Crystallization and preliminary diffraction analysis of the HincII restriction endonuclease–DNA complex
1999 ◽
Vol 55
(11)
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pp. 1943-1945
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Keyword(s):
Data Set
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Crystals of the 60 kDa dimeric HincII restriction enzyme bound to a 12 base-pair dyad-symmetric duplex DNA carrying the specific 5′-GTCGAC recognition site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as precipitating agent. The rod-shaped crystals belong to space group I222 (or I212121), with unit-cell dimensions a = 66.9, b = 176.7, c = 256.0 Å. There are most likely to be two dimeric complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.5 Å at 100 K, with an R merge of 4.8%.
1999 ◽
Vol 55
(7)
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pp. 1353-1355
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Crystallization and preliminary X-ray analysis of the 6-phospho-α-glucosidase from Bacillus subtilis
1999 ◽
Vol 55
(6)
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pp. 1212-1214
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1999 ◽
Vol 55
(11)
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pp. 1903-1905
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1998 ◽
Vol 54
(1)
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pp. 90-92
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