Introducing novel mimic materials as alternatives for natural enzymes challenges the analysts. Study on the peroxidase-like materials is an active field in analytical research areas. Herein, Au/Cu bimetal nanoclusters (Au/Cu NCs) are introduced as highly efficient peroxidase mimics, which were investigated using fluorometric and colorimetric techniques. A comprehensive comparison between the catalytic activity of Au, Cu, and their bimetal NCs, with different ratios of Au/Cu was performed using some different peroxidase substrates (including 3,3′,5,5′-tetramethylbenzidine (TMB), o-phenylenediamine dihydrochloride (OPD), and terephthalic acid (TA)). Additionally, different capping agents were applied for the synthesis of NCs, and it was found that penicillamine-capped NCs with 50% Cu have higher activity than other synthesized NCs. Analytical application of the novel mimic for H2O2 detection caused a linear calibration in a wide linear range of 0.001–3 μmol/L, and a great detection limit (3S) of 0.18 nmol/L, using a sensitive fluorescence system. The developed system was also sensitive for recognizing glucose and cholesterol in blood samples, after their enzymatic oxidation and production of H2O2. Detection limits of 55 and 15 nmol/L were obtained for glucose and cholesterol, respectively. The presented method also showed good reliability, which was validated by certified reference materials.
Deep Reinforcement Learning: A State-of-the-Art Walkthrough