New Strategy for Enhancement Curdlan Biosynthesis in Alcaligenes faecalis by Activating Gene Expression

2010 ◽  
Author(s):  
Hongtao Zhang ◽  
Xiaobei Zhan ◽  
Zhiyong Zheng ◽  
Jianrong Wu ◽  
Dingqiang Chen
Keyword(s):  
Gene Expression ◽  
Alcaligenes Faecalis ◽  
New Strategy

scholarly journals Asymmetric siRNA: New Strategy to Improve Specificity and Reduce Off-Target Gene Expression

2012 ◽  
Vol 23 (5) ◽  
pp. 521-532 ◽  
Author(s):  
Zhongping Yuan ◽  
Xilin Wu ◽  
Chao Liu ◽  
Genxing Xu ◽  
Zhiwei Wu
Keyword(s):  
Gene Expression ◽  
Target Gene ◽  
Target Gene Expression ◽  
New Strategy

scholarly journals A network view of microRNA and gene interactions in different pathological stages of colon cancer

2019 ◽  
Vol 12 (S7) ◽  
Author(s):  
Jia Wen ◽  
Benika Hall ◽  
Xinghua Shi
Keyword(s):  
Gene Expression ◽  
Colon Cancer ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Clinical Information ◽  
The Cancer Genome Atlas ◽  
Gene Interactions ◽  
New Strategy ◽  
Pathological Stages

Abstract Background Colon cancer is one of the common cancers in human. Although the number of annual cases has decreased drastically, prognostic screening and translational methods can be improved. Hence, it is critical to understand the molecular mechanisms of disease progression and prognosis. Results In this study, we develop a new strategy for integrating microRNA and gene expression profiles together with clinical information toward understanding the regulation of colon cancer. Particularly, we use this approach to identify microRNA and gene expression networks that are specific to certain pathological stages. To demonstrate the application of our method, we apply this approach to identify microRNA and gene interactions that are specific to pathological stages of colon cancer in The Cancer Genome Atlas (TCGA) datasets. Conclusions Our results show that there are significant differences in network connections between miRNAs and genes in different pathological stages of colon cancer. These findings point to a hypothesis that these networks signify different roles of microRNA and gene regulation in the pathogenesis and tumorigenesis of colon cancer.

Cytokine Gene Expression and IgA Production in the Spleen of Chickens Infected with Eimeria tenella

2020 ◽  
Author(s):  
Liushu Jia ◽  
Bianhua Zhou ◽  
Hongwei Wang ◽  
Fan Yang ◽  
Guoyong Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Morphological Characteristics ◽  
Eimeria Tenella ◽  
Cytokine Gene Expression ◽  
Control Group ◽  
Expression Levels ◽  
New Strategy ◽  
Iga Production ◽  
Mrna Expression Levels

To explore the effect of Eimeria tenella infection on the cytokines gene expression and IgA production in the spleen of chickens, the morphological characteristics of the spleen were observed through optical and transmission electron microscopy. The IgA production was determined through immunohistochemistry. The mRNA expression levels of splenic cytokines were detected through real-time PCR. Compared to the control group, along with the infection of E. tenella, the splenic lymphocytes exhibited irregular and cracked membranes, mitochondria swelled even vacuolization, the IgA expression in spleen tissue was decreased by 55.57% (p lessthan 0.01). Likewise, the mRNA expression levels of IL-2 and IL-1â decreased by 40% (plessthan 0.01) and 43% p lessthan 0.05), respectively. By contrast, the IL-6, IFN-g and IL-10 levels increased by 158% (p lessthan 0.01), 464% (p lessthan 0.05) and 379% p lessthan 0.01), respectively. These results indicated that the spleen implement an important function in the antagonism of E. tenella, which suggest a new strategy to control coccidiosis by improving the peripheral immunity of chickens.

scholarly journals Gene expression analysis of Alcaligenes faecalis during induction of heterotrophic nitrification

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shuhei Tsujino ◽  
Hideo Dohra ◽  
Taketomo Fujiwara
Keyword(s):  
Gene Expression ◽  
Growth Phase ◽  
Exponential Growth ◽  
Alcaligenes Faecalis ◽  
Heterotrophic Nitrification ◽  
Exponential Growth Phase ◽  
Primary Metabolism ◽  
Valuable Insight ◽  
Gene Encoding ◽  
Expression Levels

AbstractAlcaligenes faecalis is a heterotrophic nitrifying bacterium that oxidizes ammonia and generates nitrite and nitrate. When A. faecalis was cultivated in a medium containing pyruvate and ammonia as the sole carbon and nitrogen sources, respectively, high concentrations of nitrite accumulated in the medium whose carbon/nitrogen (C/N) ratio was lower than 10 during the exponential growth phase, while the accumulation was not observed in the medium whose C/N ratio was higher than 15. Comparative transcriptome analysis was performed using nitrifying and non-nitrifying cells of A. faecalis cultivated in media whose C/N ratios were 5 and 20, respectively, to evaluate the fluctuations of gene expression during induction of heterotrophic nitrification. Expression levels of genes involved in primary metabolism did not change significantly in the cells at the exponential growth phase under both conditions. We observed a significant increase in the expression levels of four gene clusters: pod cluster containing the gene encoding pyruvic oxime dioxygenase (POD), podh cluster containing the gene encoding a POD homolog (PODh), suf cluster involved in an iron-sulfur cluster biogenesis, and dnf cluster involved in a novel hydroxylamine oxidation pathway in the nitrifying cells. Our results provide valuable insight into the biochemical mechanism of heterotrophic nitrification.

scholarly journals A New Strategy to Identify and Annotate Human RPE-Specific Gene Expression

PLoS ONE ◽  
2010 ◽  
Vol 5 (5) ◽  
pp. e9341 ◽  
Author(s):  
Judith C. Booij ◽  
Jacoline B. ten Brink ◽  
Sigrid M. A. Swagemakers ◽  
Annemieke J. M. H. Verkerk ◽  
Anke H. W. Essing ◽  
...  
Keyword(s):  
Gene Expression ◽  
Specific Gene ◽  
Specific Gene Expression ◽  
New Strategy

scholarly journals A novel mice model of acute flares in osteoarthritis elicited by intra-articular injection of cultured mast cells

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Junpei Dan ◽  
Masashi Izumi ◽  
Hiroko Habuchi ◽  
Osami Habuchi ◽  
Shogo Takaya ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mast Cell ◽  
Mast Cells ◽  
Mice Model ◽  
Inhibitory Effects ◽  
Histological Changes ◽  
New Strategy ◽  
Monosodium Iodoacetate ◽  
Disease Modifying Treatment

Abstract Purpose Mast cells are multifunctional in osteoarthritis (OA), and infiltration of activated mast cells likely contributes to disease severity and progression. However, the detailed mechanisms of action are unclear. The purpose of this study was to elucidate the role of mast cell infiltration in OA at histological level using a new mice model and to investigate pharmacological inhibitory effects of existing mast cell stabilizers in this model. Methods Mice were injected intra-articularly with monosodium iodoacetate (MIA 0.5 mg) or PBS on day 0, and PBS, with or without mast cells (MC: 1 × 106 cells) on day 14. They were divided into four groups: OA flare (MIA + MC), OA (MIA + PBS), MC non-OA (PBS + MC), and PBS non-OA (PBS + PBS). In OA flare, the MC stabilizer drug (tranilast: 400 mg/kg/day) or PBS was administered intraperitoneally from days 15 to 21. Results Histologically, modified Mankin score of the OA flare was significantly higher than that of OA (7.0 [1.8] vs. 3.3 [1.3], P < 0.05), and a larger number of mast cells was observed in OA flare than in OA (34.5 [6.3]/mm2 vs. 27.2 [2.3]/mm2, P < 0.05) on day 22. OA flare also showed acute exacerbation of pain and increased gene expression of pro-inflammatory cytokines and aggrecanase compared with OA. Administration of tranilast to OA flare-up provoked significant improvements in term of histological changes, pain, and gene expression at day 22. Conclusion Our novel model possibly mimics OA flare conditions, which may open a new strategy of disease-modifying treatment for OA, focused on controlling the multiple functions of mast cells.

A modified GLGI method for identification of novel porcine genes from long serial analysis of gene expression tags

2007 ◽  
Vol 4 (2) ◽  
pp. 111-115 ◽  
Author(s):  
Tang Zhong-Lin ◽  
Li Yong ◽  
Zhao Shu-Hong ◽  
Liu Bang ◽  
Fan Bin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expressed Sequence Tags ◽  
Pcr Amplification ◽  
Est Sequencing ◽  
Cloning Strategy ◽  
New Strategy ◽  
Sense Primer ◽  
Polyadenylation Signals ◽  
Expressed Sequence ◽  
Cdna Fragments

AbstractCombining the long serial analysis of gene expression (LongSAGE) and the generation of longer cDNA fragments from serial analysis of gene expression tags for gene identification (GLGI) technique, a new strategy called modified GLGI (M-GLGI) was developed to isolate unknown 3′ expressed sequence tags (ESTs) and discover novel genes. A 17 bp LongSAGE tag was used as sense primer instead of a 10-base SAGE tag; PCR reaction was performed under an appropriate annealing temperature for each tag; universal DNA polymerase was used in PCR amplification instead of Pfu enzyme; a common cloning strategy using pMD-18T vector and Escherichia coli DH5α cells were used instead of a special vector and competent cells. Moreover, ESTs isolated by M-GLGI had 3′ ends with the polyadenylation signals and poly(dA) tails. This method is more sensitive for identifying genes expressed in low abundance than conventional EST sequencing.

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