities of data in digital form. SRXTM thus refocuses the effort of the investigator from physical preparation to the digital post-processing of X-ray tomographic data, which allows great flexibility in the reconstruction, visualization, and analysis of the internal and external structure of fossil material in multiple planes and in two or three dimensions. A review of recent applications in paleobotany demonstrates that SRXTM will dramatically expand the level of information available for diverse fossil plants. Future refinement of SRXTM approaches that further increases resolution and eases digital post-processing, will transform the study of mesofossils and create new possibilities for advancing paleobotanical knowledge. We illustrate these points using a variety of Cretaceous mesofossils, highlighting in particular those cases where SRXTM has been essential for resolving critical structural details that have enhanced systematic understanding and improved phylogenetic interpretations.

High Resolution Synchrotron X-Ray Diffraction Tomography Of Large-Grained Samples

1996 ◽  
Vol 437 ◽  
Author(s):  
D.P. Piotrowski ◽  
S.R. Stock ◽  
A. Guvenilir ◽  
J.D. Haase ◽  
Z.U. Rek
Keyword(s):  
Spatial Distribution ◽  
High Resolution ◽  
Three Dimensional ◽  
Polycrystalline Materials ◽  
Diffraction Tomography ◽  
Two Dimensional ◽  
X Ray Diffraction ◽  
Image Storage ◽  
X Ray ◽  
Dimensional Distribution

AbstractIn order to understand the macroscopic response of polycrystalline structural materials to loading, it is frequently essential to know the spatial distribution of strain as well as the variation of micro-texture on the scale of 100 μm. The methods must be nondestructive, however, if the three-dimensional evolution of strain is to be studied. This paper describes an approach to high resolution synchrotron x-ray diffraction tomography of polycrystalline materials. Results from model samples of randomly-packed, millimeter-sized pieces of Si wafers and of similarly sized single-crystal Al blocks have been obtained which indicate that polychromatic beams collimated to 30 μm diameter can be used to determine the depth of diffracting volume elements within ± 70 μm. The variation in the two-dimensional distribution of diffracted intensity with changing sample to detector separation is recorded on image storage plates and used to infer the depth of diffracting volume elements.

Three-Dimensional Coherent X-Ray Diffraction Microscopy

MRS Bulletin ◽  
2004 ◽  
Vol 29 (3) ◽  
pp. 177-181 ◽  
Author(s):  
Ian K. Robinson ◽  
Jianwei Miao
Keyword(s):  
Electron Microscopy ◽  
Three Dimensional ◽  
Real Space ◽  
Objective Lens ◽  
X Rays ◽  
X Ray Diffraction ◽  
Space Images ◽  
Thin Sections ◽  
X Ray ◽  
Penetration Ability

AbstractX-rays have been widely used in the structural analysis of materials because of their significant penetration ability, at least on the length scale of the granularity of most materials. This allows, in principle, for fully three-dimensional characterization of the bulk properties of a material. One of the main advantages of x-ray diffraction over electron microscopy is that destructive sample preparation to create thin sections is often avoidable. A major disadvantage of x-ray diffraction with respect to electron microscopy is its inability to produce real-space images of the materials under investigation—there are simply no suitable lenses available. There has been significant progress in x-ray microscopy associated with the development of lenses, usually based on zone plates, Kirkpatrick–Baez mirrors, or compound refractive lenses. These technologies are far behind the development of electron optics, particularly for the large magnification ratios needed to attain high resolution. In this article, the authors report progress toward the development of an alternative general approach to imaging, the direct inversion of diffraction patterns by computation methods. By avoiding the use of an objective lens altogether, the technique is free from aberrations that limit the resolution, and it can be highly efficient with respect to radiation damage of the samples. It can take full advantage of the three-dimensional capability that comes from the x-ray penetration. The inversion step employs computational methods based on oversampling to obtain a general solution of the diffraction phase problem.

The optical determination of preferred orientation in clays and its application to the study of microstructure in consolidated kaolin. І

1967 ◽  
Vol 300 (1461) ◽  
pp. 218-234 ◽  
Keyword(s):  
Three Dimensional ◽  
Optical Microscope ◽  
Preferred Orientation ◽  
Distribution Functions ◽  
Thin Sections ◽  
Clay Particles ◽  
Degree Of Orientation ◽  
Dimensional Distribution ◽  
Optical Determination

Although single clay particles can seldom be seen with an optical microscope the preferred orientation of an aggregate of clay particles may be investigated by studying in thin section the birefringence of the aggregate. The techniques of preparing thin sections of clay with a minimum of disturbance of the structure and for measuring the birefringence under crossed nicols are described. It is shown that the birefringence of the aggregate arises solely from the birefringence of the constituent particles at least for the porosities of the clay used in this study. If a model distribution function for the spatial orientation of the particles be adopted the birefringent behaviour of the aggregate may be predicted and birefringence observations may be used to interpret the degree of orientation of an aggregate in terms of a model parameter. Two- and three-dimensional distribution functions are considered and the two-dimensional theory is judged to be preferable.

scholarly journals Statistical Assessment of the Microstructure of Barite Aggregate from Different Deposits Using X-Ray Microtomography and Optical Microscopy

2017 ◽  
Vol 62 (2) ◽  
pp. 697-702
Author(s):  
N. Żołek ◽  
Z. Ranachowski ◽  
P. Ranachowski ◽  
D. Jóźwiak-Niedźwiedzka ◽  
S. Kudela ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Barium Sulfate ◽  
Three Dimensional ◽  
Empty Space ◽  
Paper Analysis ◽  
Micro Ct ◽  
Statistical Assessment ◽  
Thin Sections ◽  
X Ray ◽  
Additional Information

AbstractTwo different barite ore (barium sulfate BaSO4) specimens from different localizations were tested and described in this paper. Analysis of the microstructure was performed on polished sections, and on thin sections using X-ray microtomography (micro-CT), and optical microscopy (MO). Microtomography allowed obtaining three-dimensional images of the barite aggregate specimens. In the tomograms, the spatial distribution of the other polluting phases, empty space as well as cracks, pores, and voids – that exceeded ten micrometers of diameter-were possible to visualize. Also, the micro-CT allowed distinguishing between minerals of different density, like SiO2and BaSO4. Images obtained and analyzed on thin sections with various methods using the optical microscopy in transmitted light delivered additional information on the aggregate microstructure, i.e. allow for estimation of the different kinds of inclusions (like the different density of the minerals) in the investigated specimens. Above methods, which were used in the tests, completed each another in order to supply a set of information on inclusions’ distribution and to present the important differences of the barite aggregate specimens microstructure.

2D Magnetic Texture Analysis of Co–Cu Films

2017 ◽  
Vol 72 (5) ◽  
pp. 449-455 ◽  
Author(s):  
Mehmet Bayirli ◽  
Oznur Karaagac ◽  
Hakan Kockar ◽  
Mursel Alper
Keyword(s):  
Fourier Series ◽  
Texture Analysis ◽  
Easy Axis ◽  
Hysteresis Loops ◽  
X Ray Diffraction ◽  
Axis Orientation ◽  
Magnetic Texture ◽  
X Ray ◽  
Cu Films ◽  
Magnetic Easy Axis

AbstractThe magnetic textures for the produced magnetic materials are important concepts in accordance with technical applications. Therefore, the aim of this article is to determine 2D magnetic textures of electrodeposited Co–Cu films by the measurement of hysteresis loops at the incremented angles. For that, Co–Cu films were deposited with different Co2+ in the electrolyte. In addition, the easy-axis orientation in the films from the squareness values of the angles, Mp(β) obtained by the hysteresis loops have been numerically studied using the Fourier series analysis. The differences observed in the magnetic easy-axis distributions were attributed to changes of the incorporation of Co in the films with the change of Co2+ in the electrolyte. The coefficients of Fourier series (A0 and A2n ) were also computed for 2D films. It is seen that a systematic and small decrease in A0 and an obvious decrease in A2n (n=1) were observed with increasing incorporated Co in the films. Results imply that interactions cause slightly demagnetization effect accordance with higher incorporation of Co in the films. Furthermore, the crystal structure of the Co–Cu films analysed by X-ray diffraction revealed that the films have dominantly face-centred cubic structure. Film contents analysed by energy-dispersive X-ray spectroscopy and film morphologies observed by scanning electron microscope also support the magnetic texture analysis results found by numerical computation.

scholarly journals Analytic determination of the three-dimensional distribution of dislocations using synchrotron X-ray topography

2006 ◽  
Vol 39 (1) ◽  
pp. 106-108 ◽  
Author(s):  
J. M. Yi ◽  
Y. S. Chu ◽  
T. S. Argunova ◽  
J. H. Je
Keyword(s):  
Three Dimensional ◽  
Analytic Formula ◽  
Detector Plane ◽  
X Ray ◽  
Dimensional Distribution ◽  
Distribution Of Dislocations ◽  
Symmetric Reflection ◽  
Si Wafer

A technique, using a symmetric reflectionviaazimuthal rotation of a sample, is presented for characterization of the three-dimensional distribution of dislocations in single crystals. An analytic formula is derived to transform the three-dimensional geometry of a straight dislocation into its two-dimensional projection onto the detector plane. By fitting topographs to the formula, the orientations and locations of dislocations are quantitatively determined. The dislocations in a thermally stressed Si wafer are examined as an example.

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