Management of EMC semi-anechoic facilities by an independent laboratory

Author(s):  
G. Kiemel
2018 ◽  
Vol 3 (3) ◽  
pp. 397-407
Author(s):  
Yu Zi Zheng ◽  
Dustin R Bunch ◽  
Katherine Lembright ◽  
Sihe Wang

Abstract Background Benzodiazepines (BZDs) are central nervous system depressants that are prescribed to prevent seizures, manage anxiety, or help sleep. When misused, BZDs can lead to addiction and sometimes cause death. Measurement of BZDs in urine is used to identify their use, especially in pain management settings. LC-MS/MS is preferred for these measurements because of its high sensitivity and specificity. Here, we report an LC-MS/MS assay for measuring 7 BZDs and metabolites in urine. Methods Urine sample was incubated at 60 °C for 30 min after addition of internal standards and a β-glucuronidase solution. After centrifugation, the supernatant was diluted with methanol and water before being injected onto a C18 analytical column in an LC-MS/MS system for quantification. The analytical time between injections was 4.35 min. The analytes included 7-aminoclonazepam, α-hydroxyalprazolam, α-hydroxytriazolam, oxazepam, lorazepam, nordiazepam, and temazepam. Results The lower limit of quantification ranged from 30 ng/mL to 50 ng/mL with an analytical recovery >80% for all 7 analytes. Total CV was <10% for all analytes (3 concentration levels of 100, 2500, and 5000 ng/mL; n = 30 each). This method had 100% agreement with a GC-MS method offered by an independent laboratory for negative urine samples. For the positive urine samples, this method showed a strong correlation (R > 0.96) with the GC-MS method. Conclusions The LC-MS/MS assay allows accurate and precise measurement of 7 BZDs and metabolites in a single analytical run with a short analytical run time and broad measuring ranges.


1976 ◽  
Vol 54 (6) ◽  
pp. 825-842 ◽  
Author(s):  
Donald J. McQueen

Field data from a demographic study of the terrestrial isopod Porcellio spinicornis Say were analyzed and compared with laboratory data reported by McQueen and Carnio. On the study area, which covered 482.8 m2, it was observed that reproduction began when average temperatures were > 13 °C, and that about 86% of the individuals produced one brood and about 30% produced two broods. During 1972, 25 654 offspring were produced and during 1973, 27 664 offspring resulted from reproduction, which took place during May, June, July, and August. By October of both years, these animals attained sizes ranging from 10 to 30 mg and exhibited 42 to 47% survivorship. During the next summer, females grew to between 30 and 50 mg and males to between 20 and 40 mg, and survivorship ranged from 15% in May to 2% in August. During the third summer, females attained sizes ranging from 80 to 100 mg and males from 40 to 70 mg. Survivorship during this period ranged from 1% in May to 0% in August. All rates of growth, reproduction, and survival, as well as reproductive periodicity, agreed with independent laboratory studies conducted under controlled conditions. During the 3 years studied the field population remained relatively constant and appeared to be limited by temperature conditions.


1986 ◽  
Vol 2 (3) ◽  
pp. 267-268
Author(s):  
Suzanne M. Selvaggi ◽  
Leonard Japko

2000 ◽  
Vol 83 (6) ◽  
pp. 1349-1356 ◽  
Author(s):  
Alan M Hochberg ◽  
Paul N M Gerhardt ◽  
Thoa K Cao ◽  
Wilfredo Ocasio ◽  
W Mark Barbour ◽  
...  

Abstract An independent laboratory study of the BAX® for Screening/E. coli O157:H7 kit was conducted at the National Food Laboratory, Inc., Dublin, CA, to complete AOAC® Performance Tested MethodSM certification. The BAX system kit was compared with the BAM culture method and a modified BAM culture method for detection of E. coli O157:H7 in ground beef. The BAX system kit detected the target organism at levels approximately 10-fold lower than those that gave positive BAM results. This study validated product claims, and Performance Tested Method status was granted.


2011 ◽  
Vol 94 (5) ◽  
pp. 1519-1530
Author(s):  
Arthur Trombley ◽  
Titan Fan ◽  
Robert LaBudde

Abstract The level of total aflatoxin contamination was analyzed in naturally contaminated and spiked samples of corn and peanut using the Aflatoxin Plate Kit. This kit is an enzyme-linked immunosorbent assay (ELISA) suitable for rapid testing of grains and peanuts. The assay was evaluated for ruggedness and linearity of the standard curve. The test kit results were then statistically evaluated for accuracy, precision, and correlation to a validated HPLC method (AOAC 994.08). The results were verified by an independent laboratory.


2009 ◽  
Vol 92 (2) ◽  
pp. 449-458 ◽  
Author(s):  
Susan Alles ◽  
Linda X Peng ◽  
Mark A Mozola

Abstract A modification to Performance-Tested MethodSM (PTM) 070601, Reveal® Listeria Test (Reveal), is described. The modified method uses a new media formulation, LESS enrichment broth, in single-step enrichment protocols for both foods and environmental sponge and swab samples. Food samples are enriched for 2730 h at 30C and environmental samples for 2448 h at 30C. Implementation of these abbreviated enrichment procedures allows test results to be obtained on a next-day basis. In testing of 14 food types in internal comparative studies with inoculated samples, there was a statistically significant difference in performance between the Reveal and reference culture U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA/BAM) or U.S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) methods for only a single food in one trial (pasteurized crab meat) at the 27 h enrichment time point, with more positive results obtained with the FDA/BAM reference method. No foods showed statistically significant differences in method performance at the 30 h time point. Independent laboratory testing of 3 foods again produced a statistically significant difference in results for crab meat at the 27 h time point; otherwise results of the Reveal and reference methods were statistically equivalent. Overall, considering both internal and independent laboratory trials, sensitivity of the Reveal method relative to the reference culture procedures in testing of foods was 85.9 at 27 h and 97.1 at 30 h. Results from 5 environmental surfaces inoculated with various strains of Listeria spp. showed that the Reveal method was more productive than the reference USDA-FSIS culture procedure for 3 surfaces (stainless steel, plastic, and cast iron), whereas results were statistically equivalent to the reference method for the other 2 surfaces (ceramic tile and sealed concrete). An independent laboratory trial with ceramic tile inoculated with L. monocytogenes confirmed the effectiveness of the Reveal method at the 24 h time point. Overall, sensitivity of the Reveal method at 24 h relative to that of the USDA-FSIS method was 153. The Reveal method exhibited extremely high specificity, with only a single false-positive result in all trials combined for overall specificity of 99.5.


2014 ◽  
Vol 97 (5) ◽  
pp. 1374-1383 ◽  
Author(s):  
Debra Foti ◽  
Lei Zhang ◽  
Preetha Biswas ◽  
Mark Mozola ◽  
Jennifer Rice

Abstract A matrix extension study was conducted to validate the ANSR®Salmonella method for use with pasteurized egg products. Four diverse egg product types were tested by the ANSR method and the U.S. Department of Agriculture's Microbiology Laboratory Guidebook reference culture procedure. There were no significant differences in the number of positive test portions by the two methods for any of the products examined. Independent laboratory testing of pasteurized liquid egg also found no significant difference in performance between the ANSR and reference culture procedures. There were no false-positive results obtained in either internal or independent laboratory testing. Inclusivity testing using a new enrichment medium specifically designed for use with pasteurized egg products produced 112 positive results out of 113 Salmonella spp. strains tested, with only a single strain of S. Weslaco testing negative by the ANSR assay. Exclusivity testing of 38 non-salmonellae produced all negative ANSR results. It is concluded that ANSR Salmonella is a reliable, sensitive, and specific method for detection of Salmonella spp. in pasteurized egg products.


2017 ◽  
Vol 846 (2) ◽  
pp. 168 ◽  
Author(s):  
Steffen Spieler ◽  
Martin Kuhn ◽  
Johannes Postler ◽  
Malcolm Simpson ◽  
Roland Wester ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document