Raman Spectroscopy for the Assessment of the Spongy Bone Mineral Component after Flow Delipidation

2021 ◽  
Author(s):  
Elena Timchenko ◽  
Pavel Timchenko ◽  
Oleg Frolov ◽  
Elena Pisareva ◽  
Anastasia Radion ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Mineral Component ◽  
Spongy Bone

scholarly journals Calcitonin Gene-Related Peptide Influences Bone-Tendon Interface Healing Through Osteogenesis: Investigation in a Rabbit Partial Patellectomy Model

2021 ◽  
Vol 9 (7) ◽  
pp. 232596712110039
Author(s):  
Huabin Chen ◽  
Hongbin Lu ◽  
Jianjun Huang ◽  
Zhanwen Wang ◽  
Yang Chen ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Calcitonin Gene Related Peptide ◽  
Control Group ◽  
Mineral Density ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Partial Patellectomy ◽  
Antagonist Group ◽  
Cgrp Antagonist

Background: Calcitonin gene-related peptide (CGRP), which has been shown to play an important role in osteogenesis during fracture repair, is also widely distributed throughout the tendon and ligament. Few studies have focused on the role of CGRP in repair of the bone-tendon interface (BTI). Purpose: To explore the effect of CGRP expression on BTI healing in a rabbit partial patellectomy model. Study Design: Controlled laboratory study. Methods: A total of 60 mature rabbits were subjected to a partial patellectomy and then randomly assigned to CGRP, CGRP-antagonist, and control groups. In the CGRP-antagonist group, the CGRP receptor antagonist BIBN4096BS was administered to block CGRP receptors. The patella–patellar tendon complex was harvested at 8 and 16 weeks postoperatively and subjected to radiographic, microlaser Raman spectroscopy, histologic, and biomechanical evaluation. Results: Radiographic data showed that local CGRP expression improved the growth parameters of newly formed bone, including area and volumetric bone mineral density ( P < .05 for both). Raman spectroscopy revealed that the relative bone mineral composition increased in the CGRP group compared with in the control group and the CGRP-antagonist group ( P < .05 for both). Histologic testing revealed that the CGRP group demonstrated better integration, characterized by well-developed trabecular bone expansion from the residual patella and marrow cavity formation, at the 8- and 16-week time points. Mechanical testing demonstrated that the failure load, ultimate strength, and stiffness in the CGRP group were significantly higher than those in the control group ( P < .05 for all), whereas these parameters in the CGRP-antagonist group were significantly lower compared with those in the control group at 16 weeks after surgery ( P < .05 for all). Conclusion: Increasing the local concentration of CGRP in the early stages of BTI healing enhanced osteogenesis in a rabbit partial patellectomy model and promoted healing of the BTI injury, whereas treatment using a CGRP antagonist had the opposite effect. However, exogenous CGRP expression did not induce novel bone remolding. Clinical Relevance: CGRP may have potential as a new therapy for BTI injuries or may be added to postoperative regimens to facilitate healing.

scholarly journals Prostate cancer metastases alter bone mineral and matrix composition independent of effects on bone architecture in mice — A quantitative study using microCT and Raman spectroscopy

Bone ◽  
2013 ◽  
Vol 56 (2) ◽  
pp. 454-460 ◽  
Author(s):  
Xiaohong Bi ◽  
Julie A. Sterling ◽  
Alyssa R. Merkel ◽  
Daniel S. Perrien ◽  
Jeffry S. Nyman ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Quantitative Study ◽  
Bone Architecture ◽  
Matrix Composition ◽  
Cancer Metastases

Raman Spectroscopy of Bone Mineral Crystallization in a Microfluidic Chip

2010 ◽  
Author(s):  
Mary M. J. Tecklenburg ◽  
Brandon Whitman ◽  
P. M. Champion ◽  
L. D. Ziegler
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Microfluidic Chip

Validated Approaches for Quantification of Bone Mineral Crystallinity Using Transmission Fourier Transform Infrared (FT-IR), Attenuated Total Reflection (ATR) FT-IR, and Raman Spectroscopy

2018 ◽  
Vol 72 (11) ◽  
pp. 1581-1593 ◽  
Author(s):  
William Querido ◽  
Ramyasri Ailavajhala ◽  
Mugdha Padalkar ◽  
Nancy Pleshko
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Ir Spectra ◽  
Vibrational Spectroscopy ◽  
Bone Mineral ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Ft Ir ◽  
Mineral Crystallinity ◽  
Ir And Raman

Bone mineral crystallinity is an important factor determining bone quality and strength. The gold standard method to quantify crystallinity is X-ray diffraction (XRD), but vibrational spectroscopic methods present powerful alternatives to evaluate a greater variety of sample types. We describe original approaches by which transmission Fourier transform infrared (FT-IR), attenuated total reflection (ATR) FT-IR, and Raman spectroscopy can be confidently used to quantify bone mineral crystallinity. We analyzed a range of biological and synthetic apatite nanocrystals (10–25 nm) and found strong correlations between different spectral factors and the XRD determination of crystallinity. We highlight striking differences between FT-IR spectra obtained by transmission and ATR. In particular, we show for the first time the absence of the 1030 cm−1 crystalline apatite peak in ATR FT-IR spectra, which excludes its use for analyzing crystallinity using the traditional 1030/1020 cm−1 ratio. The ν4PO4 splitting ratio was also not adequate to evaluate crystallinity using ATR FT-IR. However, we established original approaches by which ATR FT-IR can be used to determine apatite crystallinity, such as the 1095/1115 and 960/1115 cm−1 peak ratios in the second derivative spectra. Moreover, we found a simple unified approach that can be applied for all three vibrational spectroscopy modalities: evaluation of the ν1PO4 peak position. Our results allow the recommendation of the most reliable analytical methods to estimate bone mineral crystallinity by vibrational spectroscopy, which can be readily implemented in many biomineralization, archeological and orthopedic studies. In particular, we present a step forward in advancing the use of the increasingly utilized ATR FT-IR modality for mineral research.

Effect of Hydrogen Peroxide Bleaching on Bone Mineral/Matrix Ratio

2002 ◽  
Vol 56 (8) ◽  
pp. 1035-1037 ◽  
Author(s):  
T.-C. Chen ◽  
D. A. Shea ◽  
M. D. Morris
Keyword(s):  
Hydrogen Peroxide ◽  
Raman Spectroscopy ◽  
Raman Spectrum ◽  
Bone Mineral ◽  
Peroxide Bleaching ◽  
Spectral Shifts ◽  
The Matrix ◽  
Mineral Matrix

It is shown that bone fluorescence reduction by bleaching in 30% hydrogen peroxide causes a change in the mineral/matrix ratio as measured by Raman spectroscopy. However, there are no observable spectral shifts in the mineral Raman spectrum or in the matrix Raman spectrum. Possible sources of the change in ratio are discussed. Peroxide bleaching is not recommended for fluorescence reduction in quantitative Raman spectroscopy.

scholarly journals Analysis of the mineral component for cortical bone tissue by Raman spectroscopy after ovariectomy and its treatment with allogeneic hydroxyapatite

2019 ◽  
Vol 1400 ◽  
pp. 066026
Author(s):  
E Timchenko ◽  
P Timchenko ◽  
E Pisareva ◽  
M Vlasov ◽  
L Volova ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cortical Bone ◽  
Bone Tissue ◽  
Mineral Component

Correlating chemical changes in subchondral bone mineral due to aging or defective type II collagen by Raman spectroscopy

2007 ◽  
Author(s):  
Karen A. Dehring ◽  
Blake J. Roessler ◽  
Michael D. Morris
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Subchondral Bone ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Chemical Changes

RAMAN SPECTROSCOPY REVEALS PHYLOGENETIC AND PHYSIOLOGICAL SIGNALS FOR CARBONATE CONTENT IN BONE MINERAL

2016 ◽  
Author(s):  
Holger Petermann ◽  
◽  
H. Catherine W. Skinner ◽  
Matteo Fabbri ◽  
Brian Ranger
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Physiological Signals ◽  
Carbonate Content

scholarly journals Characterization of Synthetic Hydroxyapatite Fibers Using High-Resolution, Polarized Raman Spectroscopy

2020 ◽  
pp. 000370282094254 ◽  
Author(s):  
Furqan A. Shah
Keyword(s):  
Raman Spectroscopy ◽  
High Resolution ◽  
Bone Mineral ◽  
Area Fraction ◽  
Accurate Estimation ◽  
Bovine Bone ◽  
Carbonated Apatites ◽  
Synthetic Hydroxyapatite ◽  
Polarized Raman Spectroscopy ◽  
Polarized Raman

In the Raman spectrum of B-type carbonated apatites, the ν1 CO32– mode (at ∼1070 cm–1) overlaps the ν3 PO43– band. The latter is readily observed where the CO32– content is low (up to ∼3 wt%). The CO32– content of bone is considerably higher (∼7–9 wt%). As a result, the ν3 PO43– band becomes completely obscured. The 1000–1100 cm–1 spectral range of carbonated apatite is frequently considered a combined ν3 PO43– and ν1 CO32– region. Here, high-resolution polarized Raman spectroscopy (step size of 0.74 ± 0.04 cm–1) provides new insights into synthetic hydroxyapatite (HAp) obtained as micrometer-sized fibers. Compared to bone mineral (deproteinized bovine bone), spectral features of HAp fibers are highly resolved. In particular, the ν3 PO43– band resolves into nine distinct sub-components: 1028, 1032, 1040, 1043, 1047, 1053, 1055, 1062, and 1076 cm–1. Parameters including full width half-maximum, intensity, area fraction, intensity ratio, and area fraction ratio vary between parallel and perpendicular polarized configurations. It is likely that the ν1 CO32– band of B-type carbonated apatites may contain a small but not insignificant contribution from the 1076 cm–1 sub-component of the ν3 PO43– band. Furthermore, the 1076 cm–1/1047 cm–1 ratio changes between parallel and perpendicular scattering configurations, suggesting that the contribution of the 1076 cm–1 sub-component may vary as a function of local orientation of bone mineral, thus skewing the ν1 CO32– band and compromising accurate estimation of carbonate-to-phosphate ratios in B-type CO32– substituted apatite.

scholarly journals Raman Spectroscopy detects changes in Bone Mineral Quality and Collagen Cross-linkage in Staphylococcus Infected Human Bone

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mohamed Khalid ◽  
Tanujjal Bora ◽  
Ahmed Al Ghaithi ◽  
Sharanjit Thukral ◽  
Joydeep Dutta
Keyword(s):  
Raman Spectroscopy ◽  
Bone Mineral ◽  
Human Bone ◽  
Cross Linkage
Sign in / Sign up

Export Citation Format

Share Document