The methanolic extract of Guibourtia tessmannii (caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement of TRPV 1 channels

Andrologia ◽  
2018 ◽  
pp. e13216 ◽  
Author(s):  
Patrick Brice Defo Deeh ◽  
Pierre Watcho ◽  
Modeste Wankeu‐Nya ◽  
Esther Ngadjui ◽  
Umar Zayyanu Usman
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
Methanolic Extract ◽  
Cytosolic Calcium ◽  
Calcium Accumulation ◽  
And Oxidative Stress

Capsicum annuum L. Methanolic Extract Inhibits Ovalbumin-Induced Airway Inflammation and Oxidative Stress in a Mouse Model of Asthma

2011 ◽  
Vol 14 (10) ◽  
pp. 1144-1151 ◽  
Author(s):  
Ha-Young Jang ◽  
Se-Mi Kim ◽  
Ji-Eun Yuk ◽  
Ok-Kyoung Kwon ◽  
Sei-Ryang Oh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Model ◽  
Airway Inflammation ◽  
Capsicum Annuum ◽  
Methanolic Extract ◽  
Capsicum Annuum L ◽  
And Oxidative Stress

Toxicity and oxidative stress induced by T-2 toxin in cultured mouse Leydig cells

2016 ◽  
Vol 27 (2) ◽  
pp. 100-106 ◽  
Author(s):  
Yong Fa Zhang ◽  
Jian Ying Yang ◽  
Yong Kai Li ◽  
Wei Zhou
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
And Oxidative Stress

Curcumin inhibits zearalenone-induced apoptosis and oxidative stress in Leydig cells via modulation of the PTEN/Nrf2/Bip signaling pathway

2020 ◽  
Vol 141 ◽  
pp. 111385 ◽  
Author(s):  
Si Chen ◽  
Shuhua Yang ◽  
Mingyang Wang ◽  
Jia Chen ◽  
Sheng Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Signaling Pathway ◽  
Leydig Cells ◽  
Induced Apoptosis ◽  
And Oxidative Stress

scholarly journals Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3084 ◽  
Author(s):  
Gaoqing Xu ◽  
Jing Zhao ◽  
Hongyu Liu ◽  
Jun Wang ◽  
Wenfa Lu
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
B Cell Lymphoma ◽  
Nuclear Antigen ◽  
Protective Effects ◽  
Mrna And Protein Expression ◽  
Silent Information Regulator 1 ◽  
Proliferating Cell ◽  
And Oxidative Stress ◽  
Dependent Mechanism

The purpose of the present study is to examine the effects of melatonin on apoptosis and oxidative stress in mouse Leydig cells and to elucidate the mechanisms responsible for these effects. Our results indicated that 10 ng/mL of melatonin significantly promoted cell viability, the ratio of EdU-positive (5-Ethynyl-2′-deoxyuridine) cells, and increased the mRNA expression of proliferating cell nuclear antigen (PCNA), cyclin D1(CCND1), and cell division control protein 42 (CDC42) (p < 0.05). We also observed that melatonin inhibited apoptosis of mouse Leydig cells, accompanied with increased B-cell lymphoma-2 (BCL-2) and decreased BCL2 associated X (BAX) mRNA and protein expression. Moreover, addition of melatonin significantly decreased the reactive oxygen species (ROS) production and malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, while it increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels (p < 0.05). In addition, we also found that melatonin increased the expression of SIRT1 (Silent information regulator 1) (p < 0.05). To explore the role of SIRT1 signaling in melatonin-induced cells, mouse Leydig cells were pretreated with EX527, an inhibitor of SIRT1. The protective effects of melatonin on mouse Leydig cells were reversed by EX527, as shown by decreased cell proliferation and increased cell apoptosis and oxidative stress. In summary, our results demonstrated that melatonin inhibited apoptosis and oxidative stress of mouse Leydig cells through a SIRT1-dependent mechanism.

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