P45: Glucose stimulates Streptococcus agalactiae biofilm formation and promotes  in vitro vaginal cell colonization

2021 ◽  
Vol 85 (S1) ◽  
pp. 84-84
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Cell Colonization

scholarly journals In -vitro Biofilm Formation of Vaginal Isolates of Streptococcus agalactiae; Effect of pH and Culture Media

2021 ◽  
Vol 64 ◽  
Author(s):  
Guruge Niluka Dilrukshi ◽  
Jananie Kottahachchi ◽  
Thushari Dissanayake ◽  
Manjula Weerasekera ◽  
Mudara Peiris ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Culture Media ◽  
Effect Of Ph

scholarly journals Acidic pH Strongly EnhancesIn VitroBiofilm Formation by a Subset of Hypervirulent ST-17 Streptococcus agalactiae Strains

2014 ◽  
Vol 80 (7) ◽  
pp. 2176-2185 ◽  
Author(s):  
Nunzia D'Urzo ◽  
Manuele Martinelli ◽  
Alfredo Pezzicoli ◽  
Virginia De Cesare ◽  
Vittoria Pinto ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Medium Composition ◽  
Proteinase K ◽  
Neonatal Meningitis ◽  
Neonatal Infections ◽  
Content Type ◽  
Acidic Conditions ◽  
Group B

ABSTRACTStreptococcus agalactiae, also known as group BStreptococcus(GBS), is a primary colonizer of the anogenital mucosa of up to 40% of healthy women and an important cause of invasive neonatal infections worldwide. Among the 10 known capsular serotypes, GBS type III accounts for 30 to 76% of the cases of neonatal meningitis. In recent years, the ability of GBS to form biofilm attracted attention for its possible role in fitness and virulence. Here, a newin vitrobiofilm formation protocol was developed to guarantee more stringent conditions, to better discriminate between strong-, low-, and non-biofilm-forming strains, and to facilitate interpretation of data. This protocol was used to screen the biofilm-forming abilities of 366 GBS clinical isolates from pregnant women and from neonatal infections of different serotypes in relation to medium composition and pH. The results identified a subset of isolates of serotypes III and V that formed strong biofilms under acidic conditions. Importantly, the best biofilm formers belonged to serotype III hypervirulent clone ST-17. Moreover, the abilities of proteinase K to strongly inhibit biofilm formation and to disaggregate mature biofilms suggested that proteins play an essential role in promoting GBS biofilm initiation and contribute to biofilm structural stability.

scholarly journals Omadacycline efficacy against Streptococcus agalactiae isolated in China: in vitro activity, resistance analysis, clonality of MIC distribution, and the correlation between resistance and virulence gene and biofilm formation

2020 ◽  
Author(s):  
Guiqiu Li ◽  
Ying Wei ◽  
Yan Guo ◽  
Jie Lian ◽  
Shaoyuan Wu ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Biofilm Formation ◽  
Reproductive Tract ◽  
Venous Blood ◽  
Virulence Gene ◽  
Inhibition Zone ◽  
Resistance Rate ◽  
Erythromycin Resistance ◽  
Urethral Discharge

Abstract Purpose: This study aimed to evaluate activity，resistance, clonality of MIC distribution, and the correlation between virulence＆resistance gene and biofilm formation of omadacycline (OMC) in clinical for Streptococcus agalactiae isolates from China.Methods: 162 isolates were collected retrospectively in China from 2018 to 2019. The S. agalactiae were mainly collected from the body's cervical secretions, wound secretions, ear swab, secretions, semen, venous blood, cerebrospinal fluid, pee, urethral discharge, pus, umbilical secretions, wound secretions, reproductive tract secretions, sputum, gastric juice, throat swab, eye secretions and amniotic fluid. The MIC of OMC against S. agalactiae were determined by broth microdilution. Filter paper was used to measure the inhibition zone diameters of OMC and other common antibiotics. D-test detected the incidence of erythromycin resistance to inductively clindamycin. Multilocus sequence typing (MLST)，resistance＆virulence gene of the isolates were investigated using qRT-PCR. Biofilms were detected by crystal violet staining.Results: The OMC MIC of clinical S. agalactiae isolates ranged from ≤0.25 to 0.5 mg/L. 19.7% of the S. agalactiae isolates with an OMC MIC of 0.25 mg/L were found to be sequence type (ST) 17. The S. agalactiae had highly resistant to Minocyclin, Erytromycin, Solithromycin and Clindamycin and the resistance rate of OMA was 13.6%. The positive rate of D-test was 90.74 %. The formation of biofilm was related to scpB gene, and indicated the resistance of OMA may be related to the virulence gene scpB. Conclusion: OMC exhibited better activity against clinical S. agalactiae isolates from China than DOX or MIN, and scpB was related with biofilm formation in OMC- resistance S. agalactiae.

Bioactivity of Phenolic Compounds Extracted from Strawberry against Formation of Pseudomonas aeruginosa Biofilm

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Baydaa Hussein ◽  
Zainab A. Aldhaher ◽  
Shahrazad Najem Abdu-Allah ◽  
Adel Hamdan
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Phenolic Compounds ◽  
Biofilm Formation ◽  
Opportunistic Infections ◽  
Formation Rate ◽  
Hydrocarbon Chain ◽  
Health Concern ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Contents

Background: Biofilm is a bacterial way of life prevalent in the world of microbes; in addition to that it is a source of alarm in the field of health concern. Pseudomonas aeruginosa is a pathogenic bacterium responsible for all opportunistic infections such as chronic and severe. Aim of this study: This paper aims to provide an overview of the promotion of isolates to produce a biofilm in vitro under special circumstances, to expose certain antibiotics to produce phenotypic evaluation of biofilm bacteria. Methods and Materials: Three diverse ways were used to inhibited biofilm formation of P.aeruginosa by effect of phenolic compounds extracts from strawberries. Isolates produced biofilm on agar MacConkey under certain circumstances. Results: The results showed that all isolates were resistant to antibiotics except sensitive to azithromycin (AZM, 15μg), and in this study was conducted on three ways to detect the biofilm produced, has been detected by the biofilm like Tissue culture plate (TCP), Tube method (TM), Congo Red Agar (CRA). These methods gave a clear result of these isolates under study. Active compounds were analyzed in both extracts by Gas Chromatography-mass Spectrometry which indicate High molecular weight compound with a long hydrocarbon chain. Conclusion: Phenolic compounds could behave as bioactive material and can be useful to be used in pharmaceutical synthesis. Phenolic contents which found in leaves and fruits extracts of strawberries shows antibacterial activity against all strains tested by the ability to reduce the production of biofilm formation rate.

scholarly journals In VitroAnalyses of the Effects of Heparin and Parabens on Candida albicans Biofilms and Planktonic Cells

2011 ◽  
Vol 56 (1) ◽  
pp. 148-153 ◽  
Author(s):  
Marisa H. Miceli ◽  
Stella M. Bernardo ◽  
T. S. Neil Ku ◽  
Carla Walraven ◽  
Samuel A. Lee
Keyword(s):  
Candida Albicans ◽  
Metabolic Activity ◽  
Biofilm Formation ◽  
High Dose ◽  
Dependent Manner ◽  
Planktonic Cells ◽  
Content Type ◽  
Heparin Sodium ◽  
The Individual

ABSTRACTInfections and thromboses are the most common complications associated with central venous catheters. Suggested strategies for prevention and management of these complications include the use of heparin-coated catheters, heparin locks, and antimicrobial lock therapy. However, the effects of heparin onCandida albicansbiofilms and planktonic cells have not been previously studied. Therefore, we sought to determine thein vitroeffect of a heparin sodium preparation (HP) on biofilms and planktonic cells ofC. albicans. Because HP contains two preservatives, methyl paraben (MP) and propyl paraben (PP), these compounds and heparin sodium without preservatives (Pure-H) were also tested individually. The metabolic activity of the mature biofilm after treatment was assessed using XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction and microscopy. Pure-H, MP, and PP caused up to 75, 85, and 60% reductions of metabolic activity of the mature preformedC. albicansbiofilms, respectively. Maximal efficacy against the mature biofilm was observed with HP (up to 90%) compared to the individual compounds (P< 0.0001). Pure-H, MP, and PP each inhibitedC. albicansbiofilm formation up to 90%. A complete inhibition of biofilm formation was observed with HP at 5,000 U/ml and higher. When tested against planktonic cells, each compound inhibited growth in a dose-dependent manner. These data indicated that HP, MP, PP, and Pure-H havein vitroantifungal activity againstC. albicansmature biofilms, formation of biofilms, and planktonic cells. Investigation of high-dose heparin-based strategies (e.g., heparin locks) in combination with traditional antifungal agents for the treatment and/or prevention ofC. albicansbiofilms is warranted.

scholarly journals Extract from phyllosphere bacteria with antibiofilm and quorum quenching activity to control several fish pathogenic bacteria

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Olivia Nathalia ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Vibrio Harveyi ◽  
Quorum Quenching ◽  
Indicator Bacteria ◽  
Antibiofilm Activity ◽  
Fish Pathogen ◽  
Phyllosphere Bacteria

Abstract Objective The objective of this research were to screen quorum quenching activity compound from phyllosphere bacteria as well as antibiofilm activity against several fish pathogen bacteria such as Aeromonas hydrophila, Streptococcus agalactiae, and Vibrio harveyi. Results We found eight phyllosphere bacteria isolates with potential quorum quenching activity to inhibit Chromobacterium violaceum as indicator bacteria. Crude extracts (20 mg/mL) showed various antibiofilm activity against fish pathogenic bacteria used in this study. Isolate JB 17B showed the highest activity to inhibit biofilm formation of A. hydrophila and V. harveyi, meanwhile isolate JB 3B showed the highest activity to inhibit biofilm of S. agalactiae. From destruction assay, isolate JB 8F showed the highest activity to disrupt biofilm of A. hydrophila isolate JB 20B showed the highest activity to disrupt biofilm of V. harveyi, isolate JB 17B also showed the highest activity to disrupt biofilm of S. agalactiae.

scholarly journals Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential

2020 ◽  
Vol 14 (1) ◽  
pp. 24
Author(s):  
Nevena Lj. Stevanović ◽  
Ivana Aleksic ◽  
Jakob Kljun ◽  
Sanja Skaro Bogojevic ◽  
Aleksandar Veselinovic ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Biofilm Formation ◽  
Candida Parapsilosis ◽  
Therapeutic Potential ◽  
A549 Cells ◽  
Candida Krusei ◽  
Strong Inhibition ◽  
Subinhibitory Concentrations ◽  
Pyocyanin Production

Copper(II) and zinc(II) complexes with clinically used antifungal drug fluconazole (fcz), {[CuCl2(fcz)2].5H2O}n, 1, and {[ZnCl2(fcz)2]·2C2H5OH}n, 2, were prepared and characterized by spectroscopic and crystallographic methods. The polymeric structure of the complexes comprises four fluconazole molecules monodentately coordinated via the triazole nitrogen and two chlorido ligands. With respect to fluconazole, complex 2 showed significantly higher antifungal activity against Candida krusei and Candida parapsilosis. All tested compounds reduced the total amount of ergosterol at subinhibitory concentrations, indicating that the mode of activity of fluconazole was retained within the complexes, which was corroborated via molecular docking with cytochrome P450 sterol 14α-demethylase (CYP51) as a target. Electrostatic, steric and internal energy interactions between the complexes and enzyme showed that 2 has higher binding potency to this target. Both complexes showed strong inhibition of C. albicans filamentation and biofilm formation at subinhibitory concentrations, with 2 being able to reduce the adherence of C. albicans to A549 cells in vitro. Complex 2 was able to reduce pyocyanin production in Pseudomonas aeruginosa between 10% and 25% and to inhibit its biofilm formation by 20% in comparison to the untreated control. These results suggest that complex 2 may be further examined in the mixed Candida-P. aeruginosa infections.

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