scholarly journals Crystallization and preliminary X-ray diffraction analysis of the periplasmic domain of theEscherichia coliaspartate receptor Tar and its complex with aspartate

2014 ◽  
Vol 70 (9) ◽  
pp. 1219-1223 ◽  
Author(s):  
Takeshi Mise ◽  
Hideyuki Matsunami ◽  
Fadel A. Samatey ◽  
Ichiro N. Maruyama
Keyword(s):  
Escherichia Coli ◽  
Sodium Chloride ◽  
Molecular Mechanisms ◽  
Bacterial Chemotaxis ◽  
Cell Surface Receptor ◽  
X Ray Diffraction ◽  
X Ray ◽  
Space Groups ◽  
Surface Receptor ◽  
Periplasmic Domain

The cell-surface receptor Tar mediates bacterial chemotaxis toward an attractant, aspartate (Asp), and away from a repellent, Ni2+. To understand the molecular mechanisms underlying the induction of Tar activity by its ligands, theEscherichia coliTar periplasmic domain with and without bound aspartate (Asp-Tar and apo-Tar, respectively) were each crystallized in two different forms. Using ammonium sulfate as a precipitant, crystals of apo-Tar1 and Asp-Tar1 were grown and diffracted to resolutions of 2.10 and 2.40 Å, respectively. Alternatively, using sodium chloride as a precipitant, crystals of apo-Tar2 and Asp-Tar2 were grown and diffracted to resolutions of 1.95 and 1.58 Å, respectively. Crystals of apo-Tar1 and Asp-Tar1 adopted space groupP41212, while those of apo-Tar2 and Asp-Tar2 adopted space groupsP212121andC2, respectively.

scholarly journals Crystallization and X-ray diffraction analysis of SpaE, a basal pilus protein from the gut-adaptedLactobacillus rhamnosusGG

2017 ◽  
Vol 73 (6) ◽  
pp. 321-327 ◽  
Author(s):  
Arjun K. Mishra ◽  
Abhin Kumar Megta ◽  
Airi Palva ◽  
Ingemar von Ossowski ◽  
Vengadesan Krishnan
Keyword(s):  
Escherichia Coli ◽  
Cell Wall ◽  
Molecular Mechanisms ◽  
Recombinant Expression ◽  
Lactobacillus Rhamnosus ◽  
X Ray Diffraction ◽  
X Ray ◽  
Pilin Subunit ◽  
Sad Phasing

SpaE is the predicted basal pilin subunit in the sortase-dependent SpaFED pilus from the gut-adapted and commensalLactobacillus rhamnosusGG. Thus far, structural characterization of the cell-wall-anchoring basal pilins has remained difficult and has been limited to only a few examples from pathogenic genera and species. To gain a further structural understanding of the molecular mechanisms that are involved in the anchoring and assembly of sortase-dependent pili in less harmful bacteria,L. rhamnosusGG SpaE for crystallization was produced by recombinant expression inEscherichia coli. Although several attempts to crystallize the SpaE protein were unsuccessful, trigonal crystals that diffracted to a resolution of 3.1 Å were eventually produced using PEG 3350 as a precipitant and high protein concentrations. Further optimization with a combination of additives led to the generation of SpaE crystals in an orthorhombic form that diffracted to a higher resolution of 1.5 Å. To expedite structure determination by SAD phasing, selenium-substituted (orthorhombic) SpaE crystals were grown and X-ray diffraction data were collected to 1.8 Å resolution.

Microstructure and crystal symmetry of Pb2Sr2(Y/Ca)Cu3O9−δ

1990 ◽  
Vol 48 (4) ◽  
pp. 64-65
Author(s):  
Y. P. Lin ◽  
J. S. Xue ◽  
J. E. Greedan
Keyword(s):  
Electron Diffraction ◽  
High Temperature Superconductors ◽  
Carbon Film ◽  
Basic Structure ◽  
Crystal Symmetry ◽  
X Ray Diffraction ◽  
X Ray ◽  
Space Groups ◽  
New Family ◽  
Convergent Beam

A new family of high temperature superconductors based on Pb2Sr2YCu3O9−δ has recently been reported. One method of improving Tc has been to replace Y partially with Ca. Although the basic structure of this type of superconductors is known, the detailed structure is still unclear, and various space groups has been proposed. In our work, crystals of Pb2Sr2YCu3O9−δ with dimensions up to 1 × 1 × 0.25.mm and with Tc of 84 K have been grown and their superconducting properties described. The defects and crystal symmetry have been investigated using electron microscopy performed on crushed crystals supported on a holey carbon film.Electron diffraction confirmed x-ray diffraction results which showed that the crystals are primitive orthorhombic with a=0.5383, b=0.5423 and c=1.5765 nm. Convergent Beam Electron Diffraction (CBED) patterns for the and axes are shown in Figs. 1 and 2 respectively.

Crystallization and preliminary X-ray diffraction studies of 3-methyladenine—DNA glycosylase II from Escherichia coli

1988 ◽  
Vol 204 (4) ◽  
pp. 1055-1056 ◽  
Author(s):  
Yuriko Yamagata ◽  
Kyoko Odawara ◽  
Ken-Ichi Tomita ◽  
Yusaku Nakabeppu ◽  
Mutsuo Sekiguchi
Keyword(s):  
Escherichia Coli ◽  
Dna Glycosylase ◽  
X Ray Diffraction ◽  
X Ray

The Structure and Antibacterial Activity of Taurine and its Magnesium Complex

2011 ◽  
Vol 366 ◽  
pp. 404-407
Author(s):  
Li Hua Wang
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Single Crystal ◽  
Dilution Method ◽  
Monoclinic Space Group ◽  
X Ray Diffraction ◽  
X Ray ◽  
Antibacterial Assay ◽  
Serial Dilution Method

The block single-crystals of taurine were obtained, and its structure was determined by single-crystal X-ray diffraction. The single-crystal X-ray analysis of taurine reveals that the crystal belongs monoclinic, space group P2(1)/c with a = 0.52824(10) nm, b = 1.1647(8) nm, c = 0.79236(13) nm, ß = 94.0850(10). The magnesium complex with taurine has been synthesized in ethanol. The antibacterial assay of the Mg (II) complex was measureed using a modified version of the 2-fold serial dilution method. The results show that the complex shows considerable antibacterial activity against escherichia coli, bacillus subtilis and staphylococcus white.

scholarly journals Evolutionary Conservation of Reactions in Translation

2002 ◽  
Vol 66 (3) ◽  
pp. 460-485 ◽  
Author(s):  
M. Clelia Ganoza ◽  
Michael C. Kiel ◽  
Hiroyuki Aoki
Keyword(s):  
Molecular Mechanisms ◽  
Tertiary Structure ◽  
Evolutionary Conservation ◽  
Initiation Factor ◽  
Structural Studies ◽  
X Ray Diffraction ◽  
X Ray ◽  
Common Features ◽  
New Information ◽  
And Function

SUMMARY Current X-ray diffraction and cryoelectron microscopic data of ribosomes of eubacteria have shed considerable light on the molecular mechanisms of translation. Structural studies of the protein factors that activate ribosomes also point to many common features in the primary sequence and tertiary structure of these proteins. The reconstitution of the complex apparatus of translation has also revealed new information important to the mechanisms. Surprisingly, the latter approach has uncovered a number of proteins whose sequence and/or structure and function are conserved in all cells, indicating that the mechanisms are indeed conserved. The possible mechanisms of a new initiation factor and two elongation factors are discussed in this context.

scholarly journals Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

2021 ◽  
Author(s):  
Davood Gheidari ◽  
Morteza Mehrdad ◽  
Saloomeh Maleki ◽  
Samanesadat Hosseini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Limited Area ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inhibition Concentration ◽  
Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

scholarly journals Copper(II) and Zinc(II) Complexes Derived from N,N’-Bis(4-bromosalicylidene)propane-1,3-diamine: Syntheses, Crystal Structures and Antimicrobial Activity

2021 ◽  
Vol 68 (1) ◽  
pp. 102-108
Author(s):  
Yu-Mei Hao
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Parapsilosis ◽  
Antibacterial Activities ◽  
X Ray Diffraction ◽  
Deprotonated Form ◽  
X Ray ◽  
Square Planar ◽  
Vis Spectroscopy ◽  
Elemental Analyses

A mononuclear copper(II) complex, [CuL] (1), and a phenolato-bridged trinuclear zinc(II) complex, [Zn3Cl2L2(DMF)2] (2), where L is the deprotonated form of N,N’-bis(4-bromosalicylidene)propane-1,3-diamine (H2L), have been prepared and characterized by elemental analyses, IR and UV-Vis spectroscopy, and single crystal X-ray diffraction. The Cu atom in complex 1 is in square planar coordination, while the terminal and central Zn atoms in complex 2 are in square pyramidal and octahedral coordination, respectively. The antibacterial activities of the complexes have been tested on the bacteria Staphylococcus aureus and Escherichia coli, and the yeast Candida parapsilosis.

Sign in / Sign up

Export Citation Format

Share Document