Shape Retaining and Sacrificial Molding Fabrication Method for ECM-Based in vitro Vascular Model

In this study, poly(∊-caprolactone) (PCL), gelatin (GEL) and nanocrystalline hydroxyapatite (HAp) was applied to fabricate novel PCL-GEL-HAp nanaocomposite scaffolds through a new fabrication method. With the aim of finding the best fabrication method, after testing different methods and solvents, the best method and solvents were found, and the nanocomposites were prepared through layer solvent casting combined with freeze-drying. Acetone and distillated water were used as the PCL and GEL solvents, respectively. The mechanical test showed that the increasing of the PCL weight through the scaffolds caused the improvement of the final nanocomposite mechanical behavior due to the increasing of the ultimate stress, stiffness and elastic modulus (8 MPa for 0% wt PCL to 23.5 MPa for 50% wt PCL). The biomineralization investigation of the scaffolds revealed the formation of bone-like apatite layers after immersion in simulated body fluid (SBF). In addition, the in vitro cytotoxity of the scaffolds using L929 mouse fibroblast cell line (ATCC) indicated no sign of toxicity. These results indicated that the fabricated scaffold possesses the prerequisites for bone tissue engineering applications.

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Mimicking arterial thrombosis in a 3D-printed microfluidic in vitro vascular model based on computed tomography angiography data

Lab on a Chip ◽

10.1039/c7lc00202e ◽

2017 ◽

Vol 17 (16) ◽

pp. 2785-2792 ◽

Cited By ~ 47

Author(s):

Pedro F. Costa ◽

Hugo J. Albers ◽

John E. A. Linssen ◽

Heleen H. T. Middelkamp ◽

Linda van der Hout ◽

...

Keyword(s):

Computed Tomography ◽

Computed Tomography Angiography ◽

Arterial Thrombosis ◽

Blood Clotting ◽

Microfluidic Chips ◽

Vascular Model ◽

Model Based ◽

3D Printed ◽

Vascular Structures

Studying blood clotting in stereolithography 3D-printed microfluidic chips with endothelialised vascular structures.

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Training in Neurovascular Intervention Usefulness of in-Vitro Model and Clinical Practice

Interventional Neuroradiology ◽

10.1177/15910199040100s118 ◽

2004 ◽

Vol 10 (1_suppl) ◽

pp. 107-112 ◽

Cited By ~ 7

Author(s):

K. Sugiu ◽

K. Tokunaga ◽

W. Sasahara ◽

K. Watanabe ◽

A. Nishida ◽

...

Keyword(s):

Clinical Training ◽

Vascular Lesions ◽

Clinical Experiences ◽

Vascular Model ◽

Stable Conditions ◽

Haemodynamic Changes ◽

Vitro Model ◽

Venous Fistula ◽

Clinical Conditions

We introduce our training tools and system of neurovascular intervention. An in vitro cerebral vascular model was used for the young residents to understand the basic interventional techniques and devices. The model included several vascular lesions such as cerebral aneurysm, dural arterio-venous fistula, or carotid artery stenosis. Endovascular procedures in the model were performed under fluoroscopic or direct visual control, and consecutive haemodynamic changes were visualized by using digital subtraction angiography and direct observation. Thus, traineess could have an easy understanding of clinical conditions. New medical devices, such as platinum coils, were successfully implanted in the model under stable conditions. After the initial training using vascular model, the residents had started clinical experiences under the control of senior surgeons. Although it is difficult to describe usefulness of our clinical training, we believe that we provide enough good quality and quantity of clinical cases to the residents. Because our endovascular team has recently had 150–200 interventional procedures every year, one resident can have experienced more than 100 cases per year. The qualification of a Board Certified Specialist of the Japanese Society of Intravascular Neurosurgery (JSIN) requires that the applicant must have experienced more than 100 cases for four years. So our residents can have enough case materials to qualify the board examination.

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Visualization of mechanical stimulation and cellular response in an in vitro vascular model

The Proceedings of the Symposium on Micro-Nano Science and Technology ◽

10.1299/jsmemnm.2019.10.20am2pn303 ◽

2019 ◽

Vol 2019.10 (0) ◽

pp. 20am2PN303

Author(s):

Kenya Hashimoto ◽

Azusa Shimizu ◽

Shigenori Miura ◽

Hiroaki Onoe

Keyword(s):

Mechanical Stimulation ◽

Cellular Response ◽

Vascular Model

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A bilayer small diameter in vitro vascular model for evaluation of drug induced vascular injury

Biomicrofluidics ◽

10.1063/1.4964814 ◽

2016 ◽

Vol 10 (5) ◽

pp. 054116 ◽

Cited By ~ 1

Author(s):

David M. Hoganson ◽

Eric B. Finkelstein ◽

Gwen E. Owens ◽

James C. Hsiao ◽

Kurt Y. Eng ◽

...

Keyword(s):

Vascular Injury ◽

Small Diameter ◽

Drug Induced ◽

Vascular Model

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Patient-Specific IVR Endovascular Simulator with Augmented Reality for Medical Training and Robot Evaluation

Journal of Robotics and Mechatronics ◽

10.20965/jrm.2008.p0441 ◽

2008 ◽

Vol 20 (3) ◽

pp. 441-448 ◽

Cited By ~ 13

Author(s):

Seiichi Ikeda ◽

◽

Carlos Tercero Villagran ◽

Toshio Fukuda ◽

Yuta Okada ◽

...

Keyword(s):

Augmented Reality ◽

Blood Vessel ◽

Medical Training ◽

Refraction Index ◽

Endovascular Intervention ◽

Patient Specific ◽

Image Subtraction ◽

Vascular Model ◽

Behavior Simulation

Endovascular intervention using interventional radiology (IVR) is most commonly used in cerebralvascular treatment. Medical imaging such as digital subtraction angiography (DSA) and vascular mapping make vasculature and catheters easier to read from fluoroscopy during endovascular intervention. We propose simulating IVR using augmented reality, reproducing fluoroscopic images and a patient-specific blood vessel model without X-ray imaging. The advantages of the patient-specific vascular model reproducing the human vasculature lumen with 13 μm resolution include 1) a realistic “feel,” 2) excellent tool behavior simulation during intervention, and 3) surgical training alternative to physician training in-vitro. Simulated fluoroscopic images are created in two steps: First, the blood vessel model refraction index is matched to surrounding glycerin solution to conceal the vascular model, making the silicone vasculature appear human as seen in endovascular intervention. Second, an augmented reality (AR) environment is created using image subtraction and overlap, making model-based endovascular simulation more understandable for catheter use and fluoroscopy use and reading.

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A patient-specific intracranial aneurysm model with endothelial lining: a novel in vitro approach to bridge the gap between biology and flow dynamics

Journal of NeuroInterventional Surgery ◽

10.1136/neurintsurg-2017-013087 ◽

2017 ◽

Vol 10 (3) ◽

pp. 306-309 ◽

Cited By ~ 23

Author(s):

Naoki Kaneko ◽

Toshihiro Mashiko ◽

Katsunari Namba ◽

Satoshi Tateshima ◽

Eiju Watanabe ◽

...

Keyword(s):

Endothelial Cells ◽

In Vitro Model ◽

Patient Specific ◽

Parent Artery ◽

Complex Flow ◽

Endothelial Lining ◽

Vascular Model ◽

Vitro Model ◽

Aneurysm Model

ObjectivesTo develop an in vitro model for studying the biological effect of complex-flow stress on endothelial cells in three-dimensional (3D) patient-specific vascular geometry.Materials and methodsA vessel replica was fabricated with polydimethylsiloxanes using 3D printing technology from vascular image data acquired by rotational angiography. The vascular model was coated with fibronectin and immersed in a tube filled with a cell suspension of endothelium, and then cultured while being slowly rotated in three dimensions. Culture medium with viscosity was perfused in the circulation with the endothelialized vascular model. A computational fluid dynamics (CFD) study was conducted using perfusion conditions used in the flow experiment. The morphology of endothelial cells was observed under a confocal microscope.ResultsThe CFD study showed low wall shear stress and circulating flow in the apex of the basilar tip aneurysm, with linear flow in the parent artery. Confocal imaging demonstrated that the inner surface of the vascular model was evenly covered with monolayer endothelial cells. After 24 h of flow circulation, endothelial cells in the parent artery exhibited a spindle shape and aligned with the flow direction. In contrast, endothelial cells in the aneurysmal apex were irregular in shape and size.ConclusionsA geometrically realistic intracranial aneurysm model with live endothelial lining was successfully developed. This in vitro model enables a new research approach combining study of the biological impact of complex flow on endothelial cells with CFD analysis and patient information, including the presence of aneurysmal growth or rupture.

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A review of application of natural products as fungicides for chili

Environmental and Toxicology Management ◽

10.33086/etm.v1i2.2022 ◽

2021 ◽

Vol 1 (2) ◽

pp. 9-22

Author(s):

Khoirul Ngibad ◽

Afidatul Muadifah ◽

Lailatul Jannah Triarini ◽

Laily Rizki Amalia ◽

Novita Karel Damayanti

Keyword(s):

Public Health ◽

Leaf Extract ◽

In Vitro Tests ◽

Fabrication Method ◽

Chromolaena Odorata ◽

Leaf Extracts ◽

In Vivo Test ◽

Fractionation Method

Anthracnose disease in chillies is a serious problem for farmers. So far, synthetic fungicides have been used as solution for the treatment of this disease. However, the side effects of synthetic fungicides to public health and environment raised awareness on alternative fungicides derived from natural resources. This paper aims to review plants that are potential as an alternative to fungicides for chili plantation, fabrication of test solutions, in vitro and in vivo fungicide test. Many plants were investigated as alternatives to plant-based fungicide. The utilization of leaves as samples including rhizomes, roots, tubers, weevils, seeds, fruit, flowers and other parts of the plant. The extract fabrication method used as a fungicide test include: maceration method, gradual fractionation method, and decoction method. The maceration method is the method most widely used to extract fungicidal active compounds from plants. Some studies that carried out in vitro tests were unable to compare with synthetic fungicides so it was not possible to determine their effectiveness for plant-based fungicide for chillies when compared to synthetic fungicides. In vitro Extract of 80% alcohol and 10%/60% n-hexane of pacar cina (Aglaia odorata L.) leaves can be compared with the performance of propineb 0.2%. In addition, the 60% and 70% kirinyuh (Chromolaena odorata L.) leaf extracts were also able to match Acrobat 0.2% performance in vitro. Based on the in vivo test, suren (Toona sureni Merr) leaf extract and nut bulbs can be used as an alternative to vegetable / natural fungicides to help overcome the problem of anthracnose in chilies.

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